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Nohra E Mateuspinilla - One of the best experts on this subject based on the ideXlab platform.
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metals in obex and Retropharyngeal Lymph Nodes of illinois white tailed deer and their variations associated with cwd status
Prion, 2015Co-Authors: Nelda A Rivera, Jan E Novakofski, Hsinyi Weng, Amy C Kelly, Damian Satterthwaitephillips, Marilyn O Ruiz, Nohra E MateuspinillaAbstract:Prion proteins (PrP(C)) are cell membrane glycoproteins that can be found in many cell types, but specially in neurons. Many studies have suggested PrP(C)'s participation in metal transport and cellular protection against stress in the central nervous system (CNS). On the other hand PrP(Sc), the misfolded isoform of PrP(C) and the pathogenic agent in transmissible spongiform encephalopathies (TSE), has been associated with brain metal dyshomeostasis in prion diseases. Thus, changes in metal concentration associated with protein misfolding and aggregation have been reported for human and animal prion diseases, as well as for other neurodegenerative disorders, such as Parkinson's and Alzheimer's disease. The use of metal concentrations in tissues as surrogate markers for early detection of TSEs has been suggested. Studies on the accumulation of metals in free-ranging white-tailed deer have not been conducted. This study established concentrations of copper, iron, manganese, and magnesium in 2 diagnostic tissues used for CWD testing (obex and Retropharyngeal Lymph Nodes (RLN)). We compared these concentrations between tissues and in relation to CWD status. We established reference intervals (RIs) for these metals and explored their ability to discriminate between CWD-positive and CWD-negative animals. Our results indicate that independent of CWD status, white-tailed deer accumulate higher concentrations of Fe, Mn and Mg in RLN than in obex. White-tailed deer infected with CWD accumulated significantly lower concentrations of Mn and Fe than CWD-negative deer. These patterns differed from other species infected with prion diseases. Overlapping values between CWD positive and negative groups indicate that evaluation of these metals in obex and RLN may not be appropriate as a diagnostic tool for CWD infection in white-tailed deer. Because the CWD-negative deer were included in constructing the RIs, high specificities were expected and should be interpreted with caution. Due to the low sensitivity derived from the RIs, we do not recommend using metal concentrations for disease discrimination.
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metals in obex and Retropharyngeal Lymph Nodes of illinois white tailed deer and their variations associated with cwd status
Prion, 2015Co-Authors: Nelda A Rivera, Jan E Novakofski, Hsinyi Weng, Amy C Kelly, Damian Satterthwaitephillips, Marilyn O Ruiz, Nohra E MateuspinillaAbstract:ABSTRACTPrion proteins (PrPC) are cell membrane glycoproteins that can be found in many cell types, but specially in neurons. Many studies have suggested PrPC‘s participation in metal transport and cellular protection against stress in the central nervous system (CNS). On the other hand PrPSc, the misfolded isoform of PrPC and the pathogenic agent in transmissible spongiform encephalopathies (TSE), has been associated with brain metal dyshomeostasis in prion diseases. Thus, changes in metal concentration associated with protein misfolding and aggregation have been reported for human and animal prion diseases, as well as for other neurodegenerative disorders, such as Parkinson's and Alzheimer's disease. The use of metal concentrations in tissues as surrogate markers for early detection of TSEs has been suggested. Studies on the accumulation of metals in free-ranging white-tailed deer have not been conducted. This study established concentrations of copper, iron, manganese, and magnesium in 2 diagnostic tis...
Li-zhi Liu - One of the best experts on this subject based on the ideXlab platform.
