The Experts below are selected from a list of 579 Experts worldwide ranked by ideXlab platform
Jens Zimmer - One of the best experts on this subject based on the ideXlab platform.
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Coupling of organotypic brain slice cultures to silicon-based arrays of electrodes.
Methods, 1999Co-Authors: Henrik Jahnsen, Bjarne Winther Kristensen, P. Thiébaud, Jens Noraberg, Birthe Jakobsen, Marco Bove, Sergio Martinoia, Milena Koudelka-hep, Massimo Grattarola, Jens ZimmerAbstract:Fetal or early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as so-called organotypic brain slice cultures. Here we demonstrate and describe how it is possible to establish such hippocampal rat brain slice cultures on biocompatible silicon-based chips with arrays of electrodes with a histological organization comparable to that of conventional brain slice cultures grown by the Roller Drum technique and on semiporous membranes. Intracellular and extracellular recordings from neurons in the slice cultures show that the electroresponsive properties of the neurons and synaptic circuitry are in accordance with those described for cells in acutely prepared slices of the adult rat hippocampus. Based on the recordings and the possibilities of stimulating the cultured cells through the electrode arrays it is anticipated that the setup eventually will allow long-term studies of defined neuronal networks and provide valuable information on both normal and neurotoxicological and neuropathological conditions.
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Grafting of Dopaminergic Ventral Mesencephalic Slice Cultures to the Striatum of Adult Rats
Experimental neurology, 1994Co-Authors: J.c. Sørensen, K. Østergaard, Jens ZimmerAbstract:Live storage of dopaminergic neurons before intracerebral grafting will allow pregrafting examination and manipulation of the cells, as well as pooling and mixing of cells from several donors. In this study we examined whether mesencephalic dopaminergic neurons, grown in organotypic cultures for 1 week, would survive subsequent grafting to the adult rat striatum. Slices of ventral mesencephalon from neonatal rats were grown by the Roller Drum method for 1 week and then grafted into the striatum of adult rats, with and without preceding 6-hydroxydopamine lesions of their nigrostriatal pathway. Using immunocytochemical staining for tyrosine hydroxylase, cultured dopaminergic neurons were found to survive and to extend fibers into the host striatum when examined 4, 7, 14, 28, and 87 days after grafting. When compared with slices of noncultured mesencephalic tissue from 1-week-old rats the slice culture period did not significantly reduce the number of surviving tyrosine hydroxylase positive neurons. From this we conclude that slice cultures can be used for transient storage of dopaminergic donor tissue before intracerebral grafting. The surviving tyrosine hydroxylase positive neurons in control grafts from 1-week-old rats, furthermore, extends the time frame of possible donor ages used for grafting.
Eric Herlenius - One of the best experts on this subject based on the ideXlab platform.
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Current Protocols in Stem Cell Biology - Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures.
Current protocols in stem cell biology, 2017Co-Authors: David Forsberg, Charoensri Thonabulsombat, Petri Olivius, Johan Jäderstad, Linda Maria Jäderstad, Eric HerleniusAbstract:Re-formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell–mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option for in vitro studies is to examine the integration of exogenous stem cells into an existing active neural network in ex vivo organotypic cultures. Organotypic cultures leave the structural integrity essentially intact while still allowing the microenvironment to be carefully controlled. This allows detailed studies over time of cellular responses and cell-cell interactions, which are not readily performed in vivo. This unit describes procedures for using organotypic slice cultures as ex vivo model systems for studying neural stem cell and embryonic stem cell engraftment and communication with CNS host tissue. © 2017 by John Wiley & Sons, Inc. Keywords: brainstem; engraftment; integration; interaction; neuroprotection; neural stem cells; organotypic culture; Roller Drum; striatum; Stoppini; transplantation
Petri Olivius - One of the best experts on this subject based on the ideXlab platform.
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Current Protocols in Stem Cell Biology - Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures.
Current protocols in stem cell biology, 2017Co-Authors: David Forsberg, Charoensri Thonabulsombat, Petri Olivius, Johan Jäderstad, Linda Maria Jäderstad, Eric HerleniusAbstract:Re-formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell–mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option for in vitro studies is to examine the integration of exogenous stem cells into an existing active neural network in ex vivo organotypic cultures. Organotypic cultures leave the structural integrity essentially intact while still allowing the microenvironment to be carefully controlled. This allows detailed studies over time of cellular responses and cell-cell interactions, which are not readily performed in vivo. This unit describes procedures for using organotypic slice cultures as ex vivo model systems for studying neural stem cell and embryonic stem cell engraftment and communication with CNS host tissue. © 2017 by John Wiley & Sons, Inc. Keywords: brainstem; engraftment; integration; interaction; neuroprotection; neural stem cells; organotypic culture; Roller Drum; striatum; Stoppini; transplantation
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Implanted embryonic sensory neurons project axons toward adult auditory brainstem neurons in Roller Drum and Stoppini co-cultures.
