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Junghoon Yoon - One of the best experts on this subject based on the ideXlab platform.
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Roseovarius ponticola sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from seawater.
Archives of microbiology, 2018Co-Authors: Sooyeon Park, Jimin Park, Jung-sook Lee, Junghoon YoonAbstract:A lipolytic, Gram-negative, aerobic, non-motile and pleomorphic bacterial strain, designated W-SW3T, was isolated from seawater around Wando, an island of South Korea, and its taxonomic position was determined using a polyphasic approach. Its optimal growth occurred at 30 °C, pH 7.0-8.0 and 2.0-4.0% (w/v) NaCl. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain W-SW3T belonged to the genus Roseovarius of the family Rhodobacteraceae, by showing the highest 16S rRNA gene sequence similarity value (97.3%) to the type strain of Roseovarius nanhaiticus. Strain W-SW3T exhibited 16S rRNA gene sequence similarities of 93.2-95.6% to the type strains of the other Roseovarius species. Ubiquinone-10 was found as the predominant ubiquinone and C18:1ω7c and C16:0 were found as the major fatty acids. Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid were major polar lipids detected. The DNA G + C content of strain W-SW3T was 63.9 mol%. Mean DNA-DNA relatedness values of strain W-SW3T with the type strains of R. nanhaiticus and R. antarcticus were 18 and 9%, respectively. The phenotypic properties, together with the phylogenetic and genetic data, made it possible strain W-SW3T to be separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain W-SW3T represents a novel species of the genus Roseovarius, for which the name Roseovarius ponticola sp. nov. is proposed. The type strain is W-SW3T (= KCTC 62424T = KACC 19615T = NBRC 113186T).
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Roseovarius aestuariivivens sp. nov., isolated from a tidal flat.
International journal of systematic and evolutionary microbiology, 2017Co-Authors: Sooyeon Park, Sun Young Yoon, Yong-taek Jung, Junghoon YoonAbstract:A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterium, designated GHTF-24T, was isolated from a tidal flat on the South Sea, South Korea, and subjected to polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GHTF-24T fell within the clade comprising the type strains of species of the genus Roseovarius . Strain GHTF-24T exhibited 16S rRNA gene sequence similarity values of 97.0 % to each of Roseovarius halotolerans HJ50T and Roseovarius pacificus 81-2T and of 91.6–96.5 % to the type strains of the other species of the genus Roseovarius . Strain GHTF-24T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids of strain GHTF-24T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GHTF-24T was 62.3 mol%, and its mean DNA–DNA relatedness values with the type strains of R. halotolerans and R. pacificus were 13–16 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHTF-24T is separated from other recognized species of the genus Roseovarius . On the basis of the data presented, strain GHTF-24T is considered to represent a novel species of the genus Roseovarius , for which the name Roseovarius aestuariivivens sp. nov. is proposed. The type strain is GHTF-24T (=KCTC 52454T=NBRC 112420T).
