The Experts below are selected from a list of 22119 Experts worldwide ranked by ideXlab platform
Gregor Weiss - One of the best experts on this subject based on the ideXlab platform.
-
endometriosis accelerates synchronization of early embryo cell divisions but does not change morphokinetic dynamics in endometriosis patients
PLOS ONE, 2019Co-Authors: Michael Schenk, Julia M Kropfl, Martina Hormannkropfl, Gregor WeissAbstract:Objective The pathology of endometriosis and its impact on embryo development is still a black box in Reproductive Medicine. In this time-lapse study we investigated the influence of endometriosis on morphokinetic parameters of embryo development, taking variables of dynamic monitoring into account. Furthermore we evaluated Reproductive Medicine treatment outcome such as fetal heartbeat and live birth rate. Methods 1148 embryos (control: n = 596, endometriosis: n = 552) were retrospectively analyzed. Patients were stimulated with GnRH antagonist protocol. After fertilization, embryos were incubated in a time-lapse system (EmbryoScope). Results The mixed-model analysis revealed a significant main effect of time (p<0.001), with post-hoc tests showing that any time needed to reach a specific developmental stage was significantly different from all the others (all p<0.001). Embryos of endometriosis patients showed the same absolute morphokinetic time parameters as the control group, however, synchronization of early embryo cell divisions (s2) was faster in endometriosis patients compared to the control group. Conclusion In general, endometriosis does not induce changes in early embryo morphokinetics. However, observed acceleration in cell cycle synchronization of embryo cleavage patterns might be a missing explanation for contradicting results in literature regarding the impairments in Reproductive Medicine treatment outcome of endometriosis patients.
Sylvie Dubanchet - One of the best experts on this subject based on the ideXlab platform.
-
the uterine immune profile may help women with repeated unexplained embryo implantation failure after in vitro fertilization
American Journal of Reproductive Immunology, 2016Co-Authors: Nathalie Ledee, Marie Petitbarat, Lucie Chevrier, Dominique Vitoux, Katia Vezmar, Mona Rahmati, Sylvie DubanchetAbstract:Labeled problem Embryo implantation remains the main limiting factor in assisted Reproductive Medicine (20% success rate).
David B Seifer - One of the best experts on this subject based on the ideXlab platform.
-
why we may abandon basal follicle stimulating hormone testing a sea change in determining ovarian reserve using antimullerian hormone
Fertility and Sterility, 2013Co-Authors: James P Toner, David B SeiferAbstract:Antimullerian hormone is the most informative serum marker of ovarian reserve currently available and should be considered an important part of any contemporary Reproductive Medicine practice. It is both more convenient and informative than basal FSH and can be assessed at any point in the cycle. It is the most useful serum method of determining ovarian reserve, which guides pretreatment counseling, choice of infertility treatment, and avoidance of ovarian hyperstimulation. The future role of basal FSH testing is in doubt.
Michael Schenk - One of the best experts on this subject based on the ideXlab platform.
-
endometriosis accelerates synchronization of early embryo cell divisions but does not change morphokinetic dynamics in endometriosis patients
PLOS ONE, 2019Co-Authors: Michael Schenk, Julia M Kropfl, Martina Hormannkropfl, Gregor WeissAbstract:Objective The pathology of endometriosis and its impact on embryo development is still a black box in Reproductive Medicine. In this time-lapse study we investigated the influence of endometriosis on morphokinetic parameters of embryo development, taking variables of dynamic monitoring into account. Furthermore we evaluated Reproductive Medicine treatment outcome such as fetal heartbeat and live birth rate. Methods 1148 embryos (control: n = 596, endometriosis: n = 552) were retrospectively analyzed. Patients were stimulated with GnRH antagonist protocol. After fertilization, embryos were incubated in a time-lapse system (EmbryoScope). Results The mixed-model analysis revealed a significant main effect of time (p<0.001), with post-hoc tests showing that any time needed to reach a specific developmental stage was significantly different from all the others (all p<0.001). Embryos of endometriosis patients showed the same absolute morphokinetic time parameters as the control group, however, synchronization of early embryo cell divisions (s2) was faster in endometriosis patients compared to the control group. Conclusion In general, endometriosis does not induce changes in early embryo morphokinetics. However, observed acceleration in cell cycle synchronization of embryo cleavage patterns might be a missing explanation for contradicting results in literature regarding the impairments in Reproductive Medicine treatment outcome of endometriosis patients.
Scott J. Paynter - One of the best experts on this subject based on the ideXlab platform.
-
fundamentals of cryobiology in Reproductive Medicine
Reproductive Biomedicine Online, 2004Co-Authors: Barry Fuller, Scott J. PaynterAbstract:Abstract The aim of this review will be to provide a basic understanding of the biophysical processes that accompany the application of cryopreservation in Reproductive Medicine. The ability to store cells in ‘suspended animation' outside the body has become a keystone practice in the development of many modern clinical therapies, and, in fact, the sciences of cryobiology and IVF have developed in parallel over the past 50 years. During this time, some of the underlying principles of the quantitative biophysical aspects of cryobiology have been clarified. Water is the universal biocompatible solvent, but also possesses unique properties for stability of living cells. Whilst low temperatures themselves have defined effects on cell structure and function, it is the phase transition of water to ice that is the most profound challenge for survival. The thermodynamics of dilute aqueous solutions dictate how cells and tissues respond to the freezing process. Current concepts of nucleation, ice crystal growth and solute exclusion from the ice lattice will be discussed to illustrate what cells must negotiate to avoid lethal damage, and the role of cryoprotectants in enhancing recovery. Quantitative formalisms now exist to model and predict how water and solutes move across cell membranes before and during freezing, or how nucleation events will proceed, and these will be outlined. Cryoprotectants have both positive and negative effects on cell function depending on the kinetics of exposure. The concept of tolerable osmotic excursion of cell volume will be discussed, along with the evidence for a ‘pseudo-glassy' state for cells during traditional cryopreservation. This will be compared with the recent interest in promoting glassy states in the whole sample using vitrification protocols, outlining the advantages and drawbacks of each approach. Additional methods for controlling ice nucleation have a role to play here, and a brief outline of current technologies will be given. Finally, issues of safety and stability of cryopreserved samples will be set out.
