The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Christoph M. Schempp - One of the best experts on this subject based on the ideXlab platform.
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Salicin from willow bark can modulate neurite outgrowth in human neuroblastoma sh sy5y cells
Phytotherapy Research, 2015Co-Authors: Ute Wölfle, Birgit Haarhaus, Astrid Kersten, Bernd L. Fiebich, Martin J. Hug, Christoph M. SchemppAbstract:Salicin from willow bark has been used throughout centuries in China and Europe for the treatment of pain, headache, and inflammatory conditions. Recently, it could be demonstrated that Salicin binds and activates the bitter taste receptor TAS2R16. Studies on rodent tissues showed the general expression of bitter taste receptors (TAS2Rs) in rodent brain. Here, we demonstrate the expression of hTAS2R16 in human neuronal tissues and the neuroblastoma cell line SH-SY5Y. The functionality was analyzed in the neuroblastoma cell line SH-SY5Y after stimulation with Salicin, a known TAS2R16 agonist. In this setting Salicin induced in SH-SY5Y cells phosphorylation of ERK and CREB, the key transcription factor of neuronal differentiation. PD98059, an inhibitor of the ERK pathway, as well as probenecid, a TAS2R16 antagonist, inhibited receptor phosphorylation as well as neurite outgrowth. These data show that Salicin might modulate neurite outgrowth by bitter taste receptor activation. Copyright © 2015 John Wiley & Sons, Ltd.
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Salicin from Willow Bark can Modulate Neurite Outgrowth in Human Neuroblastoma SH-SY5Y Cells.
Phytotherapy research : PTR, 2015Co-Authors: Ute Wölfle, Birgit Haarhaus, Astrid Kersten, Bernd L. Fiebich, Martin J. Hug, Christoph M. SchemppAbstract:Salicin from willow bark has been used throughout centuries in China and Europe for the treatment of pain, headache, and inflammatory conditions. Recently, it could be demonstrated that Salicin binds and activates the bitter taste receptor TAS2R16. Studies on rodent tissues showed the general expression of bitter taste receptors (TAS2Rs) in rodent brain. Here, we demonstrate the expression of hTAS2R16 in human neuronal tissues and the neuroblastoma cell line SH-SY5Y. The functionality was analyzed in the neuroblastoma cell line SH-SY5Y after stimulation with Salicin, a known TAS2R16 agonist. In this setting Salicin induced in SH-SY5Y cells phosphorylation of ERK and CREB, the key transcription factor of neuronal differentiation. PD98059, an inhibitor of the ERK pathway, as well as probenecid, a TAS2R16 antagonist, inhibited receptor phosphorylation as well as neurite outgrowth. These data show that Salicin might modulate neurite outgrowth by bitter taste receptor activation.
Lih-sheng Turng - One of the best experts on this subject based on the ideXlab platform.
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expanded polytetrafluoroethylene silk fibroin Salicin vascular graft fabrication for improved endothelialization and anticoagulation
Applied Surface Science, 2021Co-Authors: Shujie Yan, Yongchao Jiang, Dongfang Wang, Xiang Zhang, Lih-sheng TurngAbstract:Abstract Clinical application of small-diameter artificial vascular grafts has been hampered by intimal blockages owing to the lack of an endothelial layer and the formation of thrombosis. In this study, an expanded polytetrafluoroethylene (ePTFE) vascular graft was fabricated using a biofriendly ethanol/water mixture as a lubricant and drug carrier. Natural silk fibroin (SF) hydrogel and white willow extract Salicin were embedded into ePTFE graft successfully via the ethanol/water lubricant and drug carrier as verified by Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The ePTFE graft functionalized by SF hydrogel improved the proliferation of human umbilical vein endothelial cell (HUVEC) due to SF’s hydrophilia and cytocompatibility as measured by the live/dead and cell counting kit-8 (CCK-8) assays. The rapid endothelialization of ePTFE graft helped reduced intimal blockages in vitro. Furthermore, the Salicin-modified ePTFE graft exhibited an anticoagulation property as demonstrated by platelet adhesion and activated partial thromboplastin time (APTT) tests. These results suggest that ePTFE graft fabricated with silk fibroin and Salicin can be an attractive candidate for vascular grafts.
