The Experts below are selected from a list of 6891 Experts worldwide ranked by ideXlab platform
Mehdi Mirza - One of the best experts on this subject based on the ideXlab platform.
-
recovery of water soluble constituents of Rose Oil using simultaneous distillation extraction
Flavour and Fragrance Journal, 2005Co-Authors: Mohammad H. Eikani, Fereshteh Golmohammad, Soosan Rowshanzamir, Mehdi MirzaAbstract:Recovery of water-soluble constituents of Rose Oil from aqueous solutions using simultaneous distillation–extraction was studied. Experiments were carried out using a modified Likens–Nickerson apparatus. A preliminary study was first performed on aqueous 2-phenylethanol solutions. Using n-butyl acetate as the solvent, the effects of extraction time, solvent volume and solute concentration on recovery yields were investigated. The optimum experimental conditions obtained from the preliminary study were applied to extract Rose Oil constituents from actual aqueous solutions, such as Rose water, second distillation water and residual water of Iranian Rose (Rosa damascena Mill). The results show that a representative extract with recovery of the most valuable constituents of Rose Oil was carried out successfully. Copyright © 2005 John Wiley & Sons, Ltd.
-
Recovery of water-soluble constituents of Rose Oil using simultaneous distillation–extraction
Flavour and Fragrance Journal, 2005Co-Authors: Mohammad H. Eikani, Fereshteh Golmohammad, Soosan Rowshanzamir, Mehdi MirzaAbstract:Recovery of water-soluble constituents of Rose Oil from aqueous solutions using simultaneous distillation–extraction was studied. Experiments were carried out using a modified Likens–Nickerson apparatus. A preliminary study was first performed on aqueous 2-phenylethanol solutions. Using n-butyl acetate as the solvent, the effects of extraction time, solvent volume and solute concentration on recovery yields were investigated. The optimum experimental conditions obtained from the preliminary study were applied to extract Rose Oil constituents from actual aqueous solutions, such as Rose water, second distillation water and residual water of Iranian Rose (Rosa damascena Mill). The results show that a representative extract with recovery of the most valuable constituents of Rose Oil was carried out successfully. Copyright © 2005 John Wiley & Sons, Ltd.
Krasimir Rusanov - One of the best experts on this subject based on the ideXlab platform.
-
Exploring the capacity of endophytic fungi isolated from medicinal plants for fermentation and phenolics biotransformation of Rose Oil distillation wastewater
Biotechnology & Biotechnological Equipment, 2019Co-Authors: Mila Rusanova, Krasimir Rusanov, Veronika Butterweck, Ivan AtanassovAbstract:AbstractA total of 139 endophytic fungal isolates derived from 20 medicinal and aromatic plants were tested for their capacity to ferment Rose Oil distillation wastewater (RODW). Internal transcribed spacer sequence-based phylogenetic analysis affiliated the collected isolates to seven genera and nine different species, of which Alternaria alternata was the most widely represented. The fungal fermentation of RODW was evaluated through analysis of the sugar and phenolics content and phenolics composition. After cultivation in a small volume of RODW, the performed cluster analysis grouped the isolates in four distinct clusters according to their capacity to ferment RODW sugars and phenolics. Further larger volume shake-flask cultivation of a selected subset of fungi with a pronounced RODW fermentation capacity, outlined three groups of isolates with a distinct pattern and dynamics of sugars and phenolics fermentation. High-performance liquid chromatography analysis of RODW phenolics extracts following the f...
-
Exploring the capacity of endophytic fungi isolated from medicinal plants for fermentation and phenolics biotransformation of Rose Oil distillation wastewater
2019Co-Authors: Mila Rusanova, Krasimir Rusanov, Veronika Butterweck, Ivan AtanassovAbstract:A total of 139 endophytic fungal isolates derived from 20 medicinal and aromatic plants were tested for their capacity to ferment Rose Oil distillation wastewater (RODW). Internal transcribed spacer sequence-based phylogenetic analysis affiliated the collected isolates to seven genera and nine different species, of which Alternaria alternata was the most widely represented. The fungal fermentation of RODW was evaluated through analysis of the sugar and phenolics content and phenolics composition. After cultivation in a small volume of RODW, the performed cluster analysis grouped the isolates in four distinct clusters according to their capacity to ferment RODW sugars and phenolics. Further larger volume shake-flask cultivation of a selected subset of fungi with a pronounced RODW fermentation capacity, outlined three groups of isolates with a distinct pattern and dynamics of sugars and phenolics fermentation. High-performance liquid chromatography analysis of RODW phenolics extracts following the fermentation by the selected fungal isolates showed two main types of changes in phenolics composition: (1) changes in the relative abundance of part of the RODW phenolic compounds, mainly due to utilization of sugar residues from the RODW phenolic glycosides and (2) biosynthesis of new phenolic compounds, most of which are specific to the endophytic fungal isolates used for RODW fermentation. The overall results of the study clearly demonstrate that endophytic fungal isolates represent a rich and untapped source for efficient fermentation of RODW and other agro-industrial phenolics-rich wastewaters and highlight their capacity for directional changes and modification of RODW phenolics composition towards further wastewater phenolics valorization.
