The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Wen Chieh Tsai - One of the best experts on this subject based on the ideXlab platform.
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the ancestral duplicated dl crc orthologs pedl1 and pedl2 function in orchid Reproductive Organ innovation
Journal of Experimental Botany, 2021Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:Orchid gynostemium, the fused Organ of the androecium and gynoecium, and ovule development are unique developmental processes. Two DROOPING LEAF/CRABS CLAW (DL/CRC) genes, PeDL1 and PeDL2, were identified from the Phalaenopsis orchid genome and functionally characterized. Phylogenetic analysis indicated that the most recent common ancestor of orchids contained the duplicated DL/CRC-like genes. Temporal and spatial expression analysis indicated that PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Virus-induced gene silencing (VIGS) of PeDL1 and PeDL2 affected the number of protuberant ovule initials differentiated from the placenta. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, but not PeDL2, could form a heterodimer with Phalaenopsis equestris CINCINNATA 8 (PeCIN8). Paralogous retention and subsequent divergence of the gene sequences of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal that the ancestral duplicated DL/CRC-like genes play important roles in orchid Reproductive Organ innovation.
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ancestral duplicated dl crc orthologs display function on orchid Reproductive Organ innovation
bioRxiv, 2020Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:ABSTRACT The orchid flower is renowned for complexity of flower Organ morphogenesis and has attracted great interest from scientists. The YABBY genes encode plant-specific transcription factors with important roles in vegetative and Reproductive development in seed plants. DROOPING LEAF/CRABS CLAW (DL/CRC) orthologs are involved in Reproductive Organ development (especially carpels) of angiosperms. Orchid gynostemium (the fused Organ of the androecium and gynoecium) and ovule development are unique developmental processes. Understanding the DL/CRC-like genes controlling the developmental program of the gynostemium and ovule could provide accessible information for Reproductive Organ molecular regulation in orchids. Two DL/CRC-like genes, named PeDL1 and PeDL2, were cloned from Phalaenopsis equestris. The orchid DL/CRC forms a monophyletic clade with two subclades including AshDL, PeDL1 and DcaDL1 in subclade I, and PeDL2 and DcaDL2 in subclade II. The temporal and spatial expression analysis indicated PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, instead of PeDL2, could form a heterodimer with PeCIN8. Paralogue retention and subsequent divergence of the gene sequence of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal the important roles of PeDLs involved in orchid gynostemium and ovule development and provide new insights for further understanding the molecular mechanisms underlying orchid Reproductive Organ development.
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duplicated c class mads box genes reveal distinct roles in gynostemium development in cymbidium ensifolium orchidaceae
Plant and Cell Physiology, 2011Co-Authors: Shih Yu Wang, You Yi Chen, Yu Yun Hsiao, Zhongjian Liu, Pei Fang Lee, Yung I Lee, Zhao Jun Pan, Wen Chieh TsaiAbstract:The orchid floral Organs represent novel and effective structures for attracting pollination vectors. In addition, to avoid inbreeding, the androecium and gynoecium are united in a single structure termed the gynostemium. Identification of C-class MADS-box genes regulating Reproductive Organ development could help determine the level of homology with the current ABC model of floral Organ identity in orchids. In this study, we isolated and characterized two C-class AGAMOUS-like genes, denoted CeMADS1 and CeMADS2, from Cymbidium ensifolium. These two genes showed distinct spatial and temporal expression profiles, which suggests their functional diversification during gynostemium development. Furthermore, the expression of CeMADS1 but not CeMADS2 was eliminated in the multitepal mutant whose gynostemium is replaced by a newly emerged flower, and this ecotopic flower continues to produce sepals and petals centripetally. Protein interaction relationships among CeMADS1, CeMADS2 and E-class PeMADS8 proteins were assessed by yeast two-hybrid analysis. Both CeMADS1 and CeMADS2 formed homodimers and heterodimers with each other and the E-class PeMADS protein. Furthermore, transgenic Arabidopsis plants overexpressing CeMADS1 or CeMADS2 showed limited growth of primary inflorescence. Thus, CeMADS1 may have a pivotal C function in Reproductive Organ development in C. ensifolium.
You Yi Chen - One of the best experts on this subject based on the ideXlab platform.
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the ancestral duplicated dl crc orthologs pedl1 and pedl2 function in orchid Reproductive Organ innovation
Journal of Experimental Botany, 2021Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:Orchid gynostemium, the fused Organ of the androecium and gynoecium, and ovule development are unique developmental processes. Two DROOPING LEAF/CRABS CLAW (DL/CRC) genes, PeDL1 and PeDL2, were identified from the Phalaenopsis orchid genome and functionally characterized. Phylogenetic analysis indicated that the most recent common ancestor of orchids contained the duplicated DL/CRC-like genes. Temporal and spatial expression analysis indicated that PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Virus-induced gene silencing (VIGS) of PeDL1 and PeDL2 affected the number of protuberant ovule initials differentiated from the placenta. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, but not PeDL2, could form a heterodimer with Phalaenopsis equestris CINCINNATA 8 (PeCIN8). Paralogous retention and subsequent divergence of the gene sequences of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal that the ancestral duplicated DL/CRC-like genes play important roles in orchid Reproductive Organ innovation.