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Expanded polytetrafluoroethylene/silk fibroin/Salicin vascular graft fabrication for improved endothelialization and anticoagulation
Applied Surface Science, 2021Co-Authors: Shujie Yan, Yongchao Jiang, Dongfang Wang, Xiang Zhang, Lih-sheng TurngAbstract:Abstract Clinical application of small-diameter artificial vascular grafts has been hampered by intimal blockages owing to the lack of an endothelial layer and the formation of thrombosis. In this study, an expanded polytetrafluoroethylene (ePTFE) vascular graft was fabricated using a biofriendly ethanol/water mixture as a lubricant and drug carrier. Natural silk fibroin (SF) hydrogel and white willow extract Salicin were embedded into ePTFE graft successfully via the ethanol/water lubricant and drug carrier as verified by Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The ePTFE graft functionalized by SF hydrogel improved the proliferation of human umbilical vein endothelial cell (HUVEC) due to SF’s hydrophilia and cytocompatibility as measured by the live/dead and cell counting kit-8 (CCK-8) assays. The rapid endothelialization of ePTFE graft helped reduced intimal blockages in vitro. Furthermore, the Salicin-modified ePTFE graft exhibited an anticoagulation property as demonstrated by platelet adhesion and activated partial thromboplastin time (APTT) tests. These results suggest that ePTFE graft fabricated with silk fibroin and Salicin can be an attractive candidate for vascular grafts.
Ute Wölfle - One of the best experts on this subject based on the ideXlab platform.
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Salicin from willow bark can modulate neurite outgrowth in human neuroblastoma sh sy5y cells
Phytotherapy Research, 2015Co-Authors: Ute Wölfle, Birgit Haarhaus, Astrid Kersten, Bernd L. Fiebich, Martin J. Hug, Christoph M. SchemppAbstract:Salicin from willow bark has been used throughout centuries in China and Europe for the treatment of pain, headache, and inflammatory conditions. Recently, it could be demonstrated that Salicin binds and activates the bitter taste receptor TAS2R16. Studies on rodent tissues showed the general expression of bitter taste receptors (TAS2Rs) in rodent brain. Here, we demonstrate the expression of hTAS2R16 in human neuronal tissues and the neuroblastoma cell line SH-SY5Y. The functionality was analyzed in the neuroblastoma cell line SH-SY5Y after stimulation with Salicin, a known TAS2R16 agonist. In this setting Salicin induced in SH-SY5Y cells phosphorylation of ERK and CREB, the key transcription factor of neuronal differentiation. PD98059, an inhibitor of the ERK pathway, as well as probenecid, a TAS2R16 antagonist, inhibited receptor phosphorylation as well as neurite outgrowth. These data show that Salicin might modulate neurite outgrowth by bitter taste receptor activation. Copyright © 2015 John Wiley & Sons, Ltd.
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Salicin from Willow Bark can Modulate Neurite Outgrowth in Human Neuroblastoma SH-SY5Y Cells.
Phytotherapy research : PTR, 2015Co-Authors: Ute Wölfle, Birgit Haarhaus, Astrid Kersten, Bernd L. Fiebich, Martin J. Hug, Christoph M. SchemppAbstract:Salicin from willow bark has been used throughout centuries in China and Europe for the treatment of pain, headache, and inflammatory conditions. Recently, it could be demonstrated that Salicin binds and activates the bitter taste receptor TAS2R16. Studies on rodent tissues showed the general expression of bitter taste receptors (TAS2Rs) in rodent brain. Here, we demonstrate the expression of hTAS2R16 in human neuronal tissues and the neuroblastoma cell line SH-SY5Y. The functionality was analyzed in the neuroblastoma cell line SH-SY5Y after stimulation with Salicin, a known TAS2R16 agonist. In this setting Salicin induced in SH-SY5Y cells phosphorylation of ERK and CREB, the key transcription factor of neuronal differentiation. PD98059, an inhibitor of the ERK pathway, as well as probenecid, a TAS2R16 antagonist, inhibited receptor phosphorylation as well as neurite outgrowth. These data show that Salicin might modulate neurite outgrowth by bitter taste receptor activation.
