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Yongjiang Wang - One of the best experts on this subject based on the ideXlab platform.

  • Evidence for seed transmission of Sweet Potato symptomless virus 1 in Sweet Potato ( Ipomoea batatas )
    Journal of Plant Pathology, 2019
    Co-Authors: Qi Qiao, Yongjiang Wang, Yuting Tian, Desheng Zhang, Yanhong Qin, Xiaoli Zhao, Zhenchen Zhang, Shuang Wang, Zhao Fumei
    Abstract:

    Sweet Potato symptomless virus 1 (SPSMV-1) is a member of genus Mastrevirus, in the family Geminiviridae. SPSMV-1 was detected from Sweet Potato (Ipomoea batatas) seedlings by small-RNA deep-sequencing. PCR analysis indicated that SPSMV-1 was detected in 12 out of 114 individual Sweet Potato seedlings from seeds collected from a SPSMV-1-infected Sweet Potato plant, and additionally, detected in whole seeds, seed coats attached with endosperm, and in embryos. The complete genome of the virus was subsequently cloned by using a polymerase chain reaction (PCR) method with back-to-back (full) primer pairs from Sweet Potato seedlings and seeds. In summary, the results suggest that SPSMV-1 may be a seed-borne virus and a seed transmissible virus in Sweet Potato. To our knowledge, this is the first evidence for seed transmission of SPSMV-1, and seed transmission under natural conditions for any mastrevirus as well.

  • Molecular variability of Sweet Potato chlorotic stunt virus (SPCSV) and five potyviruses infecting Sweet Potato in China
    Archives of virology, 2012
    Co-Authors: Yanhong Qin, Yuting Tian, Qi Qiao, Desheng Zhang, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    To obtain a better understanding of the molecular variation of Sweet Potato viruses in China, 131 samples were collected from symptomatic Sweet Potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of Sweet Potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect Sweet Potato in China, and three different strains of SPFMV and two of SPVG were found.

  • First report of Sweet Potato chlorotic stunt virus infecting Sweet Potato in China.
    Plant Disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Zhengqun Zhang, Desheng Zhang, Yanhong Qin, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5′-ATGRMTACTGRCAAAGTAAACGATG-3′) and SPSP4 (5′-TCAACAGTGAAGACCRGYACCRGTCAA-3′) corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 7...

  • First Report of Sweet Potato chlorotic stunt virus Infecting Sweet Potato in China.
    Plant disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Desheng Zhang, Yanhong Qin, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5'-ATGRMTACTGRCAAAGTAAACGATG-3') and SPSP4 (5'-TCAACAGTGAAGACCRGYACCRGTCAA-3') corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 774-bp fragments obtained by amplification were purified and cloned into the PMD19-T vector (TaKaRa, Dalian, China). Recombinant plasmids were then transformed into competent cells of Escherichia coli strain Jm109. Sequencing of the fragments from two individually clones plasmids yielded a nucleotide sequence (GenBank Accession No. HM773432) with 97.4 to 98.7% similarity to the CP gene of East African SPCSV isolates (1). To our knowledge, this is the first report of SPCSV in Sweet Potato fields from China. This virus may threaten Sweet Potato production in China, so cultivars and germplasm should be evaluated for resistance. References: (1) V. Aritua et al. J. Phytopathol. 156:181, 2008. (2) R. W. Njeru et al. Ann. Appl. Biol. 145:71, 2004. (3) G. A. Schaefer and E. R. Terry. Phytopathology 66:642, 1976.

Qi Qiao - One of the best experts on this subject based on the ideXlab platform.

