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O.s. Imade - One of the best experts on this subject based on the ideXlab platform.
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the nigerian catfish clarias gariepinus pisciculture systems reservoirs of multidrug Resistant Bacteria
Journal of Applied Microbiology, 2018Co-Authors: O.s. Imade, F.i. AkinnibosunAbstract:Aim Control of operations of catfish farmers still lacks in Nigeria due to the absence of catfish inspection programmes by relevant regulatory agencies. Thus, this study aimed at performing comprehensive surveillance of Nigerian catfish pisciculture systems so as to provide empirical evidence on the extent of contamination with multidrug-Resistant Bacteria due to potential abuse of standard operational procedures by catfish farmers. Methods and results One-way analysis of variance was performed on pond water and catfish samples which were randomized with the Wei's-Urn randomization modelling technique. Ceftriaxone-selected Bacterial colonies isolated from catfish and pond water samples collected from nursery and grow-out pond systems were tested for multidrug resistance. Haemocytotoxigenic and Shiga toxigenic virulence traits in multidrug-Resistant colonies, as well as detection of hyper-Ambler class C-(AmpC) beta-lactamase and carbapenemase production, were also performed using phenotypic/molecular methods. Of the 648 ceftriaxone-selected Bacterial isolates examined in this study, 515 isolates were found to be multidrug-Resistant Bacteria, of which 366 isolates were characterized as pathogenic multidrug-Resistant Bacteria. Both nursery and grow-out ponds had high frequencies of Bacterial resistance to ampicillin (86-93%), cotrimoxazole (47-87%) and chloramphenicol (95-100%). However, high frequencies of Bacterial resistance to amoxicillin/clavulanic acid (75-83%) were only observed in the grow-out ponds. Also, hyper-AmpC beta-lactamase-producing multidrug-Resistant Bacteria were only seen in the grow-out ponds. Isolated multidrug-Resistant Bacteria were confirmed as Enterobacter cloacae, Citrobacter freundii, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis. Conclusion The findings in this study corroborate the urgent need for some form of international mediation to urge the Nigerian government and her regulatory agencies to commence monitoring of catfish pisciculture operations since multidrug-Resistant Bacteria could be transmitted to humans. Significance and impact of the study Our study provided empirical evidence that revealed the misuse/abuse of antibiotics during pisciculture operations in Nigeria; thus, constituting these pisciculture systems into reservoirs of multidrug-Resistant Bacteria.
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The Nigerian catfish (Clarias gariepinus) pisciculture systems: reservoirs of multidrug‐Resistant Bacteria
Journal of applied microbiology, 2018Co-Authors: O.s. Imade, F.i. AkinnibosunAbstract:Aim Control of operations of catfish farmers still lacks in Nigeria due to the absence of catfish inspection programmes by relevant regulatory agencies. Thus, this study aimed at performing comprehensive surveillance of Nigerian catfish pisciculture systems so as to provide empirical evidence on the extent of contamination with multidrug-Resistant Bacteria due to potential abuse of standard operational procedures by catfish farmers. Methods and results One-way analysis of variance was performed on pond water and catfish samples which were randomized with the Wei's-Urn randomization modelling technique. Ceftriaxone-selected Bacterial colonies isolated from catfish and pond water samples collected from nursery and grow-out pond systems were tested for multidrug resistance. Haemocytotoxigenic and Shiga toxigenic virulence traits in multidrug-Resistant colonies, as well as detection of hyper-Ambler class C-(AmpC) beta-lactamase and carbapenemase production, were also performed using phenotypic/molecular methods. Of the 648 ceftriaxone-selected Bacterial isolates examined in this study, 515 isolates were found to be multidrug-Resistant Bacteria, of which 366 isolates were characterized as pathogenic multidrug-Resistant Bacteria. Both nursery and grow-out ponds had high frequencies of Bacterial resistance to ampicillin (86-93%), cotrimoxazole (47-87%) and chloramphenicol (95-100%). However, high frequencies of Bacterial resistance to amoxicillin/clavulanic acid (75-83%) were only observed in the grow-out ponds. Also, hyper-AmpC beta-lactamase-producing multidrug-Resistant Bacteria were only seen in the grow-out ponds. Isolated multidrug-Resistant Bacteria were confirmed as Enterobacter cloacae, Citrobacter freundii, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis. Conclusion The findings in this study corroborate the urgent need for some form of international mediation to urge the Nigerian government and her regulatory agencies to commence monitoring of catfish pisciculture operations since multidrug-Resistant Bacteria could be transmitted to humans. Significance and impact of the study Our study provided empirical evidence that revealed the misuse/abuse of antibiotics during pisciculture operations in Nigeria; thus, constituting these pisciculture systems into reservoirs of multidrug-Resistant Bacteria.
