The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Yingjie Li - One of the best experts on this subject based on the ideXlab platform.
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so2 retention by highly cycled modified cao based sorbent in calcium looping process
Journal of Thermal Analysis and Calorimetry, 2014Co-Authors: Yingjie Li, Wenjing Wang, Shuimu WuAbstract:The limestone modified by pyroligneous acid has been proved to have good CO2 capture behavior in the calcium looping process. In this work, SO2 retention of the highly cycled modified limestone in the carbonation/calcination cycles was investigated in a thermogravimetric analyzer (TG). The cyclic carbonation/calcination of the modified limestone was performed in a dual fixed-bed reactor and then the cycled modified limestone was sent for Sulfation in TG. The effects of Sulfation temperature, cycle number, and prolonged carbonation on SO2 retention of the cycled modified limestone were discussed. The optimum temperature for Sulfation of the cycled modified limestone should be in the range of 900–950 °C. The effect of Sulfation temperature on SO2 retention of the modified limestone drops with increasing cycle number. With increasing cycle number from 20 to 100, the Sulfation conversion of the cycled modified limestone is stable and can reach ~0.4. The cycled modified limestone exhibits obviously higher SO2 retention than the cycled raw one for the same number of cycles. The prolonged carbonation increases SO2 retention of the modified limestone and the raw one after the subsequent cycles. The Sulfation conversions of the modified limestone and the raw one at 118 min after 9-h carbonation in the 20th cycle increase 43 and 56 %, respectively. The cycled modified limestone shows a greater SO2 retention than the cycled raw one after the same prolonged carbonation treatment. The prolonged carbonation increases the pores in 5–20 nm range which is considered the optimum pore size for Sulfation of CaO-based sorbent, so it results in an improvement in SO2 retention of the cycled sorbents.
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sequential so2 co2 capture of calcium based solid waste from the paper industry in the calcium looping process
Industrial & Engineering Chemistry Research, 2012Co-Authors: Yingjie Li, Shuimu Wu, Chunmei LuAbstract:In this work, the sequential SO 2 and CO 2 capture behavior of lime mud (LM) as a solid waste from the paper industry was investigated in the calcium looping process. In order to minimize the unfavorable effects of impurities such as Na and Cl on CO 2 and SO 2 capture of LM, the LM was prewashed with distilled water. The prewash treatment improves the cyclic CO 2 capture capacity of the LM during multiple carbonation/calcination cycles. The ultimate carbonation conversion of the treated LM is 1.8 and 4.8 times greater than those of the raw LM and the limestone, respectively. The microstructure analysis shows that the surface area and pore volume of the LM are significantly increased after the prewash treatment. With increasing the Sulfation temperature from 850 to 950 °C, both the raw LM and the treated one show an increase in the Sulfation conversion after the same number of cycles. Interestingly, the effect of the Sulfation temperature decreases with increasing the number of cycles. For the raw LM or the treated LM, the Sulfation conversion after 50 cycles is higher than that after 15 or 100 cycles. That is related to the change in pore size of the raw LM and the treated LM after multiple cycles. Compared with the raw LM and the limestone, the Sulfation conversion of the treated LM is greater after the same number of cycles and at the same reaction time.
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Sequential SO2/CO2 Capture of Calcium-Based Solid Waste from the Paper Industry in the Calcium Looping Process
Industrial & Engineering Chemistry Research, 2012Co-Authors: Yingjie Li, Shuimu Wu, Chunmei LuAbstract:In this work, the sequential SO 2 and CO 2 capture behavior of lime mud (LM) as a solid waste from the paper industry was investigated in the calcium looping process. In order to minimize the unfavorable effects of impurities such as Na and Cl on CO 2 and SO 2 capture of LM, the LM was prewashed with distilled water. The prewash treatment improves the cyclic CO 2 capture capacity of the LM during multiple carbonation/calcination cycles. The ultimate carbonation conversion of the treated LM is 1.8 and 4.8 times greater than those of the raw LM and the limestone, respectively. The microstructure analysis shows that the surface area and pore volume of the LM are significantly increased after the prewash treatment. With increasing the Sulfation temperature from 850 to 950 °C, both the raw LM and the treated one show an increase in the Sulfation conversion after the same number of cycles. Interestingly, the effect of the Sulfation temperature decreases with increasing the number of cycles. For the raw LM or the treated LM, the Sulfation conversion after 50 cycles is higher than that after 15 or 100 cycles. That is related to the change in pore size of the raw LM and the treated LM after multiple cycles. Compared with the raw LM and the limestone, the Sulfation conversion of the treated LM is greater after the same number of cycles and at the same reaction time.
