Saccharina Japonica

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Delin Duan - One of the best experts on this subject based on the ideXlab platform.

  • Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina Japonica
    Journal of Applied Phycology, 2020
    Co-Authors: Jie Zhang, Norishige Yotsukura, Jian-ting Yao, Chikako Nagasato, Alexander Jueterbock, Jorge Assis, Delin Duan
    Abstract:

    Natural hybridization can play a significant role in evolutionary processes and influence the adaptive diversification and speciation of brown seaweeds. However, this phenomenon is as yet unknown in Saccharina kelps. Saccharina angustata and two varieties of Saccharina Japonica (S. Japonica var. Japonica and S. Japonica var. diabolica) partly overlap in distribution along the Pacific coast of Hokkaido, which makes them a good model system to study hybridization and introgression among species of the genus Saccharina. Based on 13 highly variable nuclear microsatellites and a mitochondrial marker, we assessed the genetic diversity levels of S. angustata for the first time and populations from Muroran to Shiranuka (western part of the Pacific coast in Hokkaido) exhibited highest genetic diversity. Genetic diversity of S. Japonica was higher in S. Japonica var. Japonica as compared with S. Japonica var. diabolica. There was significant genetic differentiation (FST > 0.25, p 

  • Status of genetic studies and breeding of Saccharina Japonica in China
    Journal of Oceanology and Limnology, 2020
    Co-Authors: Xiuliang Wang, Jie Zhang, Jian-ting Yao, Delin Duan
    Abstract:

    Saccharina Japonica is one of the most important economic brown seaweeds. It is intensively cultivated on large scales in a number of Asian countries. The current annual, global production is about 8 million tons valued as about 4 million US dollars. Considerable efforts have been made to S. Japonica in China since the 1950s on its cultivation. To further advance the cultivation of this species, detailed research of genetics and breeding studies are required. Recently, with the advancement of sequencing techniques, the genomics and comparative transcriptomics data were yielded, and quantitative trait locus (QTL) mapping has been conducted, along with genetic linkage maps constructed to this species. New strains have been bred and selected, with better characteristics, e.g. higher seawater temperature resistances and higher yields. In this review, we present the current status of genetic and breeding studies that have been performed to S. Japonica in China, and provide guidelines for future developments in the areas of genetic selection and breeding for this species.

  • Transcriptome sequencing of Saccharina Japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis
    2019
    Co-Authors: Zhanru Shao, Xiuliang Wang, Zhihang Chen, Pengyan Zhang, Shun Liu, Delin Duan
    Abstract:

    Abstract Background: Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in brown seaweed Saccharina Japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown.Results: With WGCNA and trend analysis of 20,940 known genes and 4,264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and imm upregulated 3 gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions: The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. Japonica developments.Keywords: Alginate, Mannitol, Transcriptome, Regulatory networks, Growth, Development, Saccharina Japonica

  • verification of the Saccharina Japonica translocon tic20 and its localization in the chloroplast membrane in diatoms
    International Journal of Molecular Sciences, 2019
    Co-Authors: Zhihang Chen, Xiuliang Wang, Zhanru Shao, Shuang Li, Delin Duan
    Abstract:

    Tic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga Saccharina Japonica. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an “M”-type Nin-Cin-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga Phaeodactylum tricornutum, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in S. Japonica is needed.

  • Effect of domestication on the genetic diversity and structure of Saccharina Japonica populations in China
    Scientific Reports, 2017
    Co-Authors: Jie Zhang, Xiuliang Wang, Norishige Yotsukura, Tatiana N. Krupnova, Jian-ting Yao, Fuli Liu, Delin Duan
    Abstract:

    Saccharina Japonica is a commercially and ecologically important seaweed and is an excellent system for understanding the effects of domestication on marine crops. In this study, we used 19 selected simple sequence repeat (SSR) markers to investigate the influence of domestication on the genetic diversity and structure of S. Japonica populations. Wild kelp populations exhibited higher genetic diversity than cultivated populations based on total NA, HE, HO, NP and AR. Discriminant analysis of principal components (DAPC), a neighbour-joining (NJ) tree and STRUCTURE analyses indicated that S. Japonica populations could be divided into two groups (a cultivated/introduced group and a wild indigenous group) with significant genetic differentiation (P 

Xiuliang Wang - One of the best experts on this subject based on the ideXlab platform.

