Rhabditidae

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Claudia Boehnisch - One of the best experts on this subject based on the ideXlab platform.

Keyun Zhang - One of the best experts on this subject based on the ideXlab platform.

  • serratia nematodiphila sp nov associated symbiotically with the entomopathogenic nematode heterorhabditidoides chongmingensis rhabditida Rhabditidae
    International Journal of Systematic and Evolutionary Microbiology, 2009
    Co-Authors: Chongxing Zhang, Jingrui Liu, Jie Sun, Shouyun Yang, Ren Lai, Huan Liu, Hui Liu, Fei Kan, Jing Sun, Keyun Zhang
    Abstract:

    A novel red-pigmented, Gram-negative, motile, fluorescent, rod-shaped strain, DZ0503SBS1(T), with a single lateral flagellum, was isolated from the intestine of the nematode Heterorhabditidoides chongmingensis. Comparative 16S rRNA gene sequence analysis indicated that the strain is a member of the genus Serratia, sharing highest sequence similarities with Serratia marcescens subsp. sakuensis JCM 11315(T) (99.8 %), S. marcescens subsp. marcescens DSM 30121(T) (99.5 %) and Serratia ureilytica LMG 22860(T) (98.3 %). Similarities between the rpoB gene sequence of strain DZ0503SBS1(T) and those of S. marcescens subsp. sakuensis JCM 11315(T), S. marcescens subsp. marcescens DSM 30121(T) and S. ureilytica LMG 22860(T) were 98.0, 97.4 and 98.3 %, respectively. DNA-DNA hybridization values of strain DZ0503SBS1(T) with S. marcescens subsp. sakuensis JCM 11315(T), S. marcescens subsp. marcescens DSM 30121(T) and S. ureilytica LMG 22860(T) were 68.2, 65.1 and 53.0 %, respectively. The major isoprenoid quinone of strain DZ0503SBS1(T) was Q-8 and the predominant fatty acids were C(16 : 0) (34.76 %), cyclo-C(17 : 0) (20.03 %) and cyclo-C(19 : 0)omega8c (17.24 %). The cyclo-C(19 : 0)omega8c content (17.24 %) was significantly different from those found in S. marcescens subsp. sakuensis JCM 11315(T) and S. marcescens subsp. marcescens DSM 30121(T). Some characteristics of strain DZ0503SBS1(T), i.e. fluorescence and its symbiotic association with nematodes, have not been reported previously in any species of the genus Serratia. Phenotypic and biochemical characteristics and molecular data show that strain DZ0503SBS1(T) represents a novel species, for which the name Serratia nematodiphila sp. nov. is proposed; the type strain is DZ0503SBS1(T) (=KCTC 22130(T) =CGMCC 1.6853(T)).

  • heterorhabditidoides chongmingensis gen nov sp nov rhabditida Rhabditidae a novel member of the entomopathogenic nematodes
    Journal of Invertebrate Pathology, 2008
    Co-Authors: Chongxing Zhang, Jingrui Liu, Jie Sun, Shouyun Yang, Guofu Gao, Maosong Lin, Ren Lai, Keyun Zhang
    Abstract:

    During a recent soil sample survey in Eastern China, a new entomopathogenic nematode species, collected from the Chongming Islands in the southern-eastern area of Shanghai, was discovered. Morphological characteristics of different developmental stages of the nematode combined with molecular data showed that this nematode is a new genus of Rhabditidae, and described as Heterorhabditidoides chongmingensis gen. nov., sp. nov., for that it shares more morphological characteristics with heterorhabditids than with steinernematids. For males, the papillae formula of bursa is 1, 2, 3, 3, with constant papillae number in the terminal group, stoma tubular-shaped and about 1.5 head width; cheilorhabdions cuticularized, esophageal collar present and long, median bulb present. For infective juveniles, EP=90 (80-105)microm, ES=104 (92-120)microm, tail length=111 (89-159)microm, and a=19.1 (15-21). The percentages of the nucleotides A, T, C and G in the ITS1 regions of the new species are significantly different from those of heterorhabditids and other rhabditids. Molecular phylogenetic trees based on 18S rDNA and the internal transcribed spacer (ITS) sequences data revealed that the new entomopathogenic nematode species forms a monophyletic group, which is a sister group of the clade comprised of some genera of Rhabditidae.