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Sulfation behavior of white mud from paper manufacture as SO2 sorbent at fluidized bed combustion temperatures
Journal of Thermal Analysis and Calorimetry, 2011Co-Authors: Yingjie Li, Jianli Zhao, Chunmei LuAbstract:Abstract The calcination characteristics, Sulfation conversion, and Sulfation kinetics of a white mud from paper manufacture at fluidized bed combustion temperatures were investigated in a thermogravimetric analyzer. Also, the comparison between the white mud and the limestone in Sulfation behavior and microstructure was made. Although the white mud and the limestone both contain lots of CaCO3, they are different in the alkali metal ions content and microstructure. It results in a marked difference in Sulfation behavior between the white mud and the limestone. The CaO derived from white mud achieves the maximum Sulfation conversion of 83% at about 940 °C which is 1.7 times higher than that derived from limestone at about 880 °C. The shrinking unreacted core model is appropriate to analyze the Sulfation kinetics of the white mud. The chemical reaction activation energy Ea and the activation energy for product layer diffusion Ep for the Sulfation of the white mud are 44.94 and 55.61 kJ mol−1, respectively. ...
Chunmei Lu - One of the best experts on this subject based on the ideXlab platform.
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sequential so2 co2 capture of calcium based solid waste from the paper industry in the calcium looping process
Industrial & Engineering Chemistry Research, 2012Co-Authors: Yingjie Li, Shuimu Wu, Chunmei LuAbstract:In this work, the sequential SO 2 and CO 2 capture behavior of lime mud (LM) as a solid waste from the paper industry was investigated in the calcium looping process. In order to minimize the unfavorable effects of impurities such as Na and Cl on CO 2 and SO 2 capture of LM, the LM was prewashed with distilled water. The prewash treatment improves the cyclic CO 2 capture capacity of the LM during multiple carbonation/calcination cycles. The ultimate carbonation conversion of the treated LM is 1.8 and 4.8 times greater than those of the raw LM and the limestone, respectively. The microstructure analysis shows that the surface area and pore volume of the LM are significantly increased after the prewash treatment. With increasing the Sulfation temperature from 850 to 950 °C, both the raw LM and the treated one show an increase in the Sulfation conversion after the same number of cycles. Interestingly, the effect of the Sulfation temperature decreases with increasing the number of cycles. For the raw LM or the treated LM, the Sulfation conversion after 50 cycles is higher than that after 15 or 100 cycles. That is related to the change in pore size of the raw LM and the treated LM after multiple cycles. Compared with the raw LM and the limestone, the Sulfation conversion of the treated LM is greater after the same number of cycles and at the same reaction time.