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Correspondence
2016Co-Authors: Junghoon Yoon, Republic Of KoreaAbstract:A Gram-negative, motile, ovoid- to rod-shaped bacterial strain, designated MA1-10T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. Strain MA1-10T grew optimally at pH 7.0–8.0, at 30 6C and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain MA1-10T clustered with Roseovarius crassostreae CV919-312T, with which it exhibited 97.1 % sequence similarity, at a bootstrap resampling value of 96.2%. It exhibited 93.3–95.8 % 16S rRNA gene sequence similarity to the type strains of other recognized Roseovarius species. Strain MA1-10T contained Q-10 as the predominant ubiquinone and C18: 1v7c as the major fatty acid, which is consistent with data for the genus Roseovarius. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified amino-lipid and an unidentified lipid. The DNA G+C content of strain MA1-10T was 55.4 mol%. Mean DNA–DNA relatedness between strain MA1-10T and R. crassostreae DSM 16950T was 13%. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain MA1-10T could be distinguished from all recognized Roseovarius species. On the basis of the data presented, strain MA1-10T is considered to represent a nove
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Roseovarius scapharcae sp nov isolated from ark shell scapharca broughtonii
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Sooyeon Park, Jimin Park, Junghoon YoonAbstract:A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated MA4-5T, was isolated from ark shell (Scapharca broughtonii) collected from the South Sea, South Korea. The novel strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences showed that strain MA4-5T forms a coherent cluster with the type strains of Roseovarius albus, Roseovarius aestuarii and Roseovarius nubinhibens, sharing 97.0–99.2 % sequence similarity. It exhibited 16S rRNA gene sequence similarity of 93.1–96.1 % to the type strains of other Roseovarius species. Strain MA4-5T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain MA4-5T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain MA4-5T was 53.8 mol% and its mean DNA–DNA relatedness values with the type strains of R. albus, R. aestuarii and R. nubinhibens were 11–26 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, indicated that strain MA4-5T is separate from recognized species of the genus Roseovarius. On the basis of the data presented, strain MA4-5T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius scapharcae sp. nov. is proposed. The type strain is MA4-5T ( = KCTC 42703T = NBRC 111226T).
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Roseovarius aquimarinus sp. nov., a slightly halophilic bacterium isolated from seawater.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Hyeonji Kang, Junghoon Yoon, Jong-hwa Kim, Che Ok Jeon, Wonyong KimAbstract:A Gram-stain-negative, non-spore-forming, rod-shaped, motile, facultatively anaerobic bacterium, designated CAU 1059T, was isolated from a seawater sample from Jeju Island, Republic of Korea. The bacterium grew optimally at 37 °C, at pH 7.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1059T belonged to the genus Roseovarius. It exhibited only 91.5–96.9 % sequence similarity to the type strains of recognized Roseovarius species. Similar to other species of the genus Roseovarius, strain CAU 1059T had ubiquinone-10 (Q-10) as the predominant ubiquinone and C16 : 0 and summed feature 8 (C18 : 1ω7c/ω6c) as the major fatty acids. The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine; three unidentified phospholipids, two aminolipids, an aminophospholipid and nine other lipids were also found. The G+C content of the genomic DNA was 61.9 mol%. On the basis of the data provided, strain CAU 1059T should be classified as representing a novel species of the genus Roseovarius, for which the name Roseovarius aquimarinus sp. nov. is proposed. The type strain is CAU 1059T ( = KCTC 32014T = CCUG 64792T).
Sooyeon Park - One of the best experts on this subject based on the ideXlab platform.
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Roseovarius ponticola sp. nov., a novel lipolytic bacterium of the family Rhodobacteraceae isolated from seawater.
Archives of microbiology, 2018Co-Authors: Sooyeon Park, Jimin Park, Jung-sook Lee, Junghoon YoonAbstract:A lipolytic, Gram-negative, aerobic, non-motile and pleomorphic bacterial strain, designated W-SW3T, was isolated from seawater around Wando, an island of South Korea, and its taxonomic position was determined using a polyphasic approach. Its optimal growth occurred at 30 °C, pH 7.0-8.0 and 2.0-4.0% (w/v) NaCl. Neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain W-SW3T belonged to the genus Roseovarius of the family Rhodobacteraceae, by showing the highest 16S rRNA gene sequence similarity value (97.3%) to the type strain of Roseovarius nanhaiticus. Strain W-SW3T exhibited 16S rRNA gene sequence similarities of 93.2-95.6% to the type strains of the other Roseovarius species. Ubiquinone-10 was found as the predominant ubiquinone and C18:1ω7c and C16:0 were found as the major fatty acids. Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid and one unidentified aminolipid were major polar lipids detected. The DNA G + C content of strain W-SW3T was 63.9 mol%. Mean DNA-DNA relatedness values of strain W-SW3T with the type strains of R. nanhaiticus and R. antarcticus were 18 and 9%, respectively. The phenotypic properties, together with the phylogenetic and genetic data, made it possible strain W-SW3T to be separated from other recognized species of the genus Roseovarius. On the basis of the data presented, strain W-SW3T represents a novel species of the genus Roseovarius, for which the name Roseovarius ponticola sp. nov. is proposed. The type strain is W-SW3T (= KCTC 62424T = KACC 19615T = NBRC 113186T).
