Saccharomycopsis

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Jurgen Wendland - One of the best experts on this subject based on the ideXlab platform.

  • multi omics characterization of the necrotrophic mycoparasite Saccharomycopsis schoenii
    PLOS Pathogens, 2019
    Co-Authors: Klara Junker, Ana Hesselbart, Anna Chailyan, Jochen Forster, Jurgen Wendland
    Abstract:

    Pathogenic yeasts and fungi are an increasing global healthcare burden, but discovery of novel antifungal agents is slow. The mycoparasitic yeast Saccharomycopsis schoenii was recently demonstrated to be able to kill the emerging multi-drug resistant yeast pathogen Candida auris. However, the molecular mechanisms involved in the predatory activity of S. schoenii have not been explored. To this end, we de novo sequenced, assembled and annotated a draft genome of S. schoenii. Using proteomics, we confirmed that Saccharomycopsis yeasts have reassigned the CTG codon and translate CTG into serine instead of leucine. Further, we confirmed an absence of all genes from the sulfate assimilation pathway in the genome of S. schoenii, and detected the expansion of several gene families, including aspartic proteases. Using Saccharomyces cerevisiae as a model prey cell, we honed in on the timing and nutritional conditions under which S. schoenii kills prey cells. We found that a general nutrition limitation, not a specific methionine deficiency, triggered predatory activity. Nevertheless, by means of genome-wide transcriptome analysis we observed dramatic responses to methionine deprivation, which were alleviated when S. cerevisiae was available as prey, and therefore postulate that S. schoenii acquired methionine from its prey cells. During predation, both proteomic and transcriptomic analyses revealed that S. schoenii highly upregulated and translated aspartic protease genes, probably used to break down prey cell walls. With these fundamental insights into the predatory behavior of S. schoenii, we open up for further exploitation of this yeast as a biocontrol yeast and/or source for novel antifungal agents.

  • expansion of a telomeric flo als like sequence gene family in Saccharomycopsis fermentans
    Frontiers in Genetics, 2018
    Co-Authors: Beatrice Bernardi, Yeseren Kayacan, Jurgen Wendland
    Abstract:

    Non-Saccharomyces species have been recognized for their beneficial contribution to fermented food and beverages based on their volatile compound formation and their ability to ferment glucose into ethanol. At the end of fermentation brewer’s yeast flocculate which provides an easy means of separation of yeasts from green beer. Flocculation in Saccharomyces cerevisiae requires a set of flocculation genes. These FLO-genes, FLO1, FLO5, FLO9; FLO10 and FLO11, are located at telomeres and transcription of these adhesins is regulated by Flo8 and Mss11. Here, we show that Saccharomycopsis fermentans, an ascomycete yeast distantly related to S. cerevisiae, possesses a very large FLO/ALS-like Sequence (FAS) family encompassing 34 genes. Fas proteins are variable in size and divergent in sequence and show similarity to the Flo1/5/9 family. Fas proteins show the general build with a signal peptide, an N-terminal carbohydrate binding PA14 domain, a central region differing by the number of repeats and a C-terminus with a consensus sequence for GPI-anchor attachment. Like FLO genes in S. cerevisiae, FAS genes are mostly telomeric with several paralogs at each telomere. We term such genes that share evolutionary conserved telomere localization ‘telologs’ and provide several other examples. Adhesin expression in S. cerevisiae and filamentation in C. albicans is regulated by Flo8 and Mss11. In Saccharomycopsis we identified only a single protein with similarity to Flo8 based on sequence similarity and the presence of a LisH domain.

  • Expansion of a Telomeric FLO/ALS-Like Sequence Gene Family in Saccharomycopsis fermentans
    Frontiers in Genetics, 2018
    Co-Authors: Beatrice Bernardi, Yeseren Kayacan, Jurgen Wendland
    Abstract:

    Non-Saccharomyces species have been recognized for their beneficial contribution to fermented food and beverages based on their volatile compound formation and their ability to ferment glucose into ethanol. At the end of fermentation brewer’s yeast flocculate which provides an easy means of separation of yeasts from green beer. Flocculation in Saccharomyces cerevisiae requires a set of flocculation genes. These FLO-genes, FLO1, FLO5, FLO9; FLO10 and FLO11, are located at telomeres and transcription of these adhesins is regulated by Flo8 and Mss11. Here, we show that Saccharomycopsis fermentans, an ascomycete yeast distantly related to S. cerevisiae, possesses a very large FLO/ALS-like Sequence (FAS) family encompassing 34 genes. Fas proteins are variable in size and divergent in sequence and show similarity to the Flo1/5/9 family. Fas proteins show the general build with a signal peptide, an N-terminal carbohydrate binding PA14 domain, a central region differing by the number of repeats and a C-terminus with a consensus sequence for GPI-anchor attachment. Like FLO genes in S. cerevisiae, FAS genes are mostly telomeric with several paralogs at each telomere. We term such genes that share evolutionary conserved telomere localization ‘telologs’ and provide several other examples. Adhesin expression in S. cerevisiae and filamentation in C. albicans is regulated by Flo8 and Mss11. In Saccharomycopsis we identified only a single protein with similarity to Flo8 based on sequence similarity and the presence of a LisH domain.

