The Experts below are selected from a list of 300 Experts worldwide ranked by ideXlab platform
Tadashi Kajita - One of the best experts on this subject based on the ideXlab platform.
-
isolation and characterization of microsatellite loci in a mangrove species rhizophora stylosa Rhizophoraceae
Conservation Genetics Resources, 2009Co-Authors: Koji Takayama, Mariko Tamura, Yoichi Tateishi, Tadashi KajitaAbstract:Ten microsatellite markers were isolated from a mangrove species, Rhizophora stylosa (Rhizophoraceae), a widely distributed mangrove plant in the Indo-West Pacific. We tested the variability of the markers on 15–16 individuals of R. stylosa from each of four populations (totaling 63 individuals) and found one to six alleles per locus and population. The range of expected heterozygosity of these markers was 0.000–0.754 in these populations. Cross-species amplification was examined for four congeneric species, R. mucronata, R. apiculata, R. mangle and R. racemosa. Of the ten markers, nine could be amplified in R. mucronata and R. apiculata, and seven in R. mangle and R. racemosa. Seven of them could be used for both Indo-West Pacific species and Atlantic-East Pacific species. Amplification by polymerase chain reaction was optimized under the same parameters across loci, thereby facilitating multiplexing and rapid multi-locus genotyping.
-
isolation and characterization of microsatellite loci in the red mangrove rhizophora mangle Rhizophoraceae and its related species
Conservation Genetics, 2008Co-Authors: Koji Takayama, Mariko Tamura, Yoichi Tateishi, Tadashi KajitaAbstract:Fourteen microsatellite markers were isolated from the red mangrove Rhizophora mangle (Rhizophoraceae), a widely distributed mangrove plant in the New World and West Africa. The range of expected heterozygosity of these markers was 0.000–0.672 in the two populations of R. mangle. Cross-species testing was examined for five other species of Rhizophora, and Kandelia candel and Bruguiera gymnorrhiza. All 14 markers could be amplified in R. samoensis, thirteen in R. racemosa, and six markers in all other species of Rhizophora. Our findings greatly increase the utility of these markers.
Robert E Ricklefs - One of the best experts on this subject based on the ideXlab platform.
-
systematic affinities of Rhizophoraceae and anisophylleaceae and intergeneric relationships within Rhizophoraceae based on chloroplast dna nuclear ribosomal dna and morphology
American Journal of Botany, 2000Co-Authors: Andrea E Schwarzbach, Robert E RicklefsAbstract:A cladistic analysis of sequences from the chloroplast gene rbcL was used to determine the systematic affinities of Rhizophoraceae and Anisophylleaceae. This analysis rejects close relationships of Rhizophoraceae with Celastraceae or Elaeocarpaceae, suggested previously, and identifies Erythroxylaceae as sister group within the Malpighiales, supported by several morphological and anatomical characters. Our molecular results also indicate that Anisophylleaceae are nested within Cucurbitales. Although this placement is novel, this affinity is also well supported by shared morphological characters. Tribal and generic relationships within Rhizophoraceae are evaluated with a combination of six molecular data sets ( rbcL, atpB-rbcL intergenic spacer, trnL-trnF intergenic spacer, ITS1, ITS2, and 5.8S) and a morphological data set. These relationships are compared with results from previous morphological cladistic analyses. Against the background of the molecular results, we briefly discuss the evolution of morphological characters traditionally used for tribal subdivision as well as characters presumably significant for adaptation to mangrove habitats, namely, aerial stilt roots and vivipary.
Koji Takayama - One of the best experts on this subject based on the ideXlab platform.
-
isolation and characterization of 14 microsatellite markers for rhizophora mucronata Rhizophoraceae and their potential use in range wide population studies
Conservation Genetics Resources, 2012Co-Authors: Yoshimi Shinmura, Koji Takayama, Sankararamasubramanian Halasya Meenakshisundaram, Takeshi Asakawa, Bayu Adjie, Erwin Riyanto Ardli, Sarawood Sungkaew, Norhaslinda Binti Malekal, Nguyen Xuan Tung, Severino G SalmoAbstract:A set of 14 new microsatellite markers was developed for mangrove species Rhizophora mucronata (Rhizophoraceae) by using pyrosequencing. Fifty-six samples from 9 populations of R. mucronata in the Indo-West Pacific region were genotyped; all loci were polymorphic, with the number of alleles ranging from 2 to 9. The mean expected heterozygosity per locus was 0.16 in a population from Sabah, no significant linkage disequilibrium was found among loci, and significant deviation from Hardy–Weinberg equilibrium was found in 3 loci. The polymorphic microsatellite markers with samples covering most of the species’ distribution range can be applied in genetic diversity studies covering a broad geographical range of the species.