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Roseovarius aestuariivivens sp. nov., isolated from a tidal flat.
International journal of systematic and evolutionary microbiology, 2017Co-Authors: Sooyeon Park, Sun Young Yoon, Yong-taek Jung, Junghoon YoonAbstract:A Gram-stain-negative, non-motile, aerobic and coccoid, ovoid or rod-shaped bacterium, designated GHTF-24T, was isolated from a tidal flat on the South Sea, South Korea, and subjected to polyphasic taxonomic study. The novel strain grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain GHTF-24T fell within the clade comprising the type strains of species of the genus Roseovarius . Strain GHTF-24T exhibited 16S rRNA gene sequence similarity values of 97.0 % to each of Roseovarius halotolerans HJ50T and Roseovarius pacificus 81-2T and of 91.6–96.5 % to the type strains of the other species of the genus Roseovarius . Strain GHTF-24T contained Q-10 as the predominant ubiquinone and C18 : 1 ω7c as the major fatty acid. The major polar lipids of strain GHTF-24T were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GHTF-24T was 62.3 mol%, and its mean DNA–DNA relatedness values with the type strains of R. halotolerans and R. pacificus were 13–16 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain GHTF-24T is separated from other recognized species of the genus Roseovarius . On the basis of the data presented, strain GHTF-24T is considered to represent a novel species of the genus Roseovarius , for which the name Roseovarius aestuariivivens sp. nov. is proposed. The type strain is GHTF-24T (=KCTC 52454T=NBRC 112420T).
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Roseovarius scapharcae sp nov isolated from ark shell scapharca broughtonii
International Journal of Systematic and Evolutionary Microbiology, 2015Co-Authors: Sooyeon Park, Jimin Park, Junghoon YoonAbstract:A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated MA4-5T, was isolated from ark shell (Scapharca broughtonii) collected from the South Sea, South Korea. The novel strain grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences showed that strain MA4-5T forms a coherent cluster with the type strains of Roseovarius albus, Roseovarius aestuarii and Roseovarius nubinhibens, sharing 97.0–99.2 % sequence similarity. It exhibited 16S rRNA gene sequence similarity of 93.1–96.1 % to the type strains of other Roseovarius species. Strain MA4-5T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain MA4-5T were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain MA4-5T was 53.8 mol% and its mean DNA–DNA relatedness values with the type strains of R. albus, R. aestuarii and R. nubinhibens were 11–26 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, indicated that strain MA4-5T is separate from recognized species of the genus Roseovarius. On the basis of the data presented, strain MA4-5T is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius scapharcae sp. nov. is proposed. The type strain is MA4-5T ( = KCTC 42703T = NBRC 111226T).
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Pontivivens insulae gen. nov., sp. nov., isolated from seawater.
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Sooyeon Park, Jimin Park, Yong-taek Jung, Sung-min Won, Junghoon YoonAbstract:A Gram-stain-negative, aerobic, non-motile and coccoid, ovoid or rod-shaped bacterial strain, designated GYSW-23T, was isolated from seawater off Geoje island in the South Sea, South Korea. Strain GYSW-23T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of approximately 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences revealed that strain GYSW-23T forms a distinct evolutionary lineage independent of other taxa of the family Rhodobacteraceae. It exhibited 16S rRNA gene sequence similarity values of 94.0, 93.5, 93.4 and 93.4 % to the type strains of Roseovarius aestuarii, Ruegeria marina, Roseovarius pacificus and Oceanicola litoreus, respectively, and 93.6 % to ‘Actibacterium atlanticum’ 22II-S11-z10. Strain GYSW-23T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids of strain GYSW-23T were phosphatidylcholine, phosphatidylglycerol and one unidentified aminolipid. The fatty acid and polar lipid profiles of strain GYSW-23T were distinguishable from those of the phylogenetically related taxa. The DNA G+C content of strain GYSW-23T was 60.6 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain GYSW-23T is considered to represent a novel species of a new genus in the family Rhodobacteraceae, for which the name Pontivivens insulae gen. nov., sp. nov. is proposed. The type strain of Pontivivens insulae is GYSW-23T ( = KCTC 42458T = CECT 8812T).
