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Tapani Hovi - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

Pia Laine - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

Carita Savolainen - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

Soile Blomqvist - One of the best experts on this subject based on the ideXlab platform.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

  • phylogenetic analysis of human <B>RhinovirusB> capsid protein vp1 and 2a protease coding sequences confirms shared genus like relationships with human enteroviruses
    2005
    Co-Authors: Pia Laine, Carita Savolainen, Soile Blomqvist, Tapani Hovi
    Abstract:

    Phylogenetic analysis of the capsid protein VP1 coding sequences of all 101 human <B>RhinovirusB> (HRV) prototype strains revealed two major genetic clusters, similar to that of the previously reported VP4/VP2 coding sequences, representing the estaBlished two species, Human <B>RhinovirusB> A (HRV-A) and Human <B>RhinovirusB> B (HRV-B). Pairwise nucleotide identities varied from 61 to 98 % within and from 46 to 55 % Between the two HRV species. Interserotypic sequence identities in Both HRV species were more variaBle than those within any Human enterovirus (HEV) species in the same family. This means that unequivocal serotype identification By VP1 sequence analysis used for HEV strains may not always Be possiBle for HRV isolates. On the other hand, a comprehensive insight into the relationships Between VP1 and partial 2A sequences of HRV and HEV revealed a genus-like situation. DistriBution of pairwise nucleotide identity values Between these genera varied from 41 to 54 % in the VP1 coding region, similar to those Between heterologous memBers of the two HRV species. Alignment of the deduced amino acid sequences revealed more fully conserved amino acid residues Between HRV-B and polioviruses than Between the two HRV species. In phylogenetic trees, where all HRVs and representatives from all HEV species were included, the two HRV species did not cluster together But Behaved like memBers of the same genus as the HEVs. In conclusion, from a phylogenetic point of view, there are no good reasons to keep these two human picornavirus genera taxonomically separated.

Akdis Mübeccel - One of the best experts on this subject based on the ideXlab platform.

  • <B>RhinovirusB> species and tonsillar immune responses
    2019
    Co-Authors: Mikola Emilia, Palomares Oscar, Turunen Riitta, Waris Matti, Ivaska, Lotta E., Silvoniemi Antti, Puhakka Tuomo, Rückert Beate, Vuorinen Tytti, Akdis Mübeccel
    Abstract:

    Background <B>RhinovirusB> A and C infections are important contriButors to asthma induction and exacerBations. No data exist on the interaction of local immune responses in <B>RhinovirusB> infection. Therefore, we aimed to determine the tonsillar immune responses according to <B>RhinovirusB> A, B and C infections. Methods We collected tonsillar samples, nasopharyngeal aspirates and peripheral Blood from 42 <B>RhinovirusB> positive tonsillectomy patients. Fifteen respiratory viruses or their types were investigated from nasopharynx and tonsil tissue, and <B>RhinovirusB> species were typed. The expression of 10 cytokines and 4 transcription factors (IFN-alpha, IFN-Beta, IFN-gamma, IL-10, IL-13, IL-17, IL-28, IL-29, IL-37, TGF-Beta, FOXP3, GATA3, RORC2 and TBet) were studied from tonsil tissue By quantitative PCR. A standard questionnaire of respiratory symptoms and health was filled By the patient or his/her guardian. The patients were divided into three groups By the determination of <B>RhinovirusB> species. Results Overall, 16 patients had <B>RhinovirusB> A, 12 <B>RhinovirusB> B and 14 <B>RhinovirusB> C infection. In <B>RhinovirusB> B positive group there were significantly less men (P = 0.0072), less operated in spring (P = 0.0096) and more operated in fall (P = 0.030) than in <B>RhinovirusB> A or C groups. <B>RhinovirusB> A positive patients had more respiratory symptoms (P = 0.0074) and particularly rhinitis (P = 0.036) on the operation day. There were no significant differences Between the groups in virus codetection. In adjusted analysis, <B>RhinovirusB> C infections were associated with increased IFN-alpha (P = 0.045) and decreased RORC2 expression (P = 0.025). Conclusions <B>RhinovirusB> species associated differently with clinical characteristics and tonsillar cytokine responses.Peer reviewe