-
isolation and characterization of microsatellite loci in a mangrove species rhizophora stylosa Rhizophoraceae
Conservation Genetics Resources, 2009Co-Authors: Koji Takayama, Mariko Tamura, Yoichi Tateishi, Tadashi KajitaAbstract:Ten microsatellite markers were isolated from a mangrove species, Rhizophora stylosa (Rhizophoraceae), a widely distributed mangrove plant in the Indo-West Pacific. We tested the variability of the markers on 15–16 individuals of R. stylosa from each of four populations (totaling 63 individuals) and found one to six alleles per locus and population. The range of expected heterozygosity of these markers was 0.000–0.754 in these populations. Cross-species amplification was examined for four congeneric species, R. mucronata, R. apiculata, R. mangle and R. racemosa. Of the ten markers, nine could be amplified in R. mucronata and R. apiculata, and seven in R. mangle and R. racemosa. Seven of them could be used for both Indo-West Pacific species and Atlantic-East Pacific species. Amplification by polymerase chain reaction was optimized under the same parameters across loci, thereby facilitating multiplexing and rapid multi-locus genotyping.
-
isolation and characterization of microsatellite loci in the red mangrove rhizophora mangle Rhizophoraceae and its related species
Conservation Genetics, 2008Co-Authors: Koji Takayama, Mariko Tamura, Yoichi Tateishi, Tadashi KajitaAbstract:Fourteen microsatellite markers were isolated from the red mangrove Rhizophora mangle (Rhizophoraceae), a widely distributed mangrove plant in the New World and West Africa. The range of expected heterozygosity of these markers was 0.000–0.672 in the two populations of R. mangle. Cross-species testing was examined for five other species of Rhizophora, and Kandelia candel and Bruguiera gymnorrhiza. All 14 markers could be amplified in R. samoensis, thirteen in R. racemosa, and six markers in all other species of Rhizophora. Our findings greatly increase the utility of these markers.
Jean Wan Hong Yong - One of the best experts on this subject based on the ideXlab platform.
-
comparative systematic study of colleters and stipules of Rhizophoraceae with implications for adaptation to challenging environments
Botanical Journal of the Linnean Society, 2013Co-Authors: Chiourong Sheue, Peter Chesson, Yingju Chen, Szu Yang Wu, Yeh Hua Wu, Jean Wan Hong Yong, Razafiharimina Marie Agnes Randrianasolo, Mialy Harindra Razanajatovo, Yuen Po YangAbstract:Colleters are multicellular secretory structures found on various organs in flowering plants. Colleters on the adaxial sides of stipules have been hypothesized to play a role in protecting the developing shoot. Rhizophoraceae is a stipulate family with a broad distribution from mangrove to montane environments, which makes the family well suited for the examination of this hypothesis, but the colleters of Rhizophoraceae are not well known. We compared species from all three tribes of Rhizophoraceae, including five inland genera and all four mangrove genera. In all species, several to hundreds of colleters, sessile or stalked, arranged in rows aggregated in genus-specific shapes, are found at the adaxial bases of open and closed stipules. Pellacalyx uniquely has additional colleters at the stipule margins. Colleters are all of the standard type, comprising a central axis of core parenchyma with large vacuoles and tannins, and an outer palisade-like epidermis with organelles involved in secretory activity. An exception is Pellacalyx axillaris, in which colleters appear as extremely small epidermal protrusions. Kandelia obovata has a tracheary element in some colleters. Pellacalyx uniquely has an unusual fleshy outgrowth on the adaxial stipule base. We propose an evolutionary sequence in which Macarisia has plesiomorphic stipule and colleter traits and the mangrove Kandelia obovata with colleter vascular traces is most derived. Colleter and stipule structures are largely concordant with habitat and phylogeny, and show taxonomic value. The strong alignment of colleter and stipule patterns with habitat is suggestive that colleters have a protective function, although some components of these patterns may be phylogenetically determined. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2013, 172, 449–464.