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Sequential SO2/CO2 Capture of Calcium-Based Solid Waste from the Paper Industry in the Calcium Looping Process
Industrial & Engineering Chemistry Research, 2012Co-Authors: Yingjie Li, Shuimu Wu, Chunmei LuAbstract:In this work, the sequential SO 2 and CO 2 capture behavior of lime mud (LM) as a solid waste from the paper industry was investigated in the calcium looping process. In order to minimize the unfavorable effects of impurities such as Na and Cl on CO 2 and SO 2 capture of LM, the LM was prewashed with distilled water. The prewash treatment improves the cyclic CO 2 capture capacity of the LM during multiple carbonation/calcination cycles. The ultimate carbonation conversion of the treated LM is 1.8 and 4.8 times greater than those of the raw LM and the limestone, respectively. The microstructure analysis shows that the surface area and pore volume of the LM are significantly increased after the prewash treatment. With increasing the Sulfation temperature from 850 to 950 °C, both the raw LM and the treated one show an increase in the Sulfation conversion after the same number of cycles. Interestingly, the effect of the Sulfation temperature decreases with increasing the number of cycles. For the raw LM or the treated LM, the Sulfation conversion after 50 cycles is higher than that after 15 or 100 cycles. That is related to the change in pore size of the raw LM and the treated LM after multiple cycles. Compared with the raw LM and the limestone, the Sulfation conversion of the treated LM is greater after the same number of cycles and at the same reaction time.
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Sulfation behavior of white mud from paper manufacture as SO2 sorbent at fluidized bed combustion temperatures
Journal of Thermal Analysis and Calorimetry, 2011Co-Authors: Yingjie Li, Jianli Zhao, Chunmei LuAbstract:Abstract The calcination characteristics, Sulfation conversion, and Sulfation kinetics of a white mud from paper manufacture at fluidized bed combustion temperatures were investigated in a thermogravimetric analyzer. Also, the comparison between the white mud and the limestone in Sulfation behavior and microstructure was made. Although the white mud and the limestone both contain lots of CaCO3, they are different in the alkali metal ions content and microstructure. It results in a marked difference in Sulfation behavior between the white mud and the limestone. The CaO derived from white mud achieves the maximum Sulfation conversion of 83% at about 940 °C which is 1.7 times higher than that derived from limestone at about 880 °C. The shrinking unreacted core model is appropriate to analyze the Sulfation kinetics of the white mud. The chemical reaction activation energy Ea and the activation energy for product layer diffusion Ep for the Sulfation of the white mud are 44.94 and 55.61 kJ mol−1, respectively. ...
Shuimu Wu - One of the best experts on this subject based on the ideXlab platform.
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so2 retention by highly cycled modified cao based sorbent in calcium looping process
Journal of Thermal Analysis and Calorimetry, 2014Co-Authors: Yingjie Li, Wenjing Wang, Shuimu WuAbstract:The limestone modified by pyroligneous acid has been proved to have good CO2 capture behavior in the calcium looping process. In this work, SO2 retention of the highly cycled modified limestone in the carbonation/calcination cycles was investigated in a thermogravimetric analyzer (TG). The cyclic carbonation/calcination of the modified limestone was performed in a dual fixed-bed reactor and then the cycled modified limestone was sent for Sulfation in TG. The effects of Sulfation temperature, cycle number, and prolonged carbonation on SO2 retention of the cycled modified limestone were discussed. The optimum temperature for Sulfation of the cycled modified limestone should be in the range of 900–950 °C. The effect of Sulfation temperature on SO2 retention of the modified limestone drops with increasing cycle number. With increasing cycle number from 20 to 100, the Sulfation conversion of the cycled modified limestone is stable and can reach ~0.4. The cycled modified limestone exhibits obviously higher SO2 retention than the cycled raw one for the same number of cycles. The prolonged carbonation increases SO2 retention of the modified limestone and the raw one after the subsequent cycles. The Sulfation conversions of the modified limestone and the raw one at 118 min after 9-h carbonation in the 20th cycle increase 43 and 56 %, respectively. The cycled modified limestone shows a greater SO2 retention than the cycled raw one after the same prolonged carbonation treatment. The prolonged carbonation increases the pores in 5–20 nm range which is considered the optimum pore size for Sulfation of CaO-based sorbent, so it results in an improvement in SO2 retention of the cycled sorbents.