  • Status of genetic studies and breeding of Saccharina Japonica in China
    Journal of Oceanology and Limnology, 2020
    Co-Authors: Xiuliang Wang, Jie Zhang, Jian-ting Yao, Delin Duan
    Abstract:

    Saccharina Japonica is one of the most important economic brown seaweeds. It is intensively cultivated on large scales in a number of Asian countries. The current annual, global production is about 8 million tons valued as about 4 million US dollars. Considerable efforts have been made to S. Japonica in China since the 1950s on its cultivation. To further advance the cultivation of this species, detailed research of genetics and breeding studies are required. Recently, with the advancement of sequencing techniques, the genomics and comparative transcriptomics data were yielded, and quantitative trait locus (QTL) mapping has been conducted, along with genetic linkage maps constructed to this species. New strains have been bred and selected, with better characteristics, e.g. higher seawater temperature resistances and higher yields. In this review, we present the current status of genetic and breeding studies that have been performed to S. Japonica in China, and provide guidelines for future developments in the areas of genetic selection and breeding for this species.

  • Transcriptome sequencing of Saccharina Japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis
    2019
    Co-Authors: Zhanru Shao(former Corresponding Author), Xiuliang Wang, Zhihang Chen, Pengyan Zhang, Delin Duan(new Corresponding Author)
    Abstract:

    Abstract Background: Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in the brown seaweed Saccharina Japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown. Results: With WGCNA and trend analysis of 20,940 known genes and 4,264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and “imm upregulated 3” gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and Fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions: The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. Japonica developments. Keywords: Alginate, Mannitol, Transcriptome, Regulatory networks, Growth, Development, Saccharina Japonica

  • Transcriptome sequencing of Saccharina Japonica sporophytes during whole developmental periods reveals regulatory networks underlying alginate and mannitol biosynthesis
    2019
    Co-Authors: Zhanru Shao, Xiuliang Wang, Zhihang Chen, Pengyan Zhang, Shun Liu, Delin Duan
    Abstract:

    Abstract Background: Alginate is an important cell wall component and mannitol is a soluble storage carbon substance in brown seaweed Saccharina Japonica. Their contents vary with kelp developmental periods and harvesting time. Alginate and mannitol regulatory networks and molecular mechanisms are largely unknown.Results: With WGCNA and trend analysis of 20,940 known genes and 4,264 new genes produced from transcriptome sequencing of 30 kelp samples from different stages and tissues, we deduced that ribosomal proteins, light harvesting complex proteins and imm upregulated 3 gene family are closely associated with the meristematic growth and kelp maturity. Moreover, 134 and 6 genes directly involved in the alginate and mannitol metabolism were identified, respectively. Mannose-6-phosphate isomerase (MPI2), phosphomannomutase (PMM1), GDP-mannose 6-dehydrogenase (GMD3) and mannuronate C5-epimerase (MC5E70 and MC5E122) are closely related with the high content of alginate in the distal blade. Mannitol accumulation in the basal blade might be ascribed to high expression of mannitol-1-phosphate dehydrogenase (M1PDH1) and mannitol-1-phosphatase (M1Pase) (in biosynthesis direction) and low expression of mannitol-2-dehydrogenase (M2DH) and fructokinase (FK) (in degradation direction). Oxidative phosphorylation and photosynthesis provide ATP and NADH for mannitol metabolism whereas glycosylated cycle and tricarboxylic acid (TCA) cycle produce GTP for alginate biosynthesis. RNA/protein synthesis and transportation might affect alginate complex polymerization and secretion processes. Cryptochrome (CRY-DASH), xanthophyll cycle, photosynthesis and carbon fixation influence the production of intermediate metabolite of fructose-6-phosphate, contributing to high content of mannitol in the basal blade. Conclusions: The network of co-responsive DNA synthesis, repair and proteolysis are presumed to be involved in alginate polymerization and secretion, while upstream light-responsive reactions are important for mannitol accumulation in meristem of kelp. Our transcriptome analysis provides new insights into the transcriptional regulatory networks underlying the biosynthesis of alginate and mannitol during S. Japonica developments.Keywords: Alginate, Mannitol, Transcriptome, Regulatory networks, Growth, Development, Saccharina Japonica

