The Experts below are selected from a list of 603 Experts worldwide ranked by ideXlab platform
Jakub Tolar - One of the best experts on this subject based on the ideXlab platform.
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bacterial meningitis from Rothia mucilaginosa in patients with malignancy or undergoing hematopoietic stem cell transplantation
Pediatric Blood & Cancer, 2008Co-Authors: Paul Harkermurray, Mark R Schleiss, Patricia Ferrieri, Jakub TolarAbstract:Opportunistic infections contribute to morbidity and mortality of patients undergoing hematopoietic stem cell transplantation and treatment for malignancies. Rothia mucilaginosa, a gram-positive bacterium, is responsible for rare, but often fatal meningitis in severely immunocompromised patients. We describe two cases of meningitis from discrete strains of R. mucilaginosa on our pediatric bone marrow transplant unit, summarize the published cases of R. mucilaginosa meningitis in oncology and stem cell transplant patients, and provide updated recommendations regarding the use of antibiotic therapy in this patient population. Pediatr Blood Cancer 2008;50:673–676. © 2007 Wiley-Liss, Inc.
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Bacterial meningitis from Rothia mucilaginosa in patients with malignancy or undergoing hematopoietic stem cell transplantation.
Pediatric blood & cancer, 2008Co-Authors: Alisa B Lee, Patricia Ferrieri, Mark R Schleiss, Paul Harker-murray, Jakub TolarAbstract:Opportunistic infections contribute to morbidity and mortality of patients undergoing hematopoietic stem cell transplantation and treatment for malignancies. Rothia mucilaginosa, a gram-positive bacterium, is responsible for rare, but often fatal meningitis in severely immunocompromised patients. We describe two cases of meningitis from discrete strains of R. mucilaginosa on our pediatric bone marrow transplant unit, summarize the published cases of R. mucilaginosa meningitis in oncology and stem cell transplant patients, and provide updated recommendations regarding the use of antibiotic therapy in this patient population.
Masahiro Aida - One of the best experts on this subject based on the ideXlab platform.
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a selective medium for Rothia mucilaginosa and its distribution in oral cavities
Journal of Microbiological Methods, 2012Co-Authors: Taira Kobayashi, Satoshi Uchibori, Haruhiko Goto, Osamu Tsuzukibashi, Masahiro AidaAbstract:Abstract A selective medium for Rothia mucilaginosa (RMSM) was developed to examine the population of R. mucilaginosa in oral cavities. The growth recovery of R. mucilaginosa on RMSM was 85.1% relative to HI medium. R. mucilaginosa was detected at 3.4% of total bacteria from stimulated saliva of 8 subjects.
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a novel selective medium for the isolation and distribution of Rothia dentocariosa in oral cavities
Journal of Microbiological Methods, 2012Co-Authors: Satoshi Uchibori, Osamu Tsudukibashi, Haruhiko Goto, Taira Kobayashi, Masahiro AidaAbstract:Abstract Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart Infusion (HI). Average growth recovery of R. dentocariosa on RDSM was 93.8% that of HI medium. Growth of other bacteria including Rothia mucilaginosa , Neisseria , Actinomyces , or streptococci was remarkably inhibited on the selective medium. Clinical efficacy was evaluated by the recovery of R. dentocariosa on RDSM from the stimulated saliva samples of 8 volunteers. R. dentocariosa was detected at 2.6 (range: 1.0–4.6) % to total bacteria of 8.0 × 10 7 CFU/ml on BHI in their oral cavities. The new selective medium, RDSM, was highly selective for R. dentocariosa and was useful for the isolation of R. dentocariosa from clinical samples.
Alexander Indra - One of the best experts on this subject based on the ideXlab platform.
