The Experts below are selected from a list of 3972 Experts worldwide ranked by ideXlab platform
Yibin Zhuang - One of the best experts on this subject based on the ideXlab platform.
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metabolic engineering of saccharomyces cerevisiae for high level production of Salidroside from glucose
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jingjie Jiang, Shuai Wang, Yibin ZhuangAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale pro...
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metabolic engineering of saccharomyces cerevisiae for high level production of Salidroside from glucose
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jingjie Jiang, Shuai Wang, Yibin ZhuangAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale pro...
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Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Salidroside from Glucose
2018Co-Authors: Jingjie Jiang, Yibin Zhuang, Shuai Wang, Hua Yin, Shaowei Liu, Tao LiuAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale production of Salidroside and tyrosol from S. cerevisiae
Shuai Wang - One of the best experts on this subject based on the ideXlab platform.
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metabolic engineering of saccharomyces cerevisiae for high level production of Salidroside from glucose
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jingjie Jiang, Shuai Wang, Yibin ZhuangAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale pro...
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metabolic engineering of saccharomyces cerevisiae for high level production of Salidroside from glucose
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jingjie Jiang, Shuai Wang, Yibin ZhuangAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale pro...
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Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Salidroside from Glucose
2018Co-Authors: Jingjie Jiang, Yibin Zhuang, Shuai Wang, Hua Yin, Shaowei Liu, Tao LiuAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale production of Salidroside and tyrosol from S. cerevisiae
Jianzong Chen - One of the best experts on this subject based on the ideXlab platform.
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Salidroside protects against mpp induced apoptosis in pc12 cells by inhibiting the no pathway
Brain Research, 2011Co-Authors: Xin Sun, Qiang Liang, Lizhen Tao, Xiaogang Kang, Jianzong ChenAbstract:Oxidative stress plays an important role in Parkinson's disease and other neurodegenerative disorders. Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L., has potent antioxidant properties. In the present study, we investigated the protective activity of Salidroside against 1-methyl-4-phenylpyridinium (MPP(+))-induced apoptosis in PC12 cells. We found that incubation of PC12 cells with Salidroside prior to MPP(+) exposure significantly reduced cell apoptosis and attenuated collapse of the mitochondrial membrane potential (MMP). Furthermore, Salidroside inhibited the MPP(+)-induced nitric oxide (NO) increase and overexpression of nNOS and iNOS and suppressed accumulation of reactive oxygen species (ROS) and intracellular free Ca(2+). Our results show that the protective effects of Salidroside on PC12 cells are mediated, at least in part, by inhibition of the NO pathway.
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Salidroside protects cardiomyocyte against hypoxia induced death a hif 1α activated and vegf mediated pathway
European Journal of Pharmacology, 2009Co-Authors: Jinping Zhang, Anheng Liu, Rongrong Hou, Juan Zhang, Xin Jia, Weifeng Jiang, Jianzong ChenAbstract:Cardiomyocyte death (necrosis and apoptosis) plays a critical role in the progress of heart diseases. Salidroside, a phenylpropanoid glycoside isolated from Rhodiola rosea L, has shown cardioprotective effects in vivo. However, whether Salidroside has a protective effect against cardiomyocyte death is poorly understood. The present study was aimed to investigate the cardioprotective role of Salidroside and the underlying mechanisms in hypoxia-induced cardiomyocyte death. Cardiomyocytes pretreated with or without Salidroside for 24 h were exposed to hypoxic condition for 6 h and then cell viability, necrosis, apoptosis, the expressions of HIF-1alpha and VEGF were investigated. Pretreatment with Salidroside markedly attenuated hypoxia-induced cell viability loss, cell necrosis and apoptosis in a dose-dependent manner. Mechanistically, pretreatment with Salidroside up-regulated the HIF-1alpha protein expression and induced its translocation. Moreover, the level of VEGF, a downstream target of HIF, was significantly increased in parallel with the level of HIF-1alpha following pretreatment with Salidroside. However, 2-methoxyestradiol (2-ME2), a HIF-1alpha inhibitor, attenuated the protection of Salidroside and blocked the increase of HIF-1alpha and VEGF. These data indicated that Salidroside has protective effect against hypoxia-induced cardiomyocytes necrosis and apoptosis by increasing HIF-1alpha expression and subsequently up-regulating VEGF levels.
Zhen Wang - One of the best experts on this subject based on the ideXlab platform.
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Salidroside protects human fibroblast cells from premature senescence induced by h2o2 partly through modulating oxidative status
Mechanisms of Ageing and Development, 2010Co-Authors: Genxiang Mao, Yan Wang, Qiang Qiu, Hongbin Deng, Longguo Yuan, Danqing Song, Zhen WangAbstract:Although Salidroside and Salidroside-like compounds are considered as most critical constitutes needed and responsible for multiple therapeutic benefits of Rhodiola rosea L., including anti-aging, direct demonstration regarding the role of Salidroside in anti-aging process is still deficient. In this study, we selected the H(2)O(2)-induced premature senescence model in human fetal lung diploid fibroblasts to investigate the protection of Salidroside against aging in vitro and associated molecular mechanisms. We found that Salidroside considerably reversed senescence-like phenotypes in the oxidant challenged model, including alterations of morphology, cell cycle, SA-β-gal staining, DNA damage, as well as related molecules expression such as p53, p21 and p16. The protection occurred in a dose-dependent manner, with 5μM offering best efficacy. The proposed antioxidant property of the compound was confirmed in this cellular system, and thus at least partially accounted for the protection of the compound against premature senescence. Similar protection of Salidroside against replicative senescence was observed as well. Interestingly, the regulation of senescence-related molecules by Salidroside involved ROS-irrelevant mechanisms in both models. This finding presents Salidroside as an attractive agent with potential to retard aging and attenuate age-related diseases in humans.
Jingjie Jiang - One of the best experts on this subject based on the ideXlab platform.
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metabolic engineering of saccharomyces cerevisiae for high level production of Salidroside from glucose
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jingjie Jiang, Shuai Wang, Yibin ZhuangAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale pro...
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metabolic engineering of saccharomyces cerevisiae for high level production of Salidroside from glucose
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Jingjie Jiang, Shuai Wang, Yibin ZhuangAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale pro...
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Metabolic Engineering of Saccharomyces cerevisiae for High-Level Production of Salidroside from Glucose
2018Co-Authors: Jingjie Jiang, Yibin Zhuang, Shuai Wang, Hua Yin, Shaowei Liu, Tao LiuAbstract:Salidroside is an important plant-derived aromatic compound with diverse biological properties. Because of inadequate natural resources, the supply of Salidroside is currently limited. In this work, we engineered the production of Salidroside in yeast. First, the aromatic aldehyde synthase (AAS) from Petroselinum crispum was overexpressed in Saccharomyces cerevisiae when combined with endogenous Ehrlich pathway to produce tyrosol from tyrosine. Glucosyltransferases from different resources were tested for ideal production of Salidroside in the yeast. Metabolic flux was enhanced toward tyrosine biosynthesis by overexpressing pathway genes and eliminating feedback inhibition. The pathway genes were integrated into yeast chromosome, leading to a recombinant strain that produced 239.5 mg/L Salidroside and 965.4 mg/L tyrosol. The production of Salidroside and tyrosol reached up to 732.5 and 1394.6 mg/L, respectively, by fed-batch fermentation. Our work provides an alternative way for industrial large-scale production of Salidroside and tyrosol from S. cerevisiae
