The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Didier Sarazin - One of the best experts on this subject based on the ideXlab platform.
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Comparison of Two Swiss-Designed Hyaluronic Acid Gels: Six-Month Clinical Follow-Up.
Journal of drugs in dermatology : JDD, 2017Co-Authors: Patrick Micheels, Stéphanie Besse, Didier Sarazin, Badwi EliasdAbstract:The aim of this paper is to compare 2 hyaluronic acid gel fillers from the same Swiss manufacturer and with the same indications: filling of line wrinkles and folds. The products differ by their cross-linking process. With very simple easy-to-reproduce tests, cohesivity and resistance to traction forces were examined. Also, both gels were injected under ultrasound control in the mid Reticular Dermis of three subjects. The papules were controlled under ultrasound and biopsies at D0 and D15. Results showed significant differences between the 2 gels in all the tests. The new gel, manufactured with a lower-crosslinking density, seems to benefit from better integration in the tissue of the mid Reticular Dermis and to have a more cohesive nature than its comparator from a previous crosslinking technology. Under clinical observation, the range of new products present excellent tissue integration properties. J Drugs Dermatol. 2017;16(2):154-161. .
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Two Crosslinking Technologies for Superficial Reticular Dermis Injection: A Comparative Ultrasound and Histologic Study.
The Journal of clinical and aesthetic dermatology, 2017Co-Authors: Patrick Micheels, Stéphanie Besse, Didier SarazinAbstract:Background: Few hyaluronic acid fillers have been developed for superficial injection. Objective: To compare the diffusion and integration properties of cohesive polydensified matrix and Vycross® technology hyaluronic acid fillers with lidocaine following injection into the superficial Reticular Dermis. Methods and materials: Two subjects received two injections each of cohesive polydensified matrix and Vycross® hyaluronic acid (0.2mL/site) in the superficial Reticular Dermis of the buttock under ultrasound control. Biopsies were obtained at Days 0, 15, and/or 90. Ultrasound and histologic analyses were performed, plus a series of simple rheological tests. Results: Day 0 ultrasound images showed cohesive polydensified matrix hyaluronic acid homogeneous with the surrounding Dermis. Vycross® hyaluronic acid showed more heterogeneity and some leakage into the hypoDermis. Day 15 and Day 90 images were similar to Day 0. Histologic examination of biopsy tissue showed cohesive polydensified matrix hyaluronic acid homogeneously distributed among collagen fibrils with no visible particles. Vycross® hyaluronic acid appeared as variable-sized pools with a particulate appearance. Neither gel was associated with an inflammatory reaction. Laboratory tests showed cohesive polydensified matrix hyaluronic acid to have greater cohesivity and resistance to traction forces than Vycross®. Conclusion: cohesive polydensified matrix gel with lidocaine is homogeneously distributed following injection in the superficial Reticular Dermis and may be particularly suited for aesthetic indications requiring superficial injection.
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A blanching technique for intradermal injection of the hyaluronic acid Belotero.
Plastic and reconstructive surgery, 2013Co-Authors: Patrick Micheels, Stéphanie Besse, Didier Sarazin, Hema Sundaram, Timothy C. FlynnAbstract:With the proliferation of dermal fillers in the aesthetic workplace have come instructions from various manufacturers regarding dermal placement. Determination of injection needle location in the Dermis has in large part been based on physician expertise, product and needle familiarity, and patient-specific skin characteristics. An understanding of the precise depth of dermal structures may help practitioners improve injection specificity. Unlike other dermal fillers that suggest intradermal and deep dermal injection planes, a new hyaluronic acid with a cohesive polydensified matrix may be more appropriate for the superficial Dermis because of its structure and its high degree of integration into the Dermis. To that end, the authors designed a small study to quantify the depth of the superficial Dermis by means of ultrasound and histology. Using ultrasound resources, the authors determined the depths of the epiDermis, the Dermis, and the Reticular Dermis in the buttocks of six patients; the authors then extrapolated the depth of the superficial Reticular Dermis. Histologic studies of two of the patients showed full integration of the product in the Reticular Dermis. Following determination of injection depths and filler integration, the authors describe a technique ("blanching") for injection of the cohesive polydensified matrix hyaluronic acid into the superficial Dermis. At this time, blanching is appropriate only for injection of the cohesive polydensified matrix hyaluronic acid known as Belotero Balance in the United States, although it may have applications for other hyaluronic acid products outside of the United States.
