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Didier Raoult - One of the best experts on this subject based on the ideXlab platform.
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three toed sloth as putative reservoir of coxiella burnetii cayenne french guiana
Emerging Infectious Diseases, 2014Co-Authors: Bernard Davoust, Jean-lou Marié, Vincent Pommier De Santi, Jean Michel Berenger, Sophie Edouard, Didier RaoultAbstract:To the Editor: Q fever is an emerging zoonosis and a major public health concern in French Guiana, a French overseas region located on the northeastern coast of South America (1,2). Most cases occur in the city of Cayenne (3), specifically in the suburbs, where houses are near wooded hills (4). Genotyping performed by using multispacer Sequence typing showed that MST17, a unique genotype of C. burnetii, circulates in Cayenne and is responsible for epidemics of Q fever (5). C. burnetii transmission peaks during the rainy season, and the incidence of Q fever usually increases 1–3 months later (6). The animal reservoir of C. burnetii in French Guiana is unknown; previous studies have excluded domestic ruminants, which are known to be C. burnetii reservoirs elsewhere in the world (6). Four serologic surveys showed few C. burnetii–positive opossums, dogs, rodents (Proechimys spp.), bovines, or birds in French Guiana (7). In 2013, using real-time PCR (qPCR) analysis of vaginal swab samples, we showed that 6/158 (3.8%) dogs from Cayenne and 0/206 bats from the coastal area of French Guiana were positive for C. burnetii (cycle threshold [Ct]<35). One of the positive samples was identified as genotype MST17 (5). A case–control study among humans identified several risk factors for Q fever, including living near a forest and the presence of wild animals near the house (6). During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne. Vaginal swab samples were collected from animals living in the area (13 goats, 8 sheep, 7 bats, 34 birds, 2 opossums, 4 iguanas, and 17 geckos); all samples were negative for C. burnetii by qPCR. In addition, serologic tests for C. burnetii were negative for samples from all 37 small ruminants maintained near the outbreak area. In January 2014, a dead (accidental death) female 3-toed sloth (Bradypus tridactylus) (Figure, panel A) was found on the road near the residence of a Q fever patient. We retrieved the sloth and collected feces, spleen, liver, kidney, lung, and uterus samples and a vaginal swab sample. A total of 16 ticks were removed from the sloth and stored in 70% alcohol. Figure Putative reservoir of Coxiella burnetii in Cayenne, French Guiana A) A 3-toed sloth (Bradypus tridactylus) in its natural habitat in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne, French Guiana (photograph by S. ... DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). qPCR targeting the Repeated insertion Sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8). We confirmed all positive results by performing a second qPCR targeting the IS30a Repeated Sequence. DNA samples with Ct values <35 in both assays were considered positive for C. burnetii. A standard calibration curve quantifying the target IS1111 was generated by using 10-fold serial dilutions of C. burnetii Nine Mile strain. The number of IS1111 intergenic Sequences found in the genome of strain C. burnetii MST17 was identical to that for the Nine Mile strain (F. D’Amato, unpub. data); thus, the qPCR that we used was valid for quantifying the number of C. burnetii MST17 IS1111 copies/mL in samples we collected (5). qPCR analysis showed that the feces were highly positive for C. burnetii; the sample had a low Ct value of 23, corresponding to 7 log10 DNA copies/mL (9). The spleen was also positive for C. burnetii; the Ct value was 34, corresponding to 3.6 log10 DNA copies/mL. Results for the other samples were negative. Using morphologic criteria, we identified all 16 ticks collected from the sloth as Amblyomma geayi (Figure, panel B). We performed C. burnetii–specific qPCR on the ticks; 14 (88%) were positive. We genotyped C. burnetii–positive DNA from the feces and from 6 of the 16 ticks by using multispacer Sequence typing as described (5). All samples were identified as MST17, the unique genotype circulating in Cayenne (5). After obtaining the laboratory results, we confirmed that a local group in charge of the collection and treatment of injured animals usually released rehabilitated 3-toed sloths into Tiger Camp. Residents of Tiger Camp regularly observed and came into contact with the sloths, and ticks were frequently observed on the fur of the animals. Furthermore, 3 Q fever patients from Cayenne reported contact with sloths. Feces from the sloth in this study were highly infectious for C. burnetii. Because sloths live in tall trees and can shed this bacterium in their feces, human contamination might occur through inhalation of infectious aerosols from feces. The high prevalence of C. burnetii infection in ticks also suggests possible transmission through tick bites or from aerosols of tick feces that have been deposited on the skin of animal hosts; such feces can be extremely rich in bacteria and highly infectious (10). In this 2013 outbreak of Q fever, epidemiologic studies led to the identification of 3-toed sloths as a putative source of C. burnetii infection. Further investigations are needed to confirm the role of sloths as a reservoir for C. burnetii in French Guiana and to implement efficient measures to prevent transmission to humans.