  • Seed transmission of Sweet Potato pakakuy virus in Sweet Potato ( Ipomoea batatas )
    Journal of General Plant Pathology, 2020
    Co-Authors: Fumei Zhao, Yuting Tian, Qi Qiao, Desheng Zhang, Yanhong Qin, Lei Wang, Zhenchen Zhang, Yongjing Wang, Shuang Wang, Xiaoli Zhao
    Abstract:

    Sweet Potato pakakuy virus (SPPV), a member of the species Sweet Potato pakakuy virus (genus Badnavirus, family Caulimoviridae), was recently identified as a Sweet Potato-infecting virus; thus, relatively little is known about the virus and disease epidemiology. In a study on transmission of SPPV by seeds, polymerase chain reaction (PCR) analyses with SPPV-specific primers were used to monitor two strains of SPPV, Sweet Potato badnavirus A (SPBV-A, synonym SPPV-A) and Sweet Potato badnavirus B (SPBV-B, synonym SPPV-B), in seeds and seedlings produced from SPPV-infected plants of two Sweet Potato cultivars (Yu-8 and Xu25-2). SPPV was detected at high frequency in seed tissues including intact seed, embryo, endosperm, and seed coat. All seeds derived from infected Sweet Potato plants gave rise to infected seedlings. Meanwhile, bioinformatics analyses of small RNAs in SPPV-positive seedlings, as verified by PCR, revealed key features of SPPV, such as coat protein, reverse transcriptase, and RNaseH domains, further confirming the seed transmission of SPPV. That SPPV can be transmitted via seeds at a high transmission rate in Sweet Potato has important implications for Sweet Potato breeding and establishment of appropriate strategies for SPPV control.

  • Evidence for seed transmission of Sweet Potato symptomless virus 1 in Sweet Potato ( Ipomoea batatas )
    Journal of Plant Pathology, 2019
    Co-Authors: Qi Qiao, Yongjiang Wang, Yuting Tian, Desheng Zhang, Yanhong Qin, Xiaoli Zhao, Zhenchen Zhang, Shuang Wang, Zhao Fumei
    Abstract:

    Sweet Potato symptomless virus 1 (SPSMV-1) is a member of genus Mastrevirus, in the family Geminiviridae. SPSMV-1 was detected from Sweet Potato (Ipomoea batatas) seedlings by small-RNA deep-sequencing. PCR analysis indicated that SPSMV-1 was detected in 12 out of 114 individual Sweet Potato seedlings from seeds collected from a SPSMV-1-infected Sweet Potato plant, and additionally, detected in whole seeds, seed coats attached with endosperm, and in embryos. The complete genome of the virus was subsequently cloned by using a polymerase chain reaction (PCR) method with back-to-back (full) primer pairs from Sweet Potato seedlings and seeds. In summary, the results suggest that SPSMV-1 may be a seed-borne virus and a seed transmissible virus in Sweet Potato. To our knowledge, this is the first evidence for seed transmission of SPSMV-1, and seed transmission under natural conditions for any mastrevirus as well.

  • Molecular variability of Sweet Potato chlorotic stunt virus (SPCSV) and five potyviruses infecting Sweet Potato in China
    Archives of virology, 2012
    Co-Authors: Yanhong Qin, Yuting Tian, Qi Qiao, Desheng Zhang, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    To obtain a better understanding of the molecular variation of Sweet Potato viruses in China, 131 samples were collected from symptomatic Sweet Potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of Sweet Potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect Sweet Potato in China, and three different strains of SPFMV and two of SPVG were found.

  • First report of Sweet Potato chlorotic stunt virus infecting Sweet Potato in China.
    Plant Disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Zhengqun Zhang, Desheng Zhang, Yanhong Qin, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5′-ATGRMTACTGRCAAAGTAAACGATG-3′) and SPSP4 (5′-TCAACAGTGAAGACCRGYACCRGTCAA-3′) corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 7...

  • First Report of Sweet Potato chlorotic stunt virus Infecting Sweet Potato in China.
    Plant disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Desheng Zhang, Yanhong Qin, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5'-ATGRMTACTGRCAAAGTAAACGATG-3') and SPSP4 (5'-TCAACAGTGAAGACCRGYACCRGTCAA-3') corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 774-bp fragments obtained by amplification were purified and cloned into the PMD19-T vector (TaKaRa, Dalian, China). Recombinant plasmids were then transformed into competent cells of Escherichia coli strain Jm109. Sequencing of the fragments from two individually clones plasmids yielded a nucleotide sequence (GenBank Accession No. HM773432) with 97.4 to 98.7% similarity to the CP gene of East African SPCSV isolates (1). To our knowledge, this is the first report of SPCSV in Sweet Potato fields from China. This virus may threaten Sweet Potato production in China, so cultivars and germplasm should be evaluated for resistance. References: (1) V. Aritua et al. J. Phytopathol. 156:181, 2008. (2) R. W. Njeru et al. Ann. Appl. Biol. 145:71, 2004. (3) G. A. Schaefer and E. R. Terry. Phytopathology 66:642, 1976.