  • verification of the Saccharina Japonica translocon tic20 and its localization in the chloroplast membrane in diatoms
    International Journal of Molecular Sciences, 2019
    Co-Authors: Zhihang Chen, Xiuliang Wang, Zhanru Shao, Shuang Li, Delin Duan
    Abstract:

    Tic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga Saccharina Japonica. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an “M”-type Nin-Cin-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga Phaeodactylum tricornutum, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in S. Japonica is needed.

  • Effect of domestication on the genetic diversity and structure of Saccharina Japonica populations in China
    Scientific Reports, 2017
    Co-Authors: Jie Zhang, Xiuliang Wang, Norishige Yotsukura, Tatiana N. Krupnova, Jian-ting Yao, Fuli Liu, Delin Duan
    Abstract:

    Saccharina Japonica is a commercially and ecologically important seaweed and is an excellent system for understanding the effects of domestication on marine crops. In this study, we used 19 selected simple sequence repeat (SSR) markers to investigate the influence of domestication on the genetic diversity and structure of S. Japonica populations. Wild kelp populations exhibited higher genetic diversity than cultivated populations based on total NA, HE, HO, NP and AR. Discriminant analysis of principal components (DAPC), a neighbour-joining (NJ) tree and STRUCTURE analyses indicated that S. Japonica populations could be divided into two groups (a cultivated/introduced group and a wild indigenous group) with significant genetic differentiation (P 

Jie Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina Japonica
    Journal of Applied Phycology, 2020
    Co-Authors: Jie Zhang, Norishige Yotsukura, Jian-ting Yao, Chikako Nagasato, Alexander Jueterbock, Jorge Assis, Delin Duan
    Abstract:

    Natural hybridization can play a significant role in evolutionary processes and influence the adaptive diversification and speciation of brown seaweeds. However, this phenomenon is as yet unknown in Saccharina kelps. Saccharina angustata and two varieties of Saccharina Japonica (S. Japonica var. Japonica and S. Japonica var. diabolica) partly overlap in distribution along the Pacific coast of Hokkaido, which makes them a good model system to study hybridization and introgression among species of the genus Saccharina. Based on 13 highly variable nuclear microsatellites and a mitochondrial marker, we assessed the genetic diversity levels of S. angustata for the first time and populations from Muroran to Shiranuka (western part of the Pacific coast in Hokkaido) exhibited highest genetic diversity. Genetic diversity of S. Japonica was higher in S. Japonica var. Japonica as compared with S. Japonica var. diabolica. There was significant genetic differentiation (FST > 0.25, p 

  • Status of genetic studies and breeding of Saccharina Japonica in China
    Journal of Oceanology and Limnology, 2020
    Co-Authors: Xiuliang Wang, Jie Zhang, Jian-ting Yao, Delin Duan
    Abstract:

    Saccharina Japonica is one of the most important economic brown seaweeds. It is intensively cultivated on large scales in a number of Asian countries. The current annual, global production is about 8 million tons valued as about 4 million US dollars. Considerable efforts have been made to S. Japonica in China since the 1950s on its cultivation. To further advance the cultivation of this species, detailed research of genetics and breeding studies are required. Recently, with the advancement of sequencing techniques, the genomics and comparative transcriptomics data were yielded, and quantitative trait locus (QTL) mapping has been conducted, along with genetic linkage maps constructed to this species. New strains have been bred and selected, with better characteristics, e.g. higher seawater temperature resistances and higher yields. In this review, we present the current status of genetic and breeding studies that have been performed to S. Japonica in China, and provide guidelines for future developments in the areas of genetic selection and breeding for this species.

