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Li Jiang - One of the best experts on this subject based on the ideXlab platform.
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identification and expression profiles of twenty six glutathione s transferase genes from Rice Weevil sitophilus oryzae coleoptera curculionidae
International Journal of Biological Macromolecules, 2018Co-Authors: Fei Hu, Kan Ye, Xiaofang Tu, Yujie Lu, Kiran Thakur, Li JiangAbstract:Abstract The Rice Weevil, Sitophilus oryzae , is one of the most destructive pests in stored cereals products. In this study, 26 cDNAs encoding glutathione S -transferases (GSTs) were sequenced and characterized in S. oryzae . Phylogenetic analysis displayed the categorization of 26 GSTs into six different cytosolic classes, including two in the delta, twelve in epsilon, three in omega, six in sigma, two in theta, and one in zeta class. RT-qPCR assay illustrated that the relative expression of ten GST genes was significantly higher in adult stages than in larval and pupal developmental stages. Tissue-specific expression analysis revealed that the SoGSTe5 , SoGSTe7 , SoGSTe12 , and SoGSTz1 were up-regulated in the midgut, SoGSTe2 , SoGSTe6 , and SoGSTs2 were up-regulated in the fat body, and three GSTs ( SoGSTd1 , SoGSTd2 and SoGSTe4 ) were up-regulated in Malpighian tubules. RT-qPCR indicated that five GST genes were over expressed after exposure to phosphine at various times and concentrations. The increase in GST gene expressions after phosphine exposure in S. oryzae may lead to an improved tolerance for fumigations and xenobiotics.
L O De Oliveira - One of the best experts on this subject based on the ideXlab platform.
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ancient origin and recent range expansion of the maize Weevil sitophilus zeamais and its genealogical relationship to the Rice Weevil s oryzae
Bulletin of Entomological Research, 2017Co-Authors: Alberto S Correa, Christina C Vinson, Lucas S Braga, R N C Guedes, L O De OliveiraAbstract:: Archeological records attest the early association of Sitophilus with stored cereals from the beginning of agriculture on Asia. The maize Weevil (Sitophilus zeamais) became particularly damaging to maize, a cereal crop domesticated on Mesoamerica. We investigated the late evolutionary history of the maize Weevil to gain insights on its origin, timing of association with maize, and genealogical relationship to the almost morphologically indistinguishable Rice Weevil (Sitophilus oryzae). Two mitochondrial genes (cytochrome oxidase subunit I and cytochrome oxidase subunit II) and the nuclear ribosomal gene region were partially sequenced. Analyses showed that the maize Weevil shared no haplotypes with the Rice Weevil; instead, each species exhibited distinct mitogroups and ribogroups. The two Weevil species likely split about 8.7 million years ago (95% highest posterior density: 4.0-15.0). Microsatellite data analyses sorted the 309 specimens from 15 populations of the maize Weevil into three genotypic groups, which displayed low genetic differentiation and widespread occurrence worldwide. The maize Weevil and the Rice Weevil are each a distinct species; both of which emerged prior to the onset of agriculture. The maize-maize Weevil association took place after maize became widespread as a global crop. The maize Weevil populations lack spatial genetic structure at the regional, continental, and intercontinental scales.
Barry R. Pittendrigh - One of the best experts on this subject based on the ideXlab platform.
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comparison of full mitochondrial genomes for the Rice Weevil sitophilus oryzae and the maize Weevil sitophilus zeamais coleoptera curculionidae
Agri Gene, 2016Co-Authors: Carmen M Valero, Brad S Coates, Adebayo Amos Omoloye, Barry R. PittendrighAbstract:Abstract Complete mitochondrial genome sequences were assembled for the Rice Weevil, Sitophilus oryzae (17,602 bp) and the maize Weevil, S. zeamais (18,105 bp; Coleoptera: Curculionidae: Dryophthorinae), which encode 13 protein coding genes (PCGs), 22 transfer RNAs, and 2 ribosomal RNAs that is typical among Animalia. The A + T-rich control regions of S. oryzae (2818 bp) and S. zeamais (2832 bp) are the longest described to date among curculionoidea (Weevils). Additionally, non-coding intergenic regions have increased in size due to expansion of tandem repeat arrays, but is more pronounced in S. zeamais (606 bp) compared to S. oryzae (109 bp). A total of 69 and 22 substitution mutations were found among reads from S. oryzae and S. zeamais, out of which 68 and 2 were predicted in the PCGs respectively, with a majority in NADH Dehydrogenase subunit I. Phylogenetic analyses of coleopteran insects based upon full mitogenomes PCG sequence supported the existence of Curculionoidea and Tenebrionoidea as a monophyletic group, whereas the Cucujoidea and Elateroidea remain paraphyletic. The mitogenomes of these two Sitophilus species provide insight into short-term evolutionary relationships among curculionid beetles, and provide resources for the potential deciphering of more broad systematic questions in the Order Coleoptera.