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ancestral duplicated dl crc orthologs display function on orchid Reproductive Organ innovation
bioRxiv, 2020Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:ABSTRACT The orchid flower is renowned for complexity of flower Organ morphogenesis and has attracted great interest from scientists. The YABBY genes encode plant-specific transcription factors with important roles in vegetative and Reproductive development in seed plants. DROOPING LEAF/CRABS CLAW (DL/CRC) orthologs are involved in Reproductive Organ development (especially carpels) of angiosperms. Orchid gynostemium (the fused Organ of the androecium and gynoecium) and ovule development are unique developmental processes. Understanding the DL/CRC-like genes controlling the developmental program of the gynostemium and ovule could provide accessible information for Reproductive Organ molecular regulation in orchids. Two DL/CRC-like genes, named PeDL1 and PeDL2, were cloned from Phalaenopsis equestris. The orchid DL/CRC forms a monophyletic clade with two subclades including AshDL, PeDL1 and DcaDL1 in subclade I, and PeDL2 and DcaDL2 in subclade II. The temporal and spatial expression analysis indicated PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, instead of PeDL2, could form a heterodimer with PeCIN8. Paralogue retention and subsequent divergence of the gene sequence of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal the important roles of PeDLs involved in orchid gynostemium and ovule development and provide new insights for further understanding the molecular mechanisms underlying orchid Reproductive Organ development.
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duplicated c class mads box genes reveal distinct roles in gynostemium development in cymbidium ensifolium orchidaceae
Plant and Cell Physiology, 2011Co-Authors: Shih Yu Wang, You Yi Chen, Yu Yun Hsiao, Zhongjian Liu, Pei Fang Lee, Yung I Lee, Zhao Jun Pan, Wen Chieh TsaiAbstract:The orchid floral Organs represent novel and effective structures for attracting pollination vectors. In addition, to avoid inbreeding, the androecium and gynoecium are united in a single structure termed the gynostemium. Identification of C-class MADS-box genes regulating Reproductive Organ development could help determine the level of homology with the current ABC model of floral Organ identity in orchids. In this study, we isolated and characterized two C-class AGAMOUS-like genes, denoted CeMADS1 and CeMADS2, from Cymbidium ensifolium. These two genes showed distinct spatial and temporal expression profiles, which suggests their functional diversification during gynostemium development. Furthermore, the expression of CeMADS1 but not CeMADS2 was eliminated in the multitepal mutant whose gynostemium is replaced by a newly emerged flower, and this ecotopic flower continues to produce sepals and petals centripetally. Protein interaction relationships among CeMADS1, CeMADS2 and E-class PeMADS8 proteins were assessed by yeast two-hybrid analysis. Both CeMADS1 and CeMADS2 formed homodimers and heterodimers with each other and the E-class PeMADS protein. Furthermore, transgenic Arabidopsis plants overexpressing CeMADS1 or CeMADS2 showed limited growth of primary inflorescence. Thus, CeMADS1 may have a pivotal C function in Reproductive Organ development in C. ensifolium.
Zhongjian Liu - One of the best experts on this subject based on the ideXlab platform.
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the ancestral duplicated dl crc orthologs pedl1 and pedl2 function in orchid Reproductive Organ innovation
Journal of Experimental Botany, 2021Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:Orchid gynostemium, the fused Organ of the androecium and gynoecium, and ovule development are unique developmental processes. Two DROOPING LEAF/CRABS CLAW (DL/CRC) genes, PeDL1 and PeDL2, were identified from the Phalaenopsis orchid genome and functionally characterized. Phylogenetic analysis indicated that the most recent common ancestor of orchids contained the duplicated DL/CRC-like genes. Temporal and spatial expression analysis indicated that PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Virus-induced gene silencing (VIGS) of PeDL1 and PeDL2 affected the number of protuberant ovule initials differentiated from the placenta. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, but not PeDL2, could form a heterodimer with Phalaenopsis equestris CINCINNATA 8 (PeCIN8). Paralogous retention and subsequent divergence of the gene sequences of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal that the ancestral duplicated DL/CRC-like genes play important roles in orchid Reproductive Organ innovation.
