The Experts below are selected from a list of 6246 Experts worldwide ranked by ideXlab platform
Dorothy Trump - One of the best experts on this subject based on the ideXlab platform.
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retinoschisin the x linked retinoschisis protein is a secreted photoreceptor protein and is expressed and released by weri rb1 cells
Human Molecular Genetics, 2000Co-Authors: Celene Grayson, Silvia N M Reid, Adam Rutherford, John R.w. Yates, Debora B Farber, Juliet A Ellis, Jane C. Sowden, Dorothy TrumpAbstract:X-linked retinoschisis is characterized by microcystic-like changes of the macular region and schisis within the inner retinal layers, leading to visual deterioration in males. Many missense and protein-truncating mutations of the causative gene RS1 have now been identified and are thought to be inactivating. RS1 encodes a 224 amino acid protein, retinoschisin, which contains a discoidin domain but is of unknown function. We have generated a polyclonal antibody against a peptide from a unique region within retinoschisin, which detects a protein of similar to 28 kDa in retinal samples reduced with dithiothreitol, but multimers sized >40 kDa under non-reducing conditions. A screen of human tissues with this antibody reveals retinoschisin to be retina specific and the antibody detects a protein of similar size in bovine and murine retinae. We investigated the expression pattern in the retina of both RSI mRNA (using in situ hybridization with Riboprobes) and retinoschisin (using immunohistochemistry), The antisense Riboprobe detected RSI mRNA only in the photoreceptor layer but the protein product of the gene was present both in the photoreceptors and within the inner portions of the retina. Furthermore, differentiated retinoblastoma cells (Weri-Rb1 cells) were found to express RSI mRNA and to release retinoschisin, These results suggest that retinoschisin is released by photoreceptors and has functions within the inner retinal layers. Thus, X-linked retinoschisis is caused by abnormalities in a putative secreted photoreceptor protein and is the first example of a secreted photoreceptor protein associated with a retinal dystrophy.
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Retinoschisin, the X-linked retinoschisis protein, is a secreted photoreceptor protein, and is expressed and released by Weri–Rb1 cells
Human Molecular Genetics, 2000Co-Authors: Celene Grayson, Silvia N M Reid, Adam Rutherford, John R.w. Yates, Debora B Farber, Juliet A Ellis, Jane C. Sowden, Dorothy TrumpAbstract:X-linked retinoschisis is characterized by microcystic-like changes of the macular region and schisis within the inner retinal layers, leading to visual deterioration in males. Many missense and protein-truncating mutations of the causative gene RS1 have now been identified and are thought to be inactivating. RS1 encodes a 224 amino acid protein, retinoschisin, which contains a discoidin domain but is of unknown function. We have generated a polyclonal antibody against a peptide from a unique region within retinoschisin, which detects a protein of similar to 28 kDa in retinal samples reduced with dithiothreitol, but multimers sized >40 kDa under non-reducing conditions. A screen of human tissues with this antibody reveals retinoschisin to be retina specific and the antibody detects a protein of similar size in bovine and murine retinae. We investigated the expression pattern in the retina of both RSI mRNA (using in situ hybridization with Riboprobes) and retinoschisin (using immunohistochemistry), The antisense Riboprobe detected RSI mRNA only in the photoreceptor layer but the protein product of the gene was present both in the photoreceptors and within the inner portions of the retina. Furthermore, differentiated retinoblastoma cells (Weri-Rb1 cells) were found to express RSI mRNA and to release retinoschisin, These results suggest that retinoschisin is released by photoreceptors and has functions within the inner retinal layers. Thus, X-linked retinoschisis is caused by abnormalities in a putative secreted photoreceptor protein and is the first example of a secreted photoreceptor protein associated with a retinal dystrophy.
H. Gert De Couet - One of the best experts on this subject based on the ideXlab platform.
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Whole-mount in situ hybridization of Hawaiian bobtail squid (Euprymna scolopes) embryos with DIG-labeled Riboprobes: II. Embryo preparation, hybridization, washes, and immunohistochemistry.
