The Experts below are selected from a list of 29967 Experts worldwide ranked by ideXlab platform
Vijay Lyall - One of the best experts on this subject based on the ideXlab platform.
-
In situ hybridization of Chrnb4 digoxigenin labeled anti-sense RNA Probe in WT mouse CV taste bud cells.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:Shows merged images of a WT mouse FF taste papilla section labelled with Alexa Fluor® 488 (FITC) and the DIC image. The anti-sense (AS) riboProbe Chrnb4 labeled a subset of CV taste bud cells (A) and FF taste bud cells (B). No significant fluorescence was observed when the sense Probe (S) was used in a FF taste papilla section (C).
-
In situ hybridization of Chrnb2 digoxigenin labeled anti-sense RNA Probe in FF and CV taste bud cells.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:(FF) Shows images of a WT mouse FF taste papilla section labelled with Alexa Fluor® 488 (FITC), DAPI, the merged image of DAPI and FITC, and the DIC image. The anti-sense (AS) Chrnb2 riboProbe labeled a subset of FF taste bud cells. No significant fluorescence was observed when the sense Probe (S) was used. (CV) Shows low magnification images of a WT mouse CV taste papilla section labelled with Alexa Fluor® 488 (FITC), DIC image, and the merged image of FITC and the DIC image. The anti-sense (AS) Chrnb2 riboProbe labeled a subset of WT mouse CV taste bud cells. No significant fluorescence was observed when the sense (S) Probe was used. Bar = 10 μm.
-
In situ hybridization of Trpm5 digoxigenin labeled anti-sense RNA Probe in rat CV taste papillae sections.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:Shows low magnification images of rat CV papilla sections labelled with Alexa Fluor® 488 (FITC), DAPI, the merged images of FITC and DAPI, and the DIC image. The anti-sense (AS) Trpm5 riboProbe labeled a subset of rat CV taste bud cells (AS). No significant fluorescence was observed when the sense (S) Probe was used. Bar = 10 μm.
-
In situ hybridization of ChRNA7 digoxigenin labeled anti-sense RNA Probe in rat CV taste papillae sections.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:Shows images of rat CV papilla sections labelled with Alexa Fluor® 488 (FITC), DAPI, the merged images of FITC and DAPI, and the DIC image. The anti-sense (AS) ChRNA7 riboProbe labeled a subset of rat CV taste bud cells (AS). No significant fluorescence was observed when the sense (S) Probe was used. Bar = 10 μm.
Ethan Bier - One of the best experts on this subject based on the ideXlab platform.
-
double label in situ hybridization using biotin and digoxigenin tagged RNA Probes
BioTechniques, 1994Co-Authors: Jason W Oneill, Ethan BierAbstract:We describe a double-label in situ hybridization protocol based on the optimized synthesis of biotin-labeled RNA Probes. Biotin-labeled Probes are used in conjunction with digoxigenin-labeled Probes to simultaneously visualize two different transcripts. One transcript is hybridized with a biotin-labeled RNA Probe and visualized as a brown peroxidase reaction product, and the other transcript is hybridized with a digoxigenin-labeled RNA Probe and visualized as a blue alkaline phosphatase reaction product. We present several examples in which this double-labeling method has proven useful in determining the spatial and temporal relationships between various transcripts expressed during Drosophila embryogenesis, indicating that this method should be of general use in establishing the relationship of two independent transcription patterns.
Chunyan Yeung - One of the best experts on this subject based on the ideXlab platform.
-
salmonella detection using 16s ribosomal dna RNA Probe gold nanoparticles and lateral flow immunoassay
Food Chemistry, 2013Co-Authors: Chunyan Yeung, Pohao ChenAbstract:Abstract An ultrasensitive, simple, and fast lateral flow immunoassay for Salmonella detection using gold nanoparticles conjugated with a DNA Probe, which is complementary to the 16S ribosomal RNA and DNA of Salmonella , has been developed. The detection limit is 5 fmol for the synthetic single-stranded DNA. For the Salmonella cultured samples, the nucleic acids from 10 7 bacteria were rapidly detected in 30 min. After silver enhancement, the detection limit was as low as 10 4 cells which is lower than 10 5 bacteria cells, the human infective dose of food-borne Salmonella . Furthermore, the Probes used in this study are specific to Salmonella compared to several other Enterobacteriaceae . This approach would be a useful tool for microbial detection regarding food safety or clinical diagnosis. It is also suitable for large-scale screening in developing countries because it is low-cost, sensitive, specific and convenient.
