The Experts below are selected from a list of 1377 Experts worldwide ranked by ideXlab platform
Javier Marcos - One of the best experts on this subject based on the ideXlab platform.
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Class 1 integrons in Acinetobacter baumannii: a weak expression of gene cassettes to counterbalance the lack of lexA-driven integrase repression
International Journal of Antimicrobial Agents, 2018Co-Authors: Elodie Couvé-deacon, Olivier Barraud, Thomas Jové, Pamela Afouda, Valentin Tilloy, Erwan Scaon, Bastien Hervé, Christophe Burucoa, Marie Kempf, Javier MarcosAbstract:Integrons are able to recruit resistance genes through integrase-driven recombination events that are regulated by the bacterial SOS response and require the Repressor lexA. Class 1 integrons genes are expressed from a common promoter, Pc, of which at least 5 predominant variants, classified from weak to strong, have been described. In Escherichia coli, there is an intertwined regulation between gene cassette expression and integrase activity: the stronger the promoter is, the weaker the integrase is. Class 1 integrons have been frequently described in Acinetobacter baumannii. However, Acinetobacter spp. lack the lexA Repressor suggesting that the integrase is constitutively expressed. We characterized the integron content of 83 clinical and environmental A. baumannii strains. We found a predominance of Pc variants described as strong in E. coli. The Pc expression level was 2 to 4-fold lower in A. baumannii than in E. coli, and the diversity of the gene cassette array was low. In A.baumannii integrons with a PcS promoter might have been selected to allow a sufficient resistance level while avoiding the toxicity of a highly active integrase. Furthermore, a transcriptional interference between PcS and PintI1 (as shown in E.coli) may limit the expression of the integrase and thus counterbalance the lack of lexA-driven integrase repression to prevent the cost of the integrase.
Elodie Couvé-deacon - One of the best experts on this subject based on the ideXlab platform.
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Class 1 integrons in Acinetobacter baumannii: a weak expression of gene cassettes to counterbalance the lack of lexA-driven integrase repression
International Journal of Antimicrobial Agents, 2018Co-Authors: Elodie Couvé-deacon, Olivier Barraud, Thomas Jové, Pamela Afouda, Valentin Tilloy, Erwan Scaon, Bastien Hervé, Christophe Burucoa, Marie Kempf, Javier MarcosAbstract:Integrons are able to recruit resistance genes through integrase-driven recombination events that are regulated by the bacterial SOS response and require the Repressor lexA. Class 1 integrons genes are expressed from a common promoter, Pc, of which at least 5 predominant variants, classified from weak to strong, have been described. In Escherichia coli, there is an intertwined regulation between gene cassette expression and integrase activity: the stronger the promoter is, the weaker the integrase is. Class 1 integrons have been frequently described in Acinetobacter baumannii. However, Acinetobacter spp. lack the lexA Repressor suggesting that the integrase is constitutively expressed. We characterized the integron content of 83 clinical and environmental A. baumannii strains. We found a predominance of Pc variants described as strong in E. coli. The Pc expression level was 2 to 4-fold lower in A. baumannii than in E. coli, and the diversity of the gene cassette array was low. In A.baumannii integrons with a PcS promoter might have been selected to allow a sufficient resistance level while avoiding the toxicity of a highly active integrase. Furthermore, a transcriptional interference between PcS and PintI1 (as shown in E.coli) may limit the expression of the integrase and thus counterbalance the lack of lexA-driven integrase repression to prevent the cost of the integrase.
Olivier Barraud - One of the best experts on this subject based on the ideXlab platform.
