The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Magda Aparecida Beneventi - One of the best experts on this subject based on the ideXlab platform.
-
transcription profile of soybean root knot nematode interaction reveals a key role of phythormones in the Resistance Reaction
BMC Genomics, 2013Co-Authors: Magda Aparecida Beneventi, Orzenil Bonfim Da Silva, Alexandre Augusto Pereira Firmino, Regina Maria Santos De Amorim, Erika V S Albuquerque, Maria Cristina Mattar Da Silva, Joseane Padilha Da Silva, Magnolia De Araujo CamposAbstract:Root-knot nematodes (RKN– Meloidogyne genus) present extensive challenges to soybean crop. The soybean line (PI 595099) is known to be resistant against specific strains and races of nematode species, thus its differential gene expression analysis can lead to a comprehensive gene expression profiling in the incompatible soybean-RKN interaction. Even though many disease Resistance genes have been studied, little has been reported about phytohormone crosstalk on modulation of ROS signaling during soybean-RKN interaction. Using 454 technology to explore the common aspects of Resistance Reaction during both parasitism and Resistance phases it was verified that hormone, carbohydrate metabolism and stress related genes were consistently expressed at high levels in infected roots as compared to mock control. Most noteworthy genes include those encoding glycosyltransferases, peroxidases, auxin-responsive proteins and gibberellin-regulated genes. Our data analysis suggests the key role of glycosyltransferases, auxins and components of gibberellin signal transduction, biosynthesis and deactivation pathways in the Resistance Reaction and their participation in jasmonate signaling and redox homeostasis in mediating aspects of plant growth and responses to biotic stress. Based on this study we suggest a reasonable model regarding to the complex mechanisms of crosstalk between plant hormones, mainly gibberellins and auxins, which can be crucial to modulate the levels of ROS in the Resistance Reaction to nematode invasion. The model also includes recent findings concerning to the participation of DELLA-like proteins and ROS signaling controlling plant immune or stress responses. Furthermore, this study provides a dataset of potential candidate genes involved in both nematode parasitism and Resistance, which can be tested further for their role in this biological process using functional genomics approaches.
Magnolia De Araujo Campos - One of the best experts on this subject based on the ideXlab platform.
-
transcription profile of soybean root knot nematode interaction reveals a key role of phythormones in the Resistance Reaction
BMC Genomics, 2013Co-Authors: Magda Aparecida Beneventi, Orzenil Bonfim Da Silva, Alexandre Augusto Pereira Firmino, Regina Maria Santos De Amorim, Erika V S Albuquerque, Maria Cristina Mattar Da Silva, Joseane Padilha Da Silva, Magnolia De Araujo CamposAbstract:Root-knot nematodes (RKN– Meloidogyne genus) present extensive challenges to soybean crop. The soybean line (PI 595099) is known to be resistant against specific strains and races of nematode species, thus its differential gene expression analysis can lead to a comprehensive gene expression profiling in the incompatible soybean-RKN interaction. Even though many disease Resistance genes have been studied, little has been reported about phytohormone crosstalk on modulation of ROS signaling during soybean-RKN interaction. Using 454 technology to explore the common aspects of Resistance Reaction during both parasitism and Resistance phases it was verified that hormone, carbohydrate metabolism and stress related genes were consistently expressed at high levels in infected roots as compared to mock control. Most noteworthy genes include those encoding glycosyltransferases, peroxidases, auxin-responsive proteins and gibberellin-regulated genes. Our data analysis suggests the key role of glycosyltransferases, auxins and components of gibberellin signal transduction, biosynthesis and deactivation pathways in the Resistance Reaction and their participation in jasmonate signaling and redox homeostasis in mediating aspects of plant growth and responses to biotic stress. Based on this study we suggest a reasonable model regarding to the complex mechanisms of crosstalk between plant hormones, mainly gibberellins and auxins, which can be crucial to modulate the levels of ROS in the Resistance Reaction to nematode invasion. The model also includes recent findings concerning to the participation of DELLA-like proteins and ROS signaling controlling plant immune or stress responses. Furthermore, this study provides a dataset of potential candidate genes involved in both nematode parasitism and Resistance, which can be tested further for their role in this biological process using functional genomics approaches.
Alexandre Augusto Pereira Firmino - One of the best experts on this subject based on the ideXlab platform.