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Effects of lectins, CRY1A/CRY1B Bt δ-endotoxin, PAPA, protease and α-amylase inhibitors, on the development of the Rice Weevil, Sitophilus oryzae, using an artificial seed bioassay
Entomologia Experimentalis Et Applicata, 1997Co-Authors: Barry R. Pittendrigh, Joseph E Huesing, Richard E. Shade, Larry L MurdockAbstract:An artificial maize seed bioassay was developed to evaluate potential resistance factors against the Rice Weevil, Sitophilus oryzae. Weevils reared in artificial seeds compared to those reared in whole maize seeds: (i) developed faster, (ii) had similar within-seed developmental mortalities, (iii) were lighter in weight upon emergence and (iv) oviposited the same number of eggs. Using this bioassay we found that E-64, a cysteine protease inhibitor, decreased the number of emerged adults per seed and delayed within-seed developmental time, suggesting that the Rice Weevil utilizes a cysteine protease to digest its dietary protein. Weevils fed inhibitors of trypsin and chymotrypsin, Bowman-Birk and Kunitz inhibitors respectively, developed normally. Para-amino-l-phenylalanine (PAPA), a non-protein amino acid implicated as an insect resistance factor in Vigna vexillata, was lethal at dietary levels of 0.2% (w/w) and higher. An extract from Amaranthus caudatus seeds delayed the developmental time of the Rice Weevil at dietary levels of 0.2% (w/w) and increased mortality at dietary levels of 1.0% (w/w). Several proteins tested, including Griffonia simplicifolia agglutinin II, phytohemagglutinin extract containing common bean α-amylase inhibitor, pokeweed agglutinin, Bacillus thuringiensis CRY1A/CRY1B endotoxin, and an α-amylase inhibitor from wheat, had no effect on the Rice Weevil. The artificial maize seed bioassay was adapted by pelleting the seed for use with an ultrasonic insect feeding monitor to determine the finding activity of Rice Weevils as they developed from egg hatch to pupation.
Bruce C Campbell - One of the best experts on this subject based on the ideXlab platform.
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fumigant toxicity of volatile natural products from korean spices and medicinal plants towards the Rice Weevil sitophilus oryzae l
Pest Management Science, 2001Co-Authors: Wonsik Choi, Byeoung Soo Park, Bruce C CampbellAbstract:: The fumigant toxicity of various volatile constituents of essential oils extracted from sixteen Korean spices and medicinal plants towards the Rice Weevil, Sitophilus oryzae L (Coleoptera: Curculionidae), was determined. The most potent toxicity was found in the essential oil from Mentha arvensis L. var piperascens (LC50 = 45.5 microliters litre-1 air). GC-MS analysis of essential oil from M arvensis showed it to be rich in menthol (63.2%), menthone (13.1%) and limonene (1.5%), followed in abundance by beta-pinene (0.7%), alpha-pinene (0.6%) and linalool (0.2%). Treatment of S oryzae with each of these terpenes showed menthone to be most active (LC50 = 12.7 microliters litre-1 air) followed by linalool (LC50 = 39.2 microliters litre-1 air) and alpha-pinene (LC50 = 54.9 microliters litre-1 air). Studies on inhibition of acetylcholinesterase activity of S oryzae showed menthone to have a nine-fold lower inhibitory effect than menthol, despite menthone being 8.1-fold more toxic than menthol to the Rice Weevil. Different modes of toxicity of these monoterpenes towards S oryzae are discussed.
Fei Hu - One of the best experts on this subject based on the ideXlab platform.
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identification and expression profiles of twenty six glutathione s transferase genes from Rice Weevil sitophilus oryzae coleoptera curculionidae
International Journal of Biological Macromolecules, 2018Co-Authors: Fei Hu, Kan Ye, Xiaofang Tu, Yujie Lu, Kiran Thakur, Li JiangAbstract:Abstract The Rice Weevil, Sitophilus oryzae , is one of the most destructive pests in stored cereals products. In this study, 26 cDNAs encoding glutathione S -transferases (GSTs) were sequenced and characterized in S. oryzae . Phylogenetic analysis displayed the categorization of 26 GSTs into six different cytosolic classes, including two in the delta, twelve in epsilon, three in omega, six in sigma, two in theta, and one in zeta class. RT-qPCR assay illustrated that the relative expression of ten GST genes was significantly higher in adult stages than in larval and pupal developmental stages. Tissue-specific expression analysis revealed that the SoGSTe5 , SoGSTe7 , SoGSTe12 , and SoGSTz1 were up-regulated in the midgut, SoGSTe2 , SoGSTe6 , and SoGSTs2 were up-regulated in the fat body, and three GSTs ( SoGSTd1 , SoGSTd2 and SoGSTe4 ) were up-regulated in Malpighian tubules. RT-qPCR indicated that five GST genes were over expressed after exposure to phosphine at various times and concentrations. The increase in GST gene expressions after phosphine exposure in S. oryzae may lead to an improved tolerance for fumigations and xenobiotics.