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Rothia mucilaginosa bacteremia a 10 year experience of a pediatric tertiary care cancer center
Pediatric Blood & Cancer, 2019Co-Authors: Fiona Poyer, Waltraud Friesenbichler, Herbert Pichler, Georg Mann, Michael Dworzak, Caroline Hutter, Christina Peters, Alexander IndraAbstract:: Rothia mucilaginosa is part of the oral and upper respiratory tract flora. Usually, this gram-positive coccus is not pathogenic; however, in the setting of immunosuppressed hosts, it can cause life-threatening infections as an opportunistic pathogen. Among a cohort of 1511 hematologic-oncologic patients at a pediatric tertiary care cancer center, we identified five cancer patients (0.35%) within a period of 10 years having a proven Rothia mucilaginosa bacteremia (1 culture positive: n = 3/5; > 1 culture positive: n = 2/5). With prompt and adequate antibiotic treatment, infection resolved rapidly before recovery of neutrophils and without any sequelae, suggesting that Rothia mucilaginosa bacteremia without organ involvement is not exceptionally problematic in pediatric cancer patients.
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Rothia mucilaginosa bacteremia: A 10‐year experience of a pediatric tertiary care cancer center
Pediatric Blood & Cancer, 2019Co-Authors: Fiona Poyer, Waltraud Friesenbichler, Herbert Pichler, Georg Mann, Alexander Indra, Michael Dworzak, Caroline Hutter, Christina Peters, Andishe AttarbaschiAbstract:Rothia mucilaginosa is part of the oral and upper respiratory tract flora. Usually, this gram-positive coccus is not pathogenic; however, in the setting of immunosuppressed hosts, it can cause life-threatening infections as an opportunistic pathogen. Among a cohort of 1511 hematologic-oncologic patients at a pediatric tertiary care cancer center, we identified five cancer patients (0.35%) within a period of 10 years having a proven Rothia mucilaginosa bacteremia (1 culture positive: n = 3/5; > 1 culture positive: n = 2/5). With prompt and adequate antibiotic treatment, infection resolved rapidly before recovery of neutrophils and without any sequelae, suggesting that Rothia mucilaginosa bacteremia without organ involvement is not exceptionally problematic in pediatric cancer patients.
Taira Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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isolation and identification methods of Rothia species in oral cavities
Journal of Microbiological Methods, 2017Co-Authors: Osamu Tsuzukibashi, Satoshi Uchibori, Tomomi Hashizumetakizawa, Takayuki Nambu, Chiho Mashimo, Masanori Saito, Koji Umezawa, Taira Kobayashi, Tomoko OchiaiAbstract:Rothia dentocariosa and Rothia mucilaginosa which are Gram-positive bacteria are part of the normal flora in the human oral cavity and pharynx. Furthermore, Rothia aeria, which was first isolated from air samples in the Russian space station Mir, is predicted to be an oral inhabitant. Immunocompromised patients are often infected by these organisms, leading to various systemic diseases. The involvement of these organisms in oral infections has attracted little attention, and their distribution in the oral cavity has not been fully clarified because of difficulties in accurately identifying these organisms. A suitable selective medium for oral Rothia species, including R. aeria, is necessary to assess the veritable prevalence of these organisms in the oral cavity. To examine the bacterial population in the oral cavity, a novel selective medium (ORSM) was developed for isolating oral Rothia species in this study. ORSM consists of tryptone, sodium gluconate, Lab-Lemco powder, sodium fluoride, neutral acriflavin, lincomycin, colistin, and agar. The average growth recovery of oral Rothia species on ORSM was 96.7% compared with that on BHI-Y agar. Growth of other representative oral bacteria, i.e. genera Streptococcus, Actinomyces, Neisseria, and Corynebacterium, was remarkably inhibited on the selective medium. PCR primers were designed based on partial sequences of the 16S rDNA genes of oral Rothia species. These primers reacted to each organism and did not react to other non-oral Rothia species or representative oral bacteria. These results indicated that these primers are useful for identifying oral Rothia species. A simple multiplex PCR procedure using these primers was a reliable method of identifying oral Rothia species. The proportion of oral Rothia species in saliva samples collected from 20 subjects was examined by culture method using ORSM. Rothia dentocariosa, Rothia mucilaginosa, and R. aeria accounted for 1.3%, 5.9%, and 0.8% of the total cultivable bacteria number on BHI-Y agar in the oral cavities of all subjects, respectively. It was indicated that among oral Rothia species, R. mucilaginosa is most predominant in the oral cavity of humans. A novel selective medium, ORSM, was useful for isolating each oral Rothia species.