Cold Spring Harbor protocols, 2009Co-Authors: Patricia N. Lee, Margaret J. Mcfall-ngai, Patrick Callaerts, H. Gert De CouetAbstract:Whole-mount in situ hybridization is a technique used to localize and visualize specific gene transcripts in whole embryos by hybridizing labeled RNA probes complementary to the sequence of interest. A digoxigenin (DIG)-labeled Riboprobe synthesized during in vitro transcription through the incorporation of DIG-labeled UTP is hybridized to the target sequence under stringent conditions, and excess unhybridized probe is removed during a series of washes. The location of the labeled Riboprobe, and thus the mRNA sequence of interest, is then visualized by immunohistochemistry. This protocol outlines the steps involved in preparing Hawaiian bobtail squid (Euprymna scolopes) embryos, hybridizing a DIG-labeled Riboprobe in whole-mount embryos, and visualizing the labeled RNA colorimetrically using an alkaline-phosphatase-conjugated anti-DIG antibody.
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Whole-Mount In Situ Hybridization of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos with DIG-Labeled Riboprobes: I. DNA Template Preparation and In Vitro Transcription of Riboprobes
Cold Spring Harbor protocols, 2009Co-Authors: Patricia N. Lee, Margaret J. Mcfall-ngai, Patrick Callaerts, H. Gert De CouetAbstract:Whole-mount in situ hybridization is a technique used to localize and visualize specific gene transcripts in whole embryos by hybridizing labeled RNA probes complementary to the sequence of interest. A digoxigenin (DIG)-labeled Riboprobe synthesized during in vitro transcription through the incorporation of a DIG-labeled UTP is hybridized to the target sequence under stringent conditions, and excess, unhybridized probe is removed during a series of washes. The location of the labeled Riboprobe, and thus the mRNA sequence of interest, is then visualized by immunohistochemistry. This protocol outlines the techniques for preparing RNA probes for whole-mount in situ hybridization in Hawaiian bobtail squid (Euprymna scolopes) embryos from linearized plasmid DNA or polymerase chain reaction (PCR) products.
Celene Grayson - One of the best experts on this subject based on the ideXlab platform.
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retinoschisin the x linked retinoschisis protein is a secreted photoreceptor protein and is expressed and released by weri rb1 cells
Human Molecular Genetics, 2000Co-Authors: Celene Grayson, Silvia N M Reid, Adam Rutherford, John R.w. Yates, Debora B Farber, Juliet A Ellis, Jane C. Sowden, Dorothy TrumpAbstract:X-linked retinoschisis is characterized by microcystic-like changes of the macular region and schisis within the inner retinal layers, leading to visual deterioration in males. Many missense and protein-truncating mutations of the causative gene RS1 have now been identified and are thought to be inactivating. RS1 encodes a 224 amino acid protein, retinoschisin, which contains a discoidin domain but is of unknown function. We have generated a polyclonal antibody against a peptide from a unique region within retinoschisin, which detects a protein of similar to 28 kDa in retinal samples reduced with dithiothreitol, but multimers sized >40 kDa under non-reducing conditions. A screen of human tissues with this antibody reveals retinoschisin to be retina specific and the antibody detects a protein of similar size in bovine and murine retinae. We investigated the expression pattern in the retina of both RSI mRNA (using in situ hybridization with Riboprobes) and retinoschisin (using immunohistochemistry), The antisense Riboprobe detected RSI mRNA only in the photoreceptor layer but the protein product of the gene was present both in the photoreceptors and within the inner portions of the retina. Furthermore, differentiated retinoblastoma cells (Weri-Rb1 cells) were found to express RSI mRNA and to release retinoschisin, These results suggest that retinoschisin is released by photoreceptors and has functions within the inner retinal layers. Thus, X-linked retinoschisis is caused by abnormalities in a putative secreted photoreceptor protein and is the first example of a secreted photoreceptor protein associated with a retinal dystrophy.
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Retinoschisin, the X-linked retinoschisis protein, is a secreted photoreceptor protein, and is expressed and released by Weri–Rb1 cells
Human Molecular Genetics, 2000Co-Authors: Celene Grayson, Silvia N M Reid, Adam Rutherford, John R.w. Yates, Debora B Farber, Juliet A Ellis, Jane C. Sowden, Dorothy TrumpAbstract:X-linked retinoschisis is characterized by microcystic-like changes of the macular region and schisis within the inner retinal layers, leading to visual deterioration in males. Many missense and protein-truncating mutations of the causative gene RS1 have now been identified and are thought to be inactivating. RS1 encodes a 224 amino acid protein, retinoschisin, which contains a discoidin domain but is of unknown function. We have generated a polyclonal antibody against a peptide from a unique region within retinoschisin, which detects a protein of similar to 28 kDa in retinal samples reduced with dithiothreitol, but multimers sized >40 kDa under non-reducing conditions. A screen of human tissues with this antibody reveals retinoschisin to be retina specific and the antibody detects a protein of similar size in bovine and murine retinae. We investigated the expression pattern in the retina of both RSI mRNA (using in situ hybridization with Riboprobes) and retinoschisin (using immunohistochemistry), The antisense Riboprobe detected RSI mRNA only in the photoreceptor layer but the protein product of the gene was present both in the photoreceptors and within the inner portions of the retina. Furthermore, differentiated retinoblastoma cells (Weri-Rb1 cells) were found to express RSI mRNA and to release retinoschisin, These results suggest that retinoschisin is released by photoreceptors and has functions within the inner retinal layers. Thus, X-linked retinoschisis is caused by abnormalities in a putative secreted photoreceptor protein and is the first example of a secreted photoreceptor protein associated with a retinal dystrophy.