Hinrich Schulenburg - One of the best experts on this subject based on the ideXlab platform.

Natsumi Kanzaki - One of the best experts on this subject based on the ideXlab platform.

  • tokorhabditis n gen rhabditida Rhabditidae a comparative nematode model for extremophilic living
    Scientific Reports, 2021
    Co-Authors: Natsumi Kanzaki, Tatsuya Yamashita, James Siho Lee, Peiyin Shih, Erik J Ragsdale, Ryoji Shinya
    Abstract:

    Life in extreme environments is typically studied as a physiological problem, although the existence of extremophilic animals suggests that developmental and behavioral traits might also be adaptive in such environments. Here, we describe a new species of nematode, Tokorhabditis tufae, n. gen., n. sp., which was discovered from the alkaline, hypersaline, and arsenic-rich locale of Mono Lake, California. The new species, which offers a tractable model for studying animal-specific adaptations to extremophilic life, shows a combination of unusual reproductive and developmental traits. Like the recently described sister group Auanema, the species has a trioecious mating system comprising males, females, and self-fertilizing hermaphrodites. Our description of the new genus thus reveals that the origin of this uncommon reproductive mode is even more ancient than previously assumed, and it presents a new comparator for the study of mating-system transitions. However, unlike Auanema and almost all other known rhabditid nematodes, the new species is obligately live-bearing, with embryos that grow in utero, suggesting maternal provisioning during development. Finally, our isolation of two additional, molecularly distinct strains of the new genus-specifically from non-extreme locales-establishes a comparative system for the study of extremophilic traits in this model.

  • phoresy and within colony transmission of nematodes associated with alates of subterranean termites isoptera rhinotermitidae
    Environmental Entomology, 2018
    Co-Authors: Thomas Chouvenc, Jeremiah R Foley, Robin M Giblindavis, Natsumi Kanzaki
    Abstract:

    Termites and their nests are potential resources for a wide assemblage of taxa including nematodes. During dispersal flight events from termite colonies, co-occurring nematodes in the nest may have phoretic opportunities to use termite alates as transportation hosts. The two subterranean termite species Coptotermes gestroi (Wasmann) and Coptotermes formosanus Shiraki are both invasive and established in south Florida. Alates of both species (n = 245) were collected during dispersal flight events in 2015-2016 from six locations, of which 30 (12.2%) were associated with one or more species of nematodes. Species of Bunonema Jagerskiold (Rhabditida: Bunonematidae), Halicephalobus Timm (Rhabditda: Panagrolaimidae), and Poikilolaimus regenfussi (Sudhaus) Sudhaus and Koch (Rhabditida: Rhabditidae) were isolated from 5.3, 4.9, and 0.4% of termite alates, respectively, and Bunonema and Halicephalobus were concomitant in 1.6% of alates. Additional C. formosanus alates were field-collected to establish laboratory colonies in sterilized rearing containers (SRC) to determine if alate-associated nematodes would colonize the newly established nest and/or brood. Among 1-yr-old termite colonies reared in SRCs, 26.9% of the colonies were positive for nematodes confirming that within-colony transmission of nematodes occurred. All three isolated nematode genera are free-living bacterivores capable of asexual reproduction. This suggests that these common co-occurring, termite-associated nematodes are opportunistic and facultative symbionts that receive increased opportunities of geographical dispersion through phoresy during termite dispersal flight events.

  • Poikilolaimus carsiops n. sp. (Rhabditida: Rhabditidae) associated with Neotermes koshunensis (Kalotermitidae) in Kenting National Park, Taiwan
    Nematology, 2011
    Co-Authors: Natsumi Kanzaki, Yen-chiu Lan, Hajime Kosaka, Robin M. Giblin-davis
    Abstract:

