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Anup Kollanoor Johny - One of the best experts on this subject based on the ideXlab platform.
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effect of lemongrass essential oil against multidrug resistant Salmonella heidelberg and its attachment to chicken skin and meat
Poultry Science, 2021Co-Authors: Grace Dewi, Divek V. T. Nair, Sally Noll, Timothy J Johnson, Claire Peichel, Anup Kollanoor JohnyAbstract:Salmonella heidelberg (S. heidelberg) is a major pathogen implicated in foodborne outbreaks for which poultry products can serve as an epidemiological source. This study determined the efficacy of GRAS-status lemongrass essential oil (LGEO) against S. heidelberg in vitro and on the pathogen's attachment to skin and meat. At first, employing in vitro assays, the effect of LGEO on multidrug-resistant S. heidelberg multiplication and motility was examined. Biofilm inhibition and inactivation assays were also performed. The quorum-sensing modulating effect of LGEO was determined. In follow-up experiments, chicken skin or meat samples inoculated with S. heidelberg were treated with various concentrations of LGEO at different time points at simulated scalding (54°C) and chilling (4°C) temperatures. The samples were incubated, and the surviving populations of S. heidelberg were enumerated to determine if LGEO could be a potential processing aid in poultry operations. Duplicate samples were included in each treatment, and the experiments were repeated at least 3 times. Significant reductions of S. heidelberg of at least 4.0 log10 CFU/mL after 24 h in nutrient broth and poultry cecal contents was observed with 0.5% LGEO. Complete inhibition of motility, biofilm formation, and inactivation of pre-formed biofilms was observed with 0.15% LGEO (P ≤ 0.05). Concentrations of LGEO at 0.5% and 1% affected violacein production (P ≤ 0.05). On skin samples, all concentrations significantly reduced S. heidelberg by 1.2 to 3.9 log10 CFU/sample after 2 min at 54°C. We obtained a significant reduction of the pathogen in meat samples at 54°C and skin samples at 4°C with 2% LGEO. All concentrations significantly reduced S. heidelberg from the treatment water kept at 4°C and 54°C (P ≤ 0.05). In conclusion, LGEO could potentially serve as a natural antimicrobial strategy in scalding and chilling waters to reduce S. heidelberg during processing. However, additional studies are warranted before recommending its commercial use.
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Effect of caprylic acid alone or in combination with peracetic acid against multidrug-resistant Salmonella heidelberg on chicken drumsticks in a soft scalding temperature-time setup1
'Elsevier BV', 2021Co-Authors: Shijinaraj Manjankattil, Divek V. T. Nair, Sally Noll, Timothy J Johnson, Claire Peichel, Ryan B. Cox, Annie M. Donoghue, Anup Kollanoor JohnyAbstract:ABSTRACT: The antimicrobial efficacy of caprylic acid (CA), a medium-chain fatty acid, against multidrug-resistant Salmonella heidelberg (MDR SH) on chicken drumsticks in a soft-scalding temperature-time setup was investigated. Based on the standardization experiments in nutrient media and on chicken breast fillet portions, intact chicken drumsticks were spot inoculated with MDR SH and immersed in water with or without antimicrobial treatments at 54°C for 2 min. The treatments included 0.5% CA, 1% CA, 0.05% peracetic acid (PAA), 0.5% CA + 0.05% PAA, and 1.0% CA + 0.05% PAA. Additionally, the efficacy of the potential scald treatments against MDR SH survival on drumsticks for a storage period of 48 h at 4°C was determined. Furthermore, the effect of these treatments on the surface color of the drumsticks was also evaluated. Appropriate controls were included for statistical comparisons. The antimicrobial treatments resulted in a significant reduction of MDR SH on drumsticks. For the lower inoculum (∼2.5 log10 CFU/g) experiments, 0.5% CA, 1% CA, 0.05% PAA, 0.5% CA + 0.05% PAA, and 1.0% CA + 0.05% PAA resulted in 0.7-, 1.0-, 2.5-, 1.4-, and 1.5- log10 CFU/g reduction of MDR SH on drumsticks, respectively (P 0.05). Results indicate that CA could be an effective natural processing aid against MDR SH on chicken products
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effect of turkey derived beneficial bacteria lactobacillus salivarius and lactobacillus ingluviei on a multidrug resistant Salmonella heidelberg strain in turkey poults
Journal of Food Protection, 2019Co-Authors: Jijo Vazhakkattu Thomas, Divek V. T. Nair, Sally Noll, Timothy J Johnson, Carol J Cardona, Anup Kollanoor JohnyAbstract:Effects of turkey-derived beneficial bacteria Lactobacillus ingluviei UMNPBX19 and Lactobacillus salivarius UMNPBX2 on Salmonella heidelberg (SH) in turkey poults was investigated. Using in vitro studies, we determined each strain's resistance to pH 2.5 and 0.3% bile salts and their β-hemolysis activity. We also tested each strain's adherence to avian epithelial cells and exhibition of antimicrobial activity against major poultry-associated Salmonella. Moreover, using three in vivo experiments, we determined the effect of the strains in combination (LBIS) against SH in turkey poults. The treatment groups were negative control (-SH, -LBIS), SH control (+SH, -LBIS), and LBIS group (+SH, +LBIS). Supplementation of LBIS was done in drinking water throughout the study at a dose of 8 log CFU/gal. On day 7, poults were challenged with a 2011 ground turkey outbreak strain of SH at 5 × 105 CFU/mL, and the surviving pathogens were determined on day 7 postinoculation from the cecum, spleen, and liver. Both Lactobacillus strains exerted resistance to low pH and bile salts ( P < 0.05), showed adhesion to epithelial cells ( P < 0.05), but did not exhibit β-hemolysis. Cell-free culture supernatants of strains showed antimicrobial activity against Salmonella ( P < 0.05). Results from the in vivo studies revealed that LBIS significantly reduced dissemination of SH to the liver and spleen in all experiments, and colonization in the cecum in two of the three experiments (1.9- and 3.9-log CFU/g reductions), compared with the control. The results indicate that turkey-derived L. ingluviei UMNPBX19 and L. salivarius UMNPBX2 have potential beneficial effects against SH in turkeys. However, more studies to this effect are warranted.
