The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Sato Junko - One of the best experts on this subject based on the ideXlab platform.
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comparison of the morphological changes in the patellar tendon between meniscal bearing and rotating platform design prosthesis
Journal of Bone and Joint Surgery-british Volume, 2016Co-Authors: Mitsuhiro Takeda, Ishii Yoshinori, Noguchi Hideo, Sato JunkoAbstract:Introduction The low-contact stress (LCS) knee prosthesis is a mobile-bearing design with modifications to the tibial component that allow for meniscal-bearing (MB) or rotating-platform (RP). The MB design had nonconstrained anteroposterior and Rotational Movement, and the RP design has only nonconstrained Rotational Movement. The anterior soft tissues, including patellar tendon (PT), prevent anterior dislocation of the MB. The PT may consistently be exposed to overstressing. Therefore, we hypothesized that the PT thickness and width in MB prosthesis revealed more morphological changes than those of RP prosthesis due to degeneration of the PT induced by much mechanical stress of the MB Movement. To confirm this hypothesis, we analyze the PT thickness and width induced by mobile-bearing inserts. Objectives Sixty LCS prostheses in 30 patients were analyzed. The average follow-up time was 61 months. MB prosthesis was used on one side of the knee and RP prosthesis was used on the contralateral side of the knee. All patients were chosen from group with no clinical complication, and all had achieved passive full extension and at least 90°of flexion. The average Hospital for Special Surgery Score was 94.6 ± 2.7. Methods We measured the thickness and width of PT at joint line level, which were confirmed by sagittal section using ultrasound in knee extension between MB and RP design prosthesis. Results The mean thickness of PT was 4.7 mm (1.2) with MB and 4.7 mm (1.0) with RP design prosthesis. The mean width of PT was 30.6 mm (3.2) with MB and 31.3 mm (3.5) with RP design prosthesis. No significant differences were found between both groups. Conclusion The current results showed that the PT thickness and width in MB prosthesis did not reveal more morphological changes than those of RP prosthesis due to degeneration of the PT induced by much mechanical stress of the MB Movement. The possible reasons are the following: (1) We did not remove infra-patellar fat pad, which might play shock absorber of mechanical stress from MB, and prevent from significant degeneration of PT, (2) MB inserts did not stimulate the middle of the PT directly, unlike LCS A/P-Glide inserts, and might come into contact with the both ends of the PT and (3) MB inserts did not move so as to cause degeneration in the PT.
Keith Moffat - One of the best experts on this subject based on the ideXlab platform.
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design and signaling mechanism of light regulated histidine kinases
Journal of Molecular Biology, 2009Co-Authors: Andreas Moglich, Rebecca A Ayers, Keith MoffatAbstract:Abstract Signal transduction proteins are organized into sensor (input) domains that perceive a signal and, in response, regulate the biological activity of effector (output) domains. We reprogrammed the input signal specificity of a normally oxygen-sensitive, light-inert histidine kinase by replacing its chemosensor domain by a light-oxygen-voltage photosensor domain. Illumination of the resultant fusion kinase YF1 reduced net kinase activity by ∼ 1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo. Signals are transmitted from the light-oxygen-voltage sensor domain to the histidine kinase domain via a 40°–60° Rotational Movement within an α-helical coiled-coil linker; light is acting as a rotary switch. These signaling principles are broadly applicable to domains linked by α-helices and to chemo- and photosensors. Conserved sequence motifs guide the rational design of light-regulated variants of histidine kinases and other proteins.
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design and signaling mechanism of light regulated histidine kinases
Journal of Molecular Biology, 2009Co-Authors: Andreas Moglich, Rebecca A Ayers, Keith MoffatAbstract:Signal transduction proteins are organized into sensor (input) domains that perceive a signal and, in response, regulate the biological activity of effector (output) domains. We reprogrammed the input signal specificity of a normally oxygen-sensitive, light-inert histidine kinase by replacing its chemosensor domain by a light-oxygen-voltage photosensor domain. Illumination of the resultant fusion kinase YF1 reduced net kinase activity by approximately 1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo. Signals are transmitted from the light-oxygen-voltage sensor domain to the histidine kinase domain via a 40 degrees -60 degrees Rotational Movement within an alpha-helical coiled-coil linker; light is acting as a rotary switch. These signaling principles are broadly applicable to domains linked by alpha-helices and to chemo- and photosensors. Conserved sequence motifs guide the rational design of light-regulated variants of histidine kinases and other proteins.