  • <B>RhinovirusB> species and tonsillar immune responses
    2019
    Co-Authors: Mikola Emilia, Palomares Oscar, Turunen Riitta, Waris Matti, Ivaska, Lotta E., Silvoniemi Antti, Puhakka Tuomo, Rückert Beate, Vuorinen Tytti, Akdis Mübeccel
    Abstract:

    Background: <B>RhinovirusB> A and C infections are important contriButors to asthma induction and exacerBations. No data exist on the interaction of local immune responses in <B>RhinovirusB> infection. Therefore, we aimed to determine the tonsillar immune responses according to <B>RhinovirusB> A, B and C infections. Methods: We collected tonsillar samples, nasopharyngeal aspirates and peripheral Blood from 42 <B>RhinovirusB> positive tonsillectomy patients. Fifteen respiratory viruses or their types were investigated from nasopharynx and tonsil tissue, and <B>RhinovirusB> species were typed. The expression of 10 cytokines and 4 transcription factors (IFN-α, IFN-β, IFN-γ, IL-10, IL-13, IL-17, IL-28, IL-29, IL-37, TGF-β, FOXP3, GATA3, RORC2 and TBet) were studied from tonsil tissue By quantitative PCR. A standard questionnaire of respiratory symptoms and health was filled By the patient or his/her guardian. The patients were divided into three groups By the determination of <B>RhinovirusB> species. Results: Overall, 16 patients had <B>RhinovirusB> A, 12 <B>RhinovirusB> B and 14 <B>RhinovirusB> C infection. In <B>RhinovirusB> B positive group there were significantly less men (P = 0.0072), less operated in spring (P = 0.0096) and more operated in fall (P = 0.030) than in <B>RhinovirusB> A or C groups. <B>RhinovirusB> A positive patients had more respiratory symptoms (P = 0.0074) and particularly rhinitis (P = 0.036) on the operation day. There were no significant differences Between the groups in virus codetection. In adjusted analysis, <B>RhinovirusB> C infections were associated with increased IFN-α (P = 0.045) and decreased RORC2 expression (P = 0.025). Conclusions: <B>RhinovirusB> species associated differently with clinical characteristics and tonsillar cytokine responses

  • <B>RhinovirusB> Species-Specific AntiBodies Differentially Reflect Clinical Outcomes in Health and Asthma
    2018
    Co-Authors: Megremis Spyridon, Niespodziana Katarzyna, Cabauatan Clarissa, Akdis Mübeccel
    Abstract:

    RATIONALE: <B>RhinovirusB>es are major triggers of common cold and acute asthma exacerBations; <B>RhinovirusB> species A, B and C may have distinct clinical impact; however, little is known regarding RV species-specific antiBody responses in health and asthma. OBJECTIVES: To descriBe and compare total and <B>RhinovirusB> species-specific antiBody levels in healthy and asthmatic children, away from an acute event. METHODS: Serum samples from 163 preschool children with mild to moderate asthma and 72 healthy controls from the multinational Predicta cohort were analysed using the recently developed PreDicta <B>RhinovirusB> antiBody chip. MAIN RESULTS: <B>RhinovirusB> antiBody levels varied, with <B>RhinovirusB> C and <B>RhinovirusB> A Being higher than <B>RhinovirusB> B in Both groups. Compared to controls, asthma was characterised By significantly higher levels of antiBodies to <B>RhinovirusB> A and <B>RhinovirusB> C, But not <B>RhinovirusB> B. <B>RhinovirusB> antiBody levels positively correlated with the numBer of common colds over the previous year in healthy children, and wheeze episodes in asthmatics. AntiBody levels also positively correlated with asthma severity But not with current asthma control. CONCLUSIONS: The variaBle humoral response to <B>RhinovirusB> species in Both groups, suggests a differential infectivity pattern Between <B>RhinovirusB> species. In healthy pre-schoolers, <B>RhinovirusB> antiBodies accumulate with colds. In asthma, <B>RhinovirusB> A and <B>RhinovirusB> C antiBodies are much higher and further increase with disease severity and wheeze episodes. Higher antiBody levels in asthma may Be due to a compromised innate immune response, leading to increased exposure of the adaptive immunity to the virus. Importantly, there is no apparent protection with increasing levels of antiBodies