The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Niels Fertig - One of the best experts on this subject based on the ideXlab platform.
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state of the art automated patch clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Claudia Haarmann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and Safety Testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and Safety Testing.
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automated patch clamp with current clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Michael George, Niels FertigAbstract:Automated planar patch clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and Safety Testing. The ability to record from stem cell derived cardiomyocytes in both voltage and current clamp modes on an automated patch clamp platform is an important advancement in ion channel screening and Safety Testing. Using a planar patch clamp workstation, currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage clamp mode. In the current clamp mode, action potentials could be recorded for the first time on a planar patch clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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19 excitement over automated patch clamp action potentials from stem cells and temperature effects on herg inhibition
Heart, 2011Co-Authors: Alison Haythornthwaite, Andrea Brüggemann, Sonja Stoelzle, Claudia Haarmann, Michael George, Niels FertigAbstract:The hERG gene encodes a potassium channel responsible for the repolarisation of the IKr current in cardiac cells. Given the importance of this channel in the repolarisation of the cardiac action potential, and the disturbances of channel function by certain compounds such as anti-arrhythmias and anti-psychotics, this channel has become very important in Safety pharmacology Testing. Since some hERG-active compounds also exhibit different pharmacology at physiological temperature, experiments performed at this temperature are important in yielding more relevant data in Safety screening. In this study, we describe the use of automated patch clamp electrophysiology for recording hERG stably transfected in HEK293 cells. Recordings of the hERG current from up to eight cells simultaneously could be performed at room temperature (RT) and at physiological temperature. Data will be shown for erythromycin which exhibited a higher potency at 35°C vs RT. Additionally, using a planar patch clamp workstation, recordings could be made from stem cell-derived cardiomyocytes. Currents mediated by K + , Na + and Ca 2+ channels could be recorded in the voltage clamp mode. What is more, action potentials in the current clamp mode could also be recorded and pharmacology was performed on action potentials (data will be shown). The use of stem cell-derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both the voltage and the current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug Safety Testing. Unique features of certain planar patch clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and Safety Testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-clamp mode but also in the current-clamp mode on a planar patch clamp workstation. This demonstrates for the first time parallel current-clamp recordings on a planar patch clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the current-clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Sonja Stoelzle - One of the best experts on this subject based on the ideXlab platform.
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state of the art automated patch clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Claudia Haarmann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and Safety Testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and Safety Testing.
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automated patch clamp with current clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Michael George, Niels FertigAbstract:Automated planar patch clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and Safety Testing. The ability to record from stem cell derived cardiomyocytes in both voltage and current clamp modes on an automated patch clamp platform is an important advancement in ion channel screening and Safety Testing. Using a planar patch clamp workstation, currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage clamp mode. In the current clamp mode, action potentials could be recorded for the first time on a planar patch clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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19 excitement over automated patch clamp action potentials from stem cells and temperature effects on herg inhibition
Heart, 2011Co-Authors: Alison Haythornthwaite, Andrea Brüggemann, Sonja Stoelzle, Claudia Haarmann, Michael George, Niels FertigAbstract:The hERG gene encodes a potassium channel responsible for the repolarisation of the IKr current in cardiac cells. Given the importance of this channel in the repolarisation of the cardiac action potential, and the disturbances of channel function by certain compounds such as anti-arrhythmias and anti-psychotics, this channel has become very important in Safety pharmacology Testing. Since some hERG-active compounds also exhibit different pharmacology at physiological temperature, experiments performed at this temperature are important in yielding more relevant data in Safety screening. In this study, we describe the use of automated patch clamp electrophysiology for recording hERG stably transfected in HEK293 cells. Recordings of the hERG current from up to eight cells simultaneously could be performed at room temperature (RT) and at physiological temperature. Data will be shown for erythromycin which exhibited a higher potency at 35°C vs RT. Additionally, using a planar patch clamp workstation, recordings could be made from stem cell-derived cardiomyocytes. Currents mediated by K + , Na + and Ca 2+ channels could be recorded in the voltage clamp mode. What is more, action potentials in the current clamp mode could also be recorded and pharmacology was performed on action potentials (data will be shown). The use of stem cell-derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both the voltage and the current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug Safety Testing. Unique features of certain planar patch clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and Safety Testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-clamp mode but also in the current-clamp mode on a planar patch clamp workstation. This demonstrates for the first time parallel current-clamp recordings on a planar patch clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the current-clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Andrea Brüggemann - One of the best experts on this subject based on the ideXlab platform.
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state of the art automated patch clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Claudia Haarmann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and Safety Testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and Safety Testing.
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automated patch clamp with current clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Michael George, Niels FertigAbstract:Automated planar patch clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and Safety Testing. The ability to record from stem cell derived cardiomyocytes in both voltage and current clamp modes on an automated patch clamp platform is an important advancement in ion channel screening and Safety Testing. Using a planar patch clamp workstation, currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage clamp mode. In the current clamp mode, action potentials could be recorded for the first time on a planar patch clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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19 excitement over automated patch clamp action potentials from stem cells and temperature effects on herg inhibition
Heart, 2011Co-Authors: Alison Haythornthwaite, Andrea Brüggemann, Sonja Stoelzle, Claudia Haarmann, Michael George, Niels FertigAbstract:The hERG gene encodes a potassium channel responsible for the repolarisation of the IKr current in cardiac cells. Given the importance of this channel in the repolarisation of the cardiac action potential, and the disturbances of channel function by certain compounds such as anti-arrhythmias and anti-psychotics, this channel has become very important in Safety pharmacology Testing. Since some hERG-active compounds also exhibit different pharmacology at physiological temperature, experiments performed at this temperature are important in yielding more relevant data in Safety screening. In this study, we describe the use of automated patch clamp electrophysiology for recording hERG stably transfected in HEK293 cells. Recordings of the hERG current from up to eight cells simultaneously could be performed at room temperature (RT) and at physiological temperature. Data will be shown for erythromycin which exhibited a higher potency at 35°C vs RT. Additionally, using a planar patch clamp workstation, recordings could be made from stem cell-derived cardiomyocytes. Currents mediated by K + , Na + and Ca 2+ channels could be recorded in the voltage clamp mode. What is more, action potentials in the current clamp mode could also be recorded and pharmacology was performed on action potentials (data will be shown). The use of stem cell-derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both the voltage and the current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug Safety Testing. Unique features of certain planar patch clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and Safety Testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-clamp mode but also in the current-clamp mode on a planar patch clamp workstation. This demonstrates for the first time parallel current-clamp recordings on a planar patch clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the current-clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Michael George - One of the best experts on this subject based on the ideXlab platform.