Patrick Micheels - One of the best experts on this subject based on the ideXlab platform.
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Comparison of Two Swiss-Designed Hyaluronic Acid Gels: Six-Month Clinical Follow-Up.
Journal of drugs in dermatology : JDD, 2017Co-Authors: Patrick Micheels, Stéphanie Besse, Didier Sarazin, Badwi EliasdAbstract:The aim of this paper is to compare 2 hyaluronic acid gel fillers from the same Swiss manufacturer and with the same indications: filling of line wrinkles and folds. The products differ by their cross-linking process. With very simple easy-to-reproduce tests, cohesivity and resistance to traction forces were examined. Also, both gels were injected under ultrasound control in the mid Reticular Dermis of three subjects. The papules were controlled under ultrasound and biopsies at D0 and D15. Results showed significant differences between the 2 gels in all the tests. The new gel, manufactured with a lower-crosslinking density, seems to benefit from better integration in the tissue of the mid Reticular Dermis and to have a more cohesive nature than its comparator from a previous crosslinking technology. Under clinical observation, the range of new products present excellent tissue integration properties. J Drugs Dermatol. 2017;16(2):154-161. .
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Two Crosslinking Technologies for Superficial Reticular Dermis Injection: A Comparative Ultrasound and Histologic Study.
The Journal of clinical and aesthetic dermatology, 2017Co-Authors: Patrick Micheels, Stéphanie Besse, Didier SarazinAbstract:Background: Few hyaluronic acid fillers have been developed for superficial injection. Objective: To compare the diffusion and integration properties of cohesive polydensified matrix and Vycross® technology hyaluronic acid fillers with lidocaine following injection into the superficial Reticular Dermis. Methods and materials: Two subjects received two injections each of cohesive polydensified matrix and Vycross® hyaluronic acid (0.2mL/site) in the superficial Reticular Dermis of the buttock under ultrasound control. Biopsies were obtained at Days 0, 15, and/or 90. Ultrasound and histologic analyses were performed, plus a series of simple rheological tests. Results: Day 0 ultrasound images showed cohesive polydensified matrix hyaluronic acid homogeneous with the surrounding Dermis. Vycross® hyaluronic acid showed more heterogeneity and some leakage into the hypoDermis. Day 15 and Day 90 images were similar to Day 0. Histologic examination of biopsy tissue showed cohesive polydensified matrix hyaluronic acid homogeneously distributed among collagen fibrils with no visible particles. Vycross® hyaluronic acid appeared as variable-sized pools with a particulate appearance. Neither gel was associated with an inflammatory reaction. Laboratory tests showed cohesive polydensified matrix hyaluronic acid to have greater cohesivity and resistance to traction forces than Vycross®. Conclusion: cohesive polydensified matrix gel with lidocaine is homogeneously distributed following injection in the superficial Reticular Dermis and may be particularly suited for aesthetic indications requiring superficial injection.
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A blanching technique for intradermal injection of the hyaluronic acid Belotero.
Plastic and reconstructive surgery, 2013Co-Authors: Patrick Micheels, Stéphanie Besse, Didier Sarazin, Hema Sundaram, Timothy C. FlynnAbstract:With the proliferation of dermal fillers in the aesthetic workplace have come instructions from various manufacturers regarding dermal placement. Determination of injection needle location in the Dermis has in large part been based on physician expertise, product and needle familiarity, and patient-specific skin characteristics. An understanding of the precise depth of dermal structures may help practitioners improve injection specificity. Unlike other dermal fillers that suggest intradermal and deep dermal injection planes, a new hyaluronic acid with a cohesive polydensified matrix may be more appropriate for the superficial Dermis because of its structure and its high degree of integration into the Dermis. To that end, the authors designed a small study to quantify the depth of the superficial Dermis by means of ultrasound and histology. Using ultrasound resources, the authors determined the depths of the epiDermis, the Dermis, and the Reticular Dermis in the buttocks of six patients; the authors then extrapolated the depth of the superficial Reticular Dermis. Histologic studies of two of the patients showed full integration of the product in the Reticular Dermis. Following determination of injection depths and filler integration, the authors describe a technique ("blanching") for injection of the cohesive polydensified matrix hyaluronic acid into the superficial Dermis. At this time, blanching is appropriate only for injection of the cohesive polydensified matrix hyaluronic acid known as Belotero Balance in the United States, although it may have applications for other hyaluronic acid products outside of the United States.