-
A Polyphenol-Enriched Fraction of Rose Oil Distillation Wastewater Inhibits Cell Proliferation, Migration and TNF-α-Induced VEGF Secretion in Human Immortalized Keratinocytes.
Planta medica, 2016Co-Authors: Jonas Wedler, Krasimir Rusanov, Ivan Atanassov, Veronika ButterweckAbstract:Water steam distillation of Rose flowers separates the essential Oil from the polyphenol-containing Rose Oil distillation wastewater. Recently, a strategy was developed to separate Rose Oil distillation wastewater into a polyphenol depleted water fraction and a polyphenol-enriched fraction [RF20-(SP-207)]. The objective of the present study was to investigate RF20-(SP-207) and fraction F(IV), augmented in quercetin and ellagic acid, for possible antiproliferative effects in immortalized human keratinocytes (HaCaT) since Rose petals are known to contain compounds with potential antiproliferative activity. RF20-(SP-207) revealed dose-dependent antiproliferative activity (IC50 of 9.78 µg/mL). In a nontoxic concentration of 10 µg/mL, this effect was stronger than that of the two positive controls LY294002 (10 µM, PI3 K-inhibitor, 30 % inhibition) and NVP-BEZ235 (100 nM, dual PI3 K/mTOR inhibitor, 30 % inhibition) and clearly exceeded the antiproliferative action of quercetin (50 µM, 25 % inhibition) and ellagic acid (1 µM, 15 % inhibition). Time-lapse microscopy detected a significant impairment of cell migration of RF20-(SP-207) and F(IV). At concentrations of 10 µg/mL of both, extract and fraction, cell migration was strongly suppressed (51 % and 28 % gap closure, respectively, compared to 95 % gap closure 24 hours after control treatment). The suppression of cell migration was comparable to the positive controls LY294002, NVP-BEZ235, and quercetin. Furthermore, basal and TNF-α-stimulated VEGF-secretion was significantly reduced by RF20-(SP-207) and F(IV) at 10 µg/mL (44 % vs. untreated control). In conclusion, RF20-(SP-207) showed promising antiproliferative and antimigratory effects and could be developed as a supportive, therapy against hyperproliferation-involved skin diseases.
-
Recovery of polyphenols from Rose Oil distillation wastewater using adsorption resins--a pilot study.
Planta medica, 2014Co-Authors: Krasimir Rusanov, Mila Rusanova, Ivan Atanassov, Eliane Garo, Orlando Fertig, Matthias Hamburger, Veronika ButterweckAbstract:The production of Rose Oil from Rose flowers by water steam distillation leaves a water fraction of the distillate as main part of the waste. Therefore, the Rose Oil distillation wastewater represents a serious environmental problem due to the high content of polyphenols which are difficult to decompose and have to be considered as biopollutants when discarded into the drainage system and rivers. On the other hand, natural polyphenols are valuable compounds with useful properties as bioactive substances. Until now there is no established practice for processing of Rose Oil distillation wastewater and utilization of contained substances. Thus, it was the aim of this study to develop a strategy to separate this wastewater into a polyphenol depleted water fraction and a polyphenol enriched fraction which could be developed into innovative value-added products. In a first step, the phytochemical profile of Rose Oil distillation wastewater was determined. Its HPLC-PDA-MS analysis revealed the presence of flavan-3-ols, flavanones, flavonols and flavones. In a second step, the development of a stepwise concentration of Rose Oil distillation wastewater was performed. The concentration process includes a filtration process to eliminate suspended solids in the wastewater, followed by adsorption of the contained phenolic compounds onto adsorption resins (XAD and SP). Finally, desorption of the polyphenol fraction from the resin matrix was achieved using ethanol and/or aqueous ethanol. The result of the process was a wastewater low in soluble organic compounds and an enriched polyphenol fraction (RF20 SP-207). The profile of this fraction was similar to that of Rose Oil distillation wastewater and showed the presence of flavonols such as quercetin and kaempferol glycosides as major metabolites. These compounds were isolated from the enriched polyphenol fraction and their structures confirmed by NMR. In summary, a pilot medium scale system was developed using adsorption resins for the recovery of polyphenols from Rose Oil distillation wastewater suggesting an industrial scalability of the process.