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ancestral duplicated dl crc orthologs display function on orchid Reproductive Organ innovation
bioRxiv, 2020Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:ABSTRACT The orchid flower is renowned for complexity of flower Organ morphogenesis and has attracted great interest from scientists. The YABBY genes encode plant-specific transcription factors with important roles in vegetative and Reproductive development in seed plants. DROOPING LEAF/CRABS CLAW (DL/CRC) orthologs are involved in Reproductive Organ development (especially carpels) of angiosperms. Orchid gynostemium (the fused Organ of the androecium and gynoecium) and ovule development are unique developmental processes. Understanding the DL/CRC-like genes controlling the developmental program of the gynostemium and ovule could provide accessible information for Reproductive Organ molecular regulation in orchids. Two DL/CRC-like genes, named PeDL1 and PeDL2, were cloned from Phalaenopsis equestris. The orchid DL/CRC forms a monophyletic clade with two subclades including AshDL, PeDL1 and DcaDL1 in subclade I, and PeDL2 and DcaDL2 in subclade II. The temporal and spatial expression analysis indicated PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, instead of PeDL2, could form a heterodimer with PeCIN8. Paralogue retention and subsequent divergence of the gene sequence of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal the important roles of PeDLs involved in orchid gynostemium and ovule development and provide new insights for further understanding the molecular mechanisms underlying orchid Reproductive Organ development.
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duplicated c class mads box genes reveal distinct roles in gynostemium development in cymbidium ensifolium orchidaceae
Plant and Cell Physiology, 2011Co-Authors: Shih Yu Wang, You Yi Chen, Yu Yun Hsiao, Zhongjian Liu, Pei Fang Lee, Yung I Lee, Zhao Jun Pan, Wen Chieh TsaiAbstract:The orchid floral Organs represent novel and effective structures for attracting pollination vectors. In addition, to avoid inbreeding, the androecium and gynoecium are united in a single structure termed the gynostemium. Identification of C-class MADS-box genes regulating Reproductive Organ development could help determine the level of homology with the current ABC model of floral Organ identity in orchids. In this study, we isolated and characterized two C-class AGAMOUS-like genes, denoted CeMADS1 and CeMADS2, from Cymbidium ensifolium. These two genes showed distinct spatial and temporal expression profiles, which suggests their functional diversification during gynostemium development. Furthermore, the expression of CeMADS1 but not CeMADS2 was eliminated in the multitepal mutant whose gynostemium is replaced by a newly emerged flower, and this ecotopic flower continues to produce sepals and petals centripetally. Protein interaction relationships among CeMADS1, CeMADS2 and E-class PeMADS8 proteins were assessed by yeast two-hybrid analysis. Both CeMADS1 and CeMADS2 formed homodimers and heterodimers with each other and the E-class PeMADS protein. Furthermore, transgenic Arabidopsis plants overexpressing CeMADS1 or CeMADS2 showed limited growth of primary inflorescence. Thus, CeMADS1 may have a pivotal C function in Reproductive Organ development in C. ensifolium.
Chuan Ming Yeh - One of the best experts on this subject based on the ideXlab platform.
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the ancestral duplicated dl crc orthologs pedl1 and pedl2 function in orchid Reproductive Organ innovation
Journal of Experimental Botany, 2021Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:Orchid gynostemium, the fused Organ of the androecium and gynoecium, and ovule development are unique developmental processes. Two DROOPING LEAF/CRABS CLAW (DL/CRC) genes, PeDL1 and PeDL2, were identified from the Phalaenopsis orchid genome and functionally characterized. Phylogenetic analysis indicated that the most recent common ancestor of orchids contained the duplicated DL/CRC-like genes. Temporal and spatial expression analysis indicated that PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Virus-induced gene silencing (VIGS) of PeDL1 and PeDL2 affected the number of protuberant ovule initials differentiated from the placenta. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, but not PeDL2, could form a heterodimer with Phalaenopsis equestris CINCINNATA 8 (PeCIN8). Paralogous retention and subsequent divergence of the gene sequences of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal that the ancestral duplicated DL/CRC-like genes play important roles in orchid Reproductive Organ innovation.