F.i. Akinnibosun - One of the best experts on this subject based on the ideXlab platform.
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the nigerian catfish clarias gariepinus pisciculture systems reservoirs of multidrug Resistant Bacteria
Journal of Applied Microbiology, 2018Co-Authors: O.s. Imade, F.i. AkinnibosunAbstract:Aim Control of operations of catfish farmers still lacks in Nigeria due to the absence of catfish inspection programmes by relevant regulatory agencies. Thus, this study aimed at performing comprehensive surveillance of Nigerian catfish pisciculture systems so as to provide empirical evidence on the extent of contamination with multidrug-Resistant Bacteria due to potential abuse of standard operational procedures by catfish farmers. Methods and results One-way analysis of variance was performed on pond water and catfish samples which were randomized with the Wei's-Urn randomization modelling technique. Ceftriaxone-selected Bacterial colonies isolated from catfish and pond water samples collected from nursery and grow-out pond systems were tested for multidrug resistance. Haemocytotoxigenic and Shiga toxigenic virulence traits in multidrug-Resistant colonies, as well as detection of hyper-Ambler class C-(AmpC) beta-lactamase and carbapenemase production, were also performed using phenotypic/molecular methods. Of the 648 ceftriaxone-selected Bacterial isolates examined in this study, 515 isolates were found to be multidrug-Resistant Bacteria, of which 366 isolates were characterized as pathogenic multidrug-Resistant Bacteria. Both nursery and grow-out ponds had high frequencies of Bacterial resistance to ampicillin (86-93%), cotrimoxazole (47-87%) and chloramphenicol (95-100%). However, high frequencies of Bacterial resistance to amoxicillin/clavulanic acid (75-83%) were only observed in the grow-out ponds. Also, hyper-AmpC beta-lactamase-producing multidrug-Resistant Bacteria were only seen in the grow-out ponds. Isolated multidrug-Resistant Bacteria were confirmed as Enterobacter cloacae, Citrobacter freundii, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis. Conclusion The findings in this study corroborate the urgent need for some form of international mediation to urge the Nigerian government and her regulatory agencies to commence monitoring of catfish pisciculture operations since multidrug-Resistant Bacteria could be transmitted to humans. Significance and impact of the study Our study provided empirical evidence that revealed the misuse/abuse of antibiotics during pisciculture operations in Nigeria; thus, constituting these pisciculture systems into reservoirs of multidrug-Resistant Bacteria.
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The Nigerian catfish (Clarias gariepinus) pisciculture systems: reservoirs of multidrug‐Resistant Bacteria
Journal of applied microbiology, 2018Co-Authors: O.s. Imade, F.i. AkinnibosunAbstract:Aim Control of operations of catfish farmers still lacks in Nigeria due to the absence of catfish inspection programmes by relevant regulatory agencies. Thus, this study aimed at performing comprehensive surveillance of Nigerian catfish pisciculture systems so as to provide empirical evidence on the extent of contamination with multidrug-Resistant Bacteria due to potential abuse of standard operational procedures by catfish farmers. Methods and results One-way analysis of variance was performed on pond water and catfish samples which were randomized with the Wei's-Urn randomization modelling technique. Ceftriaxone-selected Bacterial colonies isolated from catfish and pond water samples collected from nursery and grow-out pond systems were tested for multidrug resistance. Haemocytotoxigenic and Shiga toxigenic virulence traits in multidrug-Resistant colonies, as well as detection of hyper-Ambler class C-(AmpC) beta-lactamase and carbapenemase production, were also performed using phenotypic/molecular methods. Of the 648 ceftriaxone-selected Bacterial isolates examined in this study, 515 isolates were found to be multidrug-Resistant Bacteria, of which 366 isolates were characterized as pathogenic multidrug-Resistant Bacteria. Both nursery and grow-out ponds had high frequencies of Bacterial resistance to ampicillin (86-93%), cotrimoxazole (47-87%) and chloramphenicol (95-100%). However, high frequencies of Bacterial resistance to amoxicillin/clavulanic acid (75-83%) were only observed in the grow-out ponds. Also, hyper-AmpC beta-lactamase-producing multidrug-Resistant Bacteria were only seen in the grow-out ponds. Isolated multidrug-Resistant Bacteria were confirmed as Enterobacter cloacae, Citrobacter freundii, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis. Conclusion The findings in this study corroborate the urgent need for some form of international mediation to urge the Nigerian government and her regulatory agencies to commence monitoring of catfish pisciculture operations since multidrug-Resistant Bacteria could be transmitted to humans. Significance and impact of the study Our study provided empirical evidence that revealed the misuse/abuse of antibiotics during pisciculture operations in Nigeria; thus, constituting these pisciculture systems into reservoirs of multidrug-Resistant Bacteria.