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AliiRoseovarius pelagivivens gen. nov., sp. nov., isolated from seawater, and reclassification of three species of the genus Roseovarius as AliiRoseovarius crassostreae comb. nov., AliiRoseovarius halocynthiae comb. nov. and AliiRoseovarius sediminil
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Sooyeon Park, Jimin Park, Chul-hyung Kang, Junghoon YoonAbstract:A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated GYSW-22T, was isolated from seawater off Geoje Island in the South Sea, South Korea. Strain GYSW-22T grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences showed that strain GYSW-22T and the type strains of Roseovarius crassostreae, Roseovarius halocynthiae and Roseovarius sediminilitoris form a coherent cluster, independent of phylogenetic lineages or clusters comprising the type strains of other species of the genus Roseovarius. Strain GYSW-22T exhibited 16S rRNA gene sequence similarities of 97.2, 96.6 and 96.3 % to R. halocynthiae MA1-10T, R. crassostreae CV919-312T and R. sediminilitoris M-M10T, respectively, and of 92.6–94.7 % to the type strains of other species of the genus Roseovarius. Strain GYSW-22T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain GYSW-22T was 59.0 mol% and its mean DNA–DNA relatedness value with R. halocynthiae MA1-10T was 15 %. On the basis of the data presented, we propose strain GYSW-22T represents a novel species of a new genus, AliiRoseovarius pelagivivens gen. nov., sp. nov. The type strain of the type species is GYSW-22T ( = KCTC 42459T = CECT 8811T). In this study, it is also proposed that Roseovarius crassostreae, Roseovarius halocynthiae and Roseovarius sediminilitoris be reclassified into the new genus as AliiRoseovarius crassostreae comb. nov. (type strain CV919-312T = ATCC BAA-1102T = DSM 16950T), AliiRoseovarius halocynthiae comb. nov. (type strain MA1-10T = KCTC 23462T = CCUG 60745T) and AliiRoseovarius sediminilitoris comb. nov. (type strain M-M10T = KCTC 23959T = CCUG 62413T), respectively.
Ronald P. Kiene - One of the best experts on this subject based on the ideXlab platform.
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Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate- demethylating bacteria from marine environments
International Journal of Systematic and Evolutionary Microbiology, 2003Co-Authors: José M. González, William B. Whitman, Joseph S. Covert, James R. Henriksen, Frank Mayer, Birgit E. Scharf, Rüdiger Schmitt, Alison Buchan, Jed A. Fuhrman, Ronald P. KieneAbstract:Three Gram-negative, rod-shaped, aerobic bacteria that were capable of degrading dimethylsulfoniopropionate (DMSP) were isolated from marine waters. These isolates (DSS-3T, DSS-10 and ISMT) exhibited the ability to demethylate and cleave DMSP, as well as to degrade other sulfur compounds related to DMSP that are cycled in marine environments. Intracellular poly-β-hydroxybutyrate inclusions, surface blebs and one polar, complex flagellum that rotated exclusively in the clockwise direction were observed for DSS-3T. The outer membrane of ISMT was separated from the cytoplasm at the poles in a toga-like morphology. The primary fatty acid in both strains was C18 : 1 ω7c. DNA G+C contents for the isolates were 68·0±0·1, 68·1±0·1 and 66·0±0·2 mol% for DSS-3T, DSS-10 and ISMT, respectively. 16S rRNA gene sequence analyses placed these organisms within the Roseobacter lineage of the α-Proteobacteria. Closely related species were Silicibacter lacuscaerulensis and Ruegeria atlantica (DSS-3T and DSS-10) and Roseovarius tolerans (ISMT). Neither DSS-3T nor ISMT exhibited 16S rRNA similarity >97 % or DNA–DNA hybridization values >45 % to their nearest described relatives. Genotypic and phenotypic analyses support the creation of two novel species: Silicibacter pomeroyi sp. nov. with strain DSS-3T (=ATCC 700808T=DSM 15171T) as the type strain, and Roseovarius nubinhibens sp. nov. with strain ISMT (=ATCC BAA-591T=DSM 15170T) as the type strain.