  • the mycoparasitic yeast Saccharomycopsis schoenii predates and kills multi drug resistant candida auris
    Scientific Reports, 2018
    Co-Authors: Klara Junker, Gustavo Bravo Ruiz, Alexander Lorenz, Louise A Walker, Jurgen Wendland
    Abstract:

    Candida auris has recently emerged as a multi-drug resistant fungal pathogen that poses a serious global health threat, especially for patients in hospital intensive care units (ICUs). C. auris can colonize human skin and can spread by physical contact or contaminated surfaces and equipment. Here, we show that the mycoparasitic yeast Saccharomycopsis schoenii efficiently kills both sensitive and multi-drug resistant isolates of C. auris belonging to the same clade, as well as clinical isolates of other pathogenic species of the Candida genus suggesting novel approaches for biocontrol.

  • draft genome sequence of Saccharomycopsis fermentans cbs 7830 a predacious yeast belonging to the saccharomycetales
    Genome Announcements, 2018
    Co-Authors: Ana Hesselbart, Klara Junker, Jurgen Wendland
    Abstract:

    ABSTRACT Saccharomycopsis fermentans is an ascomycetous necrotrophic fungal pathogen that penetrates and kills fungal prey cells via targeted penetration pegs. Here, we report the draft genome sequence and scaffold assembly of this mycoparasite.

Santosh Nigam - One of the best experts on this subject based on the ideXlab platform.

Olihile M Sebolai - One of the best experts on this subject based on the ideXlab platform.

Klara Junker - One of the best experts on this subject based on the ideXlab platform.

  • multi omics characterization of the necrotrophic mycoparasite Saccharomycopsis schoenii
    PLOS Pathogens, 2019
    Co-Authors: Klara Junker, Ana Hesselbart, Anna Chailyan, Jochen Forster, Jurgen Wendland
    Abstract:

    Pathogenic yeasts and fungi are an increasing global healthcare burden, but discovery of novel antifungal agents is slow. The mycoparasitic yeast Saccharomycopsis schoenii was recently demonstrated to be able to kill the emerging multi-drug resistant yeast pathogen Candida auris. However, the molecular mechanisms involved in the predatory activity of S. schoenii have not been explored. To this end, we de novo sequenced, assembled and annotated a draft genome of S. schoenii. Using proteomics, we confirmed that Saccharomycopsis yeasts have reassigned the CTG codon and translate CTG into serine instead of leucine. Further, we confirmed an absence of all genes from the sulfate assimilation pathway in the genome of S. schoenii, and detected the expansion of several gene families, including aspartic proteases. Using Saccharomyces cerevisiae as a model prey cell, we honed in on the timing and nutritional conditions under which S. schoenii kills prey cells. We found that a general nutrition limitation, not a specific methionine deficiency, triggered predatory activity. Nevertheless, by means of genome-wide transcriptome analysis we observed dramatic responses to methionine deprivation, which were alleviated when S. cerevisiae was available as prey, and therefore postulate that S. schoenii acquired methionine from its prey cells. During predation, both proteomic and transcriptomic analyses revealed that S. schoenii highly upregulated and translated aspartic protease genes, probably used to break down prey cell walls. With these fundamental insights into the predatory behavior of S. schoenii, we open up for further exploitation of this yeast as a biocontrol yeast and/or source for novel antifungal agents.

  • the mycoparasitic yeast Saccharomycopsis schoenii predates and kills multi drug resistant candida auris
    Scientific Reports, 2018
    Co-Authors: Klara Junker, Gustavo Bravo Ruiz, Alexander Lorenz, Louise A Walker, Jurgen Wendland
    Abstract:

    Candida auris has recently emerged as a multi-drug resistant fungal pathogen that poses a serious global health threat, especially for patients in hospital intensive care units (ICUs). C. auris can colonize human skin and can spread by physical contact or contaminated surfaces and equipment. Here, we show that the mycoparasitic yeast Saccharomycopsis schoenii efficiently kills both sensitive and multi-drug resistant isolates of C. auris belonging to the same clade, as well as clinical isolates of other pathogenic species of the Candida genus suggesting novel approaches for biocontrol.

  • draft genome sequence of Saccharomycopsis fermentans cbs 7830 a predacious yeast belonging to the saccharomycetales
    Genome Announcements, 2018
    Co-Authors: Ana Hesselbart, Klara Junker, Jurgen Wendland
    Abstract:

    ABSTRACT Saccharomycopsis fermentans is an ascomycetous necrotrophic fungal pathogen that penetrates and kills fungal prey cells via targeted penetration pegs. Here, we report the draft genome sequence and scaffold assembly of this mycoparasite.

  • draft genome sequence of Saccharomycopsis fodiens cbs 8332 a necrotrophic mycoparasite with biocontrol potential
    Genome Announcements, 2017
    Co-Authors: Klara Junker, Ana Hesselbart, Jurgen Wendland
    Abstract:

    ABSTRACT Saccharomycopsis fodiens is an ascomycetous necrotrophic mycoparasite. Predator-prey interaction leads to killing of the host cell by a penetration peg and utilization of cell content by the predator. Here, we report the 14.9-Mb S. fodiens draft genome sequence assembled into 9 large scaffolds and 13 minor scaffolds (

Johan L F Kock - One of the best experts on this subject based on the ideXlab platform.