The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform
Yijun Zhou - One of the best experts on this subject based on the ideXlab platform.
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Overexpression of OsCIPK30 Enhances Plant Tolerance to Rice Stripe Virus.
Frontiers in microbiology, 2017Co-Authors: Liu Zhiyang, Tong Zhou, Feng Sun, Yijun ZhouAbstract:Rice Stripe Virus (RSV) causes a severe disease in Oryza sativa (Rice) in many Eastern Asian countries. The NS3 protein of RSV is a viral suppressor of RNA silencing, but plant host factors interacting with NS3 have not been reported yet. Here, we present evidence that expression of RSV NS3 in Arabidopsis thaliana causes developmental abnormalities. Through yeast two-hybrid screening and a luciferase complementation imaging assay, we demonstrate that RSV NS3 interacted with OsCIPK30, a CBL (calcineurin B-like proteins)-interaction protein kinase protein. Furthermore, OsCIPK30 was overexpressed to investigate the function of OsCIPK30 in Rice. Our investigation showed that overexpression of OsCIPK30 in Rice could delay the RSV symptoms and show milder RSV symptoms. In addition, the expression of pathogenesis-related genes was increased in OsCIPK30 transgenic Rice. These results suggest that overexpression of OsCIPK30 positively regulates pathogenesis-related genes to enhance the tolerance to RSV in Rice. Our findings provide new insight into the molecular mechanism underlying resistance to RSV disease.
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RNA-seq-based digital gene expression analysis reveals modification of host defense responses by Rice Stripe Virus during disease symptom development in Arabidopsis
Virology journal, 2016Co-Authors: Sun Feng, Fang Peng, Du Linlin, Lan Ying, Zhou Tong, Yongjian Fan, Wenbiao Shen, Yijun ZhouAbstract:Background Virus infection induces and suppresses host gene expression on a global level. Rice Stripe Virus (RSV) is the type species of the genus TenuiVirus and infects Rice and Arabidopsis plants. Microarray-based and next generation sequencing-based transcriptomic approaches have been used to study Rice-RSV interactions. However, our knowledge of the response of Arabidopsis plants to RSV infection is limited, and it requires further investigation to determine the similarities (or differences) in Virus-host interactions between monocot and dicot hosts infected with RSV.
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Assessment of reference gene stability in Rice Stripe Virus and Rice black streaked dwarf Virus infection Rice by quantitative Real-time PCR.
Virology journal, 2015Co-Authors: Fang Peng, Yijun Zhou, Lan Ying, Wenbiao Shen, Feng Sun, Zhou TongAbstract:Background Stably expressed reference gene(s) normalization is important for the understanding of gene expression patterns by quantitative Real-time PCR (RT-qPCR), particularly for Rice Stripe Virus (RSV) and Rice black streaked dwarf Virus (RBSDV) that caused seriously damage on Rice plants in China and Southeast Asia.
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Facilitation of Rice Stripe Virus Accumulation in the Insect Vector by Himetobi P Virus VP1
Viruses, 2015Co-Authors: Xi Wang, Lijuan Sun, Zewen Liu, Yijun ZhouAbstract:The small brown planthopper (SBPH) is the main vector for Rice Stripe Virus (RSV), which causes serious Rice Stripe disease in East Asia. To characterize the Virus-vector interactions, the SBPH cDNA library was screened with RSV ribonucleoprotein (RNP) as bait using a GAL4-based yeast two-hybrid system. The interaction between RSV-RNP and the Himetobi P Virus (HiPV, an insect picorna-like Virus) VP1 protein was identified. The relationships between HiPV and RSV in SBPH were further investigated, and the results showed that the titer of RSV was commonly higher in single insect that exhibited more VP1 expression. After the VP1 gene was repressed by RNA silencing, the accumulation of RSV decreased significantly in the insect, whereas the Virus acquisition ability of SBPH was unaffected, which suggests that HiPV VP1 potentially facilitates the accumulation of RSV in SBPH.
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A simplified method for simultaneous detection of Rice Stripe Virus and Rice black-streaked dwarf Virus in insect vector
Journal of virological methods, 2014Co-Authors: Xi Wang, Yijun ZhouAbstract:Rice Stripe Virus (RSV) and Rice black-streaked dwarf Virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two Viruses in vector, which could facilitate better forecasting and control of the Virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously.
Yongqiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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corrigendum to the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana virology 425 2012 113 121
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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Corrigendum to “The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana” [Virology 425(2012): 113–121 ]
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana
Virology, 2012Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:The Rice Stripe Virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic Virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis in Nicotiana benthamiana, indicating novel functions of the RSV MP. A systematic alanine-scanning mutagenesis study established the region K(122)-D(258) of the pc4 substantially associated with cell-to-cell movement, and mutants by replacement of KGR(122-124), D(135), ED(170-171), ER(201-202), EFE(218-220) or ELD(256-258) with alanine(s) no longer moved cell to cell. However, only one amino acid group KGR(122-124) was linked with long-distance movement. The region D(17)-K(33) was recognized as a crucial domain for leaf necrosis response, and mutagenesis of DD(17-18) or RK(32-33) greatly attenuated necrosis. The overall data suggested manifold roles of the pc4 during the RSV infection in its experimental host N. benthamiana.
Chao Zhang - One of the best experts on this subject based on the ideXlab platform.
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corrigendum to the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana virology 425 2012 113 121
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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Corrigendum to “The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana” [Virology 425(2012): 113–121 ]
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana
Virology, 2012Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:The Rice Stripe Virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic Virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis in Nicotiana benthamiana, indicating novel functions of the RSV MP. A systematic alanine-scanning mutagenesis study established the region K(122)-D(258) of the pc4 substantially associated with cell-to-cell movement, and mutants by replacement of KGR(122-124), D(135), ED(170-171), ER(201-202), EFE(218-220) or ELD(256-258) with alanine(s) no longer moved cell to cell. However, only one amino acid group KGR(122-124) was linked with long-distance movement. The region D(17)-K(33) was recognized as a crucial domain for leaf necrosis response, and mutagenesis of DD(17-18) or RK(32-33) greatly attenuated necrosis. The overall data suggested manifold roles of the pc4 during the RSV infection in its experimental host N. benthamiana.
Shiwei Guo - One of the best experts on this subject based on the ideXlab platform.
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corrigendum to the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana virology 425 2012 113 121
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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Corrigendum to “The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana” [Virology 425(2012): 113–121 ]
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana
Virology, 2012Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:The Rice Stripe Virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic Virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis in Nicotiana benthamiana, indicating novel functions of the RSV MP. A systematic alanine-scanning mutagenesis study established the region K(122)-D(258) of the pc4 substantially associated with cell-to-cell movement, and mutants by replacement of KGR(122-124), D(135), ED(170-171), ER(201-202), EFE(218-220) or ELD(256-258) with alanine(s) no longer moved cell to cell. However, only one amino acid group KGR(122-124) was linked with long-distance movement. The region D(17)-K(33) was recognized as a crucial domain for leaf necrosis response, and mutagenesis of DD(17-18) or RK(32-33) greatly attenuated necrosis. The overall data suggested manifold roles of the pc4 during the RSV infection in its experimental host N. benthamiana.
Shirong Jia - One of the best experts on this subject based on the ideXlab platform.
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corrigendum to the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana virology 425 2012 113 121
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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Corrigendum to “The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana” [Virology 425(2012): 113–121 ]
Virology, 2013Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:Corrigendum to ‘‘The Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana’’ [Virology 425(2012): 113–121 ] Chao Zhang , Xinwu Pei , Zhixing Wang , Shirong Jia , Shiwei Guo , Yongqiang Zhang , Weimin Li a,n a Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 12 South Zhongguancun Street, Beijing 100081, PR China b Institute of Food Crops, Jiangsu High Quality Rice R & D Center, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, PR China
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the Rice Stripe Virus pc4 functions in movement and foliar necrosis expression in nicotiana benthamiana
Virology, 2012Co-Authors: Chao Zhang, Xinwu Pei, Zhixing Wang, Shirong Jia, Shiwei Guo, Yongqiang ZhangAbstract:The Rice Stripe Virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic Virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis in Nicotiana benthamiana, indicating novel functions of the RSV MP. A systematic alanine-scanning mutagenesis study established the region K(122)-D(258) of the pc4 substantially associated with cell-to-cell movement, and mutants by replacement of KGR(122-124), D(135), ED(170-171), ER(201-202), EFE(218-220) or ELD(256-258) with alanine(s) no longer moved cell to cell. However, only one amino acid group KGR(122-124) was linked with long-distance movement. The region D(17)-K(33) was recognized as a crucial domain for leaf necrosis response, and mutagenesis of DD(17-18) or RK(32-33) greatly attenuated necrosis. The overall data suggested manifold roles of the pc4 during the RSV infection in its experimental host N. benthamiana.