-
molecular phylogeny and evidence for natural hybridization and historical introgression between ceriops species Rhizophoraceae
Biochemical Systematics and Ecology, 2012Co-Authors: Chi Chu Tsai, Peter Chesson, Jean Wan Hong Yong, Yuen Po Yang, Shu Ju Li, Yu Yen Su, Peter Saenger, Glenn WightmanAbstract:Ceriops (Rhizophoraceae) is a genus comprised of five species of mangroves distributed in tropical and subtropical coastal regions. In this study, sequences from nuclear ribosomal ITS and the plastid trnL intron are used to construct molecular phylogenies of this genus revealing two species complexes, the C. tagal complex (C. tagal and C. australis), and the C. decandra complex (C. decandra, C. pseudodecandra and C. zippeliana), each forming a distinct clade. All five species, including the newly designated species C. pseudodecandra, are well supported. However, natural hybridization and historical introgression between Ceriops species are also demonstrated. The ITS sequences of Ceriops species, in contrast to their plastid trnL intron sequences, show a great amount of homoplasy during evolution. Historical introgression originating from natural hybridization was demonstrated based on the additivity of ITS sequences from putative parents. Of the five Ceriops species, C. pseu- dodecandra is a relatively isolated species. C. decandra and C. zippeliana show mutual introgression in most populations. According to both the nuclear ITS sequences and the plastid trnL intron, an intermediate form from Darwin is likelya natural hybrid, with C. tagal and C. australis respectively the maternal and paternal parents.
-
the bruguiera Rhizophoraceae species in the mangroves of singapore especially on the new record and the rediscovery
TAIWANIA, 2005Co-Authors: Chiourong Sheue, Jean Wan Hong Yong, Yuen Po YangAbstract:We reported here the new record of Bruguiera hainesii C. G. Rogers, and the rediscovery of an extinct species, Bruguiera sexangula (Lour.) Poir., in the mangroves of Singapore. To simplify the process of identifying all the five Bruguiera species in Singapore, a colour plate illustrating the calyx structures (across different development stages) and diagnostic features of the five Bruguiera species were provided. A diagnostic key to the five Bruguiera species was also provided, with updated descriptions for the two species. As it was difficult to identify the different Bruguiera species solely on the basis of vegetative structures, we therefore suggest that the series or numbers of colleters (finger-like glandular structures inside the base of stipules) could be an aid for identifying members of Rhizophoraceae especially in the absence of reproductive structures.
-
kandelia obovata Rhizophoraceae a new mangrove species from eastern asia
Taxon, 2003Co-Authors: Chiourong Sheue, Jean Wan Hong YongAbstract:Kandelia (Rhizophoraceae) has long been regarded as a monotypic mangrove genus. Recent studies in chromosome number, molecular phylogeography, physiological adaptation, and leaf anatomy, however, reveal that there are twowell differentiated sets of geographical populations separated by the South China Sea. These are recognized as two distinct species, Kandelia candel (L.) Druce and Kandelia obovata Sheue, Liu & Yong sp. nov.
Yuen Po Yang - One of the best experts on this subject based on the ideXlab platform.
-
comparative systematic study of colleters and stipules of Rhizophoraceae with implications for adaptation to challenging environments
Botanical Journal of the Linnean Society, 2013Co-Authors: Chiourong Sheue, Peter Chesson, Yingju Chen, Szu Yang Wu, Yeh Hua Wu, Jean Wan Hong Yong, Razafiharimina Marie Agnes Randrianasolo, Mialy Harindra Razanajatovo, Yuen Po YangAbstract:Colleters are multicellular secretory structures found on various organs in flowering plants. Colleters on the adaxial sides of stipules have been hypothesized to play a role in protecting the developing shoot. Rhizophoraceae is a stipulate family with a broad distribution from mangrove to montane environments, which makes the family well suited for the examination of this hypothesis, but the colleters of Rhizophoraceae are not well known. We compared species from all three tribes of Rhizophoraceae, including five inland genera and all four mangrove genera. In all species, several to hundreds of colleters, sessile or stalked, arranged in rows aggregated in genus-specific shapes, are found at the adaxial bases of open and closed stipules. Pellacalyx uniquely has additional colleters at the stipule margins. Colleters are all of the standard type, comprising a central axis of core parenchyma with large vacuoles and tannins, and an outer palisade-like epidermis with organelles involved in secretory activity. An exception is Pellacalyx axillaris, in which colleters appear as extremely small epidermal protrusions. Kandelia obovata has a tracheary element in some colleters. Pellacalyx uniquely has an unusual fleshy outgrowth on the adaxial stipule base. We propose an evolutionary sequence in which Macarisia has plesiomorphic stipule and colleter traits and the mangrove Kandelia obovata with colleter vascular traces is most derived. Colleter and stipule structures are largely concordant with habitat and phylogeny, and show taxonomic value. The strong alignment of colleter and stipule patterns with habitat is suggestive that colleters have a protective function, although some components of these patterns may be phylogenetically determined. © 2013 The Linnean Society of London, Botanical Journal of the Linnean Society, 2013, 172, 449–464.