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Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments.
International journal of systematic and evolutionary microbiology, 2003Co-Authors: José M. González, William B. Whitman, Joseph S. Covert, James R. Henriksen, Frank Mayer, Rüdiger Schmitt, Alison Buchan, Jed A. Fuhrman, Birgit Scharf, Ronald P. KieneAbstract:Three Gram-negative, rod-shaped, aerobic bacteria that were capable of degrading dimethylsulfoniopropionate (DMSP) were isolated from marine waters. These isolates (DSS-3(T), DSS-10 and ISM(T)) exhibited the ability to demethylate and cleave DMSP, as well as to degrade other sulfur compounds related to DMSP that are cycled in marine environments. Intracellular poly-beta-hydroxybutyrate inclusions, surface blebs and one polar, complex flagellum that rotated exclusively in the clockwise direction were observed for DSS-3(T). The outer membrane of ISM(T) was separated from the cytoplasm at the poles in a toga-like morphology. The primary fatty acid in both strains was C(18 : 1)omega7c. DNA G+C contents for the isolates were 68.0+/-0.1, 68.1+/-0.1 and 66.0+/-0.2 mol% for DSS-3(T), DSS-10 and ISM(T), respectively. 16S rRNA gene sequence analyses placed these organisms within the Roseobacter lineage of the alpha-PROTEOBACTERIA: Closely related species were Silicibacter lacuscaerulensis and Ruegeria atlantica (DSS-3(T) and DSS-10) and Roseovarius tolerans (ISM(T)). Neither DSS-3(T) nor ISM(T) exhibited 16S rRNA similarity >97 % or DNA-DNA hybridization values >45 % to their nearest described relatives. Genotypic and phenotypic analyses support the creation of two novel species: Silicibacter pomeroyi sp. nov. with strain DSS-3(T) (=ATCC 700808(T)=DSM 15171(T)) as the type strain, and Roseovarius nubinhibens sp. nov. with strain ISM(T) (=ATCC BAA-591(T)=DSM 15170(T)) as the type strain.
Sojung Kang - One of the best experts on this subject based on the ideXlab platform.
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ruegeria halocynthiae sp nov isolated from the sea squirt halocynthia roretzi
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Sooyeon Park, Sojung Kang, Taekwang Oh, Junghoon YoonAbstract:A Gram-negative, motile, ovoid- to rod-shaped bacterial strain, designated MA1-10T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. Strain MA1-10T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain MA1-10T clustered with Roseovarius crassostreae CV919-312T, with which it exhibited 97.1 % sequence similarity, at a bootstrap resampling value of 96.2 %. It exhibited 93.3–95.8 % 16S rRNA gene sequence similarity to the type strains of other recognized Roseovarius species. Strain MA1-10T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid, which is consistent with data for the genus Roseovarius . The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid. The DNA G+C content of strain MA1-10T was 55.4 mol%. Mean DNA–DNA relatedness between strain MA1-10T and R. crassostreae DSM 16950T was 13 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain MA1-10T could be distinguished from all recognized Roseovarius species. On the basis of the data presented, strain MA1-10T is considered to represent a novel species of the genus Roseovarius , for which the name Roseovarius halocynthiae sp. nov. is proposed; the type strain is MA1-10T ( = KCTC 23462T = CCUG 60745T).