-
transcription profile of soybean root knot nematode interaction reveals a key role of phythormones in the Resistance Reaction
BMC Genomics, 2013Co-Authors: Magda Aparecida Beneventi, Orzenil Bonfim Da Silva, Alexandre Augusto Pereira Firmino, Regina Maria Santos De Amorim, Erika V S Albuquerque, Maria Cristina Mattar Da Silva, Joseane Padilha Da Silva, Magnolia De Araujo CamposAbstract:Root-knot nematodes (RKN– Meloidogyne genus) present extensive challenges to soybean crop. The soybean line (PI 595099) is known to be resistant against specific strains and races of nematode species, thus its differential gene expression analysis can lead to a comprehensive gene expression profiling in the incompatible soybean-RKN interaction. Even though many disease Resistance genes have been studied, little has been reported about phytohormone crosstalk on modulation of ROS signaling during soybean-RKN interaction. Using 454 technology to explore the common aspects of Resistance Reaction during both parasitism and Resistance phases it was verified that hormone, carbohydrate metabolism and stress related genes were consistently expressed at high levels in infected roots as compared to mock control. Most noteworthy genes include those encoding glycosyltransferases, peroxidases, auxin-responsive proteins and gibberellin-regulated genes. Our data analysis suggests the key role of glycosyltransferases, auxins and components of gibberellin signal transduction, biosynthesis and deactivation pathways in the Resistance Reaction and their participation in jasmonate signaling and redox homeostasis in mediating aspects of plant growth and responses to biotic stress. Based on this study we suggest a reasonable model regarding to the complex mechanisms of crosstalk between plant hormones, mainly gibberellins and auxins, which can be crucial to modulate the levels of ROS in the Resistance Reaction to nematode invasion. The model also includes recent findings concerning to the participation of DELLA-like proteins and ROS signaling controlling plant immune or stress responses. Furthermore, this study provides a dataset of potential candidate genes involved in both nematode parasitism and Resistance, which can be tested further for their role in this biological process using functional genomics approaches.
J. D. Taylor - One of the best experts on this subject based on the ideXlab platform.
-
cultivar specific avirulence and virulence functions assigned to avrpphf in pseudomonas syringae pv phaseolicola the cause of bean halo blight disease
The EMBO Journal, 2000Co-Authors: George Tsiamis, Alan Vivian, J. W. Mansfield, Ruth Hockenhull, Robert W Jackson, Ane Sesma, Evangelos Athanassopoulos, Mark Bennett, Conrad Stevens, J. D. TaylorAbstract:The avrPphF gene was cloned from Pseudomonas syringae pathovar phaseolicola (Pph) races 5 and 7, based on its ability to confer avirulence towards bean cultivars carrying the R1 gene for halo-blight Resistance, such as Red Mexican. avrPphF comprised two open reading frames, which were both required for function, and was located on a 154 kb plasmid (pAV511) in Pph. Strain RW60 of Pph, lacking pAV511, displayed a loss in virulence to a range of previously susceptible cultivars such as Tendergreen and Canadian Wonder. In Tendergreen virulence was restored to RW60 by avrPphF alone, whereas subcloned avrPphF in the absence of pAV511 greatly accelerated the hypersensitive Resistance Reaction caused by RW60 in Canadian Wonder. A second gene from pAV511, avrPphC, which controls avirulence to soybean, was found to block the activity of avrPphF in Canadian Wonder, but not in Red Mexican. avrPphF also conferred virulence in soybean. The multiple functions of avrPphF illustrate how effector proteins from plant pathogens have evolved to be recognized by R gene products and, therefore, be classified as encoded by avirulence genes.