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ancestral duplicated dl crc orthologs display function on orchid Reproductive Organ innovation
bioRxiv, 2020Co-Authors: You Yi Chen, Yu Yun Hsiao, Chuan Ming Yeh, Nobutaka Mitsuda, Hongxing Yang, Chi Chou Chiu, Song Bin Chang, Zhongjian Liu, Wen Chieh TsaiAbstract:ABSTRACT The orchid flower is renowned for complexity of flower Organ morphogenesis and has attracted great interest from scientists. The YABBY genes encode plant-specific transcription factors with important roles in vegetative and Reproductive development in seed plants. DROOPING LEAF/CRABS CLAW (DL/CRC) orthologs are involved in Reproductive Organ development (especially carpels) of angiosperms. Orchid gynostemium (the fused Organ of the androecium and gynoecium) and ovule development are unique developmental processes. Understanding the DL/CRC-like genes controlling the developmental program of the gynostemium and ovule could provide accessible information for Reproductive Organ molecular regulation in orchids. Two DL/CRC-like genes, named PeDL1 and PeDL2, were cloned from Phalaenopsis equestris. The orchid DL/CRC forms a monophyletic clade with two subclades including AshDL, PeDL1 and DcaDL1 in subclade I, and PeDL2 and DcaDL2 in subclade II. The temporal and spatial expression analysis indicated PeDL genes are specifically expressed in the gynostemium and at the early stages of ovule development. Both PeDLs could partially complement an Arabidopsis crc-1 mutant. Transient overexpression of PeDL1 in Phalaenopsis orchids caused abnormal development of ovule and stigmatic cavity of gynostemium. PeDL1, instead of PeDL2, could form a heterodimer with PeCIN8. Paralogue retention and subsequent divergence of the gene sequence of PeDL1 and PeDL2 in P. equestris might result in the differentiation of function and protein behaviors. These results reveal the important roles of PeDLs involved in orchid gynostemium and ovule development and provide new insights for further understanding the molecular mechanisms underlying orchid Reproductive Organ development.
Nathan I Morehouse - One of the best experts on this subject based on the ideXlab platform.
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a novel female Reproductive Organ the bursa copulatrix as a dynamic interface for male female coevolution
Annual Meeting Society for Molecular Biology and Evolution (SMBE), 2017Co-Authors: Camille Meslin, Melissa S Plakke, Nathan I Morehouse, Brandon S Small, Tamara S Cherwin, Nathan L ClarkAbstract:A novel female Reproductive Organ - the bursa copulatrix - as a dynamic interface for male-female coevolution. Annual Meeting Society for Molecular Biology and Evolution (SMBE)
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dynamic digestive physiology of a female Reproductive Organ in a polyandrous butterfly
Evolution, 2015Co-Authors: Melissa S Plakke, Aaron B Deutsch, Camille Meslin, Nathan L Clark, Nathan I MorehouseAbstract:Dynamic digestive physiology of a female Reproductive Organ in a polyandrous butterfly. Evolution
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Dynamic digestive physiology of a female Reproductive Organ in a polyandrous butterfly.
Journal of Experimental Biology, 2015Co-Authors: Melissa S Plakke, Aaron B Deutsch, Camille Meslin, Nathan L Clark, Nathan I MorehouseAbstract:Reproductive traits experience high levels of selection because of their direct ties to fitness, often resulting in rapid adaptive evolution. Much of the work in this area has focused on male Reproductive traits. However, a more comprehensive understanding of female Reproductive adaptations and their relationship to male characters is crucial to uncover the relative roles of sexual cooperation and conflict in driving co-evolutionary dynamics between the sexes. We focus on the physiology of a complex female Reproductive adaptation in butterflies and moths: a stomach-like Organ in the female Reproductive tract called the bursa copulatrix that digests the male ejaculate (spermatophore). Little is known about how the bursa digests the spermatophore. We characterized bursa proteolytic capacity in relation to female state in the polyandrous butterfly Pieris rapae. We found that the virgin bursa exhibits extremely high levels of proteolytic activity. Furthermore, in virgin females, bursal proteolytic capacity increases with time since eclosion and ambient temperature, but is not sensitive to the pre-mating social environment. Post copulation, bursal proteolytic activity decreases rapidly before rebounding toward the end of a mating cycle, suggesting active female regulation of proteolysis and/or potential quenching of proteolysis by male ejaculate constituents. Using transcriptomic and proteomic approaches, we report identities for nine proteases actively transcribed by bursal tissue and/or expressed in the bursal lumen that may contribute to observed bursal proteolysis. We discuss how these dynamic physiological characteristics may function as female adaptations resulting from sexual conflict over female remating rate in this polyandrous butterfly.
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extreme digestive physiology in a female Reproductive Organ mediating sexual conflict in a polyandrous butterfly
International Society for Behavioral Ecology ISBE, 2014Co-Authors: Melissa S Plakke, Aaron B Deutsch, Camille Meslin, Nathan L Clark, Nathan I MorehouseAbstract:Extreme digestive physiology in a female Reproductive Organ mediating sexual conflict in a polyandrous butterfly. International Society for Behavioral Ecology ISBE
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Molecular and evolutionary characterization of a unique Reproductive Organ in Lepidoptera, the bursa copulatrix
2014Co-Authors: Camille Meslin, Melissa S Plakke, Aaron B Deutsch, Nathan I Morehouse, Brandon S Small, Nathan L ClarkAbstract:Molecular and evolutionary characterization of a unique Reproductive Organ in Lepidoptera, the bursa copulatrix. Molecular Evolution Lab Discussion