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characterizing the antimicrobial function of a dairy originated probiotic propionibacterium freudenreichii against multidrug resistant Salmonella enterica serovar heidelberg in turkey poults
Frontiers in Microbiology, 2018Co-Authors: Divek V. T. Nair, Anup Kollanoor JohnyAbstract:Antimicrobial potential of a dairy-origin probiotic bacteria, Propionibacterium freudenreichii, against multidrug-resistant (MDR) Salmonella heidelberg (SH) in turkey poults was determined in the current study. Employing in vitro experiments, two strains (subsp.) of P. freudenreichii: P. freudenreichii freudenreichii B3523 (PF) and P. freudenreichii shermanii B4327 (PS) were tested for their ability to resist low pH (2.5) and bile salts (0.3%). In addition, the ability of the strains to adhere to and invade avian epithelial cells was determined after exposure to Propionibacterium strains followed by SH challenge. Moreover, the antibacterial activity of the strains’ cell-free culture supernatants (CFCSs) were tested against three major foodborne pathogens, including SH. Furthermore, the susceptibility of the strains to common antibiotics used for human therapy was determined. The hemolytic properties of the strains were determined in comparison to Streptococcus pyogenes, a known hemolysis-causing pathogen. Appropriate controls were kept in all studies. Using two in vivo experiments, PF was tested against SH colonization of poult ceca and dissemination to liver and spleen. The 4 treatment groups were: negative control, PF control (PFC), SH control (SC) and a test group (PFS; PF+SH). The poults in the PFC and PFS groups were inoculated with 1010 CFU ml-1 PF from day 1 through crop gavage and subsequently supplemented through drinking water. On day 7, SC and PFS groups were challenged with SH at 106 CFU ml-1, and after 7 days, cecum, liver, and spleen were collected for determining surviving SH populations. Results indicated that both PF and PS resisted pH=2.5 and 0.3% bile salts with surviving populations comparable to the control and adhered well onto the avian epithelial cell lines. The strains were susceptible to antibiotics and did not invade the epithelial cells or exhibit hemolytic properties. The CFCSs were highly bactericidal against all tested pathogens. In turkey poults, PF significantly reduced cecal colonization of SH and the dissemination of the pathogen to the liver, compared to the SH challenge controls (P<0.05). Results reveal that PF, a non-host GIT-derived probiotic, could be an antibiotic alternative to prevent the early colonization of SH in poults, improving the preharvest safety of turkeys.
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Characterizing the Antimicrobial Function of a Dairy-Originated Probiotic, Propionibacterium freudenreichii, Against Multidrug-Resistant Salmonella enterica Serovar heidelberg in Turkey Poults
Frontiers Media S.A., 2018Co-Authors: Divek V. T. Nair, Anup Kollanoor JohnyAbstract:Antimicrobial potential of a dairy-origin probiotic bacteria, Propionibacterium freudenreichii, against multidrug-resistant Salmonella heidelberg (SH) in turkey poults was determined in the current study. Employing in vitro experiments, two strains (subsp.) of P. freudenreichii: P. freudenreichii freudenreichii B3523 (PF) and P. freudenreichii shermanii B4327 (PS) were tested for their ability to resist low pH (2.5) and bile salts (0.3%). In addition, the ability of the strains to adhere to and invade avian epithelial cells was determined after exposure to Propionibacterium strains followed by SH challenge. Moreover, the antibacterial activity of the strains’ cell-free culture supernatants (CFCSs) were tested against three major foodborne pathogens, including SH. Furthermore, the susceptibility of the strains to common antibiotics used for human therapy was determined. The hemolytic properties of the strains were determined in comparison to Streptococcus pyogenes, a known hemolysis-causing pathogen. Appropriate controls were kept in all studies. Using two in vivo experiments, PF was tested against SH colonization of poult ceca and dissemination to liver and spleen. The four treatment groups were: negative control, PF control (PFC), SH control (SC), and a test group (PFS; PF + SH). The poults in the PFC and PFS groups were inoculated with 1010 CFU ml−1 PF on day 1 through crop gavage and subsequently supplemented through drinking water. On day 7, SC and PFS groups were challenged with SH at 106 CFU ml−1, and after 7 days, cecum, liver, and spleen were collected for determining surviving SH populations. Results indicated that both PF and PS resisted pH = 2.5 and 0.3% bile salts with surviving populations comparable to the control and adhered well onto the avian epithelial cell lines. The strains were susceptible to antibiotics and did not invade the epithelial cells or exhibit hemolytic properties. The CFCSs were highly bactericidal against all tested pathogens. In turkey poults, PF significantly reduced cecal colonization of SH and the dissemination of the pathogen to the liver, compared to the SH challenge controls (P < 0.05). Results revealed that PF, a non-host gastrointestinal tract-derived probiotic, could be an antibiotic alternative to prevent the early colonization of SH in poults, improving the preharvest safety of turkeys
Huaijun Zhou - One of the best experts on this subject based on the ideXlab platform.