-
Flower phenotype variation, essential Oil variation and genetic diversity among Rosa alba L. accessions used for Rose Oil production in Bulgaria
Scientia Horticulturae, 2013Co-Authors: Krasimir Rusanov, Mila Rusanova, Natasha Kovacheva, Ivan AtanassovAbstract:a b s t r a c t Flower biometric analysis of 38 Rosa alba L. accessions from the genetic resources collection of the Institute of Roses, Essential and Medical Cultures (IREMC), Bulgaria, revealed the existence of two distinct flower phenogroups (PG: 1 and PG: 2), which differ significantly in flower weight, number of flower petals and number of anthers. GC/MS analysis of Rose Oil samples distilled from the analyzed accessions identified a total of 25 compounds of which 18 compounds were detected in all samples. PCA analysis based on GC/MS data of Rose Oil samples did not show clear clustering of the Rose Oils obtained from the two flower phenogroups. Although t-test analysis showed statistically significant variation of the abundances of 6 compounds at P < 0.05 and 4 compounds at P < 0.01 between the two identified phenogroups, the ranges of variation of these compounds among the PG: 1 plants largely overlapped those of the PG: 2 plants. Microsatellite analysis at 9 SSR loci of the studied 38 accessions as well as R. alba Maxima and R. alba Semiplena accessions revealed their clonal origin from a common ancestor. The selection of a small subset of PG: 1 accessions representing the overall diversity of the composition of Rose Oils distilled from the studied accessions is proposed for industrial production of quality planting material. The necessity to enlarge the R. alba genetic resources pool used for industrial cultivation and Rose Oil production is further discussed.
Mohammad H. Eikani - One of the best experts on this subject based on the ideXlab platform.
-
recovery of water soluble constituents of Rose Oil using simultaneous distillation extraction
Flavour and Fragrance Journal, 2005Co-Authors: Mohammad H. Eikani, Fereshteh Golmohammad, Soosan Rowshanzamir, Mehdi MirzaAbstract:Recovery of water-soluble constituents of Rose Oil from aqueous solutions using simultaneous distillation–extraction was studied. Experiments were carried out using a modified Likens–Nickerson apparatus. A preliminary study was first performed on aqueous 2-phenylethanol solutions. Using n-butyl acetate as the solvent, the effects of extraction time, solvent volume and solute concentration on recovery yields were investigated. The optimum experimental conditions obtained from the preliminary study were applied to extract Rose Oil constituents from actual aqueous solutions, such as Rose water, second distillation water and residual water of Iranian Rose (Rosa damascena Mill). The results show that a representative extract with recovery of the most valuable constituents of Rose Oil was carried out successfully. Copyright © 2005 John Wiley & Sons, Ltd.
-
Recovery of water-soluble constituents of Rose Oil using simultaneous distillation–extraction
Flavour and Fragrance Journal, 2005Co-Authors: Mohammad H. Eikani, Fereshteh Golmohammad, Soosan Rowshanzamir, Mehdi MirzaAbstract:Recovery of water-soluble constituents of Rose Oil from aqueous solutions using simultaneous distillation–extraction was studied. Experiments were carried out using a modified Likens–Nickerson apparatus. A preliminary study was first performed on aqueous 2-phenylethanol solutions. Using n-butyl acetate as the solvent, the effects of extraction time, solvent volume and solute concentration on recovery yields were investigated. The optimum experimental conditions obtained from the preliminary study were applied to extract Rose Oil constituents from actual aqueous solutions, such as Rose water, second distillation water and residual water of Iranian Rose (Rosa damascena Mill). The results show that a representative extract with recovery of the most valuable constituents of Rose Oil was carried out successfully. Copyright © 2005 John Wiley & Sons, Ltd.
Albena Stoyanova - One of the best experts on this subject based on the ideXlab platform.