Rodrigo A Valverde - One of the best experts on this subject based on the ideXlab platform.

  • First Report of Sweet Potato virus G and Sweet Potato virus 2 Infecting Sweet Potato in Spain
    Plant Disease, 2007
    Co-Authors: Helena P. Trenado, Gloria Lozano, Rodrigo A Valverde, Jesús Navas-castillo
    Abstract:

    Sweet Potato feathery mottle virus (SPFMV), Sweet Potato virus G (SPVG), and Sweet Potato virus 2 (SPV2) (also known as Ipomoea vein mosaic virus (2) and Sweet Potato virus Y) are members of the genus Potyvirus (family Potyviridae), which can synergistically interact with Sweet Potato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae), increasing symptom severity on Sweet Potato (Ipomoea batatas (L.) Lam.) (1,2,3). During 2002, 2006, and 2007, vine cuttings from Sweet Potato plants were collected in Malaga (southern Spain), Tenerife, and Lanzarote (Canary Islands, Spain) to be tested for the presence of viruses. Sampled plants ranged from asymptomatic to severely affected by symptoms of Sweet Potato virus disease (SPVD), caused by dual infection with SPFMV or other potyviruses with SPCSV. Scions collected during 2002 were grafted to the indicator host I. setosa. Foliar samples from I. setosa were used for nitrocellulose membrane (NCM)-ELISA testing with antisera specific to SPVG or SP...

  • First report of Sweet Potato chlorotic stunt virus and Sweet Potato feathery mottle virus infecting Sweet Potato in Spain.
    Plant Disease, 2004
    Co-Authors: Rodrigo A Valverde, Gloria Lozano, A. Ramos-paz, Jesús Navas-castillo, F Valdés
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV), family Closteroviridae and Sweet Potato feathery mottle virus (SPFMV), family Potyviridae are whitefly and aphid transmitted, respectively, which in double infections cause Sweet Potato virus disease (SPVD) that is a serious Sweet Potato (Ipomoea batatas Lam.) disease in Africa (2). During the past decade, Sweet Potato plants showing symptoms similar to SPVD have been observed in most areas of Spain. Nevertheless, not much information is available about the identity of the viruses infecting this crop in Spain. During the summer of 2002, Sweet Potato plants with foliar mosaic, stunting, leaf malformation, chlorosis, and ringspot symptoms were observed in several farms in Malaga (southern Spain) and Tenerife and Lanzarote (Canary Islands, Spain). Vine cuttings were collected from 21 symptomatic plants in Malaga and from eight plants on Lanzarote and six on Tenerife. Scions were grafted to the indicator hosts, Brazilian morning glory (I. setosa) and I. nil cv. Scar...

  • Whitefly transmission of Sweet Potato viruses
    Virus research, 2004
    Co-Authors: Rodrigo A Valverde, Jeonggu Sim, Pongtharin Lotrakul
    Abstract:

    Abstract Three genera of plant viruses, Begomovirus (Geminiviridae), Crinivirus (Closteroviridae) and Ipomovirus (Potyviridae), contain members that infect Sweet Potato (Ipomoea batatas) and are transmitted by whiteflies. The begomoviruses, Sweet Potato leaf curl virus (SPLCV) and Ipomoea leaf curl virus (ILCV), and the ipomovirus Sweet Potato mild mottle virus are transmitted by Bemisia tabaci, the Sweet Potato whitefly. The crinivirus, Sweet Potato chlorotic stunt virus (SPCSV), is transmitted by B. tabaci and Trialeurodes abutilonea, the bandedwinged whitefly. Transmission experiments were done with three of these viruses using laboratory-reared whiteflies and 2-day acquisition and transmission feeding periods. SPLCV and ILCV were transmitted from single and double infections by B. tabaci at rates of 5–10%. Transmission rates for SPLCV by B. tabaci were 15–20%. T. abutilonea transmitted SPCSV at a rate of ca. 3% but did not transmit ILCV or SPLCV.

