The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Mihaela Zavolan - One of the best experts on this subject based on the ideXlab platform.
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Conserved regulation of RNA Processing in somatic cell reprogramming
BMC Genomics, 2019Co-Authors: Alexander Kanitz, Afzal Pasha Syed, Keisuke Kaji, Mihaela ZavolanAbstract:BackgroundAlong with the reorganization of epigenetic and transcriptional networks, somatic cell reprogramming brings about numerous changes at the level of RNA Processing. These include the expression of specific transcript isoforms and 3’ untranslated regions. A number of studies have uncovered RNA Processing factors that modulate the efficiency of the reprogramming process. However, a comprehensive evaluation of the involvement of RNA Processing factors in the reprogramming of somatic mammalian cells is lacking.ResultsHere, we used data from a large number of studies carried out in three mammalian species, mouse, chimpanzee and human, to uncover consistent changes in gene expression upon reprogramming of somatic cells. We found that a core set of nine splicing factors have consistent changes across the majority of data sets in all three species. Most striking among these are ESRP1 and ESRP2, which accelerate and enhance the efficiency of somatic cell reprogramming by promoting isoform expression changes associated with mesenchymal-to-epithelial transition. We further identify genes and processes in which splicing changes are observed in both human and mouse.ConclusionsOur results provide a general resource for gene expression and splicing changes that take place during somatic cell reprogramming. Furthermore, they support the concept that splicing factors with evolutionarily conserved, cell type-specific expression can modulate the efficiency of the process by reinforcing intermediate states resembling the cell types in which these factors are normally expressed.
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Conserved regulation of RNA Processing in somatic cell reprogramming.
BMC Genomics, 2019Co-Authors: Alexander Kanitz, Afzal Pasha Syed, Keisuke Kaji, Mihaela ZavolanAbstract:Along with the reorganization of epigenetic and transcriptional networks, somatic cell reprogramming brings about numerous changes at the level of RNA Processing. These include the expression of specific transcript isoforms and 3’ untranslated regions. A number of studies have uncovered RNA Processing factors that modulate the efficiency of the reprogramming process. However, a comprehensive evaluation of the involvement of RNA Processing factors in the reprogramming of somatic mammalian cells is lacking. Here, we used data from a large number of studies carried out in three mammalian species, mouse, chimpanzee and human, to uncover consistent changes in gene expression upon reprogramming of somatic cells. We found that a core set of nine splicing factors have consistent changes across the majority of data sets in all three species. Most striking among these are ESRP1 and ESRP2, which accelerate and enhance the efficiency of somatic cell reprogramming by promoting isoform expression changes associated with mesenchymal-to-epithelial transition. We further identify genes and processes in which splicing changes are observed in both human and mouse. Our results provide a general resource for gene expression and splicing changes that take place during somatic cell reprogramming. Furthermore, they support the concept that splicing factors with evolutionarily conserved, cell type-specific expression can modulate the efficiency of the process by reinforcing intermediate states resembling the cell types in which these factors are normally expressed.
Nick J Proudfoot - One of the best experts on this subject based on the ideXlab platform.
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Connecting transcription to messenger RNA Processing
Trends in Biochemical Sciences, 2000Co-Authors: Nick J ProudfootAbstract:Abstract The production of messenger RNA by gene transcription requires at least three RNA-Processing mechanisms: capping, splicing and polyadenylation. All three reactions occur in intimate association with the elongating polymerase complex through the C terminus of the largest subunit of RNA polymerase II. The Processing of mRNA is therefore orchestrated to act on the nascent RNA as soon as it emerges from the polymerase complex.
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Connecting transcription to messenger RNA Processing
Trends in Biochemical Sciences, 2000Co-Authors: Nick J ProudfootAbstract:The production of messenger RNA by gene transcription requires at least three RNA-Processing mechanisms: capping, splicing and polyadenylation. All three reactions occur in intimate association with the elongating polymerase complex through the C terminus of the largest subunit of RNA polymerase II. The Processing of mRNA is therefore orchestrated to act on the nascent RNA as soon as it emerges from the polymerase complex. Copyright (C) 2000 Elsevier Science Ltd.
Noora Kotaja - One of the best experts on this subject based on the ideXlab platform.