Bernard Davoust - One of the best experts on this subject based on the ideXlab platform.
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three toed sloth as putative reservoir of coxiella burnetii cayenne french guiana
Emerging Infectious Diseases, 2014Co-Authors: Bernard Davoust, Jean-lou Marié, Vincent Pommier De Santi, Jean Michel Berenger, Sophie Edouard, Didier RaoultAbstract:To the Editor: Q fever is an emerging zoonosis and a major public health concern in French Guiana, a French overseas region located on the northeastern coast of South America (1,2). Most cases occur in the city of Cayenne (3), specifically in the suburbs, where houses are near wooded hills (4). Genotyping performed by using multispacer Sequence typing showed that MST17, a unique genotype of C. burnetii, circulates in Cayenne and is responsible for epidemics of Q fever (5). C. burnetii transmission peaks during the rainy season, and the incidence of Q fever usually increases 1–3 months later (6). The animal reservoir of C. burnetii in French Guiana is unknown; previous studies have excluded domestic ruminants, which are known to be C. burnetii reservoirs elsewhere in the world (6). Four serologic surveys showed few C. burnetii–positive opossums, dogs, rodents (Proechimys spp.), bovines, or birds in French Guiana (7). In 2013, using real-time PCR (qPCR) analysis of vaginal swab samples, we showed that 6/158 (3.8%) dogs from Cayenne and 0/206 bats from the coastal area of French Guiana were positive for C. burnetii (cycle threshold [Ct]<35). One of the positive samples was identified as genotype MST17 (5). A case–control study among humans identified several risk factors for Q fever, including living near a forest and the presence of wild animals near the house (6). During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne. Vaginal swab samples were collected from animals living in the area (13 goats, 8 sheep, 7 bats, 34 birds, 2 opossums, 4 iguanas, and 17 geckos); all samples were negative for C. burnetii by qPCR. In addition, serologic tests for C. burnetii were negative for samples from all 37 small ruminants maintained near the outbreak area. In January 2014, a dead (accidental death) female 3-toed sloth (Bradypus tridactylus) (Figure, panel A) was found on the road near the residence of a Q fever patient. We retrieved the sloth and collected feces, spleen, liver, kidney, lung, and uterus samples and a vaginal swab sample. A total of 16 ticks were removed from the sloth and stored in 70% alcohol. Figure Putative reservoir of Coxiella burnetii in Cayenne, French Guiana A) A 3-toed sloth (Bradypus tridactylus) in its natural habitat in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne, French Guiana (photograph by S. ... DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). qPCR targeting the Repeated insertion Sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8). We confirmed all positive results by performing a second qPCR targeting the IS30a Repeated Sequence. DNA samples with Ct values <35 in both assays were considered positive for C. burnetii. A standard calibration curve quantifying the target IS1111 was generated by using 10-fold serial dilutions of C. burnetii Nine Mile strain. The number of IS1111 intergenic Sequences found in the genome of strain C. burnetii MST17 was identical to that for the Nine Mile strain (F. D’Amato, unpub. data); thus, the qPCR that we used was valid for quantifying the number of C. burnetii MST17 IS1111 copies/mL in samples we collected (5). qPCR analysis showed that the feces were highly positive for C. burnetii; the sample had a low Ct value of 23, corresponding to 7 log10 DNA copies/mL (9). The spleen was also positive for C. burnetii; the Ct value was 34, corresponding to 3.6 log10 DNA copies/mL. Results for the other samples were negative. Using morphologic criteria, we identified all 16 ticks collected from the sloth as Amblyomma geayi (Figure, panel B). We performed C. burnetii–specific qPCR on the ticks; 14 (88%) were positive. We genotyped C. burnetii–positive DNA from the feces and from 6 of the 16 ticks by using multispacer Sequence typing as described (5). All samples were identified as MST17, the unique genotype circulating in Cayenne (5). After obtaining the laboratory results, we confirmed that a local group in charge of the collection and treatment of injured animals usually released rehabilitated 3-toed sloths into Tiger Camp. Residents of Tiger Camp regularly observed and came into contact with the sloths, and ticks were frequently observed on the fur of the animals. Furthermore, 3 Q fever patients from Cayenne reported contact with sloths. Feces from the sloth in this study were highly infectious for C. burnetii. Because sloths live in tall trees and can shed this bacterium in their feces, human contamination might occur through inhalation of infectious aerosols from feces. The high prevalence of C. burnetii infection in ticks also suggests possible transmission through tick bites or from aerosols of tick feces that have been deposited on the skin of animal hosts; such feces can be extremely rich in bacteria and highly infectious (10). In this 2013 outbreak of Q fever, epidemiologic studies led to the identification of 3-toed sloths as a putative source of C. burnetii infection. Further investigations are needed to confirm the role of sloths as a reservoir for C. burnetii in French Guiana and to implement efficient measures to prevent transmission to humans.
