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En-qin Xia - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted extraction of oleanolic Acid and Ursolic Acid from ligustrum lucidum ait
    Ultrasonics Sonochemistry, 2012
    Co-Authors: En-qin Xia, Guifang Deng, Yajun Guo
    Abstract:

    Abstract Oleanolic Acid and Ursolic Acid are the main bioactive compounds in fruit of Ligustrum lucidum Ait, which possess anti-inflammatory, antioxidative, antiprotozoal, antimutagenic and anticancer properties. In this study, the ultrasound-assisted extraction of oleanolic Acid and Ursolic Acid from L. lucidum Ait was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, extraction temperature and extraction time, on extraction efficiencies of oleanolic Acid and Ursolic Acid from L. lucidum were evaluated. The influence of experimental parameters on extraction efficiency of Ursolic Acid was more significant than that of oleanolic Acid. The optimal extraction conditions were 95% ethanol, the ratio of material to liquid at 1:20, and extraction for 10 min at 40 °C under ultrasonic irradiation. Under the optimal conditions, the yields of oleanolic Acid and Ursolic Acid were 6.3 ± 0.25 and 9.8 ± 0.30 mg/g, respectively. The results indicated that the ultrasound-assisted extraction is a very useful method for the extraction of oleanolic Acid and Ursolic Acid from L. lucidum.

  • Microwave-Assisted Extraction of Oleanolic Acid and Ursolic Acid from Ligustrum lucidum Ait
    International journal of molecular sciences, 2011
    Co-Authors: En-qin Xia, Bo-wei Wang, Li-li Zhu, Yang Song
    Abstract:

    Oleanolic Acid and Ursolic Acid are the main active components in fruit of Ligustrum lucidum Ait, and possess anticancer, antimutagenic, anti-inflammatory, antioxidative and antiprotozoal activities. In this study, microwave-assisted extraction of oleanolic Acid and Ursolic Acid from Ligustrum lucidum was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, microwave power, extraction temperature and microwave time, on the extraction efficiencies of oleanolic Acid and Ursolic Acid from Ligustrum lucidum were evaluated. The influence of experimental parameters on the extraction efficiency of Ursolic Acid was more significant than that of oleanolic Acid (p < 0.05). The optimal extraction conditions were 80% ethanol aqueous solution, the ratio of material to liquid was 1:15, and extraction for 30 min at 70 °C under microwave irradiation of 500 W. Under optimal conditions, the yields of oleanolic Acid and Ursolic Acid were 4.4 ± 0.20 mg/g and 5.8 ± 0.15 mg/g, respectively. The results obtained are helpful for the full utilization of Ligustrum lucidum, which also indicated that microwave-assisted extraction is a very useful method for extraction of oleanolic Acid and Ursolic Acid from plant materials.

Li Kai-quan - One of the best experts on this subject based on the ideXlab platform.

  • Studies of Extraction Effect of Ursolic Acid by Cellulose
    Journal of Anhui Agricultural Sciences, 2007
    Co-Authors: Li Kai-quan
    Abstract:

    In lab experiment with the leaf of Eriobotrya Japonica Lindl.as tested material,the exacting treatment with the fibre enzyme pre-fermentation and the directly exacting treatment without enzyme were compared to confirm the optimum condition for increasing extraction rate of Ursolic Acid by cellulose.In former treatment,the cellulose preparation at 0.5% of dry weight of martial was added into 1 kg crud power leaf of Eriobotrya Japonica after the crud power was wet by warm water,then fermented for 48 h at room temperature,later the Ursolic Acid was extracted by ethanol.In later treatment,the Ursolic Acid was extracted directly by ethanol circumfluence.Results showed that the extracting rate of Ursolic Acid in adding cellulose enzyme group was obviously higher than that in no cellulose enzyme group(P0.01),suggesting that using cellulose enzyme could significantly enhance the extraction rate of Ursolic Acid.

  • Preparation and Quantitative Analysis of Ursolic Acid Separation from Herba Hedyotidis Corymbosae
    Journal of Yichun University, 2005
    Co-Authors: Li Kai-quan
    Abstract:

    Objective: To separate and extract the Ursolic Acid from Hedyotis corymbosa Lam by adopting the method of "ethanol extracting and agglutination" and to determine the content of Ursolic Acid . Method: Using spectrum to identify the structure of Ursolic Acid by IR, MS,1HNMR 13CNMR. Adetermination method of Ursolic Acid by reversed-phase high performance liquid chromtograph was established. The chromatographic column, SymmetryShield RP18 (3.9×150 mm,5 μm), methanol-water (88:12, V/V) mobile phase with 1.0 mL/min flow rate, the detected wavelength (210 nm), and the column temperature(25 ℃) were adopted. Result: The compound is recognized to be Ursolic Acid. The calibration curve of Ursolic Acid by HPLC was linear under the content of 4.5～22.5 μg, the correlation coefficient was over 0.9998. The content is 99.82% and the gain rate is 3.65‰. Conclusion: The preparation method is practical and feasible. The purity of Ursolic Acid is high and the safety is good. The analytical method is simple, accurate and has good repeatability and wider linear range. It can be adapted to evaluate the quality of Ursolic Acid.

  • Extracting and Identifying of Ursolic Acid from Hedyotis Corymbosa Lam.
    Journal of Jiangxi Normal University, 2005
    Co-Authors: Li Kai-quan
    Abstract:

    Adopting the method of ethanol extracting and agglutination separating to extract Ursolic Acid from Hedyotis corymbosa Lam. and using spectrum to identify it's structure. The compound is recognized to be Ursolic Acid, the content is 99.58% by HPLC, and the yield is 3.12‰. The method is practical and feasible. The purity of Ursolic Acid is high and the safety is good.