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a selective medium for Rothia mucilaginosa and its distribution in oral cavities
Journal of Microbiological Methods, 2012Co-Authors: Taira Kobayashi, Satoshi Uchibori, Haruhiko Goto, Osamu Tsuzukibashi, Masahiro AidaAbstract:Abstract A selective medium for Rothia mucilaginosa (RMSM) was developed to examine the population of R. mucilaginosa in oral cavities. The growth recovery of R. mucilaginosa on RMSM was 85.1% relative to HI medium. R. mucilaginosa was detected at 3.4% of total bacteria from stimulated saliva of 8 subjects.
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a novel selective medium for the isolation and distribution of Rothia dentocariosa in oral cavities
Journal of Microbiological Methods, 2012Co-Authors: Satoshi Uchibori, Osamu Tsudukibashi, Haruhiko Goto, Taira Kobayashi, Masahiro AidaAbstract:Abstract Rothia dentocariosa is an indigenous microbial flora of dental plaque. To examine the bacterium population in oral cavities, a novel selective medium, designated RDSM, was developed for the isolation of R. dentocariosa. RDSM was prepared by adding 0.5 μg/ml of lincomycin, 10 μg/ml of colistin, and 40% sucrose to Heart Infusion (HI). Average growth recovery of R. dentocariosa on RDSM was 93.8% that of HI medium. Growth of other bacteria including Rothia mucilaginosa , Neisseria , Actinomyces , or streptococci was remarkably inhibited on the selective medium. Clinical efficacy was evaluated by the recovery of R. dentocariosa on RDSM from the stimulated saliva samples of 8 volunteers. R. dentocariosa was detected at 2.6 (range: 1.0–4.6) % to total bacteria of 8.0 × 10 7 CFU/ml on BHI in their oral cavities. The new selective medium, RDSM, was highly selective for R. dentocariosa and was useful for the isolation of R. dentocariosa from clinical samples.
Michael Dworzak - One of the best experts on this subject based on the ideXlab platform.
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Rothia mucilaginosa bacteremia a 10 year experience of a pediatric tertiary care cancer center
Pediatric Blood & Cancer, 2019Co-Authors: Fiona Poyer, Waltraud Friesenbichler, Herbert Pichler, Georg Mann, Michael Dworzak, Caroline Hutter, Christina Peters, Alexander IndraAbstract:: Rothia mucilaginosa is part of the oral and upper respiratory tract flora. Usually, this gram-positive coccus is not pathogenic; however, in the setting of immunosuppressed hosts, it can cause life-threatening infections as an opportunistic pathogen. Among a cohort of 1511 hematologic-oncologic patients at a pediatric tertiary care cancer center, we identified five cancer patients (0.35%) within a period of 10 years having a proven Rothia mucilaginosa bacteremia (1 culture positive: n = 3/5; > 1 culture positive: n = 2/5). With prompt and adequate antibiotic treatment, infection resolved rapidly before recovery of neutrophils and without any sequelae, suggesting that Rothia mucilaginosa bacteremia without organ involvement is not exceptionally problematic in pediatric cancer patients.
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Rothia mucilaginosa bacteremia: A 10‐year experience of a pediatric tertiary care cancer center
Pediatric Blood & Cancer, 2019Co-Authors: Fiona Poyer, Waltraud Friesenbichler, Herbert Pichler, Georg Mann, Alexander Indra, Michael Dworzak, Caroline Hutter, Christina Peters, Andishe AttarbaschiAbstract:Rothia mucilaginosa is part of the oral and upper respiratory tract flora. Usually, this gram-positive coccus is not pathogenic; however, in the setting of immunosuppressed hosts, it can cause life-threatening infections as an opportunistic pathogen. Among a cohort of 1511 hematologic-oncologic patients at a pediatric tertiary care cancer center, we identified five cancer patients (0.35%) within a period of 10 years having a proven Rothia mucilaginosa bacteremia (1 culture positive: n = 3/5; > 1 culture positive: n = 2/5). With prompt and adequate antibiotic treatment, infection resolved rapidly before recovery of neutrophils and without any sequelae, suggesting that Rothia mucilaginosa bacteremia without organ involvement is not exceptionally problematic in pediatric cancer patients.