M.r. Mott - One of the best experts on this subject based on the ideXlab platform.
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A study of electrophoretic mobility of DNA in agarose and polyacrylamide gels.
Journal of molecular biology, 1991Co-Authors: C. R. Calladine, C.m. Collis, Horace R. Drew, M.r. MottAbstract:Abstract The aim of this paper is to clarify the mechanisms of gel electrophoresis of DNA under constant-field conditions. We have conducted a large number of experiments on double-stranded DNA varying in length between ≈10 and ≈50,000 base-pairs, in both agariose and polyarcylamide gels ranging from 0·5% to 12% concentration, and with electric field strengths ranging from 0·5 to 8 V/cm. We have made (logarithmic) plots of velocity against length of DNA for all of the various test conditions. At the left-hand side of these plots, all of the empirical curves have a unique, standard shape. When the curves are normalized so that their left-hand parts coincide, a second feature emerges in that, while for any given test the curve follows the “master curve” up to a certain point, it then “breaks away” and becomes horizontal. We describe these two patterns of behaviour as “regions 1 and 2”, respectively. We find simple yet comprehensive empirical formulae that fit the observations in the two regions of behaviour: these express the velocity in terms of length of DNA, electric field strength and gel concentration. We then construct two separate theories for the two regions of behaviour. The first theory involves the statistics of motion of an object through a random array of gel obstacles, with the instantenous speed depending on the number of obstacles with which the objects is currently in contact. The second theory is based on the mechanical hypothesis (for which there is other, independent support) that the DNA moves through the gel by piling up against a barrier, which eventually breaks or deforms under the resulting force, thereby allowing the DNA to move on the next barrier. The statistical theory is an adaptation of existing work, while the mechanical one is new. We also describe experiments on the migration of Repeated-Sequence, curved DNA with length up to 1500 base-pairs, and we discuss its behaviour in terms of our two theories. Our studies by electron microscopy are consistent with the view that this Repeated-Sequence DNA adopts a superhelical configuration. Finally, we show that a very wide range of observations may be understood clearly by means of our two theoretical schemes.
Vincent Pommier De Santi - One of the best experts on this subject based on the ideXlab platform.