Yanhong Qin - One of the best experts on this subject based on the ideXlab platform.

  • Seed transmission of Sweet Potato pakakuy virus in Sweet Potato ( Ipomoea batatas )
    Journal of General Plant Pathology, 2020
    Co-Authors: Fumei Zhao, Yuting Tian, Qi Qiao, Desheng Zhang, Yanhong Qin, Lei Wang, Zhenchen Zhang, Yongjing Wang, Shuang Wang, Xiaoli Zhao
    Abstract:

    Sweet Potato pakakuy virus (SPPV), a member of the species Sweet Potato pakakuy virus (genus Badnavirus, family Caulimoviridae), was recently identified as a Sweet Potato-infecting virus; thus, relatively little is known about the virus and disease epidemiology. In a study on transmission of SPPV by seeds, polymerase chain reaction (PCR) analyses with SPPV-specific primers were used to monitor two strains of SPPV, Sweet Potato badnavirus A (SPBV-A, synonym SPPV-A) and Sweet Potato badnavirus B (SPBV-B, synonym SPPV-B), in seeds and seedlings produced from SPPV-infected plants of two Sweet Potato cultivars (Yu-8 and Xu25-2). SPPV was detected at high frequency in seed tissues including intact seed, embryo, endosperm, and seed coat. All seeds derived from infected Sweet Potato plants gave rise to infected seedlings. Meanwhile, bioinformatics analyses of small RNAs in SPPV-positive seedlings, as verified by PCR, revealed key features of SPPV, such as coat protein, reverse transcriptase, and RNaseH domains, further confirming the seed transmission of SPPV. That SPPV can be transmitted via seeds at a high transmission rate in Sweet Potato has important implications for Sweet Potato breeding and establishment of appropriate strategies for SPPV control.

  • Evidence for seed transmission of Sweet Potato symptomless virus 1 in Sweet Potato ( Ipomoea batatas )
    Journal of Plant Pathology, 2019
    Co-Authors: Qi Qiao, Yongjiang Wang, Yuting Tian, Desheng Zhang, Yanhong Qin, Xiaoli Zhao, Zhenchen Zhang, Shuang Wang, Zhao Fumei
    Abstract:

    Sweet Potato symptomless virus 1 (SPSMV-1) is a member of genus Mastrevirus, in the family Geminiviridae. SPSMV-1 was detected from Sweet Potato (Ipomoea batatas) seedlings by small-RNA deep-sequencing. PCR analysis indicated that SPSMV-1 was detected in 12 out of 114 individual Sweet Potato seedlings from seeds collected from a SPSMV-1-infected Sweet Potato plant, and additionally, detected in whole seeds, seed coats attached with endosperm, and in embryos. The complete genome of the virus was subsequently cloned by using a polymerase chain reaction (PCR) method with back-to-back (full) primer pairs from Sweet Potato seedlings and seeds. In summary, the results suggest that SPSMV-1 may be a seed-borne virus and a seed transmissible virus in Sweet Potato. To our knowledge, this is the first evidence for seed transmission of SPSMV-1, and seed transmission under natural conditions for any mastrevirus as well.

  • Molecular variability of Sweet Potato chlorotic stunt virus (SPCSV) and five potyviruses infecting Sweet Potato in China
    Archives of virology, 2012
    Co-Authors: Yanhong Qin, Yuting Tian, Qi Qiao, Desheng Zhang, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    To obtain a better understanding of the molecular variation of Sweet Potato viruses in China, 131 samples were collected from symptomatic Sweet Potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of Sweet Potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect Sweet Potato in China, and three different strains of SPFMV and two of SPVG were found.

