The Experts below are selected from a list of 51 Experts worldwide ranked by ideXlab platform
Fugen Wu - One of the best experts on this subject based on the ideXlab platform.
-
permeabilization tolerant plasma membrane imaging reagent based on amine Rich Glycol chitosan derivatives
ACS Biomaterials Science & Engineering, 2017Co-Authors: Hongyin Wang, Yan Hong Li, Zhan Chen, Fugen WuAbstract:Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene Glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-Rich Glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane prote...
Hongyin Wang - One of the best experts on this subject based on the ideXlab platform.
-
permeabilization tolerant plasma membrane imaging reagent based on amine Rich Glycol chitosan derivatives
ACS Biomaterials Science & Engineering, 2017Co-Authors: Hongyin Wang, Yan Hong Li, Zhan Chen, Fugen WuAbstract:Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene Glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-Rich Glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane prote...
-
Permeabilization-Tolerant Plasma Membrane Imaging Reagent Based on Amine-Rich Glycol Chitosan Derivatives
2017Co-Authors: Hongyin Wang, Jie Sun, Liu-yuan Xia, Zhan ChenAbstract:Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene Glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-Rich Glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane proteins/lipids, thus preventing the detachment of fluorophores from the cell surface after permeabilization. Besides, the large molecular weight effect of the imaging reagent may also account for its antipermeabilization property. Furthermore, by combining immunofluorescence staining with the plasma membrane labeling by GC-PEG Chol-FITC, we simultaneously imaged the plasma membrane and cytoskeletons, and clearly observed metaphase cells and binucleated cells. The concept of using amine-Rich polymeric dyes for plasma membrane imaging will inspire the development of more permeabilization-resistant membrane labeling dyes with better performance, which can realize simultaneous membrane and intracellular protein imaging and facilitate the future studies of membrane–intracellular protein interactions
Zhan Chen - One of the best experts on this subject based on the ideXlab platform.
-
permeabilization tolerant plasma membrane imaging reagent based on amine Rich Glycol chitosan derivatives
ACS Biomaterials Science & Engineering, 2017Co-Authors: Hongyin Wang, Yan Hong Li, Zhan Chen, Fugen WuAbstract:Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene Glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-Rich Glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane prote...
-
Permeabilization-Tolerant Plasma Membrane Imaging Reagent Based on Amine-Rich Glycol Chitosan Derivatives
2017Co-Authors: Hongyin Wang, Jie Sun, Liu-yuan Xia, Zhan ChenAbstract:Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene Glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-Rich Glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane proteins/lipids, thus preventing the detachment of fluorophores from the cell surface after permeabilization. Besides, the large molecular weight effect of the imaging reagent may also account for its antipermeabilization property. Furthermore, by combining immunofluorescence staining with the plasma membrane labeling by GC-PEG Chol-FITC, we simultaneously imaged the plasma membrane and cytoskeletons, and clearly observed metaphase cells and binucleated cells. The concept of using amine-Rich polymeric dyes for plasma membrane imaging will inspire the development of more permeabilization-resistant membrane labeling dyes with better performance, which can realize simultaneous membrane and intracellular protein imaging and facilitate the future studies of membrane–intracellular protein interactions
Yan Hong Li - One of the best experts on this subject based on the ideXlab platform.
-
permeabilization tolerant plasma membrane imaging reagent based on amine Rich Glycol chitosan derivatives
ACS Biomaterials Science & Engineering, 2017Co-Authors: Hongyin Wang, Yan Hong Li, Zhan Chen, Fugen WuAbstract:Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene Glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-Rich Glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane prote...
Mohammed Rashnur Rahman - One of the best experts on this subject based on the ideXlab platform.
-
Plant based on the operating condition of Titas Gas Field Location #A
2015Co-Authors: Mohammed Rashnur Rahman, N. M. Aftabul, Alam Bhuiya, Md. Rasel MiahAbstract:Copyright © 2014 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT: Natural gas processing is a complex industrial process designed to reduce impurities from raw natural gas by separation process to produce gas which is known as pipeline quality dry natural gas. In Titas Gas Field (Location #A), natural gas process plant is mainly designed to separate the water from the raw gas and make the gas transmittable to the consumer. Natural gas dehydration for Titas Gas Field mainly includes the separation of water from gas by Glycol Dehydration Process. Besides, a little amount of condensate is also separated during the dehydration process. Condensate of Titas Gas Field mainly contents hydrocarbon of C3-C4. As the absorbent, TEG (Tri-Ethylene Glycol) is the most preferable to use. In this paper, we represent the design of the equipment using in the Glycol dehydration plant of Titas Gas Field, Location #A. Equipment sizing means calculating optimum height, diameter, number of tray, capacity, circulation rate etc. of each equipment. Calculation of height, diameter, number of tray capacity, circulating rate etc. of the equipment by using various tables, figures, charts, methods. In this paper we designed Inlet 3-phase separator, Glycol contactor tower, Lean-Rich Glycol heat exchanger, Glycol circulation pump, Glycol flash separator (3-phase), Glycol regenerator (Re-boiler), Stripping still. It has to be mentioned that this is a theoretical design of the equipment for a Glycol dehydration plant based on the operatin
-
Theoretical Sizing and Design of The Equipment of a 40 MMSCFD Natural Gas Processing Plant based on the operating condition of Titas Gas Field Location #A
International Journal of Innovation and Applied Studies, 2014Co-Authors: Mohammed Rashnur Rahman, N.m. Aftabul Alam Bhuiya, Rasel MiahAbstract:Natural gas processing is a complex industrial process designed to reduce impurities from raw natural gas by separation process to produce gas which is known as pipeline quality dry natural gas. In Titas Gas Field (Location #A), natural gas process plant is mainly designed to separate the water from the raw gas and make the gas transmittable to the consumer. Natural gas dehydration for Titas Gas Field mainly includes the separation of water from gas by Glycol Dehydration Process. Besides, a little amount of condensate is also separated during the dehydration process. Condensate of Titas Gas Field mainly contents hydrocarbon of C3-C4. As the absorbent, TEG (Tri-Ethylene Glycol) is the most preferable to use. In this paper, we represent the design of the equipment using in the Glycol dehydration plant of Titas Gas Field, Location #A. Equipment sizing means calculating optimum height, diameter, number of tray, capacity, circulation rate etc. of each equipment. Calculation of height, diameter, number of tray capacity, circulating rate etc. of the equipment by using various tables, figures, charts, methods. In this paper we designed Inlet 3-phase separator, Glycol contactor tower, Lean-Rich Glycol heat exchanger, Glycol circulation pump, Glycol flash separator (3-phase), Glycol regenerator (Re-boiler), Stripping still. It has to be mentioned that this is a theoretical design of the equipment for a Glycol dehydration plant based on the operating condition of Titas Gas Field, Location #A which can be more efficient than the operating equipment.