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prognostic value of Retropharyngeal Lymph node metastasis laterality in nasopharyngeal carcinoma and a proposed modification to the uicc ajcc n staging system
Radiotherapy and Oncology, 2019Co-Authors: Ling Huang, Yun Zhang, Yifei Liu, Shunxin Wang, Shaobo Liang, Jian Zhou, Chunyan Cui, Yin Sun, Minyuan Chen, Li-zhi LiuAbstract:Abstract Background and purpose Metastasis laterality is used for N classification of cervical Lymph Nodes, but not Retropharyngeal Lymph Nodes (RLNs). This study explored the prognostic value of laterality of RLN metastasis to provide suggestions for a better N standard classification. Materials and methods This retrospective study evaluated 1225 patients with new biopsy-confirmed nasopharyngeal carcinoma (NPC). Univariable and multivariable Cox regression models were used to assess overall survival (OS), progression-free survival (PFS), and distant metastasis-free survival (DMFS). A new N classification system was developed and compared to the 8th AJCC system. Kaplan-Meier methods with log-rank tests were used to compare OS, PFS, and DMFS between our proposed N stage and the AJCC N stages. Results The incidence of RLN metastasis was 38.7% (unilateral) and 27.5% (bilateral). In the N1 subgroup, metastasis laterality was associated with significant differences in the 5-year rates of OS (89.4% vs. 82.6%, p = 0.016), DMFS (91.5% vs. 82.9%, p = 0.004), and PFS (80.3% vs. 71.2%, p = 0.016). However, no significant differences in these outcomes were observed when we compared N2 disease to N1 bilateral RLN metastasis. Multivariate analysis confirmed that bilateral RLN metastasis independently predicted OS, DMFS, and PFS. The proposed classification broadened the differences in OS, DMFS, PFS between N1 and N2 disease. Conclusion Patients with NPC and unilateral RLN metastasis had better survival than did patients with bilateral RLN metastasis. Upgrading cases with bilateral RLN metastasis from N1 to N2 may help improve prognostication using the 8th AJCC system.
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ultrasound guided fine needle aspiration of Retropharyngeal Lymph Nodes after radiotherapy for nasopharyngeal carcinoma a novel technique for accurate diagnosis
Cancer communications, 2018Co-Authors: Chuanbo Xie, Lin-na Luo, Ke Pan, Xiao-yan Gao, Li-zhi Liu, Jian-ming Gao, Guang-yu Luo, Hong-bo Shan, Mingyuan Chen, Chong ZhaoAbstract:Enlarged Retropharyngeal Lymph Nodes (RLNs) are very common in patients with nasopharyngeal carcinoma (NPC) undergoing radiotherapy. The most suitable treatment option for enlarged RLNs depends on the pathological results. However, RLN sampling is difficult and imminent in the clinic setting. We recently developed a novel minimally invasive technique termed endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for sampling RLN tissues sufficient for pathological or cytological diagnosis. We enrolled 30 post-radiotherapy patients with NPC with suspected RLN metastasis detected via magnetic resonance imaging (MRI). The EUS probe was introduced into the nasopharynx via the nostrils, and EUS was then used to scan the Retropharyngeal space and locate the RLN in the anterior carotid sheath. EUS-FNA was subsequently performed. The safety and efficacy of using EUS-FNA to sample the RLN tissues were assessed. Strips of tissue were successfully sampled from all patients using EUS-FNA. Of the 30 patients, 23 were confirmed to have cancer cells in the biopsied tissues via pathology or cytology examinations with 1 EUS-FNA biopsy session. The seven cases without confirmed cancer cells were subsequently reanalyzed by using another EUS-FNA biopsy session, and two more cases were confirmed possessing cancer cells. The other five patients without confirmed cancer cells were closely followed with MRI every month for 3 months. After follow-up for 3 months, three patients were still considered cancer-free due to the presence of RLNs with stable or shrinking diameters. The rest two patients who showed progressive disease underwent a third EUS-FNA biopsy procedure and were further confirmed to be cancer cell-positive. In the whole cohort reported here, the EUS-FNA procedure was not associated with any severe complications. EUS-FNA is a safe and effective diagnostic approach for sampling tissues from the RLNs in patients with suspected recurrent NPC.