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sequential so2 co2 capture of calcium based solid waste from the paper industry in the calcium looping process
Industrial & Engineering Chemistry Research, 2012Co-Authors: Yingjie Li, Shuimu Wu, Chunmei LuAbstract:In this work, the sequential SO 2 and CO 2 capture behavior of lime mud (LM) as a solid waste from the paper industry was investigated in the calcium looping process. In order to minimize the unfavorable effects of impurities such as Na and Cl on CO 2 and SO 2 capture of LM, the LM was prewashed with distilled water. The prewash treatment improves the cyclic CO 2 capture capacity of the LM during multiple carbonation/calcination cycles. The ultimate carbonation conversion of the treated LM is 1.8 and 4.8 times greater than those of the raw LM and the limestone, respectively. The microstructure analysis shows that the surface area and pore volume of the LM are significantly increased after the prewash treatment. With increasing the Sulfation temperature from 850 to 950 °C, both the raw LM and the treated one show an increase in the Sulfation conversion after the same number of cycles. Interestingly, the effect of the Sulfation temperature decreases with increasing the number of cycles. For the raw LM or the treated LM, the Sulfation conversion after 50 cycles is higher than that after 15 or 100 cycles. That is related to the change in pore size of the raw LM and the treated LM after multiple cycles. Compared with the raw LM and the limestone, the Sulfation conversion of the treated LM is greater after the same number of cycles and at the same reaction time.
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Sequential SO2/CO2 Capture of Calcium-Based Solid Waste from the Paper Industry in the Calcium Looping Process
Industrial & Engineering Chemistry Research, 2012Co-Authors: Yingjie Li, Shuimu Wu, Chunmei LuAbstract:In this work, the sequential SO 2 and CO 2 capture behavior of lime mud (LM) as a solid waste from the paper industry was investigated in the calcium looping process. In order to minimize the unfavorable effects of impurities such as Na and Cl on CO 2 and SO 2 capture of LM, the LM was prewashed with distilled water. The prewash treatment improves the cyclic CO 2 capture capacity of the LM during multiple carbonation/calcination cycles. The ultimate carbonation conversion of the treated LM is 1.8 and 4.8 times greater than those of the raw LM and the limestone, respectively. The microstructure analysis shows that the surface area and pore volume of the LM are significantly increased after the prewash treatment. With increasing the Sulfation temperature from 850 to 950 °C, both the raw LM and the treated one show an increase in the Sulfation conversion after the same number of cycles. Interestingly, the effect of the Sulfation temperature decreases with increasing the number of cycles. For the raw LM or the treated LM, the Sulfation conversion after 50 cycles is higher than that after 15 or 100 cycles. That is related to the change in pore size of the raw LM and the treated LM after multiple cycles. Compared with the raw LM and the limestone, the Sulfation conversion of the treated LM is greater after the same number of cycles and at the same reaction time.
Kevin L Moore - One of the best experts on this subject based on the ideXlab platform.
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detection and purification of tyrosine sulfated proteins using a novel anti sulfotyrosine monoclonal antibody
Journal of Biological Chemistry, 2006Co-Authors: Adam J Hoffhines, Eugen Damoc, Kristie Grove Bridges, Julie A Leary, Kevin L MooreAbstract:Abstract Protein tyrosine O-Sulfation is a post-translational modification mediated by one of two Golgi tyrosylprotein sulfotransferases (TPST1 and TPST2) that catalyze the transfer of sulfate to tyrosine residues in secreted and transmembrane proteins. Tyrosine Sulfation plays a role in protein-protein interactions in several well defined systems. Although dozens of tyrosine-sulfated proteins are known, many more are likely to exist and await description. Advancing our understanding of the importance of tyrosine Sulfation in biological systems requires the development of new tools for the detection and study of tyrosine-sulfated proteins. We have developed a novel anti-sulfotyrosine monoclonal antibody (called PSG2) that binds with high affinity and exquisite specificity to sulfotyrosine residues in peptides and proteins independently of sequence context. We show that it can detect tyrosine-sulfated proteins in complex biological samples and can be used as a probe to assess the role of tyrosine Sulfation in protein function. We also demonstrate the utility of PSG2 in the purification of tyrosine-sulfated proteins from crude tissue samples. Finally, Western blot analysis using PSG2 showed that certain sperm/epididymal proteins are undersulfated in Tpst2-/- mice. This indicates that TPST1 and TPST2 have distinct macromolecular substrate specificities and provides clues as to the molecular mechanism of the infertility of Tpst2-/- males. PSG2 should be widely applicable for identification of tyrosine-sulfated proteins in other systems and organisms.