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Class 1 integrons in Acinetobacter baumannii: a weak expression of gene cassettes to counterbalance the lack of lexA-driven integrase repression
International Journal of Antimicrobial Agents, 2018Co-Authors: Elodie Couvé-deacon, Olivier Barraud, Thomas Jové, Pamela Afouda, Valentin Tilloy, Erwan Scaon, Bastien Hervé, Christophe Burucoa, Marie Kempf, Javier MarcosAbstract:Integrons are able to recruit resistance genes through integrase-driven recombination events that are regulated by the bacterial SOS response and require the Repressor lexA. Class 1 integrons genes are expressed from a common promoter, Pc, of which at least 5 predominant variants, classified from weak to strong, have been described. In Escherichia coli, there is an intertwined regulation between gene cassette expression and integrase activity: the stronger the promoter is, the weaker the integrase is. Class 1 integrons have been frequently described in Acinetobacter baumannii. However, Acinetobacter spp. lack the lexA Repressor suggesting that the integrase is constitutively expressed. We characterized the integron content of 83 clinical and environmental A. baumannii strains. We found a predominance of Pc variants described as strong in E. coli. The Pc expression level was 2 to 4-fold lower in A. baumannii than in E. coli, and the diversity of the gene cassette array was low. In A.baumannii integrons with a PcS promoter might have been selected to allow a sufficient resistance level while avoiding the toxicity of a highly active integrase. Furthermore, a transcriptional interference between PcS and PintI1 (as shown in E.coli) may limit the expression of the integrase and thus counterbalance the lack of lexA-driven integrase repression to prevent the cost of the integrase.
Christophe Burucoa - One of the best experts on this subject based on the ideXlab platform.
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Class 1 integrons in Acinetobacter baumannii: a weak expression of gene cassettes to counterbalance the lack of lexA-driven integrase repression
International Journal of Antimicrobial Agents, 2018Co-Authors: Elodie Couvé-deacon, Olivier Barraud, Thomas Jové, Pamela Afouda, Valentin Tilloy, Erwan Scaon, Bastien Hervé, Christophe Burucoa, Marie Kempf, Javier MarcosAbstract:Integrons are able to recruit resistance genes through integrase-driven recombination events that are regulated by the bacterial SOS response and require the Repressor lexA. Class 1 integrons genes are expressed from a common promoter, Pc, of which at least 5 predominant variants, classified from weak to strong, have been described. In Escherichia coli, there is an intertwined regulation between gene cassette expression and integrase activity: the stronger the promoter is, the weaker the integrase is. Class 1 integrons have been frequently described in Acinetobacter baumannii. However, Acinetobacter spp. lack the lexA Repressor suggesting that the integrase is constitutively expressed. We characterized the integron content of 83 clinical and environmental A. baumannii strains. We found a predominance of Pc variants described as strong in E. coli. The Pc expression level was 2 to 4-fold lower in A. baumannii than in E. coli, and the diversity of the gene cassette array was low. In A.baumannii integrons with a PcS promoter might have been selected to allow a sufficient resistance level while avoiding the toxicity of a highly active integrase. Furthermore, a transcriptional interference between PcS and PintI1 (as shown in E.coli) may limit the expression of the integrase and thus counterbalance the lack of lexA-driven integrase repression to prevent the cost of the integrase.
Thomas Jové - One of the best experts on this subject based on the ideXlab platform.
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Class 1 integrons in Acinetobacter baumannii: a weak expression of gene cassettes to counterbalance the lack of lexA-driven integrase repression
International Journal of Antimicrobial Agents, 2018Co-Authors: Elodie Couvé-deacon, Olivier Barraud, Thomas Jové, Pamela Afouda, Valentin Tilloy, Erwan Scaon, Bastien Hervé, Christophe Burucoa, Marie Kempf, Javier MarcosAbstract:Integrons are able to recruit resistance genes through integrase-driven recombination events that are regulated by the bacterial SOS response and require the Repressor lexA. Class 1 integrons genes are expressed from a common promoter, Pc, of which at least 5 predominant variants, classified from weak to strong, have been described. In Escherichia coli, there is an intertwined regulation between gene cassette expression and integrase activity: the stronger the promoter is, the weaker the integrase is. Class 1 integrons have been frequently described in Acinetobacter baumannii. However, Acinetobacter spp. lack the lexA Repressor suggesting that the integrase is constitutively expressed. We characterized the integron content of 83 clinical and environmental A. baumannii strains. We found a predominance of Pc variants described as strong in E. coli. The Pc expression level was 2 to 4-fold lower in A. baumannii than in E. coli, and the diversity of the gene cassette array was low. In A.baumannii integrons with a PcS promoter might have been selected to allow a sufficient resistance level while avoiding the toxicity of a highly active integrase. Furthermore, a transcriptional interference between PcS and PintI1 (as shown in E.coli) may limit the expression of the integrase and thus counterbalance the lack of lexA-driven integrase repression to prevent the cost of the integrase.