  • Extracting and Identifying Ursolic Acid from Hedyotis Corymbosa Lam.
    Journal of Yichun University, 2004
    Co-Authors: Li Kai-quan
    Abstract:

    Objective: To extract, separate and identify Ursolic Acid in Hedyotis corymbosa Lam.Methods: Adopting the method of ethanol extracting and agglutination separating to extract Ursolic Acid and using spectrum to identify its’structure. Results: The compound is recognized to be Ursolic Acid, the content is 99.58% by HPLC, and the gain rate is 3.12‰. Conclusion: The method is practical and feasible. The purity of Ursolic Acid is high and the safety is good.

  • Preparation and HPLC Analysis of Ursolic Acid From Eriobotrya japonica Lindl
    2004
    Co-Authors: Li Kai-quan
    Abstract:

    Adopting the method of ethanol extraction and agglomerate separation to prepare Ursolic Acid from Eriobotrya japonica Lindl. A method for determination of Ursolic Acid in sample by HPLC was established. The content of Ursolic Acid was determined by HPLC with methanol-H_2O (88∶12) as mobile phase at a flow rate of 1 mL/min, and detection wavelength at 210 nm. A Symmetry Shield RP18 column was used at 25 ℃. A good linearity could be obtained in the range of 4.4～22.0 μg (r=0.999 2) for Ursolic Acid .The average recovery of Ursolic Acid was 99.32% with RSD of 0.70%. The content determination is accurate and reliable, and can be used to determine the content of Ursolic Acid.

Miguel Ángel Medina - One of the best experts on this subject based on the ideXlab platform.

  • Effects of Ursolic Acid on different steps of the angiogenic process.
    Biochemical and biophysical research communications, 2004
    Co-Authors: Casimiro Cárdenas, Ana R. Quesada, Miguel Ángel Medina
    Abstract:

    Ursolic Acid is a triterpenoid with pleiotropic biological effects. In this report, we study the effects of Ursolic Acid on different key steps of angiogenesis. Our results show that Ursolic Acid is able to inhibit key steps of angiogenesis in vitro, including endothelial cell proliferation, migration, and differentiation. At the same time, it seems to stimulate other key steps of angiogenesis, such as extracellular matrix degradation by MMP-2 and urokinase. Although Ursolic Acid can inhibit in vivo angiogenesis in the CAM assay, the different signs of the effects it causes on different steps of angiogenesis force one to be cautious concerning its anti-angiogenic potential.

Yajun Guo - One of the best experts on this subject based on the ideXlab platform.

  • ultrasound assisted extraction of oleanolic Acid and Ursolic Acid from ligustrum lucidum ait
    Ultrasonics Sonochemistry, 2012
    Co-Authors: En-qin Xia, Guifang Deng, Yajun Guo
    Abstract:

    Abstract Oleanolic Acid and Ursolic Acid are the main bioactive compounds in fruit of Ligustrum lucidum Ait, which possess anti-inflammatory, antioxidative, antiprotozoal, antimutagenic and anticancer properties. In this study, the ultrasound-assisted extraction of oleanolic Acid and Ursolic Acid from L. lucidum Ait was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, extraction temperature and extraction time, on extraction efficiencies of oleanolic Acid and Ursolic Acid from L. lucidum were evaluated. The influence of experimental parameters on extraction efficiency of Ursolic Acid was more significant than that of oleanolic Acid. The optimal extraction conditions were 95% ethanol, the ratio of material to liquid at 1:20, and extraction for 10 min at 40 °C under ultrasonic irradiation. Under the optimal conditions, the yields of oleanolic Acid and Ursolic Acid were 6.3 ± 0.25 and 9.8 ± 0.30 mg/g, respectively. The results indicated that the ultrasound-assisted extraction is a very useful method for the extraction of oleanolic Acid and Ursolic Acid from L. lucidum.

Yang Song - One of the best experts on this subject based on the ideXlab platform.

  • Microwave-Assisted Extraction of Oleanolic Acid and Ursolic Acid from Ligustrum lucidum Ait
    International journal of molecular sciences, 2011
    Co-Authors: En-qin Xia, Bo-wei Wang, Li-li Zhu, Yang Song
    Abstract:

    Oleanolic Acid and Ursolic Acid are the main active components in fruit of Ligustrum lucidum Ait, and possess anticancer, antimutagenic, anti-inflammatory, antioxidative and antiprotozoal activities. In this study, microwave-assisted extraction of oleanolic Acid and Ursolic Acid from Ligustrum lucidum was investigated with HPLC-photodiode array detection. Effects of several experimental parameters, such as type and concentration of extraction solvent, ratio of liquid to material, microwave power, extraction temperature and microwave time, on the extraction efficiencies of oleanolic Acid and Ursolic Acid from Ligustrum lucidum were evaluated. The influence of experimental parameters on the extraction efficiency of Ursolic Acid was more significant than that of oleanolic Acid (p < 0.05). The optimal extraction conditions were 80% ethanol aqueous solution, the ratio of material to liquid was 1:15, and extraction for 30 min at 70 °C under microwave irradiation of 500 W. Under optimal conditions, the yields of oleanolic Acid and Ursolic Acid were 4.4 ± 0.20 mg/g and 5.8 ± 0.15 mg/g, respectively. The results obtained are helpful for the full utilization of Ligustrum lucidum, which also indicated that microwave-assisted extraction is a very useful method for extraction of oleanolic Acid and Ursolic Acid from plant materials.