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state of the art automated patch clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Claudia Haarmann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and Safety Testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and Safety Testing.
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automated patch clamp with current clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Michael George, Niels FertigAbstract:Automated planar patch clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and Safety Testing. The ability to record from stem cell derived cardiomyocytes in both voltage and current clamp modes on an automated patch clamp platform is an important advancement in ion channel screening and Safety Testing. Using a planar patch clamp workstation, currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage clamp mode. In the current clamp mode, action potentials could be recorded for the first time on a planar patch clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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19 excitement over automated patch clamp action potentials from stem cells and temperature effects on herg inhibition
Heart, 2011Co-Authors: Alison Haythornthwaite, Andrea Brüggemann, Sonja Stoelzle, Claudia Haarmann, Michael George, Niels FertigAbstract:The hERG gene encodes a potassium channel responsible for the repolarisation of the IKr current in cardiac cells. Given the importance of this channel in the repolarisation of the cardiac action potential, and the disturbances of channel function by certain compounds such as anti-arrhythmias and anti-psychotics, this channel has become very important in Safety pharmacology Testing. Since some hERG-active compounds also exhibit different pharmacology at physiological temperature, experiments performed at this temperature are important in yielding more relevant data in Safety screening. In this study, we describe the use of automated patch clamp electrophysiology for recording hERG stably transfected in HEK293 cells. Recordings of the hERG current from up to eight cells simultaneously could be performed at room temperature (RT) and at physiological temperature. Data will be shown for erythromycin which exhibited a higher potency at 35°C vs RT. Additionally, using a planar patch clamp workstation, recordings could be made from stem cell-derived cardiomyocytes. Currents mediated by K + , Na + and Ca 2+ channels could be recorded in the voltage clamp mode. What is more, action potentials in the current clamp mode could also be recorded and pharmacology was performed on action potentials (data will be shown). The use of stem cell-derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both the voltage and the current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug Safety Testing. Unique features of certain planar patch clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and Safety Testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-clamp mode but also in the current-clamp mode on a planar patch clamp workstation. This demonstrates for the first time parallel current-clamp recordings on a planar patch clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the current-clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Claudia Haarmann - One of the best experts on this subject based on the ideXlab platform.
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state of the art automated patch clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Claudia Haarmann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the current clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the current clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage clamp and current clamp modes with the view to finding new drug candidates and Safety Testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and Safety Testing.
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automated patch clamp with current clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Michael George, Niels FertigAbstract:Automated planar patch clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and Safety Testing. The ability to record from stem cell derived cardiomyocytes in both voltage and current clamp modes on an automated patch clamp platform is an important advancement in ion channel screening and Safety Testing. Using a planar patch clamp workstation, currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage clamp mode. In the current clamp mode, action potentials could be recorded for the first time on a planar patch clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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19 excitement over automated patch clamp action potentials from stem cells and temperature effects on herg inhibition
Heart, 2011Co-Authors: Alison Haythornthwaite, Andrea Brüggemann, Sonja Stoelzle, Claudia Haarmann, Michael George, Niels FertigAbstract:The hERG gene encodes a potassium channel responsible for the repolarisation of the IKr current in cardiac cells. Given the importance of this channel in the repolarisation of the cardiac action potential, and the disturbances of channel function by certain compounds such as anti-arrhythmias and anti-psychotics, this channel has become very important in Safety pharmacology Testing. Since some hERG-active compounds also exhibit different pharmacology at physiological temperature, experiments performed at this temperature are important in yielding more relevant data in Safety screening. In this study, we describe the use of automated patch clamp electrophysiology for recording hERG stably transfected in HEK293 cells. Recordings of the hERG current from up to eight cells simultaneously could be performed at room temperature (RT) and at physiological temperature. Data will be shown for erythromycin which exhibited a higher potency at 35°C vs RT. Additionally, using a planar patch clamp workstation, recordings could be made from stem cell-derived cardiomyocytes. Currents mediated by K + , Na + and Ca 2+ channels could be recorded in the voltage clamp mode. What is more, action potentials in the current clamp mode could also be recorded and pharmacology was performed on action potentials (data will be shown). The use of stem cell-derived cardiomyocytes in Safety Testing is becoming increasingly important. The ability to test compounds on ion channels in both the voltage and the current clamp modes, as well as at different temperatures, may be crucial for future Safety Testing.
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Andrea Brüggemann, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug Safety Testing. Unique features of certain planar patch clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and Safety Testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-clamp mode but also in the current-clamp mode on a planar patch clamp workstation. This demonstrates for the first time parallel current-clamp recordings on a planar patch clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the current-clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