Joseph Mcguire - One of the best experts on this subject based on the ideXlab platform.
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In vitro reconstitution of skin: Fibroblasts facilitate keratinocyte growth and differentiation on acellular Reticular Dermis
The Journal of investigative dermatology, 1991Co-Authors: Niels C. Krejci, Charles B. Cuono, Robert C. Langdon, Joseph McguireAbstract:Abstract Extensive full-thickness burns require replacement of both epiDermis and Dermis. We have described a method in which allogeneic Dermis from engrafted cryopreserved cadaver skin was combined with cultured autologous keratinocytes. In the present study we combined human keratinocytes and fibroblasts, and acellular human, Dermis in vitro and transplanted this "reconstituted skin" into athymic mice. Both human papillary Dermis in which the basement membrane zone has been retained and human Reticular Dermis that has been repopulated with human dermal fibroblasts are good substrates for keratinocyte attachment, stratification, growth, and differentiation. Both of these dermal preparations can be lyophilized and stored at room temperature without losing their ability to support keratinocyte growth. In contrast, human papillary Dermis that has been treated with trypsin lacks laminin and collagen type IV in the BMZ and supports keratinocyte attachment and differentiation less well.
Shigehiko Suzuki - One of the best experts on this subject based on the ideXlab platform.
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combined multiphoton imaging and biaxial tissue extension for quantitative analysis of geometric fiber organization in human Reticular Dermis
Scientific Reports, 2019Co-Authors: Maho Ueda, Susumu Saito, Teruasa Murata, Tomoko Hirano, Ryoma Bise, Kenji Kabashima, Shigehiko SuzukiAbstract:The geometric organization of collagen fibers in human Reticular Dermis and its relationship to that of elastic fibers remain unclear. The tight packing and complex intertwining of dermal collagen fibers hinder accurate analysis of fiber orientation. We hypothesized that combined multiphoton microscopy and biaxial extension could overcome this issue. Continuous observation of fresh dermal sheets under biaxial extension revealed that the geometry of the elastic fiber network is maintained during expansion. Full-thickness human thigh skin samples were biaxially extended and cleared to visualize the entire Reticular Dermis. Throughout the Dermis, collagen fibers straightened with increased inter-fiber spaces, making them more clearly identifiable after extension. The distribution of collagen fibers was evaluated with compilation of local orientation data. Two or three modes were confirmed in all superficial Reticular layer samples. A high degree of local similarities in the direction of collagen and elastic fibers was observed. More than 80% of fibers had directional differences of ≤15°, regardless of layer. Understanding the geometric organization of fibers in the Reticular Dermis improves the understanding of mechanisms underlying the pliability of human skin. Combined multiphoton imaging and biaxial extension provides a research tool for studying the fibrous microarchitecture of the skin.
Luis O Vasconez - One of the best experts on this subject based on the ideXlab platform.
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skin ultrastructure after co2 laser resurfacing
Plastic and Reconstructive Surgery, 1998Co-Authors: Sherry S Collawn, Raymond E Boissy, Luis O VasconezAbstract:Skin ultrastructure was examined in patients undergoing CO2 laser resurfacing for facial rejuvenation. The lasers used in this study were the Coherent Ultrapulse CO2 laser with computerized pattern generator, the Sharplan Feathertouch laser, and the Laserscope Paragon-70 pulsed CO2 laser with computerized pattern generator. Results showed that the epiDermis was totally removed with one pass of the CO2 laser. After one laser pass, there was little compaction of collagen in the Dermis, but after two and three passes, there were sequential graded increases in collagen compaction with loss of the intervening extracellular gel matrix. There was no "collagen shrinkage," and the collagen itself was marginally affected, except for occasional losses in striations at the surface of the specimens. Elastin was very much affected by the laser such that with only one pass, the elastin was abnormal, presenting with a mottled heterogeneous structure. This elastin aberrancy was present in both the papillary and Reticular Dermis. After one laser pass, fibroblast necrosis was present in the papillary Dermis and the Reticular Dermis (depending on which laser was used), and the extent and depth of necrosis increased with multiple laser passes.