  • First report of Sweet Potato chlorotic stunt virus infecting Sweet Potato in China.
    Plant Disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Zhengqun Zhang, Desheng Zhang, Yanhong Qin, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5′-ATGRMTACTGRCAAAGTAAACGATG-3′) and SPSP4 (5′-TCAACAGTGAAGACCRGYACCRGTCAA-3′) corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 7...

  • First Report of Sweet Potato chlorotic stunt virus Infecting Sweet Potato in China.
    Plant disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Desheng Zhang, Yanhong Qin, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5'-ATGRMTACTGRCAAAGTAAACGATG-3') and SPSP4 (5'-TCAACAGTGAAGACCRGYACCRGTCAA-3') corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 774-bp fragments obtained by amplification were purified and cloned into the PMD19-T vector (TaKaRa, Dalian, China). Recombinant plasmids were then transformed into competent cells of Escherichia coli strain Jm109. Sequencing of the fragments from two individually clones plasmids yielded a nucleotide sequence (GenBank Accession No. HM773432) with 97.4 to 98.7% similarity to the CP gene of East African SPCSV isolates (1). To our knowledge, this is the first report of SPCSV in Sweet Potato fields from China. This virus may threaten Sweet Potato production in China, so cultivars and germplasm should be evaluated for resistance. References: (1) V. Aritua et al. J. Phytopathol. 156:181, 2008. (2) R. W. Njeru et al. Ann. Appl. Biol. 145:71, 2004. (3) G. A. Schaefer and E. R. Terry. Phytopathology 66:642, 1976.

Yuting Tian - One of the best experts on this subject based on the ideXlab platform.

  • Seed transmission of Sweet Potato pakakuy virus in Sweet Potato ( Ipomoea batatas )
    Journal of General Plant Pathology, 2020
    Co-Authors: Fumei Zhao, Yuting Tian, Qi Qiao, Desheng Zhang, Yanhong Qin, Lei Wang, Zhenchen Zhang, Yongjing Wang, Shuang Wang, Xiaoli Zhao
    Abstract:

    Sweet Potato pakakuy virus (SPPV), a member of the species Sweet Potato pakakuy virus (genus Badnavirus, family Caulimoviridae), was recently identified as a Sweet Potato-infecting virus; thus, relatively little is known about the virus and disease epidemiology. In a study on transmission of SPPV by seeds, polymerase chain reaction (PCR) analyses with SPPV-specific primers were used to monitor two strains of SPPV, Sweet Potato badnavirus A (SPBV-A, synonym SPPV-A) and Sweet Potato badnavirus B (SPBV-B, synonym SPPV-B), in seeds and seedlings produced from SPPV-infected plants of two Sweet Potato cultivars (Yu-8 and Xu25-2). SPPV was detected at high frequency in seed tissues including intact seed, embryo, endosperm, and seed coat. All seeds derived from infected Sweet Potato plants gave rise to infected seedlings. Meanwhile, bioinformatics analyses of small RNAs in SPPV-positive seedlings, as verified by PCR, revealed key features of SPPV, such as coat protein, reverse transcriptase, and RNaseH domains, further confirming the seed transmission of SPPV. That SPPV can be transmitted via seeds at a high transmission rate in Sweet Potato has important implications for Sweet Potato breeding and establishment of appropriate strategies for SPPV control.

  • Evidence for seed transmission of Sweet Potato symptomless virus 1 in Sweet Potato ( Ipomoea batatas )
    Journal of Plant Pathology, 2019
    Co-Authors: Qi Qiao, Yongjiang Wang, Yuting Tian, Desheng Zhang, Yanhong Qin, Xiaoli Zhao, Zhenchen Zhang, Shuang Wang, Zhao Fumei
    Abstract:

    Sweet Potato symptomless virus 1 (SPSMV-1) is a member of genus Mastrevirus, in the family Geminiviridae. SPSMV-1 was detected from Sweet Potato (Ipomoea batatas) seedlings by small-RNA deep-sequencing. PCR analysis indicated that SPSMV-1 was detected in 12 out of 114 individual Sweet Potato seedlings from seeds collected from a SPSMV-1-infected Sweet Potato plant, and additionally, detected in whole seeds, seed coats attached with endosperm, and in embryos. The complete genome of the virus was subsequently cloned by using a polymerase chain reaction (PCR) method with back-to-back (full) primer pairs from Sweet Potato seedlings and seeds. In summary, the results suggest that SPSMV-1 may be a seed-borne virus and a seed transmissible virus in Sweet Potato. To our knowledge, this is the first evidence for seed transmission of SPSMV-1, and seed transmission under natural conditions for any mastrevirus as well.