Fang Tang - One of the best experts on this subject based on the ideXlab platform.
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Inactivation and reactivation of antibiotic-Resistant Bacteria during and after UV disinfection in reclaimed water
Huan jing ke xue= Huanjing kexue, 2014Co-Authors: Jing-jing Huang, Fang Tang, Yu-chen PangAbstract:Prevalence of antibiotic-Resistant Bacteria in wastewater effluents is concerned as an emerging contaminant. To estimate inactivation and reactivation potentials of antibiotic-Resistant Bacteria by UV disinfection, inactivation and reactivation of penicillin-, ampicillin-, cephalexin-, chloramphenicol-and rifampicin-Resistant Bacteria in the secondary effluent were studied under different UV doses. The results showed that the inactivation ratios of penicillin-, ampicillin-, cephalexin-and chloramphenicol-Resistant Bacteria were higher than 4-log, which was closed to that of total heterotrophic Bacteria; however, the inactivation ratio of rifampicin-Resistant Bacteria was lower (3.7-log) under 20 mJ x cm(-2) UV exposure. After 22 h standing incubation, antibiotic-Resistant Bacteria widely reactivated. The colony forming ability of antibiotic-Resistant Bacteria was as high as 3-log when exposed to 20 mJ x cm(-2) UV light. Hence, conventional UV dose can not effectively control reactivation of antibiotic-Resistant Bacteria in reclaimed water by UV disinfection.
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Monitoring and evaluation of antibiotic-Resistant Bacteria at a municipal wastewater treatment plant in China
Environment International, 2012Co-Authors: Jing-jing Huang, Sun-qin Lu, Yun Lu, Hong-ying Hu, Fang Tang, Yi Li, Bin WeiAbstract:The prevalence of antibiotic-Resistant Bacteria in municipal wastewater treatment plants (WWTPs) is becoming a concern of public health. In order to acquire information on the emission of antibiotic-Resistant Bacteria from WWTP effluents into natural waters, both average antibiotic tolerance and concentrations of antibiotic-Resistant Bacteria in the effluent of a WWTP in Beijing, China were investigated. A new index of IC(50)/MIC ratio (the antibiotic concentration required to inhibit 50% of total heterotrophic Bacteria compared to the highest minimum inhibitory concentration value of a group of pathogens according to a specific antibiotic, as defined by CLSI) was used to reflect the average antibiotic tolerance of total heterotrophic Bacteria in the secondary effluent. The results showed that the IC(50)/MIC ratios of heterotrophic Bacteria in the secondary effluent to penicillin, ampicillin, cephalothin, chloramphenicol and rifampicin were >2, >1, >1, and 1.08, respectively, which reflected a significantly high general level of heterotrophic Bacteria found in the secondary effluent Resistant to these five antibiotics. The concentrations of penicillin-, ampicillin-, cephalothin-, and chloramphenicol-Resistant Bacteria were as high as 1.5×10(4)-1.9×10(5), 1.2×10(4)-1.5×10(5), 8.9×10(3)-1.9×10(5) and 2.6×10(4)-2.0×10(5) CFU/mL, and the average percentages in relation to total heterotrophic Bacteria were 63%, 47%, 55%, and 69%, respectively. The concentrations of tetracycline- and rifampicin-Resistant Bacteria were 840-6.1×10(3) and 310-6.1×10(4) CFU/mL with average percentages of 2.6% and 11%, respectively. Furthermore, our study found that five- and six-antibiotic-Resistant Bacteria were widely distributed in four types of enteroBacteria from the secondary effluent. The presence of multiple-antibiotic-Resistant Bacteria from effluents of WWTPs into natural waters could pose a serious problem as a secondary pollutant of drinking water.
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Inactivation and reactivation of antibiotic-Resistant Bacteria by chlorination in secondary effluents of a municipal wastewater treatment plant.