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MOESM1 of Ultrasound-guided fine needle aspiration of Retropharyngeal Lymph Nodes after radiotherapy for nasopharyngeal carcinoma: a novel technique for accurate diagnosis
2018Co-Authors: Chuanbo Xie, Lin-na Luo, Ke Pan, Xiao-yan Gao, Li-zhi Liu, Jian-ming Gao, Guang-yu Luo, Hong-bo ShanAbstract:Additional file 1: Video S1. Sampling of the Retropharyngeal Lymph Nodes with fine needle aspiration under endoscopic ultrasound guidance in patients with post-radiotherapy nasopharyngeal carcinoma
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Ultrasound-guided fine needle aspiration of Retropharyngeal Lymph Nodes after radiotherapy for nasopharyngeal carcinoma: a novel technique for accurate diagnosis
BMC, 2018Co-Authors: Chuanbo Xie, Lin-na Luo, Ke Pan, Xiao-yan Gao, Li-zhi Liu, Jian-ming Gao, Guang-yu Luo, Hong-bo ShanAbstract:Abstract Background Enlarged Retropharyngeal Lymph Nodes (RLNs) are very common in patients with nasopharyngeal carcinoma (NPC) undergoing radiotherapy. The most suitable treatment option for enlarged RLNs depends on the pathological results. However, RLN sampling is difficult and imminent in the clinic setting. We recently developed a novel minimally invasive technique termed endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) for sampling RLN tissues sufficient for pathological or cytological diagnosis. Methods We enrolled 30 post-radiotherapy patients with NPC with suspected RLN metastasis detected via magnetic resonance imaging (MRI). The EUS probe was introduced into the nasopharynx via the nostrils, and EUS was then used to scan the Retropharyngeal space and locate the RLN in the anterior carotid sheath. EUS-FNA was subsequently performed. The safety and efficacy of using EUS-FNA to sample the RLN tissues were assessed. Results Strips of tissue were successfully sampled from all patients using EUS-FNA. Of the 30 patients, 23 were confirmed to have cancer cells in the biopsied tissues via pathology or cytology examinations with 1 EUS-FNA biopsy session. The seven cases without confirmed cancer cells were subsequently reanalyzed by using another EUS-FNA biopsy session, and two more cases were confirmed possessing cancer cells. The other five patients without confirmed cancer cells were closely followed with MRI every month for 3 months. After follow-up for 3 months, three patients were still considered cancer-free due to the presence of RLNs with stable or shrinking diameters. The rest two patients who showed progressive disease underwent a third EUS-FNA biopsy procedure and were further confirmed to be cancer cell-positive. In the whole cohort reported here, the EUS-FNA procedure was not associated with any severe complications. Conclusion EUS-FNA is a safe and effective diagnostic approach for sampling tissues from the RLNs in patients with suspected recurrent NPC
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treatment outcomes and feasibility of partial neck irradiation for patients with nasopharyngeal carcinoma with only Retropharyngeal Lymph node metastasis after intensity modulated radiotherapy
Head and Neck-journal for The Sciences and Specialties of The Head and Neck, 2014Co-Authors: Mo Chen, Li-zhi Liu, Ling-long Tang, Rui Guo, Ying Sun, Yan Ping Mao, Ai-hua LinAbstract:Background The purpose of this study was to summarize the treatment outcomes and evaluate the feasibility of partial neck irradiation in patients with nasopharyngeal carcinoma (NPC) with only Retropharyngeal Lymph Nodes (RLNs) metastasis. Methods Between January 2003 and December 2007, 54 patients with NPC who received partial neck irradiation to levels II, III, and VA and 100 patients who received whole neck irradiation were reviewed. Results The 5-year disease free survival (DFS), disease metastasis-free survival, (DMFS) local relapse-free survival (LRFS), and regional relapse-free survival (RRFS) rates were 81.8%, 87.7%, 94.8%, and 98.1%, respectively. The 5-year RRFS and DFS rates for the partial neck irradiation group and whole neck irradiation group were 98.1% versus 98.0% (p = .882), 87.0% vs 77.0% (p = .117), respectively. Partial neck irradiation was not considered a significant prognostic factor for any endpoint in univariate and multivariate analyses. Conclusion Partial irradiation of neck levels II, III, and VA might be acceptable for patients with NPC with only RLN metastasis. © 2013 Wiley Periodicals, Inc. Head Neck 36: 468–473, 2014
Hans J. Nauwynck - One of the best experts on this subject based on the ideXlab platform.