Brain research, 2007Co-Authors: Charoensri Thonabulsombat, Saga Johansson, Christian Spenger, Mats Ulfendahl, Petri OliviusAbstract:Previously we have shown in vivo the survival, migration and integration of embryonic dorsal root ganglion (DRG) neurons that were grafted into the inner ear and peripheral auditory nervous system. In order to evaluate relevant factors determining integration of sensory neurons further into the central auditory nervous system, complementary in vitro techniques are necessary. The advantages of in vitro systems are that a large number of factors including various grafts and different conditions can be efficiently examined for. Hence, we co-cultured 300 microm thick postnatal rat brainstem slices containing the cochlear nucleus including the central part of the 8th cranial nerve with mouse embryonic DRG neurons. The organotypic co-cultures were either grown on coverslips using the Roller Drum method described by Gahwiler or on membranes according to the interface method described by Stoppini. Neurons in the cochlear nucleus were labeled with DiI. The results demonstrate that (1) brainstem slices survive for up to 5 weeks in culture, and that (2) co-cultures of embryonic sensory neurons and brainstem show a high degree of neuronal survival, and that (3) survival and axonal outgrowth from the implanted embryonic neurons are dependent on the presence of the brainstem slice rather than on exogenous NGF and that (4) implanted embryonic neurons send axons toward neurons in the cochlear nucleus.
Sheryl L.g. Johnston - One of the best experts on this subject based on the ideXlab platform.
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Comparison of human rhabdomyosarcoma, HEp-2, and human foreskin fibroblast cells for the isolation of herpes simplex virus from clinical specimens.
Diagnostic microbiology and infectious disease, 1991Co-Authors: Sheryl L.g. JohnstonAbstract:Abstract The performance of locally produced human rhabdomyosarcoma (RD) cells was shown to be superior to human foreskin fibroblast (SF) and HEp-2 cells for the isolation of the herpes simplex virus (HSV) from clinical specimens. A comparison of sensitivity, clarity of cytophathic effect, and ease of growing the cells for the isolation of HSV was made on these cell lines. A total of 1351 fresh clinical specimens, submitted for the isolation of HSV, were inoculated into RD, HEp-2, and SF cell culture tubes. The cultures were incubated for 7 days at 35°C on a Roller Drum and observed daily for cytophathic effect (CPE). Of these specimens, 318 (23.5%) were positive for HSV. In 287 (90%) of these positive specimens, the RD cell line was the first, either alone or simultaneously with another cell line, to display typical HSV CPE. This CPE was clearly recognized by the appearance of clusters of rounded cells and associated multinucleated giant cells. In 241 (76%) of the positive specimens, the RD cell line displayed CPE on the first or second day of incubation. These results demonstrate that the RD cell line was more rapid than the SF and HEp-2 cell lines for the detection of HSV and suggests that RD cells should be included in a tissue culture program designed for the detection of HSV.
Charoensri Thonabulsombat - One of the best experts on this subject based on the ideXlab platform.
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Current Protocols in Stem Cell Biology - Functional Stem Cell Integration into Neural Networks Assessed by Organotypic Slice Cultures.
Current protocols in stem cell biology, 2017Co-Authors: David Forsberg, Charoensri Thonabulsombat, Petri Olivius, Johan Jäderstad, Linda Maria Jäderstad, Eric HerleniusAbstract:Re-formation or preservation of functional, electrically active neural networks has been proffered as one of the goals of stem cell–mediated neural therapeutics. A primary issue for a cell therapy approach is the formation of functional contacts between the implanted cells and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option for in vitro studies is to examine the integration of exogenous stem cells into an existing active neural network in ex vivo organotypic cultures. Organotypic cultures leave the structural integrity essentially intact while still allowing the microenvironment to be carefully controlled. This allows detailed studies over time of cellular responses and cell-cell interactions, which are not readily performed in vivo. This unit describes procedures for using organotypic slice cultures as ex vivo model systems for studying neural stem cell and embryonic stem cell engraftment and communication with CNS host tissue. © 2017 by John Wiley & Sons, Inc. Keywords: brainstem; engraftment; integration; interaction; neuroprotection; neural stem cells; organotypic culture; Roller Drum; striatum; Stoppini; transplantation
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Implanted embryonic sensory neurons project axons toward adult auditory brainstem neurons in Roller Drum and Stoppini co-cultures.
Brain research, 2007Co-Authors: Charoensri Thonabulsombat, Saga Johansson, Christian Spenger, Mats Ulfendahl, Petri OliviusAbstract:Previously we have shown in vivo the survival, migration and integration of embryonic dorsal root ganglion (DRG) neurons that were grafted into the inner ear and peripheral auditory nervous system. In order to evaluate relevant factors determining integration of sensory neurons further into the central auditory nervous system, complementary in vitro techniques are necessary. The advantages of in vitro systems are that a large number of factors including various grafts and different conditions can be efficiently examined for. Hence, we co-cultured 300 microm thick postnatal rat brainstem slices containing the cochlear nucleus including the central part of the 8th cranial nerve with mouse embryonic DRG neurons. The organotypic co-cultures were either grown on coverslips using the Roller Drum method described by Gahwiler or on membranes according to the interface method described by Stoppini. Neurons in the cochlear nucleus were labeled with DiI. The results demonstrate that (1) brainstem slices survive for up to 5 weeks in culture, and that (2) co-cultures of embryonic sensory neurons and brainstem show a high degree of neuronal survival, and that (3) survival and axonal outgrowth from the implanted embryonic neurons are dependent on the presence of the brainstem slice rather than on exogenous NGF and that (4) implanted embryonic neurons send axons toward neurons in the cochlear nucleus.
