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Søren Brøgger Christensen - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized rose hip powder Rosa Canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
Lasse Saaby - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized rose hip powder Rosa Canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
Ester Speroni - One of the best experts on this subject based on the ideXlab platform.
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in vivo anti inflammatory effect of Rosa Canina l extract
Journal of Ethnopharmacology, 2011Co-Authors: Francesca Lattanzio, Emanuela Greco, Donatella Carretta, Rinaldo Cervellati, Paolo Govoni, Ester SperoniAbstract:Abstract Ethnopharmacological relevance Rosa Canina L. is a medicinal plant largely used in traditional folk medicine. Several compounds from rose hip extracts were reported to display in vitro anti-inflammatory activities. Aim of the study The in vivo effects of Rosa Canina extracts are still poorly investigated. In the present study the anti-inflammatory and the gastroprotective effects of a hydroalcoholic crude extract of Rosa Canina fruits were tested in rat. Materials and methods The anti-inflammatory activity of the extract was tested on the carrageenin-induced rat paw edema assay. The gastroprotective effect was investigated on the ethanol-induced gastric damage model. The in vitro antioxidant activity of this extract was also quantified using the Briggs–Rauscher oscillating reaction, the Trolox Equivalent Antioxidant Capacity method, and the Total Phenolic Content. Results Data show that the Rosa Canina extract inhibits the development of carrageenin-induced edema; the anti-inflammatory power is similar to that of indomethacin. The antiedema effect was more significant using a higher dose of the extract. The total score expressing gastric damage was lower in Rosa Canina pre-treated stomachs with respect to unpre-treated ones, although the antiulcerogenic effectiveness was not statistically significant. The antiulcerogenic effectiveness was not statistically detectable, even if the total score expressing gastric damage was lower in Rosa Canina stomachs from pre-treated rats with respect to unpre-treated ones. Chemical analysis revealed that the extract owns a good antioxidant activity that may also contribute to the anti-inflammatory effects observed in vivo. Conclusions Altogether, the present data demonstrate the anti-inflammatory property of Rosa Canina suggesting its potential role as adjuvant therapeutic tool for the management of inflammatory-related diseases.
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In vivo anti-inflammatory effect of Rosa Canina L. extract
Journal of ethnopharmacology, 2011Co-Authors: Francesca Lattanzio, Emanuela Greco, Donatella Carretta, Rinaldo Cervellati, Paolo Govoni, Ester SperoniAbstract:Rosa Canina L. is a medicinal plant largely used in traditional folk medicine. Several compounds from rose hip extracts were reported to display in vitro anti-inflammatory activities. The in vivo effects of Rosa Canina extracts are still poorly investigated. In the present study the anti-inflammatory and the gastroprotective effects of a hydroalcoholic crude extract of Rosa Canina fruits were tested in rat. The anti-inflammatory activity of the extract was tested on the carrageenin-induced rat paw edema assay. The gastroprotective effect was investigated on the ethanol-induced gastric damage model. The in vitro antioxidant activity of this extract was also quantified using the Briggs-Rauscher oscillating reaction, the Trolox Equivalent Antioxidant Capacity method, and the Total Phenolic Content. Data show that the Rosa Canina extract inhibits the development of carrageenin-induced edema; the anti-inflammatory power is similar to that of indomethacin. The antiedema effect was more significant using a higher dose of the extract. The total score expressing gastric damage was lower in Rosa Canina pre-treated stomachs with respect to unpre-treated ones, although the antiulcerogenic effectiveness was not statistically significant. The antiulcerogenic effectiveness was not statistically detectable, even if the total score expressing gastric damage was lower in Rosa Canina stomachs from pre-treated rats with respect to unpre-treated ones. Chemical analysis revealed that the extract owns a good antioxidant activity that may also contribute to the anti-inflammatory effects observed in vivo. Altogether, the present data demonstrate the anti-inflammatory property of Rosa Canina suggesting its potential role as adjuvant therapeutic tool for the management of inflammatory-related diseases. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Anna K. Jäger - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized rose hip powder Rosa Canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
Lise Moesby - One of the best experts on this subject based on the ideXlab platform.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.).
Phytotherapy research : PTR, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC(50) 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
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isolation of immunomodulatory triterpene acids from a standardized rose hip powder Rosa Canina l
Phytotherapy Research, 2011Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previously published systematic review and a metaanalysis have concluded that the consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and mainly to involve infiltration of the synovial membrane by macrophages. Therefore, the immunomodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µm). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids. Copyright © 2010 John Wiley & Sons, Ltd.
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Isolation of immunomodulatory triterpene acids from a standardized rose hip powder (Rosa Canina L.)
Phytotherapy Research, 2010Co-Authors: Lasse Saaby, Anna K. Jäger, Lise Moesby, Erik Wind Hansen, Søren Brøgger ChristensenAbstract:A previous published systematic review and a metaanalysis have concluded that consumption of standardized rose hip powder (Rosa Canina L.) can reduce pain in osteoarthritis patients. Synovial inflammation has been suggested to play an important role in the pathogenesis of osteoarthritis and to mainly involve infiltration of the synovial membrane by macrophages. Therefore, the immunmodulatory effect of standardized rose hip powder of Rosa Canina L. was investigated and active principles isolated using the Mono Mac 6 cell line as a model for human macrophages. Treatment of Mono Mac 6 cells with the residue of a crude dichloromethane extract of rose hip powder significantly and concentration dependently inhibited the lipopolysaccharide induced interleukin-6 release. Through bioassay-guided fractionation the immunomodulatory effect of the dichloromethane extract was correlated to a mixture of three triterpene acids; oleanolic acid, betulinic acid and ursolic acid (IC50 21 ± 6 µM). Further studies revealed that only oleanolic acid and ursolic acid, but not betulinic acid, could inhibit the lipopolysaccharide induced interleukin-6 release from Mono Mac 6 cells when tested separately. Combination of either oleanolic acid or ursolic acid with betulinic acid enhanced the immunomodulatory effect of the two triterpene acids.