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design and signaling mechanism of light regulated histidine kinases
Biophysical Journal, 2009Co-Authors: Andreas Moglich, Rebecca A Ayers, Keith MoffatAbstract:Signal transduction proteins are organized into sensor (input) domains that perceive a signal and, in response, regulate the biological activity of effector (output) domains. We reprogrammed the input signal specificity of a normally oxygen-sensitive, light-inert histidine kinase by replacing its chemosensor domain by a light-oxygen-voltage (LOV) photosensor domain. Illumination of the resultant fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo. Signals are transmitted from the LOV sensor domain to the histidine kinase domain via a 40-60° Rotational Movement within an α-helical coiled coil linker; light is acting as a rotary switch. These signaling principles are broadly applicable to domains linked by α-helices, and to both chemo- and photosensors. Conserved sequence motifs guide the rational design of light-regulated variants of histidine kinases and other proteins.
Yangyang Yang - One of the best experts on this subject based on the ideXlab platform.
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a photoregulated dna based rotary system and direct observation of its Rotational Movement
Chemistry: A European Journal, 2017Co-Authors: Yangyang Yang, Ryu Tashiro, Yuki Suzuki, Tomoko Emura, Kumi Hidaka, Hiroshi Sugiyama, Masayuki EndoAbstract:Various DNA-based nanodevices have been developed on the nanometer scale using light as regulation input. However, the programmed controllability is still a major challenge for these artificial nanodevices. Herein, we demonstrate a rotary DNA nanostructure in which the rotations are controlled by light. A bar-shaped DNA rotor, fabricated as a stiff double-crossover molecule, was placed on the top of a rectangular DNA tile. The photoresponsive oligonucleotides modified with azobenzenes were employed as switching motifs to release/trap the rotor at specific angular position on DNA tile by switching photoirradiations between ultraviolet and visible light. As a result, two reconfigurable states (perpendicular and parallel) of rotor were obtained, in which the angular changes were characterized by AFM and fluorescence quenching assays. Moreover, the reversible rotary motions during the photoirradiation were directly visualized on the DNA tile surface in a nanometer-scale precision using a second-scale scanning of the high-speed AFM.
Mitsuhiro Takeda - One of the best experts on this subject based on the ideXlab platform.
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comparison of the morphological changes in the patellar tendon between meniscal bearing and rotating platform design prosthesis
Journal of Bone and Joint Surgery-british Volume, 2016Co-Authors: Mitsuhiro Takeda, Ishii Yoshinori, Noguchi Hideo, Sato JunkoAbstract:Introduction The low-contact stress (LCS) knee prosthesis is a mobile-bearing design with modifications to the tibial component that allow for meniscal-bearing (MB) or rotating-platform (RP). The MB design had nonconstrained anteroposterior and Rotational Movement, and the RP design has only nonconstrained Rotational Movement. The anterior soft tissues, including patellar tendon (PT), prevent anterior dislocation of the MB. The PT may consistently be exposed to overstressing. Therefore, we hypothesized that the PT thickness and width in MB prosthesis revealed more morphological changes than those of RP prosthesis due to degeneration of the PT induced by much mechanical stress of the MB Movement. To confirm this hypothesis, we analyze the PT thickness and width induced by mobile-bearing inserts. Objectives Sixty LCS prostheses in 30 patients were analyzed. The average follow-up time was 61 months. MB prosthesis was used on one side of the knee and RP prosthesis was used on the contralateral side of the knee. All patients were chosen from group with no clinical complication, and all had achieved passive full extension and at least 90°of flexion. The average Hospital for Special Surgery Score was 94.6 ± 2.7. Methods We measured the thickness and width of PT at joint line level, which were confirmed by sagittal section using ultrasound in knee extension between MB and RP design prosthesis. Results The mean thickness of PT was 4.7 mm (1.2) with MB and 4.7 mm (1.0) with RP design prosthesis. The mean width of PT was 30.6 mm (3.2) with MB and 31.3 mm (3.5) with RP design prosthesis. No significant differences were found between both groups. Conclusion The current results showed that the PT thickness and width in MB prosthesis did not reveal more morphological changes than those of RP prosthesis due to degeneration of the PT induced by much mechanical stress of the MB Movement. The possible reasons are the following: (1) We did not remove infra-patellar fat pad, which might play shock absorber of mechanical stress from MB, and prevent from significant degeneration of PT, (2) MB inserts did not stimulate the middle of the PT directly, unlike LCS A/P-Glide inserts, and might come into contact with the both ends of the PT and (3) MB inserts did not move so as to cause degeneration in the PT.