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Roseovarius halocynthiae sp. nov., isolated from the sea squirt Halocynthia roretzi
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: Young-ok Kim, Sooyeon Park, Sojung Kang, Hee Jeong Kong, Woo-jin Kim, Kyung-kil Kim, Junghoon YoonAbstract:A Gram-negative, motile, ovoid- to rod-shaped bacterial strain, designated MA1-10T, was isolated from a sea squirt (Halocynthia roretzi) collected from the South Sea, Korea. Strain MA1-10T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain MA1-10T clustered with Roseovarius crassostreae CV919-312T, with which it exhibited 97.1 % sequence similarity, at a bootstrap resampling value of 96.2 %. It exhibited 93.3–95.8 % 16S rRNA gene sequence similarity to the type strains of other recognized Roseovarius species. Strain MA1-10T contained Q-10 as the predominant ubiquinone and C18 : 1ω7c as the major fatty acid, which is consistent with data for the genus Roseovarius . The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and an unidentified lipid. The DNA G+C content of strain MA1-10T was 55.4 mol%. Mean DNA–DNA relatedness between strain MA1-10T and R. crassostreae DSM 16950T was 13 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain MA1-10T could be distinguished from all recognized Roseovarius species. On the basis of the data presented, strain MA1-10T is considered to represent a novel species of the genus Roseovarius , for which the name Roseovarius halocynthiae sp. nov. is proposed; the type strain is MA1-10T ( = KCTC 23462T = CCUG 60745T).
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Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008Co-Authors: Junghoon Yoon, Sojung KangAbstract:A Gram-negative, motile, ovoid to rod-shaped bacterial strain, designated strain SMK-122T, was isolated from a Yellow Sea tidal flat located on the coast of Korea. Strain SMK-122T grew optimally at pH 7.0–8.0 and 30 °C. It contained Q-10 as the predominant ubiquinone and possessed C18 : 1 ω7c and C16 : 0 as the major fatty acids. The DNA G+C content was 58.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain SMK-122T fell within the genus Roseovarius, being closest to Roseovarius nubinhibens ISMT; the sequence similarities with respect to Roseovarius species ranged from 94.9 to 97.3 %. The mean value for DNA–DNA relatedness between strain SMK-122T and Rva. nubinhibens DSM 15170T was 13 %. Differential phenotypic properties of SMK-122T, together with its phylogenetic and genetic distinctiveness, revealed that this strain is distinct from recognized Roseovarius species. On this basis, strain SMK-122T represents a novel species of the genus Roseovarius, for which the name Roseovarius aestuarii sp. nov. is proposed. The type strain is SMK-122T (=KCTC 22174T =CCUG 55325T).
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Roseovarius aestuarii sp. nov., isolated from a tidal flat of the Yellow Sea in Korea.
International journal of systematic and evolutionary microbiology, 2008Co-Authors: Junghoon Yoon, Sojung KangAbstract:A Gram-negative, motile, ovoid to rod-shaped bacterial strain, designated strain SMK-122T, was isolated from a Yellow Sea tidal flat located on the coast of Korea. Strain SMK-122T grew optimally at pH 7.0-8.0 and 30 degrees C. It contained Q-10 as the predominant ubiquinone and possessed C18 : 1omega7c and C16 : 0 as the major fatty acids. The DNA G+C content was 58.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain SMK-122T fell within the genus Roseovarius, being closest to Roseovarius nubinhibens ISM(T); the sequence similarities with respect to Roseovarius species ranged from 94.9 to 97.3 %. The mean value for DNA-DNA relatedness between strain SMK-122T and Rva. nubinhibens DSM 15170T was 13 %. Differential phenotypic properties of SMK-122T, together with its phylogenetic and genetic distinctiveness, revealed that this strain is distinct from recognized Roseovarius species. On this basis, strain SMK-122T represents a novel species of the genus Roseovarius, for which the name Roseovarius aestuarii sp. nov. is proposed. The type strain is SMK-122T (=KCTC 22174T =CCUG 55325T).
Zongze Shao - One of the best experts on this subject based on the ideXlab platform.