-
molecular phylogeny and evidence for natural hybridization and historical introgression between ceriops species Rhizophoraceae
Biochemical Systematics and Ecology, 2012Co-Authors: Chi Chu Tsai, Peter Chesson, Jean Wan Hong Yong, Yuen Po Yang, Shu Ju Li, Yu Yen Su, Peter Saenger, Glenn WightmanAbstract:Ceriops (Rhizophoraceae) is a genus comprised of five species of mangroves distributed in tropical and subtropical coastal regions. In this study, sequences from nuclear ribosomal ITS and the plastid trnL intron are used to construct molecular phylogenies of this genus revealing two species complexes, the C. tagal complex (C. tagal and C. australis), and the C. decandra complex (C. decandra, C. pseudodecandra and C. zippeliana), each forming a distinct clade. All five species, including the newly designated species C. pseudodecandra, are well supported. However, natural hybridization and historical introgression between Ceriops species are also demonstrated. The ITS sequences of Ceriops species, in contrast to their plastid trnL intron sequences, show a great amount of homoplasy during evolution. Historical introgression originating from natural hybridization was demonstrated based on the additivity of ITS sequences from putative parents. Of the five Ceriops species, C. pseu- dodecandra is a relatively isolated species. C. decandra and C. zippeliana show mutual introgression in most populations. According to both the nuclear ITS sequences and the plastid trnL intron, an intermediate form from Darwin is likelya natural hybrid, with C. tagal and C. australis respectively the maternal and paternal parents.
-
stipules and colleters of the mangrove Rhizophoraceae morphology structure and comparative significance
Botanical Studies, 2012Co-Authors: Chiourong Sheue, Yingju Chen, Yuen Po YangAbstract:One characteristic possessed by the entire mangrove Rhizophoraceae is the strong enclosure of its young shoots by conspicuous rounded or flattened stipules. Young leaves are conspicuously immersed in resinous to milky exudates from colleters (multicellular external secretory emergences) located at the adaxial stipule bases. We systematically studied and compared morphological and structural features of stipules and their colleters from 18 taxa of the mangrove Rhizophoraceae. Three types of sclereid idioblasts (Ceriops and Rhizophora), collenchymas, and thick cuticles, were found to provide a structural basis for the mechanical support of stipules. Several to hundreds (35-580) of finger-like colleters aggregate into genus-specific shapes: rectangular to trapezoidal (Bruguiera), triangular (Ceriops and Kandelia), or as a band (Rhizophora). Number of rows, total number of colleters per stipule, and their individual size, also vary by taxon, and have taxonomic value. All colleters of this subfamily are considered anatomically 'standard', regardless of whether they appear as short-stalked rods (Bruguiera), long-stalked rods (Ceriops, Kandelia) or acuminate rods (Rhizophora, no stalk). Colleters in these taxa are comprised of a central axis of slender, elongated cells and an outer palisade- like epidermis, with a secretory function, perpendicular to the axis. Based on stipule and colleter characteristics, Ceriops and Kandelia are closely related, but Rhizophora shows more derived features. The structural and mechanical protection provided by stipules and colleter exudates may help shield the young shoots of these mangrove plants from their harsh environments.
-
on the morphology and molecular basis of segregation of ceriops zippeliana and c decandra Rhizophoraceae from asia
Blumea, 2009Co-Authors: C R Sheue, C C Tsai, S M A Rashid, J W H Yong, Yuen Po YangAbstract:Ceriops zippeliana, a member of the mangrove Rhizophoraceae, was first reported in 1849. It was considered to be a synonym of C. decandra, which is still widely accepted. We present morphological and molecular evidence to show that C. zippeliana is significantly distinct from C. decandra, and illustrations and an identification key to both species.
-
the bruguiera Rhizophoraceae species in the mangroves of singapore especially on the new record and the rediscovery
TAIWANIA, 2005Co-Authors: Chiourong Sheue, Jean Wan Hong Yong, Yuen Po YangAbstract:We reported here the new record of Bruguiera hainesii C. G. Rogers, and the rediscovery of an extinct species, Bruguiera sexangula (Lour.) Poir., in the mangroves of Singapore. To simplify the process of identifying all the five Bruguiera species in Singapore, a colour plate illustrating the calyx structures (across different development stages) and diagnostic features of the five Bruguiera species were provided. A diagnostic key to the five Bruguiera species was also provided, with updated descriptions for the two species. As it was difficult to identify the different Bruguiera species solely on the basis of vegetative structures, we therefore suggest that the series or numbers of colleters (finger-like glandular structures inside the base of stipules) could be an aid for identifying members of Rhizophoraceae especially in the absence of reproductive structures.