Andreas Moglich - One of the best experts on this subject based on the ideXlab platform.
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design and signaling mechanism of light regulated histidine kinases
Journal of Molecular Biology, 2009Co-Authors: Andreas Moglich, Rebecca A Ayers, Keith MoffatAbstract:Abstract Signal transduction proteins are organized into sensor (input) domains that perceive a signal and, in response, regulate the biological activity of effector (output) domains. We reprogrammed the input signal specificity of a normally oxygen-sensitive, light-inert histidine kinase by replacing its chemosensor domain by a light-oxygen-voltage photosensor domain. Illumination of the resultant fusion kinase YF1 reduced net kinase activity by ∼ 1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo. Signals are transmitted from the light-oxygen-voltage sensor domain to the histidine kinase domain via a 40°–60° Rotational Movement within an α-helical coiled-coil linker; light is acting as a rotary switch. These signaling principles are broadly applicable to domains linked by α-helices and to chemo- and photosensors. Conserved sequence motifs guide the rational design of light-regulated variants of histidine kinases and other proteins.
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design and signaling mechanism of light regulated histidine kinases
Journal of Molecular Biology, 2009Co-Authors: Andreas Moglich, Rebecca A Ayers, Keith MoffatAbstract:Signal transduction proteins are organized into sensor (input) domains that perceive a signal and, in response, regulate the biological activity of effector (output) domains. We reprogrammed the input signal specificity of a normally oxygen-sensitive, light-inert histidine kinase by replacing its chemosensor domain by a light-oxygen-voltage photosensor domain. Illumination of the resultant fusion kinase YF1 reduced net kinase activity by approximately 1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo. Signals are transmitted from the light-oxygen-voltage sensor domain to the histidine kinase domain via a 40 degrees -60 degrees Rotational Movement within an alpha-helical coiled-coil linker; light is acting as a rotary switch. These signaling principles are broadly applicable to domains linked by alpha-helices and to chemo- and photosensors. Conserved sequence motifs guide the rational design of light-regulated variants of histidine kinases and other proteins.
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design and signaling mechanism of light regulated histidine kinases
Biophysical Journal, 2009Co-Authors: Andreas Moglich, Rebecca A Ayers, Keith MoffatAbstract:Signal transduction proteins are organized into sensor (input) domains that perceive a signal and, in response, regulate the biological activity of effector (output) domains. We reprogrammed the input signal specificity of a normally oxygen-sensitive, light-inert histidine kinase by replacing its chemosensor domain by a light-oxygen-voltage (LOV) photosensor domain. Illumination of the resultant fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo. Signals are transmitted from the LOV sensor domain to the histidine kinase domain via a 40-60° Rotational Movement within an α-helical coiled coil linker; light is acting as a rotary switch. These signaling principles are broadly applicable to domains linked by α-helices, and to both chemo- and photosensors. Conserved sequence motifs guide the rational design of light-regulated variants of histidine kinases and other proteins.