-
Valorization of wastes from the Rose Oil industry
Reviews in Environmental Science and Bio Technology, 2017Co-Authors: Anton Slavov, Ivelina Vasileva, Lyubomir Stefanov, Albena StoyanovaAbstract:Every year, the Rose Oil industry generates large amounts of wastes due to the low content of essential Oil in the fresh Rose petals. Most of the distilleries simply discard the waste biomass which could lead to ecological problems in the nearby locations. Various methods for Rose waste utilization have been successfully developed during the past years for the purpose of solving this problem. The aim of the present review is to summarize the possible approaches to the valorization of Rose waste biomass, and to outline future trends. Apart from the common methods of disposal, i.e. composting, use for animal forage or simply discarding the wastes, there are several other promising and widely investigated strategies for utilization: aroma substance extraction, recovery of valuable biologically active substances and their application to food industry or medicine, and studies focused on increasing the essential Oil yield. Novel approaches include the extraction of polysaccharides from the biomass and introduction of integrated methods for more complete valorization of the Rose waste biomass. Nevertheless, most of the methods for Rose waste valorization still remain on laboratory scale. This review shows that the potential of the Rose Oil industry wastes is still underexplored and further studies in this direction are needed. Graphical Abstract
-
Chemical Composition and Antimicrobial Activity of Historical Rose Oil from Bulgaria
Journal of Essential Oil Bearing Plants, 2009Co-Authors: Velizar Gochev, Leopold Jirovetz, Katrin Wlcek, Gerhard Buchbauer, Erich Schmidt, Albena Stoyanova, Anna DobrevaAbstract:Abstract The chemical composition of historical Rose Oil (produced in 1944) from Bulgaria was investigated by GC and GC-MS. The Rose Oil was found to be rich in citronellol (23.4 %), geraniol (19.0 %), nonadecane (11.9 %), and nerol (7.5 %). In general, the chemical composition of this historical Rose Oil corresponded exactly to the criteria stated in the chromatographic profile of ISO 9842:2003. Furthermore, the antimicrobial activity of this Rose Oil sample was tested against three Gram-positive bacteria and three Gram-negative bacteria as well as against two yeasts. This old Oil still demonstrated antimicrobial effects against all of the used microorganisms with Bacillus cereus found to be the most susceptible and Pseudomonas aeruginosa the most resistible strain among the tested microorganisms. In addition, a comparison of these analytical and antimicrobial results with data of some confirmed varieties from the type “Bulgarian red oleaginous Rose” is given.
-
solid phase microextraction gas chromatographic and olfactory analysis of the scent and fixative properties of the essential Oil of rosa damascena l from china
Flavour and Fragrance Journal, 2005Co-Authors: Leopold Jirovetz, Gerhard Buchbauer, Albena Stoyanova, Anastasia Balinova, Zhang Guangjiun, Ma XihanAbstract:The composition of the essential Oil and the SPME-headspace volatiles of Rosa damascena L. (Rosaceae) from China were investigated by gas chromatographic–spectroscopic (GC–FID and GC–MS) and olfactoric methods to identify those compounds responsible for the characteristic, pleasant floral odour of these valuable, high-priced flower Oils. Monoterpenes especially were found to be fragrance target compounds, while additionally some aliphatic hydrocarbons will have fixative effects and therefore are responsible for a longer-lasting odour impression (tested by olfactory evaluation after application to human skin) of this Rose Oil. The main compounds [concentration higher than 3.0%; calculated as %-peak area of GC–FID analyses using a polar (carbowax) column] of the 71 totally identified constituents of the two Rose samples are as follows: Chinese Rose Oil: citronellol (30.71%), nonadecane (16.95%), geraniol (16.11%), nerol (7.57%), heneicosane (7.04%) and 9-eicosene (4.71%); and SPME-headspace of the Chinese Rose Oil: citronellol (37.05%), geraniol (18.62%), nerol (9.91%) and methyl eugenol (3.56%). A correlation of the identified constituents of the two Rose Oil samples with their single odour impressions is also given and the significant change of the composition of the SPME-headspace samples after Rose Oil application on human skin discussed. Copyright © 2004 John Wiley & Sons, Ltd.