    During a survey of termite-associated nematodes in southern Taiwan (Kenting National Park), two species of Poikilolaimus, Poikilolaimus cf. floridensis and an undescribed species, were isolated from termites in the family Kalotermitidae. The undescribed species is morphologically observed, described and figured herein as P. carsiops n. sp. The new species is characterised by an unusual cross-shaped stomatal opening formed by two well developed dorsal and two subventral lip sectors, each with a triangular flap and two vestigial lateral lip sectors lacking a flap, and several other morphological characters. Based upon morphology and molecular phylogenetic analysis, P. carsiops n. sp. is closest to P. floridensis; i. e., these two species share a plesiomorphic regular cuticle and short tail in both males and females, and a lack of metastegostomatal teeth or denticle. In the molecular phylogenetic analysis, P. carsiops n. sp. and P. floridensis formed a well-supported clade which was clearly separated from, but monophyletic with, a clade that includes the other Poikilolaimus species. Because both P. carsiops n. sp. and P. floridensis have been isolated only from the kalotermitid termites, the P. floridensis/P. carsiops n. sp. clade is considered to be specialised with dry wood and damp wood termites (Kalotermitidae).

  • Dauer juveniles of Poikilolaimus floridensis (Rhabditida: Rhabditidae) isolated from drywood termites (Kalotermitidae).
    Nematropica, 2009
    Co-Authors: Natsumi Kanzaki, Robin M. Giblin-davis, Rudolf H. Scheffrahn
    Abstract:

    Kanzaki, N., R. M. Giblin-Davis, R. H. Scheffrahn, and B. J. Center. 2009. Dauer juveniles of Poikilolaimus floridensis (Rhabditida: Rhabditidae) isolated from drywood termites (Kalotermitidae). Nematropica 39:305-310. The dauer juvenile of Poikilolaimus floridensis is described and illustrated. The dauer juveniles were isolated from the foregut of Incisitermes schwarzi (nymphs), I. milleri (alates and workers) and Cryptotermes cavifrons (alates) collected from the upper Florida Keys and identified as P. floridensis using an SSU molecular barcode sequence. The dauers isolated from the termites have a closed stoma and degenerative digestive system, suggesting that the dauer juvenile stage is phoretic and not parasitic. The number of nematodes per infected termite and the infection ratio of termites were low, i.e., three or fewer and 0-33%, respectively. These low numbers and association ratios appear relatively weak for maintenance of the termite-nematode association without local extinctions. Further studies on the life history are needed to understand the dynamics of the association between P. floridensis and kalotermitid termites.

  • Poikilolaimus floridensis n. sp. (Rhabditida: Rhabditidae) associated with termites (Kalotermitidae)
    Nematology, 2009
    Co-Authors: Natsumi Kanzaki, Robin M. Giblin-davis
    Abstract:

    A survey of termite-associated nematodes was done in southern Florida to compare the diversity of such associations with other latitudes in the Neotropics. Six species (15 colonies) of termites (Isoptera) were collected from the field and eight species (15 colonies) from laboratory populations were examined for nematode associates. All six field-collected termite species, Cryptotermes cavifrons, Incisitermes snyderi, Neotermes jouteli, N. castaneus, Prorhinotermes simplex and Reticulitermes flavipes, representing two families (Kalotermitidae and, for the latter two species, Rhinotermitidae), were associated with nematodes. Nematodes were also isolated from laboratory populations of I. snyderi and Coptotermes formosanus. In total, seven putative species of nematodes were discerned using molecular bar-coding and culturing (when successful) including four rhabditids, one diplogastrid, Rhabditis rainai and a nematode that we are describing herein as Poikilolaimus floridensis n. sp. This nematode was isolated as dauer juveniles in the foregut of N. jouteli, N. castaneus and I. snyderi. It was recovered from workers, a soldier and an alate, suggesting internal phoresy. It is characterised by six triangular cuticular flaps covering the stomatal opening, simple tube-like stomatal structure, i.e., absence of teeth and glottoid apparatus, cuticularised and refractile secretory-excretory pore, conical male tail lacking 'bursa' or spike, short and conical female tail and didelphic female reproductive system. Poikilolaimus floridensis n. sp. is morphologically characteristic and does not easily fit the current genus definition of Poikilolaimus which is redefined herein. Molecular phylogenetic analysis based on near full length SSU ribosomal DNA sequence showed that the new species occupies a basal position in the genus.

Chongxing Zhang - One of the best experts on this subject based on the ideXlab platform.