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Different clinical, virological, serological and tissue tropism outcomes of two new and one old Belgian type 1 subtype 1 porcine reproductive and respiratory virus (PRRSV) isolates
Veterinary Research, 2015Co-Authors: Ilias S Frydas, Ivan Trus, Lise K Kvisgaard, Caroline Bonckaert, Vishwanatha Rap Reddy, Lars E Larsen, Hans J. NauwynckAbstract:In this study, the pathogenic behavior of PRRSV 13V091 and 13V117, isolated in 2013 from two different Belgian farms with enzootic respiratory problems shortly after weaning in the nursery, were compared with the Belgian strain 07V063 isolated in 2007. Full-length genome sequencing was performed to identify their origin. Twelve weeks-old pigs were inoculated intranasally (IN) with 13V091, 13V117 or 07V063 (9 pigs/group). At 10 days post inoculation (dpi), 4 animals from each group were euthanized and tissues were collected for pathology, virological and serological analysis. 13V091 infection resulted in the highest respiratory disease scores and longest period of fever. Gross lung lesions were more pronounced for 13V091 (13%), than for 13V117 (7%) and 07V063 (11%). The nasal shedding and viremia was also most extensive with 13V091. The 13V091 group showed the highest virus replication in conchae, tonsils and Retropharyngeal Lymph Nodes. 13V117 infection resulted in the lowest virus replication in Lymphoid tissues. 13V091 showed higher numbers of sialoadhesin^− infected cells/mm^2 in conchae, tonsils and spleen than 13V117 and 07V063. Neutralizing antibody response with 07V063 was stronger than with 13V091 and 13V117. It can be concluded that (i) 13V091 is a highly pathogenic type 1 subtype 1 PRRSV strain that replicates better than 07V063 and 13V117 and has a strong tropism for sialoadhesin^− cells and (ii) despite the close genetic relationship between 13V117 and 07V063, 13V117 has an increased nasal replication and shedding, but a decreased replication in Lymphoid tissues compared to 07V063.
R Prestwich - One of the best experts on this subject based on the ideXlab platform.
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radiotherapy for oropharyngeal carcinoma with an uninvolved contralateral neck the safety of omission of contralateral high level ii and Retropharyngeal Lymph Nodes from elective target volumes
Clinical Oncology, 2020Co-Authors: Zsuzsanna Iyizobaebozue, L Murray, S Ramasamy, M Sen, P Murray, K Cardale, K Dyker, R PrestwichAbstract:Abstract Aims To analyse outcomes and patterns of failure in patients with oropharyngeal carcinoma (OPC) treated with definitive volumetric modulated arc therapy with omission of contralateral high level II Lymph Nodes (HLII) and Retropharyngeal Lymph Nodes (RPLN) in the contralateral uninvolved neck. Materials and methods Patients with OPC treated between January 2016 and July 2019 were retrospectively identified. In the absence of contralateral neck disease, institutional protocols allowed omission of contralateral HLII and contralateral RPLN in the additional absence of ipsilateral RPLN, soft palate/posterior pharyngeal wall primary. Results In total, 238 patients with OPC and an uninvolved contralateral neck received definitive (chemo)radiotherapy with bilateral neck treatment. The median follow-up was 30.6 months. Two-year local control, regional control and overall survival were 91.0, 91.6 and 86.5%, respectively. Contralateral HLII were omitted in 159/238 (66.8%) patients; this included 106 patients in whom the primary tumour was at/crossed the midline. The contralateral RPLN region was omitted from elective target volumes for 175/238 (73.5%); this included 114 patients with a primary tumour at/crossed the midline. The mean contralateral parotid dose when contralateral HLII and RPLN were both omitted was 24.4 Gy, compared with 28.3 Gy without HLII/RPLN omission (P Conclusion In patients with OPC and an uninvolved contralateral neck receiving bilateral (chemo)radiotherapy, the omission of contralateral RPLN and HLII from elective target volumes was safe and could lead to reduced contralateral parotid doses.