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Roseovarius amoyensis sp. nov. and Muricauda amoyensis sp. nov., isolated from the Xiamen coast
International journal of systematic and evolutionary microbiology, 2019Co-Authors: Qiliang Lai, Peisheng Yan, Zongze ShaoAbstract:Strain GCL-8T and GCL-11T were isolated from Guanyinshan, a sandy beach on the Xiamen coast. Cells of the two strains were Gram-stain-negative, catalase-positive, oxidase-positive, rod-shaped and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains GCL-8T and GCL-11T belonged to the genera Roseovarius and Muricauda , with the highest sequence similarities to Roseovarius indicus B108T (96.5%) and Muricauda antarctica CGMCC 1.12174T (97.3%), respectively. The major fatty acids of strain GCL-8T were summed feature 8 (C18:1ω7c/ω6c), C19:0cyclo ω8c, C16:0 and C17:0, while the major fatty acids of strain GCL-11T were iso-C15:1 G, iso-C15:0 and iso-C17:0 3-OH. The G+C contents of the chromosomal DNA of strains GCL-8Tand GCL-11T were 64.1 mol% and 45.67 mol%, respectively. The respiratory quinones of strains GCL-8T and GCL-11T were Q-10 and MK-6, respectively. The polar lipids of strain GCL-8T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified aminolipid, three unidentified phospholipids and three unidentified polar lipids. The polar lipids of strain GCL-11Twere phosphatidylethanolamine, seven unidentified phospholipids and four unidentified polar lipids. The combined genotypic and phenotypic data show that strains GCL-8T and GCL-11T represent two novel species within genera Roseovarius and Muricauda , for which the names Roseovariu samoyensis sp. nov. and Muricauda amoyensis sp. nov. are proposed, with type strains GCL-8T (=MCCC 1A11651T = KCTC 52430T) and GCL-11T (=MCCC 1A11649T=KCTC 52432T).
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Roseovarius atlanticus sp. nov., isolated from surface seawater
International journal of systematic and evolutionary microbiology, 2015Co-Authors: Qiliang Lai, Zongze Shao, Chunming Dong, Xiupian Liu, Fengqin Sun, Peisheng YanAbstract:A taxonomic study was carried out on strain R12BT, which was isolated from surface seawater of the Atlantic Ocean. The bacterium was observed to be rod-shaped, Gram-stain-negative, oxidase-positive and weakly positive for catalase. Growth was observed at salinities of 0.5–15 % and at temperatures of 4–45 °C. The isolate was incapable of nitrate reduction and hydrolysis of gelatin, Tween 80 and aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R12BT belonged to the genus Roseovarius, with highest sequence similarity to Roseovarius indicus B108T (97.2 % 16S rRNA gene sequence similarity), followed by Roseovarius halotolerans HJ50T (96.8 %); other species of genus Roseovarius shared 93.0–96.2 % sequence similarities. The DNA–DNA hybridization estimate value between strain R12BT and R. indicus B108T was 23.2 ± 2.4 %. The average nucleotide identity between strain R12BT and R. indicus B108T was 77.1 %. The principal fatty acids were summed feature 8 (C18 : 1ω7c/ω6c) and C16 : 0. The G+C content of the chromosomal DNA was 63.6 mol%. The respiratory quinone was determined to be Q-10. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two aminolipids, two phospholipids and some unidentified lipids were present. The combined genotypic and phenotypic data show that strain R12BT represents a novel species of the genus Roseovarius, for which the name Roseovarius atlanticus sp. nov. is proposed, with the type strain R12BT ( = MCCC 1A09786T = KCTC 42506T).