Jie Qian - One of the best experts on this subject based on the ideXlab platform.
-
In situ hybridization of Chrnb4 digoxigenin labeled anti-sense RNA Probe in WT mouse CV taste bud cells.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:Shows merged images of a WT mouse FF taste papilla section labelled with Alexa Fluor® 488 (FITC) and the DIC image. The anti-sense (AS) riboProbe Chrnb4 labeled a subset of CV taste bud cells (A) and FF taste bud cells (B). No significant fluorescence was observed when the sense Probe (S) was used in a FF taste papilla section (C).
-
In situ hybridization of Chrnb2 digoxigenin labeled anti-sense RNA Probe in FF and CV taste bud cells.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:(FF) Shows images of a WT mouse FF taste papilla section labelled with Alexa Fluor® 488 (FITC), DAPI, the merged image of DAPI and FITC, and the DIC image. The anti-sense (AS) Chrnb2 riboProbe labeled a subset of FF taste bud cells. No significant fluorescence was observed when the sense Probe (S) was used. (CV) Shows low magnification images of a WT mouse CV taste papilla section labelled with Alexa Fluor® 488 (FITC), DIC image, and the merged image of FITC and the DIC image. The anti-sense (AS) Chrnb2 riboProbe labeled a subset of WT mouse CV taste bud cells. No significant fluorescence was observed when the sense (S) Probe was used. Bar = 10 μm.
-
In situ hybridization of Trpm5 digoxigenin labeled anti-sense RNA Probe in rat CV taste papillae sections.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:Shows low magnification images of rat CV papilla sections labelled with Alexa Fluor® 488 (FITC), DAPI, the merged images of FITC and DAPI, and the DIC image. The anti-sense (AS) Trpm5 riboProbe labeled a subset of rat CV taste bud cells (AS). No significant fluorescence was observed when the sense (S) Probe was used. Bar = 10 μm.
-
In situ hybridization of ChRNA7 digoxigenin labeled anti-sense RNA Probe in rat CV taste papillae sections.
2018Co-Authors: Jie Qian, Shobha Mummalaneni, John R. Grider, Imad M. Damaj, Vijay LyallAbstract:Shows images of rat CV papilla sections labelled with Alexa Fluor® 488 (FITC), DAPI, the merged images of FITC and DAPI, and the DIC image. The anti-sense (AS) ChRNA7 riboProbe labeled a subset of rat CV taste bud cells (AS). No significant fluorescence was observed when the sense (S) Probe was used. Bar = 10 μm.
Pohao Chen - One of the best experts on this subject based on the ideXlab platform.
-
salmonella detection using 16s ribosomal dna RNA Probe gold nanoparticles and lateral flow immunoassay
Food Chemistry, 2013Co-Authors: Chunyan Yeung, Pohao ChenAbstract:Abstract An ultrasensitive, simple, and fast lateral flow immunoassay for Salmonella detection using gold nanoparticles conjugated with a DNA Probe, which is complementary to the 16S ribosomal RNA and DNA of Salmonella , has been developed. The detection limit is 5 fmol for the synthetic single-stranded DNA. For the Salmonella cultured samples, the nucleic acids from 10 7 bacteria were rapidly detected in 30 min. After silver enhancement, the detection limit was as low as 10 4 cells which is lower than 10 5 bacteria cells, the human infective dose of food-borne Salmonella . Furthermore, the Probes used in this study are specific to Salmonella compared to several other Enterobacteriaceae . This approach would be a useful tool for microbial detection regarding food safety or clinical diagnosis. It is also suitable for large-scale screening in developing countries because it is low-cost, sensitive, specific and convenient.