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three toed sloth as putative reservoir of coxiella burnetii cayenne french guiana
Emerging Infectious Diseases, 2014Co-Authors: Bernard Davoust, Jean-lou Marié, Vincent Pommier De Santi, Jean Michel Berenger, Sophie Edouard, Didier RaoultAbstract:To the Editor: Q fever is an emerging zoonosis and a major public health concern in French Guiana, a French overseas region located on the northeastern coast of South America (1,2). Most cases occur in the city of Cayenne (3), specifically in the suburbs, where houses are near wooded hills (4). Genotyping performed by using multispacer Sequence typing showed that MST17, a unique genotype of C. burnetii, circulates in Cayenne and is responsible for epidemics of Q fever (5). C. burnetii transmission peaks during the rainy season, and the incidence of Q fever usually increases 1–3 months later (6). The animal reservoir of C. burnetii in French Guiana is unknown; previous studies have excluded domestic ruminants, which are known to be C. burnetii reservoirs elsewhere in the world (6). Four serologic surveys showed few C. burnetii–positive opossums, dogs, rodents (Proechimys spp.), bovines, or birds in French Guiana (7). In 2013, using real-time PCR (qPCR) analysis of vaginal swab samples, we showed that 6/158 (3.8%) dogs from Cayenne and 0/206 bats from the coastal area of French Guiana were positive for C. burnetii (cycle threshold [Ct]<35). One of the positive samples was identified as genotype MST17 (5). A case–control study among humans identified several risk factors for Q fever, including living near a forest and the presence of wild animals near the house (6). During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne. Vaginal swab samples were collected from animals living in the area (13 goats, 8 sheep, 7 bats, 34 birds, 2 opossums, 4 iguanas, and 17 geckos); all samples were negative for C. burnetii by qPCR. In addition, serologic tests for C. burnetii were negative for samples from all 37 small ruminants maintained near the outbreak area. In January 2014, a dead (accidental death) female 3-toed sloth (Bradypus tridactylus) (Figure, panel A) was found on the road near the residence of a Q fever patient. We retrieved the sloth and collected feces, spleen, liver, kidney, lung, and uterus samples and a vaginal swab sample. A total of 16 ticks were removed from the sloth and stored in 70% alcohol. Figure Putative reservoir of Coxiella burnetii in Cayenne, French Guiana A) A 3-toed sloth (Bradypus tridactylus) in its natural habitat in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne, French Guiana (photograph by S. ... DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). qPCR targeting the Repeated insertion Sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8). We confirmed all positive results by performing a second qPCR targeting the IS30a Repeated Sequence. DNA samples with Ct values <35 in both assays were considered positive for C. burnetii. A standard calibration curve quantifying the target IS1111 was generated by using 10-fold serial dilutions of C. burnetii Nine Mile strain. The number of IS1111 intergenic Sequences found in the genome of strain C. burnetii MST17 was identical to that for the Nine Mile strain (F. D’Amato, unpub. data); thus, the qPCR that we used was valid for quantifying the number of C. burnetii MST17 IS1111 copies/mL in samples we collected (5). qPCR analysis showed that the feces were highly positive for C. burnetii; the sample had a low Ct value of 23, corresponding to 7 log10 DNA copies/mL (9). The spleen was also positive for C. burnetii; the Ct value was 34, corresponding to 3.6 log10 DNA copies/mL. Results for the other samples were negative. Using morphologic criteria, we identified all 16 ticks collected from the sloth as Amblyomma geayi (Figure, panel B). We performed C. burnetii–specific qPCR on the ticks; 14 (88%) were positive. We genotyped C. burnetii–positive DNA from the feces and from 6 of the 16 ticks by using multispacer Sequence typing as described (5). All samples were identified as MST17, the unique genotype circulating in Cayenne (5). After obtaining the laboratory results, we confirmed that a local group in charge of the collection and treatment of injured animals usually released rehabilitated 3-toed sloths into Tiger Camp. Residents of Tiger Camp regularly observed and came into contact with the sloths, and ticks were frequently observed on the fur of the animals. Furthermore, 3 Q fever patients from Cayenne reported contact with sloths. Feces from the sloth in this study were highly infectious for C. burnetii. Because sloths live in tall trees and can shed this bacterium in their feces, human contamination might occur through inhalation of infectious aerosols from feces. The high prevalence of C. burnetii infection in ticks also suggests possible transmission through tick bites or from aerosols of tick feces that have been deposited on the skin of animal hosts; such feces can be extremely rich in bacteria and highly infectious (10). In this 2013 outbreak of Q fever, epidemiologic studies led to the identification of 3-toed sloths as a putative source of C. burnetii infection. Further investigations are needed to confirm the role of sloths as a reservoir for C. burnetii in French Guiana and to implement efficient measures to prevent transmission to humans.
Jean-lou Marié - One of the best experts on this subject based on the ideXlab platform.