  • Effect of domestication on the genetic diversity and structure of Saccharina Japonica populations in China
    Scientific Reports, 2017
    Co-Authors: Jie Zhang, Xiuliang Wang, Norishige Yotsukura, Tatiana N. Krupnova, Jian-ting Yao, Fuli Liu, Delin Duan
    Abstract:

    Saccharina Japonica is a commercially and ecologically important seaweed and is an excellent system for understanding the effects of domestication on marine crops. In this study, we used 19 selected simple sequence repeat (SSR) markers to investigate the influence of domestication on the genetic diversity and structure of S. Japonica populations. Wild kelp populations exhibited higher genetic diversity than cultivated populations based on total NA, HE, HO, NP and AR. Discriminant analysis of principal components (DAPC), a neighbour-joining (NJ) tree and STRUCTURE analyses indicated that S. Japonica populations could be divided into two groups (a cultivated/introduced group and a wild indigenous group) with significant genetic differentiation (P 

  • Maternal inheritance of organellar DNA demonstrated with DNA markers in crosses of Saccharina Japonica (Laminariales, Phaeophyta)
    Journal of Applied Phycology, 2015
    Co-Authors: Xiuliang Wang, Jie Zhang, Jian-ting Yao, Delin Duan
    Abstract:

    Two simple sequence repeats from chloroplast DNA (cpSSRs) and two mitochondrial DNA markers (rpl6-rps2 and trnW-trnI) were developed and used to investigate inheritance patterns of chloroplast and mitochondrial DNA in Saccharina Japonica. Haplotypes were tested in 76 progeny sporophytes from two sets of reciprocal crosses between male and female gametophytes of S. Japonica. The haplotypes were consistent with those of the maternal parents, proving that both the chloroplast and mitochondrial genomes were maternally inherited in all crosses. Forty-two cpSSRs and fourteen mtSSRs markers were developed for the present study, of which two cpSSRs and two mtSSRs proved to be polymorphic in 24 S. Japonica individuals.

  • Development of Saccharina Japonica genomic SSR markers using next-generation sequencing
    Journal of Applied Phycology, 2015
    Co-Authors: Jie Zhang, Xiuliang Wang, Jian-ting Yao, Delin Duan
    Abstract:

    Illumina HiSeq 2000 next-generation sequencing (NGS) was used on Saccharina Japonica specimens to develop SSRs from high-quality genomic sequences. There were 181,595 SSR loci identified using the MISA program. SSR repeat types are plentiful in genomic sequences of S. Japonica, with the most abundant types being mononucleotides (54 %) and trinucleotides (26 %). Six hundred primer pairs were selected and synthesized from 19,658 primer pairs, showing 166 polymorphic SSR loci (28 %) in three selected S. Japonica individuals. Sixty polymorphic SSRs were further analyzed in 24 S. Japonica individuals, showing two to seven alleles for each locus (average 3.7 alleles), with observed heterozygosities of 0~0.625 and expected heterozygosities of 0.082~0.745. Polymorphism information content (PIC) values ranged from 0.08 to 0.69. Sixteen loci were classified as informative markers (PIC > 0.5). Polymorphic SSR markers developed in this study should be useful for examining genetic diversity, assessment of population structure, QTL mapping, and marker-assisted selection in S. Japonica.

Jian-ting Yao - One of the best experts on this subject based on the ideXlab platform.

  • Detecting no natural hybridization and predicting range overlap in Saccharina angustata and Saccharina Japonica
    Journal of Applied Phycology, 2020
    Co-Authors: Jie Zhang, Norishige Yotsukura, Jian-ting Yao, Chikako Nagasato, Alexander Jueterbock, Jorge Assis, Delin Duan
    Abstract:

    Natural hybridization can play a significant role in evolutionary processes and influence the adaptive diversification and speciation of brown seaweeds. However, this phenomenon is as yet unknown in Saccharina kelps. Saccharina angustata and two varieties of Saccharina Japonica (S. Japonica var. Japonica and S. Japonica var. diabolica) partly overlap in distribution along the Pacific coast of Hokkaido, which makes them a good model system to study hybridization and introgression among species of the genus Saccharina. Based on 13 highly variable nuclear microsatellites and a mitochondrial marker, we assessed the genetic diversity levels of S. angustata for the first time and populations from Muroran to Shiranuka (western part of the Pacific coast in Hokkaido) exhibited highest genetic diversity. Genetic diversity of S. Japonica was higher in S. Japonica var. Japonica as compared with S. Japonica var. diabolica. There was significant genetic differentiation (FST > 0.25, p 