Shujie Yan - One of the best experts on this subject based on the ideXlab platform.
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expanded polytetrafluoroethylene silk fibroin Salicin vascular graft fabrication for improved endothelialization and anticoagulation
Applied Surface Science, 2021Co-Authors: Shujie Yan, Yongchao Jiang, Dongfang Wang, Xiang Zhang, Lih-sheng TurngAbstract:Abstract Clinical application of small-diameter artificial vascular grafts has been hampered by intimal blockages owing to the lack of an endothelial layer and the formation of thrombosis. In this study, an expanded polytetrafluoroethylene (ePTFE) vascular graft was fabricated using a biofriendly ethanol/water mixture as a lubricant and drug carrier. Natural silk fibroin (SF) hydrogel and white willow extract Salicin were embedded into ePTFE graft successfully via the ethanol/water lubricant and drug carrier as verified by Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The ePTFE graft functionalized by SF hydrogel improved the proliferation of human umbilical vein endothelial cell (HUVEC) due to SF’s hydrophilia and cytocompatibility as measured by the live/dead and cell counting kit-8 (CCK-8) assays. The rapid endothelialization of ePTFE graft helped reduced intimal blockages in vitro. Furthermore, the Salicin-modified ePTFE graft exhibited an anticoagulation property as demonstrated by platelet adhesion and activated partial thromboplastin time (APTT) tests. These results suggest that ePTFE graft fabricated with silk fibroin and Salicin can be an attractive candidate for vascular grafts.
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Expanded polytetrafluoroethylene/silk fibroin/Salicin vascular graft fabrication for improved endothelialization and anticoagulation
Applied Surface Science, 2021Co-Authors: Shujie Yan, Yongchao Jiang, Dongfang Wang, Xiang Zhang, Lih-sheng TurngAbstract:Abstract Clinical application of small-diameter artificial vascular grafts has been hampered by intimal blockages owing to the lack of an endothelial layer and the formation of thrombosis. In this study, an expanded polytetrafluoroethylene (ePTFE) vascular graft was fabricated using a biofriendly ethanol/water mixture as a lubricant and drug carrier. Natural silk fibroin (SF) hydrogel and white willow extract Salicin were embedded into ePTFE graft successfully via the ethanol/water lubricant and drug carrier as verified by Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). The ePTFE graft functionalized by SF hydrogel improved the proliferation of human umbilical vein endothelial cell (HUVEC) due to SF’s hydrophilia and cytocompatibility as measured by the live/dead and cell counting kit-8 (CCK-8) assays. The rapid endothelialization of ePTFE graft helped reduced intimal blockages in vitro. Furthermore, the Salicin-modified ePTFE graft exhibited an anticoagulation property as demonstrated by platelet adhesion and activated partial thromboplastin time (APTT) tests. These results suggest that ePTFE graft fabricated with silk fibroin and Salicin can be an attractive candidate for vascular grafts.
Hermínio P. Diogo - One of the best experts on this subject based on the ideXlab platform.