  • serratia nematodiphila sp nov associated symbiotically with the entomopathogenic nematode heterorhabditidoides chongmingensis rhabditida Rhabditidae
    International Journal of Systematic and Evolutionary Microbiology, 2009
    Co-Authors: Chongxing Zhang, Jingrui Liu, Jie Sun, Shouyun Yang, Ren Lai, Huan Liu, Hui Liu, Fei Kan, Jing Sun, Keyun Zhang
    Abstract:

    A novel red-pigmented, Gram-negative, motile, fluorescent, rod-shaped strain, DZ0503SBS1(T), with a single lateral flagellum, was isolated from the intestine of the nematode Heterorhabditidoides chongmingensis. Comparative 16S rRNA gene sequence analysis indicated that the strain is a member of the genus Serratia, sharing highest sequence similarities with Serratia marcescens subsp. sakuensis JCM 11315(T) (99.8 %), S. marcescens subsp. marcescens DSM 30121(T) (99.5 %) and Serratia ureilytica LMG 22860(T) (98.3 %). Similarities between the rpoB gene sequence of strain DZ0503SBS1(T) and those of S. marcescens subsp. sakuensis JCM 11315(T), S. marcescens subsp. marcescens DSM 30121(T) and S. ureilytica LMG 22860(T) were 98.0, 97.4 and 98.3 %, respectively. DNA-DNA hybridization values of strain DZ0503SBS1(T) with S. marcescens subsp. sakuensis JCM 11315(T), S. marcescens subsp. marcescens DSM 30121(T) and S. ureilytica LMG 22860(T) were 68.2, 65.1 and 53.0 %, respectively. The major isoprenoid quinone of strain DZ0503SBS1(T) was Q-8 and the predominant fatty acids were C(16 : 0) (34.76 %), cyclo-C(17 : 0) (20.03 %) and cyclo-C(19 : 0)omega8c (17.24 %). The cyclo-C(19 : 0)omega8c content (17.24 %) was significantly different from those found in S. marcescens subsp. sakuensis JCM 11315(T) and S. marcescens subsp. marcescens DSM 30121(T). Some characteristics of strain DZ0503SBS1(T), i.e. fluorescence and its symbiotic association with nematodes, have not been reported previously in any species of the genus Serratia. Phenotypic and biochemical characteristics and molecular data show that strain DZ0503SBS1(T) represents a novel species, for which the name Serratia nematodiphila sp. nov. is proposed; the type strain is DZ0503SBS1(T) (=KCTC 22130(T) =CGMCC 1.6853(T)).

  • heterorhabditidoides chongmingensis gen nov sp nov rhabditida Rhabditidae a novel member of the entomopathogenic nematodes
    Journal of Invertebrate Pathology, 2008
    Co-Authors: Chongxing Zhang, Jingrui Liu, Jie Sun, Shouyun Yang, Guofu Gao, Maosong Lin, Ren Lai, Keyun Zhang
    Abstract:

    During a recent soil sample survey in Eastern China, a new entomopathogenic nematode species, collected from the Chongming Islands in the southern-eastern area of Shanghai, was discovered. Morphological characteristics of different developmental stages of the nematode combined with molecular data showed that this nematode is a new genus of Rhabditidae, and described as Heterorhabditidoides chongmingensis gen. nov., sp. nov., for that it shares more morphological characteristics with heterorhabditids than with steinernematids. For males, the papillae formula of bursa is 1, 2, 3, 3, with constant papillae number in the terminal group, stoma tubular-shaped and about 1.5 head width; cheilorhabdions cuticularized, esophageal collar present and long, median bulb present. For infective juveniles, EP=90 (80-105)microm, ES=104 (92-120)microm, tail length=111 (89-159)microm, and a=19.1 (15-21). The percentages of the nucleotides A, T, C and G in the ITS1 regions of the new species are significantly different from those of heterorhabditids and other rhabditids. Molecular phylogenetic trees based on 18S rDNA and the internal transcribed spacer (ITS) sequences data revealed that the new entomopathogenic nematode species forms a monophyletic group, which is a sister group of the clade comprised of some genera of Rhabditidae.

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