Water research, 2011Co-Authors: Jing-jing Huang, Fang TangAbstract:Reports state that chlorination of drinking water and wastewater affects the proportions of antibiotic-Resistant Bacteria by potentially assisting in microbial selection. Studies on the effect of chlorination on like species of antibiotic-Resistant Bacteria, however, have shown to be conflicting; furthermore, few studies have inspected the regrowth or reactivation of antibiotic-Resistant Bacteria after chlorination in wastewater. To understand the risks of chlorination resulting from potentially selecting for antibiotic-Resistant Bacteria, inactivation and reactivation rates of both total heterotrophic Bacteria and antibiotic-Resistant Bacteria (including penicillin-, ampicillin-, tetracycline-, chloramphenicol-, and rifampicin-Resistant Bacteria) were examined after chlorinating secondary effluent samples from a municipal wastewater treatment plant in this study. Our experimental results indicated similar inactivation rates of both total heterotrophic Bacteria and antibiotic-Resistant Bacteria. Microbial community composition, however, was affected by chlorination: treating samples with 10 mg Cl(2)/L for 10 min resulted in chloramphenicol-Resistant Bacteria accounting for nearly 100% of the microbial population in contrast to 78% before chlorination. This trend shows that chlorination contributes to selection of some antibiotic-Resistant strains. Reactivation of antibiotic-Resistant Bacteria occurred at 2.0 mg Cl(2)/L for 10 min; specifically, chloramphenicol-, ampicillin-, and penicillin-Resistant Bacteria were the three prevalent groups present, and the reactivation of chloramphenicol-Resistant Bacteria exceeded 50%. Regrowth and reactivation of antibiotic-Resistant Bacteria in secondary effluents after chlorination with a long retention time could threaten public health security during wastewater reuse.
Jing-jing Huang - One of the best experts on this subject based on the ideXlab platform.
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Inactivation and reactivation of antibiotic-Resistant Bacteria during and after UV disinfection in reclaimed water
Huan jing ke xue= Huanjing kexue, 2014Co-Authors: Jing-jing Huang, Fang Tang, Yu-chen PangAbstract:Prevalence of antibiotic-Resistant Bacteria in wastewater effluents is concerned as an emerging contaminant. To estimate inactivation and reactivation potentials of antibiotic-Resistant Bacteria by UV disinfection, inactivation and reactivation of penicillin-, ampicillin-, cephalexin-, chloramphenicol-and rifampicin-Resistant Bacteria in the secondary effluent were studied under different UV doses. The results showed that the inactivation ratios of penicillin-, ampicillin-, cephalexin-and chloramphenicol-Resistant Bacteria were higher than 4-log, which was closed to that of total heterotrophic Bacteria; however, the inactivation ratio of rifampicin-Resistant Bacteria was lower (3.7-log) under 20 mJ x cm(-2) UV exposure. After 22 h standing incubation, antibiotic-Resistant Bacteria widely reactivated. The colony forming ability of antibiotic-Resistant Bacteria was as high as 3-log when exposed to 20 mJ x cm(-2) UV light. Hence, conventional UV dose can not effectively control reactivation of antibiotic-Resistant Bacteria in reclaimed water by UV disinfection.
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Monitoring and evaluation of antibiotic-Resistant Bacteria at a municipal wastewater treatment plant in China
Environment International, 2012Co-Authors: Jing-jing Huang, Sun-qin Lu, Yun Lu, Hong-ying Hu, Fang Tang, Yi Li, Bin WeiAbstract:The prevalence of antibiotic-Resistant Bacteria in municipal wastewater treatment plants (WWTPs) is becoming a concern of public health. In order to acquire information on the emission of antibiotic-Resistant Bacteria from WWTP effluents into natural waters, both average antibiotic tolerance and concentrations of antibiotic-Resistant Bacteria in the effluent of a WWTP in Beijing, China were investigated. A new index of IC(50)/MIC ratio (the antibiotic concentration required to inhibit 50% of total heterotrophic Bacteria compared to the highest minimum inhibitory concentration value of a group of pathogens according to a specific antibiotic, as defined by CLSI) was used to reflect the average antibiotic tolerance of total heterotrophic Bacteria in the secondary effluent. The results showed that the IC(50)/MIC ratios of heterotrophic Bacteria in the secondary effluent to penicillin, ampicillin, cephalothin, chloramphenicol and rifampicin were >2, >1, >1, and 1.08, respectively, which reflected a significantly high general level of heterotrophic Bacteria found in the secondary effluent Resistant to these five antibiotics. The concentrations of penicillin-, ampicillin-, cephalothin-, and chloramphenicol-Resistant Bacteria were as high as 1.5×10(4)-1.9×10(5), 1.2×10(4)-1.5×10(5), 8.9×10(3)-1.9×10(5) and 2.6×10(4)-2.0×10(5) CFU/mL, and the average percentages in relation to total heterotrophic Bacteria were 63%, 47%, 55%, and 69%, respectively. The concentrations of tetracycline- and rifampicin-Resistant Bacteria were 840-6.1×10(3) and 310-6.1×10(4) CFU/mL with average percentages of 2.6% and 11%, respectively. Furthermore, our study found that five- and six-antibiotic-Resistant Bacteria were widely distributed in four types of enteroBacteria from the secondary effluent. The presence of multiple-antibiotic-Resistant Bacteria from effluents of WWTPs into natural waters could pose a serious problem as a secondary pollutant of drinking water.