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tyrosylprotein sulfotransferase purification and molecular cloning of an enzyme that catalyzes tyrosine o Sulfation a common posttranslational modification of eukaryotic proteins
Proceedings of the National Academy of Sciences of the United States of America, 1998Co-Authors: Yingbin Ouyang, William S Lane, Kevin L MooreAbstract:Tyrosine O-Sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase (TPST) that catalyzes the transfer of sulfate from 3′-phosphoadenosine 5′-phosphosulfate to tyrosine residues within acidic motifs of polypeptides. Tyrosine O-Sulfation has been shown to be important in protein–protein interactions in several systems. For example, Sulfation of tyrosine residues in the leukocyte adhesion molecule P-selectin glycoprotein ligand 1 (PSGL-1) is required for binding to P-selectin on activated endothelium. In this report we describe the purification of TPST from rat liver microsomes based on its affinity for the N-terminal 15 amino acids of PSGL-1. We have isolated human and mouse TPST cDNAs that predict type II transmembrane proteins of 370 amino acid residues with almost identical primary structure. The human cDNA encodes a fully functional N-glycosylated enzyme with an apparent molecular mass of ≈54 kDa when expressed in mammalian cells. This enzyme defines a new class of Golgi sulfotransferases that may catalyze tyrosine O-Sulfation of PSGL-1 and other protein substrates involved in diverse physiologic functions including inflammation and hemostasis.
Jonathan W Mueller - One of the best experts on this subject based on the ideXlab platform.
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Sulfation pathways from red to green
Journal of Biological Chemistry, 2019Co-Authors: Suleyman Gunal, Rebecca Hardman, Stanislav Kopriva, Jonathan W MuellerAbstract:: Sulfur is present in the amino acids cysteine and methionine and in a large range of essential coenzymes and cofactors and is therefore essential for all organisms. It is also a constituent of sulfate esters in proteins, carbohydrates, and numerous cellular metabolites. The Sulfation and deSulfation reactions modifying a variety of different substrates are commonly known as Sulfation pathways. Although relatively little is known about the function of most sulfated metabolites, the synthesis of activated sulfate used in Sulfation pathways is essential in both animal and plant kingdoms. In humans, mutations in the genes encoding the Sulfation pathway enzymes underlie a number of developmental aberrations, and in flies and worms, their loss-of-function is fatal. In plants, a lower capacity for synthesizing activated sulfate for Sulfation reactions results in dwarfism, and a complete loss of activated sulfate synthesis is also lethal. Here, we review the similarities and differences in Sulfation pathways and associated processes in animals and plants, and we point out how they diverge from bacteria and yeast. We highlight the open questions concerning localization, regulation, and importance of Sulfation pathways in both kingdoms and the ways in which findings from these "red" and "green" experimental systems may help reciprocally address questions specific to each of the systems.