  • Molecular variability of Sweet Potato chlorotic stunt virus (SPCSV) and five potyviruses infecting Sweet Potato in China
    Archives of virology, 2012
    Co-Authors: Yanhong Qin, Yuting Tian, Qi Qiao, Desheng Zhang, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    To obtain a better understanding of the molecular variation of Sweet Potato viruses in China, 131 samples were collected from symptomatic Sweet Potato plants and used for RT-PCR analysis of the heat shock protein 70 (hsp70) gene sequence of Sweet Potato chlorotic stunt virus (SPCSV) and the coat protein (CP) gene sequences of five potyviruses (SPFMV, SPVC, SPVG, SPLV and SPV2). The hsp70 sequences that were obtained provided evidence for the presence of two distinct strains of SPCSV. Analysis of the CP sequences amplified from the samples indicated that all five potyviruses infect Sweet Potato in China, and three different strains of SPFMV and two of SPVG were found.

  • First report of Sweet Potato chlorotic stunt virus infecting Sweet Potato in China.
    Plant Disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Zhengqun Zhang, Desheng Zhang, Yanhong Qin, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5′-ATGRMTACTGRCAAAGTAAACGATG-3′) and SPSP4 (5′-TCAACAGTGAAGACCRGYACCRGTCAA-3′) corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 7...

  • First Report of Sweet Potato chlorotic stunt virus Infecting Sweet Potato in China.
    Plant disease, 2011
    Co-Authors: Qi Qiao, Yuting Tian, Desheng Zhang, Yanhong Qin, Zhenchen Zhang, Yongjiang Wang
    Abstract:

    Sweet Potato chlorotic stunt virus (SPCSV) (genus Crinivirus, family Closteroviridae) is a whitefly-transmitted co-infectant of the Sweet Potato virus disease (SPVD), which can cause severe yield losses in Sweet Potato (Ipomoea batatas (L.) Lam.) (2,3). One isolate was obtained in Guangdong Province in August 2009 from Sweet Potato plants of cv. Guangshu No. 87 exhibiting symptoms typical of SPVD, including stunting, leaf distortion, vein clearing, and chlorosis. Leaf extracts from the symptomatic plants tested positive for SPCSV by nitrocellulose membrane ELISA with antisera specific for SPCSV obtained from the International Potato Center. Total RNA was extracted from young leaves of Sweet Potato and reverse transcription-PCR was performed by using primer pairs SPSP1 (5'-ATGRMTACTGRCAAAGTAAACGATG-3') and SPSP4 (5'-TCAACAGTGAAGACCRGYACCRGTCAA-3') corresponding to the capsid protein (CP) gene of SPCSV. Expected DNA fragments of 774 bp were obtained from symptomatic plants but not from control plants. The 774-bp fragments obtained by amplification were purified and cloned into the PMD19-T vector (TaKaRa, Dalian, China). Recombinant plasmids were then transformed into competent cells of Escherichia coli strain Jm109. Sequencing of the fragments from two individually clones plasmids yielded a nucleotide sequence (GenBank Accession No. HM773432) with 97.4 to 98.7% similarity to the CP gene of East African SPCSV isolates (1). To our knowledge, this is the first report of SPCSV in Sweet Potato fields from China. This virus may threaten Sweet Potato production in China, so cultivars and germplasm should be evaluated for resistance. References: (1) V. Aritua et al. J. Phytopathol. 156:181, 2008. (2) R. W. Njeru et al. Ann. Appl. Biol. 145:71, 2004. (3) G. A. Schaefer and E. R. Terry. Phytopathology 66:642, 1976.

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