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The chromatoid body: a germ-cell-specific RNA-Processing centre
Nature Reviews Molecular Cell Biology, 2007Co-Authors: Noora Kotaja, Paolo Sassone-corsiAbstract:The chromatoid body, a unique cloud-like structure of male germ cells, moves dynamically in the cytoplasm of haploid spermatids, but its function has remained elusive for decades. Recent findings indicate that microRNA and RNA-decay pathways converge to the chromatoid body. This highly specialized structure might function as an intracellular focal domain that organizes and controls RNA Processing in male germ cells. The chromatoid body, a unique cloud-like structure of male germ cells, has puzzled scientists for years. Recent findings indicate that microRNA and RNA-decay pathways converge at the chromatoid body, which might function as a germ-cell-specific RNA-Processing centre.
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the chromatoid body a germ cell specific RNA Processing centre
Nature Reviews Molecular Cell Biology, 2007Co-Authors: Noora Kotaja, Paolo SassonecorsiAbstract:The chromatoid body, a unique cloud-like structure of male germ cells, has puzzled scientists for years. Recent findings indicate that microRNA and RNA-decay pathways converge at the chromatoid body, which might function as a germ-cell-specific RNA-Processing centre.
Alexander Kanitz - One of the best experts on this subject based on the ideXlab platform.
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Conserved regulation of RNA Processing in somatic cell reprogramming
BMC Genomics, 2019Co-Authors: Alexander Kanitz, Afzal Pasha Syed, Keisuke Kaji, Mihaela ZavolanAbstract:BackgroundAlong with the reorganization of epigenetic and transcriptional networks, somatic cell reprogramming brings about numerous changes at the level of RNA Processing. These include the expression of specific transcript isoforms and 3’ untranslated regions. A number of studies have uncovered RNA Processing factors that modulate the efficiency of the reprogramming process. However, a comprehensive evaluation of the involvement of RNA Processing factors in the reprogramming of somatic mammalian cells is lacking.ResultsHere, we used data from a large number of studies carried out in three mammalian species, mouse, chimpanzee and human, to uncover consistent changes in gene expression upon reprogramming of somatic cells. We found that a core set of nine splicing factors have consistent changes across the majority of data sets in all three species. Most striking among these are ESRP1 and ESRP2, which accelerate and enhance the efficiency of somatic cell reprogramming by promoting isoform expression changes associated with mesenchymal-to-epithelial transition. We further identify genes and processes in which splicing changes are observed in both human and mouse.ConclusionsOur results provide a general resource for gene expression and splicing changes that take place during somatic cell reprogramming. Furthermore, they support the concept that splicing factors with evolutionarily conserved, cell type-specific expression can modulate the efficiency of the process by reinforcing intermediate states resembling the cell types in which these factors are normally expressed.
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Conserved regulation of RNA Processing in somatic cell reprogramming.
BMC Genomics, 2019Co-Authors: Alexander Kanitz, Afzal Pasha Syed, Keisuke Kaji, Mihaela ZavolanAbstract:Along with the reorganization of epigenetic and transcriptional networks, somatic cell reprogramming brings about numerous changes at the level of RNA Processing. These include the expression of specific transcript isoforms and 3’ untranslated regions. A number of studies have uncovered RNA Processing factors that modulate the efficiency of the reprogramming process. However, a comprehensive evaluation of the involvement of RNA Processing factors in the reprogramming of somatic mammalian cells is lacking. Here, we used data from a large number of studies carried out in three mammalian species, mouse, chimpanzee and human, to uncover consistent changes in gene expression upon reprogramming of somatic cells. We found that a core set of nine splicing factors have consistent changes across the majority of data sets in all three species. Most striking among these are ESRP1 and ESRP2, which accelerate and enhance the efficiency of somatic cell reprogramming by promoting isoform expression changes associated with mesenchymal-to-epithelial transition. We further identify genes and processes in which splicing changes are observed in both human and mouse. Our results provide a general resource for gene expression and splicing changes that take place during somatic cell reprogramming. Furthermore, they support the concept that splicing factors with evolutionarily conserved, cell type-specific expression can modulate the efficiency of the process by reinforcing intermediate states resembling the cell types in which these factors are normally expressed.
Paolo Sassonecorsi - One of the best experts on this subject based on the ideXlab platform.
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the chromatoid body a germ cell specific RNA Processing centre
Nature Reviews Molecular Cell Biology, 2007Co-Authors: Noora Kotaja, Paolo SassonecorsiAbstract:The chromatoid body, a unique cloud-like structure of male germ cells, has puzzled scientists for years. Recent findings indicate that microRNA and RNA-decay pathways converge at the chromatoid body, which might function as a germ-cell-specific RNA-Processing centre.