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Roseovarius indicus sp nov isolated from deep sea water of the indian ocean
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Qiliang Lai, Fengqin Sun, Huanzi Zhong, Jianning Wang, Jun Yuan, Liping Wang, Tianling Zheng, Zongze ShaoAbstract:A taxonomic study was carried out on a novel bacterial strain, designated B108T, which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium, enriched from deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped and non-motile. Growth of strain B108T was observed in 1–15 % (w/v) NaCl and at 10–39 °C and it was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain B108T was most closely related to Roseovarius halotolerans HJ50T (97.1 % sequence similarity), followed by Roseovarius pacificus 81-2T (96.6 %) and Roseovarius aestuarii SMK-122T (95.2 %); other species shared <95.0 % sequence similarity. DNA–DNA hybridization tests showed that strain B108T had a low DNA–DNA relatedness to R. halotolerans HJ50T and R. pacificus 81-2T (48±4 % and 44±5 %, respectively). The predominant fatty acids were C16 : 0, C16 : 0 2-OH, summed feature 8 (C18 : 1ω7c/ω6c) and C19 : 0ω8c cyclo, which accounted for 84.2 % of the total cellular fatty acids. The G+C content of the chromosomal DNA was 63.6 mol%. The major respiratory quinone was ubiquinone 10 (Q10). Phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and some unidentified compounds were detected. These characteristics were in good agreement with those of members of the genus Roseovarius. The pufLM gene was also detected. According to its morphology, physiology, fatty acid composition and phylogenetic position based on 16S rRNA sequence data, the novel strain most appropriately belongs to the genus Roseovarius but can be readily distinguished from known species of this genus. Therefore, strain B108T represents a novel species, of the genus Roseovarius, for which the name Roseovarius indicus sp. nov. is proposed. The type strain is B108T ( = 2PR52-14T = CCTCC AB 208233T = LMG 24622T = MCCC 1A01227T).
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Roseovarius indicus sp. nov., isolated from deep-sea water of the Indian Ocean.
International journal of systematic and evolutionary microbiology, 2010Co-Authors: Qiliang Lai, Fengqin Sun, Huanzi Zhong, Jianning Wang, Jun Yuan, Liping Wang, Tianling Zheng, Zongze ShaoAbstract:A taxonomic study was carried out on a novel bacterial strain, designated B108(T), which was isolated from a polycyclic aromatic hydrocarbon (PAH)-degrading consortium, enriched from deep-sea water of the Indian Ocean. The isolate was Gram-reaction-negative, rod-shaped and non-motile. Growth of strain B108(T) was observed in 1-15 % (w/v) NaCl and at 10-39 °C and it was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain B108(T) was most closely related to Roseovarius halotolerans HJ50(T) (97.1 % sequence similarity), followed by Roseovarius pacificus 81-2(T) (96.6 %) and Roseovarius aestuarii SMK-122(T) (95.2 %); other species shared
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Roseovarius nanhaiticus sp. nov., a member of the Roseobacter clade isolated from marine sediment.
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Baojiang Wang, Qiliang Lai, Xiupian Liu, Fengqin Sun, Jie Luo, Zongze ShaoAbstract:An aerobic, Gram-staining-negative, rod or ovoid-shaped bacterial isolate, strain NH52JT, was isolated from a sandy sediment sample from the South China Sea. Strain NH52JT exhibited tumbling motility, formed beige or faint pink colonies, gave a positive reaction in tests for catalase and oxidase and required NaCl for growth. Optimal growth was observed at pH 7.8–9.3, at 30 °C and in the presence of 2.0–4.0 % (w/v) NaCl. The novel strain did not synthesize bacteriochlorophyll a, and the DNA G+C content was 62 %. The major fatty acids were C18 : 1 ω7c, C16 : 0 and C18 : 1 ω7c 11-methyl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NH52JT was affiliated to the genus Roseovarius of the class Alphaproteobacteria. Roseovarius pacificus and Roseovarius aestuarii were the most closely related recognized species to strain NH52JT with 16S rRNA gene sequence similarity values of 95.0 and 95.7 %, respectively. Sequence similarity values between strain NH52JT and other phylogenetically related species were all below 95.0 %. Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain NH52JT is considered to represent a novel species of the genus Roseovarius, for which the name Roseovarius nanhaiticus sp. nov. is proposed. The type strain is NH52JT (=LMG 24840T=CCTCC AB 208317T=MCCC 1A03543T).