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three toed sloth as putative reservoir of coxiella burnetii cayenne french guiana
Emerging Infectious Diseases, 2014Co-Authors: Bernard Davoust, Jean-lou Marié, Vincent Pommier De Santi, Jean Michel Berenger, Sophie Edouard, Didier RaoultAbstract:To the Editor: Q fever is an emerging zoonosis and a major public health concern in French Guiana, a French overseas region located on the northeastern coast of South America (1,2). Most cases occur in the city of Cayenne (3), specifically in the suburbs, where houses are near wooded hills (4). Genotyping performed by using multispacer Sequence typing showed that MST17, a unique genotype of C. burnetii, circulates in Cayenne and is responsible for epidemics of Q fever (5). C. burnetii transmission peaks during the rainy season, and the incidence of Q fever usually increases 1–3 months later (6). The animal reservoir of C. burnetii in French Guiana is unknown; previous studies have excluded domestic ruminants, which are known to be C. burnetii reservoirs elsewhere in the world (6). Four serologic surveys showed few C. burnetii–positive opossums, dogs, rodents (Proechimys spp.), bovines, or birds in French Guiana (7). In 2013, using real-time PCR (qPCR) analysis of vaginal swab samples, we showed that 6/158 (3.8%) dogs from Cayenne and 0/206 bats from the coastal area of French Guiana were positive for C. burnetii (cycle threshold [Ct]<35). One of the positive samples was identified as genotype MST17 (5). A case–control study among humans identified several risk factors for Q fever, including living near a forest and the presence of wild animals near the house (6). During January–April 2013, a Q fever outbreak occurred in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne. Vaginal swab samples were collected from animals living in the area (13 goats, 8 sheep, 7 bats, 34 birds, 2 opossums, 4 iguanas, and 17 geckos); all samples were negative for C. burnetii by qPCR. In addition, serologic tests for C. burnetii were negative for samples from all 37 small ruminants maintained near the outbreak area. In January 2014, a dead (accidental death) female 3-toed sloth (Bradypus tridactylus) (Figure, panel A) was found on the road near the residence of a Q fever patient. We retrieved the sloth and collected feces, spleen, liver, kidney, lung, and uterus samples and a vaginal swab sample. A total of 16 ticks were removed from the sloth and stored in 70% alcohol. Figure Putative reservoir of Coxiella burnetii in Cayenne, French Guiana A) A 3-toed sloth (Bradypus tridactylus) in its natural habitat in Tiger Camp, a military residential area located at the top of a wooded hill in Cayenne, French Guiana (photograph by S. ... DNA was extracted from the feces, organs, and ticks by using the BioRobot EZ1 Workstation (QIAGEN, Courtaboeuf, France). qPCR targeting the Repeated insertion Sequence IS1111 was performed by using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Marne la Coquette, France) as described (8). We confirmed all positive results by performing a second qPCR targeting the IS30a Repeated Sequence. DNA samples with Ct values <35 in both assays were considered positive for C. burnetii. A standard calibration curve quantifying the target IS1111 was generated by using 10-fold serial dilutions of C. burnetii Nine Mile strain. The number of IS1111 intergenic Sequences found in the genome of strain C. burnetii MST17 was identical to that for the Nine Mile strain (F. D’Amato, unpub. data); thus, the qPCR that we used was valid for quantifying the number of C. burnetii MST17 IS1111 copies/mL in samples we collected (5). qPCR analysis showed that the feces were highly positive for C. burnetii; the sample had a low Ct value of 23, corresponding to 7 log10 DNA copies/mL (9). The spleen was also positive for C. burnetii; the Ct value was 34, corresponding to 3.6 log10 DNA copies/mL. Results for the other samples were negative. Using morphologic criteria, we identified all 16 ticks collected from the sloth as Amblyomma geayi (Figure, panel B). We performed C. burnetii–specific qPCR on the ticks; 14 (88%) were positive. We genotyped C. burnetii–positive DNA from the feces and from 6 of the 16 ticks by using multispacer Sequence typing as described (5). All samples were identified as MST17, the unique genotype circulating in Cayenne (5). After obtaining the laboratory results, we confirmed that a local group in charge of the collection and treatment of injured animals usually released rehabilitated 3-toed sloths into Tiger Camp. Residents of Tiger Camp regularly observed and came into contact with the sloths, and ticks were frequently observed on the fur of the animals. Furthermore, 3 Q fever patients from Cayenne reported contact with sloths. Feces from the sloth in this study were highly infectious for C. burnetii. Because sloths live in tall trees and can shed this bacterium in their feces, human contamination might occur through inhalation of infectious aerosols from feces. The high prevalence of C. burnetii infection in ticks also suggests possible transmission through tick bites or from aerosols of tick feces that have been deposited on the skin of animal hosts; such feces can be extremely rich in bacteria and highly infectious (10). In this 2013 outbreak of Q fever, epidemiologic studies led to the identification of 3-toed sloths as a putative source of C. burnetii infection. Further investigations are needed to confirm the role of sloths as a reservoir for C. burnetii in French Guiana and to implement efficient measures to prevent transmission to humans.