  • Status of genetic studies and breeding of Saccharina Japonica in China
    Journal of Oceanology and Limnology, 2020
    Co-Authors: Xiuliang Wang, Jie Zhang, Jian-ting Yao, Delin Duan
    Abstract:

    Saccharina Japonica is one of the most important economic brown seaweeds. It is intensively cultivated on large scales in a number of Asian countries. The current annual, global production is about 8 million tons valued as about 4 million US dollars. Considerable efforts have been made to S. Japonica in China since the 1950s on its cultivation. To further advance the cultivation of this species, detailed research of genetics and breeding studies are required. Recently, with the advancement of sequencing techniques, the genomics and comparative transcriptomics data were yielded, and quantitative trait locus (QTL) mapping has been conducted, along with genetic linkage maps constructed to this species. New strains have been bred and selected, with better characteristics, e.g. higher seawater temperature resistances and higher yields. In this review, we present the current status of genetic and breeding studies that have been performed to S. Japonica in China, and provide guidelines for future developments in the areas of genetic selection and breeding for this species.

  • Effect of domestication on the genetic diversity and structure of Saccharina Japonica populations in China
    Scientific Reports, 2017
    Co-Authors: Jie Zhang, Xiuliang Wang, Norishige Yotsukura, Tatiana N. Krupnova, Jian-ting Yao, Fuli Liu, Delin Duan
    Abstract:

    Saccharina Japonica is a commercially and ecologically important seaweed and is an excellent system for understanding the effects of domestication on marine crops. In this study, we used 19 selected simple sequence repeat (SSR) markers to investigate the influence of domestication on the genetic diversity and structure of S. Japonica populations. Wild kelp populations exhibited higher genetic diversity than cultivated populations based on total NA, HE, HO, NP and AR. Discriminant analysis of principal components (DAPC), a neighbour-joining (NJ) tree and STRUCTURE analyses indicated that S. Japonica populations could be divided into two groups (a cultivated/introduced group and a wild indigenous group) with significant genetic differentiation (P 

  • Maternal inheritance of organellar DNA demonstrated with DNA markers in crosses of Saccharina Japonica (Laminariales, Phaeophyta)
    Journal of Applied Phycology, 2015
    Co-Authors: Xiuliang Wang, Jie Zhang, Jian-ting Yao, Delin Duan
    Abstract:

    Two simple sequence repeats from chloroplast DNA (cpSSRs) and two mitochondrial DNA markers (rpl6-rps2 and trnW-trnI) were developed and used to investigate inheritance patterns of chloroplast and mitochondrial DNA in Saccharina Japonica. Haplotypes were tested in 76 progeny sporophytes from two sets of reciprocal crosses between male and female gametophytes of S. Japonica. The haplotypes were consistent with those of the maternal parents, proving that both the chloroplast and mitochondrial genomes were maternally inherited in all crosses. Forty-two cpSSRs and fourteen mtSSRs markers were developed for the present study, of which two cpSSRs and two mtSSRs proved to be polymorphic in 24 S. Japonica individuals.

  • Development of Saccharina Japonica genomic SSR markers using next-generation sequencing
    Journal of Applied Phycology, 2015
    Co-Authors: Jie Zhang, Xiuliang Wang, Jian-ting Yao, Delin Duan
    Abstract:

    Illumina HiSeq 2000 next-generation sequencing (NGS) was used on Saccharina Japonica specimens to develop SSRs from high-quality genomic sequences. There were 181,595 SSR loci identified using the MISA program. SSR repeat types are plentiful in genomic sequences of S. Japonica, with the most abundant types being mononucleotides (54 %) and trinucleotides (26 %). Six hundred primer pairs were selected and synthesized from 19,658 primer pairs, showing 166 polymorphic SSR loci (28 %) in three selected S. Japonica individuals. Sixty polymorphic SSRs were further analyzed in 24 S. Japonica individuals, showing two to seven alleles for each locus (average 3.7 alleles), with observed heterozygosities of 0~0.625 and expected heterozygosities of 0.082~0.745. Polymorphism information content (PIC) values ranged from 0.08 to 0.69. Sixteen loci were classified as informative markers (PIC > 0.5). Polymorphic SSR markers developed in this study should be useful for examining genetic diversity, assessment of population structure, QTL mapping, and marker-assisted selection in S. Japonica.

Weihua Jin - One of the best experts on this subject based on the ideXlab platform.

  • Structural Characterization and α-Glucosidase Inhibitory and Antioxidant Activities of Fucoidans Extracted from Saccharina Japonica.
    Chemistry & Biodiversity, 2020
    Co-Authors: Bin Wei, Weihua Jin, Qiu Wenhui, Tao-shun Zhou, Si-jia Wang, Jun Chen, Jianwei Chen, Huawei Zhang, Hong Wang
    Abstract:

    Two sulfated fucoidan fractions (Lj3 and Lj5) were extracted from Saccharina Japonica and then subjected to acid hydrolysis to obtain Lj3h and Lj5h. Lj3h and Lj5h were characterized using IR, methylation analysis, and mass spectrometry. It was found that Lj3h and Lj5h were homogeneous low molecular weight fucoidans. Specifically, Lj3h was composed of the main chain of 1,3-linked α-L-fucopyranose residues with sulfate at C-2 and/or C-4 and three different monosaccharides (galactose, glucose, mannose) branched at C-2 and/or C-4 of fucose residue. Lj5h contained backbones of alternating galactopyranose residues and fucopyranose residues attached via a 1→3 linkage (galactofucan) and 1→6 linked galactan. The sulfation pattern was mainly located at C2/C4 fucose or galactose residues and more branches occupied at C-4 of fucose residue and C-2, C-3 or/and C-6 of galactose residue. In vitro assay indicated that, among the four fucoidans tested, only Lj5 showed potent α-glucosidase inhibitory activity with IC50 of 153.27±22.89 μg/mL, and the two parent fucoidans, Lj3 and Lj5, showed better antioxidant activity than their derivatives. These findings highlight the structure and bioactivity diversity of Saccharina Japonica-derived fucoidans.

  • Interactions of fibroblast growth factors with sulfated galactofucan from Saccharina Japonica.
    International Journal of Biological Macromolecules, 2020
    Co-Authors: Weihua Jin, Wenjing Zhang, Di Jiang, Chunyu Wang, Ke Xia, Fuming Zhang, Robert J. Linhardt
    Abstract:

    Abstract A total 68 types of marine algae oligosaccharides and polysaccharides were prepared and used to study the structure-activity relationship of oligosaccharides and polysaccharides in their interactions with fibroblast growth factors (FGF) 1 and 2. Factors considered include different types of algae, extraction methods, molecular weight, sulfate content and fractions. In the case of low molecular weight polysaccharide (SJ-D) from Saccharina Japonica and its fractions eluting from anion exchange column, both 1.0 M NaCl fraction (SJ-D-I) and 2.0 M NaCl fraction (SJ-D-S) had stronger binding affinity than the parent SJ-D, suggesting that sulfated galactofucans represented the major tight binding component. Nuclear magnetic resonance showed that SJ-D-I was a typical sulfated galactofucan, composed of four units: 1, 3-linked 4-sulfated α-L-fucose (Fuc); 1, 3-linked 2, 4-disulfated α-L-Fuc; 1, 6-linked 4-sulfated β-D-Gal and/or 1, 6-linked 3, 4-sulfated β-D-Gal. Modification by autohydrolysis to oligosaccharides and desulfation decreased the FGF binding affinity while oversulfation increased the affinity. The solution-based affinities of SJ-D-I to FGF1 and FGF2 were 69 nM and 3.9 nM, suggesting that SJ-D-I showed better preferentially binding to FGF1 than a natural ligand, heparin, suggesting that sulfated galactofucan might represent a good regulator of FGF1.