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Inactivation and reactivation of antibiotic-Resistant Bacteria by chlorination in secondary effluents of a municipal wastewater treatment plant.
Water research, 2011Co-Authors: Jing-jing Huang, Fang TangAbstract:Reports state that chlorination of drinking water and wastewater affects the proportions of antibiotic-Resistant Bacteria by potentially assisting in microbial selection. Studies on the effect of chlorination on like species of antibiotic-Resistant Bacteria, however, have shown to be conflicting; furthermore, few studies have inspected the regrowth or reactivation of antibiotic-Resistant Bacteria after chlorination in wastewater. To understand the risks of chlorination resulting from potentially selecting for antibiotic-Resistant Bacteria, inactivation and reactivation rates of both total heterotrophic Bacteria and antibiotic-Resistant Bacteria (including penicillin-, ampicillin-, tetracycline-, chloramphenicol-, and rifampicin-Resistant Bacteria) were examined after chlorinating secondary effluent samples from a municipal wastewater treatment plant in this study. Our experimental results indicated similar inactivation rates of both total heterotrophic Bacteria and antibiotic-Resistant Bacteria. Microbial community composition, however, was affected by chlorination: treating samples with 10 mg Cl(2)/L for 10 min resulted in chloramphenicol-Resistant Bacteria accounting for nearly 100% of the microbial population in contrast to 78% before chlorination. This trend shows that chlorination contributes to selection of some antibiotic-Resistant strains. Reactivation of antibiotic-Resistant Bacteria occurred at 2.0 mg Cl(2)/L for 10 min; specifically, chloramphenicol-, ampicillin-, and penicillin-Resistant Bacteria were the three prevalent groups present, and the reactivation of chloramphenicol-Resistant Bacteria exceeded 50%. Regrowth and reactivation of antibiotic-Resistant Bacteria in secondary effluents after chlorination with a long retention time could threaten public health security during wastewater reuse.
Xiao Bin - One of the best experts on this subject based on the ideXlab platform.
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Study on the distribution characteristics of tetracyclines Resistant Bacteria in wastewater treatment plant
Environmental Pollution & Control, 2012Co-Authors: Xiao BinAbstract:Spread plate method was used to determinate the distribution of tetracyclines(TCs) Resistant Bacteria in two municipal wastewater treatment plant.The performance of different treatment process for TCs Resistant Bacteria removal was investigated.Three TCs-Resistant Bacteria were detected in both municipal wastewater treatment plants;they were oxytetracycline Resistant Bacteria,tetracycline Resistant Bacteria and chlortetracycline Resistant Bacteria.The conlony density of three TCs-Resistant Bacteria followed the order of xytetracycline Resistant Bacteriatetracycline Resistant Bacteria chlortetracycline Resistant Bacteria.The colony counts of TCs-Resistant Bacteria were smallest in chlorination effluent(about 0-103 cfu/mL) and highest in waste sludge(about 105-107 cfu/mL).The wastewater treatment process had perfect efficiency for tetracycline Resistant Bacteria removal,the colony counts of tetracycline Resistant Bacteria in influent was decreased from 1.0×105-1.6×105 cfu/mL to final effluent of 0-5.5×102 cfu/mL.While,the percentage of tetracycline Resistant Bacteria in total TCs-Resistant Bacteria did not present significantly decline,it was 1.1%-3.4% in influent water and maintained 3.1%-4.3% in final effluent water.It could be concluded that the wastewater treatment process was not selective for enrichment or removal of tetracycline Resistant Bacteria.