Patricia N. Lee - One of the best experts on this subject based on the ideXlab platform.
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Whole-mount in situ hybridization of Hawaiian bobtail squid (Euprymna scolopes) embryos with DIG-labeled Riboprobes: II. Embryo preparation, hybridization, washes, and immunohistochemistry.
Cold Spring Harbor protocols, 2009Co-Authors: Patricia N. Lee, Margaret J. Mcfall-ngai, Patrick Callaerts, H. Gert De CouetAbstract:Whole-mount in situ hybridization is a technique used to localize and visualize specific gene transcripts in whole embryos by hybridizing labeled RNA probes complementary to the sequence of interest. A digoxigenin (DIG)-labeled Riboprobe synthesized during in vitro transcription through the incorporation of DIG-labeled UTP is hybridized to the target sequence under stringent conditions, and excess unhybridized probe is removed during a series of washes. The location of the labeled Riboprobe, and thus the mRNA sequence of interest, is then visualized by immunohistochemistry. This protocol outlines the steps involved in preparing Hawaiian bobtail squid (Euprymna scolopes) embryos, hybridizing a DIG-labeled Riboprobe in whole-mount embryos, and visualizing the labeled RNA colorimetrically using an alkaline-phosphatase-conjugated anti-DIG antibody.
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Whole-Mount In Situ Hybridization of Hawaiian Bobtail Squid (Euprymna scolopes) Embryos with DIG-Labeled Riboprobes: I. DNA Template Preparation and In Vitro Transcription of Riboprobes
Cold Spring Harbor protocols, 2009Co-Authors: Patricia N. Lee, Margaret J. Mcfall-ngai, Patrick Callaerts, H. Gert De CouetAbstract:Whole-mount in situ hybridization is a technique used to localize and visualize specific gene transcripts in whole embryos by hybridizing labeled RNA probes complementary to the sequence of interest. A digoxigenin (DIG)-labeled Riboprobe synthesized during in vitro transcription through the incorporation of a DIG-labeled UTP is hybridized to the target sequence under stringent conditions, and excess, unhybridized probe is removed during a series of washes. The location of the labeled Riboprobe, and thus the mRNA sequence of interest, is then visualized by immunohistochemistry. This protocol outlines the techniques for preparing RNA probes for whole-mount in situ hybridization in Hawaiian bobtail squid (Euprymna scolopes) embryos from linearized plasmid DNA or polymerase chain reaction (PCR) products.
R. I. Hamilton - One of the best experts on this subject based on the ideXlab platform.
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Detection of sweet potato virus disease-associated closterovirus in a sweet potato accession in the United States
Plant Disease, 1996Co-Authors: G. Pio-ribeiro, Robert L. Jarret, James W. Demski, S. Winter, R. I. HamiltonAbstract:A closterovirus (sweet potato virus disease-associated closterovirus [SPVD-aC]) that, in association with sweet potato feathery mottle potyvirus (SPFMV), causes the Nigerian sweet potato virus disease (SPVD), was detected in sweet potato (Ipomoea setosa) following grafting of infected scions of sweet potato cv. White Bunch, an accession in the USDA sweet potato repository. Confirmation of infection with SPVD-aC was by a Riboprobe hybridization assay specific for the viral RNA coupled with chemiluminescent detection of the bound RNA, and SPFMV was detected by enzyme-linked immunosorbent assay (ELISA) using a specific antiserum. This is the first report of SPVD-aC and of SPVD in sweet potato in North America. Use of the Riboprobe hybridization assay should facilitate a more rapid detection of SPVD-aC infected sweet potato and subsequent selection of virus-free germ plasm for international exchange.