Nelda A Rivera - One of the best experts on this subject based on the ideXlab platform.
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metals in obex and Retropharyngeal Lymph Nodes of illinois white tailed deer and their variations associated with cwd status
Prion, 2015Co-Authors: Nelda A Rivera, Jan E Novakofski, Hsinyi Weng, Amy C Kelly, Damian Satterthwaitephillips, Marilyn O Ruiz, Nohra E MateuspinillaAbstract:Prion proteins (PrP(C)) are cell membrane glycoproteins that can be found in many cell types, but specially in neurons. Many studies have suggested PrP(C)'s participation in metal transport and cellular protection against stress in the central nervous system (CNS). On the other hand PrP(Sc), the misfolded isoform of PrP(C) and the pathogenic agent in transmissible spongiform encephalopathies (TSE), has been associated with brain metal dyshomeostasis in prion diseases. Thus, changes in metal concentration associated with protein misfolding and aggregation have been reported for human and animal prion diseases, as well as for other neurodegenerative disorders, such as Parkinson's and Alzheimer's disease. The use of metal concentrations in tissues as surrogate markers for early detection of TSEs has been suggested. Studies on the accumulation of metals in free-ranging white-tailed deer have not been conducted. This study established concentrations of copper, iron, manganese, and magnesium in 2 diagnostic tissues used for CWD testing (obex and Retropharyngeal Lymph Nodes (RLN)). We compared these concentrations between tissues and in relation to CWD status. We established reference intervals (RIs) for these metals and explored their ability to discriminate between CWD-positive and CWD-negative animals. Our results indicate that independent of CWD status, white-tailed deer accumulate higher concentrations of Fe, Mn and Mg in RLN than in obex. White-tailed deer infected with CWD accumulated significantly lower concentrations of Mn and Fe than CWD-negative deer. These patterns differed from other species infected with prion diseases. Overlapping values between CWD positive and negative groups indicate that evaluation of these metals in obex and RLN may not be appropriate as a diagnostic tool for CWD infection in white-tailed deer. Because the CWD-negative deer were included in constructing the RIs, high specificities were expected and should be interpreted with caution. Due to the low sensitivity derived from the RIs, we do not recommend using metal concentrations for disease discrimination.
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metals in obex and Retropharyngeal Lymph Nodes of illinois white tailed deer and their variations associated with cwd status
Prion, 2015Co-Authors: Nelda A Rivera, Jan E Novakofski, Hsinyi Weng, Amy C Kelly, Damian Satterthwaitephillips, Marilyn O Ruiz, Nohra E MateuspinillaAbstract:ABSTRACTPrion proteins (PrPC) are cell membrane glycoproteins that can be found in many cell types, but specially in neurons. Many studies have suggested PrPC‘s participation in metal transport and cellular protection against stress in the central nervous system (CNS). On the other hand PrPSc, the misfolded isoform of PrPC and the pathogenic agent in transmissible spongiform encephalopathies (TSE), has been associated with brain metal dyshomeostasis in prion diseases. Thus, changes in metal concentration associated with protein misfolding and aggregation have been reported for human and animal prion diseases, as well as for other neurodegenerative disorders, such as Parkinson's and Alzheimer's disease. The use of metal concentrations in tissues as surrogate markers for early detection of TSEs has been suggested. Studies on the accumulation of metals in free-ranging white-tailed deer have not been conducted. This study established concentrations of copper, iron, manganese, and magnesium in 2 diagnostic tis...