  • Glucofucogalactan, a heterogeneous low-sulfated polysaccharide from Saccharina Japonica and its bioactivity.
    International Journal of Biological Macromolecules, 2018
    Co-Authors: Lihua Geng, Weihua Jin, Jing Wang, Quanbin Zhang, Tingting Zhao
    Abstract:

    Crude polysaccharide obtained from Saccharina Japonica using acid hydrolysis and precipitation was separated into sulfated fuco-oligosaccharide (HDF1) and heteropolysaccharide (HDF2). To further explore the bioactive fraction, HDF2 was successfully separated using membrane filtration into HDF2A and HDF2B, which differed in chemical composition and molecular weight. The bioactivity of all the fractions was tested in vitro, including immunomodulatory activity in RAW 264.7 cells and the protective activity in aristolochic acid (AA)-induced NRK-52E cell injury. HDF1 and HDF2B (low-molecular weight sulfated fucans/fuco-oligosaccharides) did not increase the nitric oxide production in RAW 264.7 cells, whereas HDF2 and HDF2A exhibited potential immunomodulatory activity. All the tested compounds showed different degrees of protective activity in AA-induced injury; HDF2A exhibited superior protective activity. Through chemical analysis, HPLC analysis, and IR spectroscopy and MS, it was determined that HDF2A was a galactose-enriched heteropolysaccharide- glucofucogalactan with a distinctive 2:1 ratio of galactose to fucose, In addition, HDF2A also contained a high amount of glucose and minor amounts of mannose, rhamnose, and xylose, with a low content of sulfate. Thus, HDF2A, a complex heterogeneous polysaccharide mixture with a unique monosaccharide composition, could be studied for further structural characterization and pharmaceutical applications. (C) 2018 Elsevier B.V. All rights reserved.

  • Comparative characterization of two GDP-mannose dehydrogenase genes from Saccharina Japonica (Laminariales, Phaeophyceae).
    BMC Plant Biology, 2016
    Co-Authors: Pengyan Zhang, Zhanru Shao, Weihua Jin, Delin Duan
    Abstract:

    Background Saccharina Japonica is an important commercial brown seaweed, its main product is alginate, which is used in food, textile and by the cosmetic and pharmaceutical industries. GDP-mannose dehydrogenase (GMD) is the key enzyme involved in the synthesis of alginate. However, little is known about GMD in S. Japonica. Here we report comparative biochemical analysis of two GMD genes in S. Japonica.

  • hypoglycemic property of acidic polysaccharide extracted from Saccharina Japonica and its potential mechanism
    Carbohydrate Polymers, 2013
    Co-Authors: Jing Wang, Weihua Jin, Wenjing Zhang, Yun Hou, Hong Zhang, Quanbin Zhang
    Abstract:

    In the present study, a sulfated polysaccharide fucoidan extracted from Saccharina Japonica was administered to normal and alloxan-diabetic rats/mice, and its effects on glycemia, insulin and serum lipid levels were evaluated. Fucoidan administered at 200 or 1200 mg/kg body weight/day could significantly reduce the blood glucose level by 22% and 34%, respectively, in alloxan-induced diabetic rats. Serum insulin levels in diabetic mice were increased by the administration of fucoidan (P<0.05). The results of an oral glucose tolerance test (OGTT) revealed that fucoidan treatment had some effect on glucose disposal after 15 days of treatment. Furthermore, fucoidan altered plasma lipid levels by lowering cholesterol, triglyceride and plasma low-density lipoprotein concentrations, while elevating plasma high-density lipoprotein cholesterol at 100 or 300 mg/kg body weight/day. The results suggested that fucoidan exhibited a considerable hypoglycemic effect, possibly by stimulating pancreatic release of insulin and/or by reducing insulin metabolism. Our results indicated that fucoidan could be developed as a potential oral hypoglycemic agents or functional food for the management of diabetes. (C) 2013 Elsevier Ltd. All rights reserved.

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