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calorimetric studies on the phenolic glycoside d Salicin
Journal of Pharmaceutical Sciences, 2008Co-Authors: Susana S. Pinto, Hermínio P. DiogoAbstract:A pure orthorhombic phase sample of D(-)-Salicin was purified and characterized for calorimetric measurements. From differential scanning calorimetry (DSC) measurements it was found that the onset and maximum temperatures of the fusion peak were T(on) = (473.30 +/- 0.05) K and T(max) = (474.74 +/- 0.05) K, respectively, and that the corresponding standard enthalpy of fusion was Delta(cr)(l) H(m)(o) = (55.5 +/- 0.4) kJ mol(-1). From the last two values the standard entropy of fusion is calculated as Delta(cr)(l) S(m)(o) = (116.9 +/- 0.4) J mol(-1) K(-1). The standard molar enthalpy of formation of orthorhombic D(-)-Salicin at T = 298.15 K, was determined as Delta(f) H(m)(o) (C(13)H(18)O(7), cr, orthorhombic) = -(1366.9 +/- 3.2) kJ mol(-1), by combustion calorimetry. From the results of solution calorimetry obtained in this work and some auxiliary values taken from the literature the enthalpy of reaction of hydrolysis of D(-)-Salicin to produce beta-glucose and o-hydroxybenzyl alcohol was found marginally thermoneutral, if the uncertainty interval was considered. Additionally, specific heat capacity measurements on the orthorhombic phase, glass and liquid-quenched glass obtained by DSC was reported.
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relaxation behaviour of d Salicin as studied by thermally stimulated depolarisation currents tsdc and differential scanning calorimetry dsc
International Journal of Pharmaceutics, 2008Co-Authors: Hermínio P. Diogo, Susana S. Pinto, Joaquim Moura J RamosAbstract:Abstract Thermally Stimulated Depolarisation Currents (TSDC) measurements on d (−)-Salicin have been carried out in the temperature region from −165 °C up to 150 °C. The slow molecular mobility was characterised in the crystal and in the glassy state. The value of the steepness index or fragility ( T g —normalized temperature dependence of the relaxation time) was obtained by Differential Scanning Calorimetry (DSC) from the analysis of the scanning rate dependency of T g . The existence of an unknown polymorph of Salicin is also reported.
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Relaxation behaviour of D(-)-Salicin as studied by Thermally Stimulated Depolarisation Currents (TSDC) and Differential Scanning Calorimetry (DSC).
International journal of pharmaceutics, 2008Co-Authors: Hermínio P. Diogo, Susana S. Pinto, Joaquim J. Moura RamosAbstract:Thermally Stimulated Depolarisation Currents (TSDC) measurements on D(-)-Salicin have been carried out in the temperature region from -165 degrees C up to 150 degrees C. The slow molecular mobility was characterised in the crystal and in the glassy state. The value of the steepness index or fragility (T(g)-normalized temperature dependence of the relaxation time) was obtained by Differential Scanning Calorimetry (DSC) from the analysis of the scanning rate dependency of T(g). The existence of an unknown polymorph of Salicin is also reported.
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Calorimetric Studies on the Phenolic Glycoside D(–)-Salicin
Journal of pharmaceutical sciences, 2008Co-Authors: Susana S. Pinto, Hermínio P. DiogoAbstract:A pure orthorhombic phase sample of D(-)-Salicin was purified and characterized for calorimetric measurements. From differential scanning calorimetry (DSC) measurements it was found that the onset and maximum temperatures of the fusion peak were T(on) = (473.30 +/- 0.05) K and T(max) = (474.74 +/- 0.05) K, respectively, and that the corresponding standard enthalpy of fusion was Delta(cr)(l) H(m)(o) = (55.5 +/- 0.4) kJ mol(-1). From the last two values the standard entropy of fusion is calculated as Delta(cr)(l) S(m)(o) = (116.9 +/- 0.4) J mol(-1) K(-1). The standard molar enthalpy of formation of orthorhombic D(-)-Salicin at T = 298.15 K, was determined as Delta(f) H(m)(o) (C(13)H(18)O(7), cr, orthorhombic) = -(1366.9 +/- 3.2) kJ mol(-1), by combustion calorimetry. From the results of solution calorimetry obtained in this work and some auxiliary values taken from the literature the enthalpy of reaction of hydrolysis of D(-)-Salicin to produce beta-glucose and o-hydroxybenzyl alcohol was found marginally thermoneutral, if the uncertainty interval was considered. Additionally, specific heat capacity measurements on the orthorhombic phase, glass and liquid-quenched glass obtained by DSC was reported.