-
Solid phase microextraction/gas chromatographic and olfactory analysis of the scent and fixative properties of the essential Oil of Rosa damascena L. from China
Flavour and Fragrance Journal, 2004Co-Authors: Leopold Jirovetz, Gerhard Buchbauer, Albena Stoyanova, Anastasia Balinova, Zhang Guangjiun, Ma XihanAbstract:The composition of the essential Oil and the SPME-headspace volatiles of Rosa damascena L. (Rosaceae) from China were investigated by gas chromatographic–spectroscopic (GC–FID and GC–MS) and olfactoric methods to identify those compounds responsible for the characteristic, pleasant floral odour of these valuable, high-priced flower Oils. Monoterpenes especially were found to be fragrance target compounds, while additionally some aliphatic hydrocarbons will have fixative effects and therefore are responsible for a longer-lasting odour impression (tested by olfactory evaluation after application to human skin) of this Rose Oil. The main compounds [concentration higher than 3.0%; calculated as %-peak area of GC–FID analyses using a polar (carbowax) column] of the 71 totally identified constituents of the two Rose samples are as follows: Chinese Rose Oil: citronellol (30.71%), nonadecane (16.95%), geraniol (16.11%), nerol (7.57%), heneicosane (7.04%) and 9-eicosene (4.71%); and SPME-headspace of the Chinese Rose Oil: citronellol (37.05%), geraniol (18.62%), nerol (9.91%) and methyl eugenol (3.56%). A correlation of the identified constituents of the two Rose Oil samples with their single odour impressions is also given and the significant change of the composition of the SPME-headspace samples after Rose Oil application on human skin discussed. Copyright © 2004 John Wiley & Sons, Ltd.
Ma Xihan - One of the best experts on this subject based on the ideXlab platform.
-
solid phase microextraction gas chromatographic and olfactory analysis of the scent and fixative properties of the essential Oil of rosa damascena l from china
Flavour and Fragrance Journal, 2005Co-Authors: Leopold Jirovetz, Gerhard Buchbauer, Albena Stoyanova, Anastasia Balinova, Zhang Guangjiun, Ma XihanAbstract:The composition of the essential Oil and the SPME-headspace volatiles of Rosa damascena L. (Rosaceae) from China were investigated by gas chromatographic–spectroscopic (GC–FID and GC–MS) and olfactoric methods to identify those compounds responsible for the characteristic, pleasant floral odour of these valuable, high-priced flower Oils. Monoterpenes especially were found to be fragrance target compounds, while additionally some aliphatic hydrocarbons will have fixative effects and therefore are responsible for a longer-lasting odour impression (tested by olfactory evaluation after application to human skin) of this Rose Oil. The main compounds [concentration higher than 3.0%; calculated as %-peak area of GC–FID analyses using a polar (carbowax) column] of the 71 totally identified constituents of the two Rose samples are as follows: Chinese Rose Oil: citronellol (30.71%), nonadecane (16.95%), geraniol (16.11%), nerol (7.57%), heneicosane (7.04%) and 9-eicosene (4.71%); and SPME-headspace of the Chinese Rose Oil: citronellol (37.05%), geraniol (18.62%), nerol (9.91%) and methyl eugenol (3.56%). A correlation of the identified constituents of the two Rose Oil samples with their single odour impressions is also given and the significant change of the composition of the SPME-headspace samples after Rose Oil application on human skin discussed. Copyright © 2004 John Wiley & Sons, Ltd.
-
Solid phase microextraction/gas chromatographic and olfactory analysis of the scent and fixative properties of the essential Oil of Rosa damascena L. from China
Flavour and Fragrance Journal, 2004Co-Authors: Leopold Jirovetz, Gerhard Buchbauer, Albena Stoyanova, Anastasia Balinova, Zhang Guangjiun, Ma XihanAbstract:The composition of the essential Oil and the SPME-headspace volatiles of Rosa damascena L. (Rosaceae) from China were investigated by gas chromatographic–spectroscopic (GC–FID and GC–MS) and olfactoric methods to identify those compounds responsible for the characteristic, pleasant floral odour of these valuable, high-priced flower Oils. Monoterpenes especially were found to be fragrance target compounds, while additionally some aliphatic hydrocarbons will have fixative effects and therefore are responsible for a longer-lasting odour impression (tested by olfactory evaluation after application to human skin) of this Rose Oil. The main compounds [concentration higher than 3.0%; calculated as %-peak area of GC–FID analyses using a polar (carbowax) column] of the 71 totally identified constituents of the two Rose samples are as follows: Chinese Rose Oil: citronellol (30.71%), nonadecane (16.95%), geraniol (16.11%), nerol (7.57%), heneicosane (7.04%) and 9-eicosene (4.71%); and SPME-headspace of the Chinese Rose Oil: citronellol (37.05%), geraniol (18.62%), nerol (9.91%) and methyl eugenol (3.56%). A correlation of the identified constituents of the two Rose Oil samples with their single odour impressions is also given and the significant change of the composition of the SPME-headspace samples after Rose Oil application on human skin discussed. Copyright © 2004 John Wiley & Sons, Ltd.