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human dhea Sulfation requires direct interaction between paps synthase 2 and dhea sulfotransferase sult2a1
Journal of Biological Chemistry, 2018Co-Authors: Rebecca Hardman, Jonathan W Mueller, Jan Idkowiak, Tarsis F Gesteira, Cecilia Vallet, Johannes Van Den Boom, Vivek Dhir, Shirley K KnauerAbstract:: The high-energy sulfate donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS), generated by human PAPS synthase isoforms PAPSS1 and PAPSS2, is required for all human Sulfation pathways. Sulfotransferase SULT2A1 uses PAPS for Sulfation of the androgen precursor dehydroepiandrosterone (DHEA), thereby reducing downstream activation of DHEA to active androgens. Human PAPSS2 mutations manifest with undetectable DHEA sulfate, androgen excess, and metabolic disease, suggesting that ubiquitous PAPSS1 cannot compensate for deficient PAPSS2 in supporting DHEA Sulfation. In knockdown studies in human adrenocortical NCI-H295R1 cells, we found that PAPSS2, but not PAPSS1, is required for efficient DHEA Sulfation. Specific APS kinase activity, the rate-limiting step in PAPS biosynthesis, did not differ between PAPSS1 and PAPSS2. Co-expression of cytoplasmic SULT2A1 with a cytoplasmic PAPSS2 variant supported DHEA Sulfation more efficiently than co-expression with nuclear PAPSS2 or nuclear/cytosolic PAPSS1. Proximity ligation assays revealed protein-protein interactions between SULT2A1 and PAPSS2 and, to a lesser extent, PAPSS1. Molecular docking studies showed a putative binding site for SULT2A1 within the PAPSS2 APS kinase domain. Energy-dependent scoring of docking solutions identified the interaction as specific for the PAPSS2 and SULT2A1 isoforms. These findings elucidate the mechanistic basis for the selective requirement for PAPSS2 in human DHEA Sulfation.
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the regulation of steroid action by Sulfation and deSulfation
Endocrine Reviews, 2015Co-Authors: Jonathan W Mueller, Jan Idkowiak, Lorna C Gilligan, Wiebke Arlt, Paul FosterAbstract:Steroid Sulfation and deSulfation are fundamental pathways vital for a functional vertebrate endocrine system. After biosynthesis, hydrophobic steroids are sulfated to expedite circulatory transit. Target cells express transmembrane organic anion-transporting polypeptides that facilitate cellular uptake of sulfated steroids. Once intracellular, sulfatases hydrolyze these steroid sulfate esters to their unconjugated, and usually active, forms. Because most steroids can be sulfated, including cholesterol, pregnenolone, dehydroepiandrosterone, and estrone, understanding the function, tissue distribution, and regulation of Sulfation and deSulfation processes provides significant insights into normal endocrine function. Not surprisingly, dysregulation of these pathways is associated with numerous pathologies, including steroid-dependent cancers, polycystic ovary syndrome, and X-linked ichthyosis. Here we provide a comprehensive examination of our current knowledge of endocrine-related Sulfation and deSulfation...
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papss2 deficiency causes androgen excess via impaired dhea Sulfation in vitro and in vivo studies in a family harboring two novel papss2 mutations
The Journal of Clinical Endocrinology and Metabolism, 2015Co-Authors: Wilma Oostdijk, Jonathan W Mueller, Jan Idkowiak, Philip J House, Angela E Taylor, Michael W Oreilly, Beverly A Hughes, Martine C De Vries, Sarina G Kant, Gijs W E SantenAbstract:Context: PAPSS2 (PAPS synthase 2) provides the universal sulfate donor PAPS (3′-phospho-adenosine-5′-phosphosulfate) to all human sulfotransferases, including SULT2A1, responsible for Sulfation of the crucial androgen precursor dehydroepiandrosterone (DHEA). Impaired DHEA Sulfation is thought to increase the conversion of DHEA toward active androgens, a proposition supported by the previous report of a girl with inactivating PAPSS2 mutations who presented with low serum DHEA sulfate and androgen excess, clinically manifesting with premature pubarche and early-onset polycystic ovary syndrome. Patients and Methods: We investigated a family harboring two novel PAPSS2 mutations, including two compound heterozygous brothers presenting with disproportionate short stature, low serum DHEA sulfate, but normal serum androgens. Patients and parents underwent a DHEA challenge test comprising frequent blood sampling and urine collection before and after 100 mg DHEA orally, with subsequent analysis of DHEA Sulfation an...