-
Avirulence genes from Pseudomonas syringae pathovars phaseolicola and pisi confer specificity towards both host and non-host species
Physiological and Molecular Plant Pathology, 1992Co-Authors: A. J. Fillingham, Ian R. Crute, Josie R. Bevan, J. Wood, J. D. Taylor, J. W. Mansfield, Alan VivianAbstract:Avirulence genes from Pseudomonas syringae pathovars phaseolicola and pisi which determine specificity towards cultivars of their respective host species (bean and pea), were found also to determine specificity towards the non-host species. Transconjugants of Ps.s. pv. pisi harbouring clones containing avrPph3, previously isolated from race 3 of Ps.s. pv. phaseolicola, induced a hypersensitive Reaction (HR) in all pea cultivars tested. Clones containing avrPpi2, previously isolated from Ps.s. pv. pisi race 2, caused Ps.s. pv. phaseolicola to induce a rapid HR in most cultivars of bean but in cv. Seafarer a markedly slower incompatible response was observed. Transconjugants of the heterologous pathovar harbouring avrPpi2 or avrPph3, therefore, induced Resistance patterns in non-host cultivars which were unlike those of any known race of the homologous pathogen. The quantitatively different Resistance responses of some bean cultivars were further characterized by analyses of phytoalexin accumulation and bacterial multiplication in pod tissue. Responses of F2progeny of crosses between bean cultivars towards transconjugants harbouring avrPpi2 suggested the presence of a dominant allele at a single locus regulating the rapid HR characteristic of Resistance in cv. Canadian Wonder and a dominant allele at an independent second locus regulating the slower Resistance Reaction of cv. Seafarer. It appears that a one gene for two genes relationship is involved in the avrPpi2-bean interaction. Clones carrying two other avirulence genes, avrPph2 and avrPpi3, from Ps.s. pv. phaseolicola and Ps.s. pv. pisi, respectively, had no effect on the virulence of heterologous pathovars towards any of the cultivars tested. © 1992.
Robert W Jackson - One of the best experts on this subject based on the ideXlab platform.
-
exposure to host Resistance mechanisms drives evolution of bacterial virulence in plants
Current Biology, 2005Co-Authors: Andrew R Pitman, John W. Mansfield, Robert W Jackson, Victor Kaitell, Richard Thwaites, Dawn L ArnoldAbstract:Summary Bacterial pathogenicity to plants and animals has evolved through an arms race of attack and defense. Key players are bacterial effector proteins, which are delivered through the type III secretion system and suppress basal defenses [1]. In plants, varietal Resistance to disease is based on recognition of effectors by the products of Resistance ( R ) genes [2]. When recognized, the effector or in this scenario, avirulence (Avr) protein triggers the hypersensitive Resistance Reaction (HR), which generates antimicrobial conditions [3]. Unfortunately, such gene-for-gene-based Resistance commonly fails because of the emergence of virulent strains of the pathogen that no longer trigger the HR [4]. We have followed the emergence of a new virulent pathotype of the halo-blight pathogen Pseudomonas syringae pv. phaseolicola within leaves of a resistant variety of bean. Exposure to the HR led to the selection of strains lacking the avirulence (effector) gene avrPphB (or hopAR1 [5]), which triggers defense in varieties with the matching R3 Resistance gene. Loss of avrPphB was through deletion of a 106 kb genomic island (PPHGI-1) that shares features with integrative and conjugative elements (ICElands) and also pathogenicity islands (PAIs) in diverse bacteria [6, 7]. We provide a molecular explanation of how exposure to Resistance mechanisms in plants drives the evolution of new virulent forms of pathogens.
-
cultivar specific avirulence and virulence functions assigned to avrpphf in pseudomonas syringae pv phaseolicola the cause of bean halo blight disease
The EMBO Journal, 2000Co-Authors: George Tsiamis, Alan Vivian, J. W. Mansfield, Ruth Hockenhull, Robert W Jackson, Ane Sesma, Evangelos Athanassopoulos, Mark Bennett, Conrad Stevens, J. D. TaylorAbstract:The avrPphF gene was cloned from Pseudomonas syringae pathovar phaseolicola (Pph) races 5 and 7, based on its ability to confer avirulence towards bean cultivars carrying the R1 gene for halo-blight Resistance, such as Red Mexican. avrPphF comprised two open reading frames, which were both required for function, and was located on a 154 kb plasmid (pAV511) in Pph. Strain RW60 of Pph, lacking pAV511, displayed a loss in virulence to a range of previously susceptible cultivars such as Tendergreen and Canadian Wonder. In Tendergreen virulence was restored to RW60 by avrPphF alone, whereas subcloned avrPphF in the absence of pAV511 greatly accelerated the hypersensitive Resistance Reaction caused by RW60 in Canadian Wonder. A second gene from pAV511, avrPphC, which controls avirulence to soybean, was found to block the activity of avrPphF in Canadian Wonder, but not in Red Mexican. avrPphF also conferred virulence in soybean. The multiple functions of avrPphF illustrate how effector proteins from plant pathogens have evolved to be recognized by R gene products and, therefore, be classified as encoded by avirulence genes.