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transcriptome analysis of Salmonella heidelberg after exposure to cetylpyridinium chloride acidified calcium hypochlorite and peroxyacetic acid
Journal of Food Protection, 2019Co-Authors: Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L Settles, Blythe Durbinjohnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun ZhouAbstract:The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0%), 28 (31.1%), and 1 (1.1%) gene were upregulated in SH 2014 and 21 (23.3%), 26 (28.9%), and 2 (2.2%) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.
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transcriptome analysis of Salmonella heidelberg after exposure to cetylpyridinium chloride acidified calcium hypochlorite and peroxyacetic acid
Journal of Food Protection, 2019Co-Authors: Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L Settles, Blythe Durbinjohnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun ZhouAbstract:ABSTRACT The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcr...
Divek V. T. Nair - One of the best experts on this subject based on the ideXlab platform.
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effect of lemongrass essential oil against multidrug resistant Salmonella heidelberg and its attachment to chicken skin and meat
Poultry Science, 2021Co-Authors: Grace Dewi, Divek V. T. Nair, Sally Noll, Timothy J Johnson, Claire Peichel, Anup Kollanoor JohnyAbstract:Salmonella heidelberg (S. heidelberg) is a major pathogen implicated in foodborne outbreaks for which poultry products can serve as an epidemiological source. This study determined the efficacy of GRAS-status lemongrass essential oil (LGEO) against S. heidelberg in vitro and on the pathogen's attachment to skin and meat. At first, employing in vitro assays, the effect of LGEO on multidrug-resistant S. heidelberg multiplication and motility was examined. Biofilm inhibition and inactivation assays were also performed. The quorum-sensing modulating effect of LGEO was determined. In follow-up experiments, chicken skin or meat samples inoculated with S. heidelberg were treated with various concentrations of LGEO at different time points at simulated scalding (54°C) and chilling (4°C) temperatures. The samples were incubated, and the surviving populations of S. heidelberg were enumerated to determine if LGEO could be a potential processing aid in poultry operations. Duplicate samples were included in each treatment, and the experiments were repeated at least 3 times. Significant reductions of S. heidelberg of at least 4.0 log10 CFU/mL after 24 h in nutrient broth and poultry cecal contents was observed with 0.5% LGEO. Complete inhibition of motility, biofilm formation, and inactivation of pre-formed biofilms was observed with 0.15% LGEO (P ≤ 0.05). Concentrations of LGEO at 0.5% and 1% affected violacein production (P ≤ 0.05). On skin samples, all concentrations significantly reduced S. heidelberg by 1.2 to 3.9 log10 CFU/sample after 2 min at 54°C. We obtained a significant reduction of the pathogen in meat samples at 54°C and skin samples at 4°C with 2% LGEO. All concentrations significantly reduced S. heidelberg from the treatment water kept at 4°C and 54°C (P ≤ 0.05). In conclusion, LGEO could potentially serve as a natural antimicrobial strategy in scalding and chilling waters to reduce S. heidelberg during processing. However, additional studies are warranted before recommending its commercial use.
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propionibacterium freudenreichii freudenreichii b3523 reduces cecal colonization and internal organ dissemination of multidrug resistant Salmonella heidelberg in finishing turkeys
The Journal of Applied Poultry Research, 2021Co-Authors: Divek V. T. Nair, Vazhakkattu J Thomas, G Dewi, J A Brannon, Sally Noll, Timothy J Johnson, R B Cox, Kollanoor A JohnyAbstract:Summary Previously, we reported that a dairy-originated probiotic bacterium, Propionibacterium freudenreichii subsp. freudenreichii B3523 (PF) reduced cecal colonization of multidrug-resistant Salmonella enterica serovar heidelberg (SH) in poults (2-wk-old) and growing (7-wk-old) turkeys. In the present study, we validated the antibacterial potential of PF in finisher (12-wk-old) turkeys using 2 separate experiments. In each experiment, day-old poults were randomly allocated to 3 treatment groups, with 10 turkeys in each group (N = 30 turkeys/experiment). The treatment groups were a negative control (turkeys without PF supplementation or SH challenge), an SH control (SC; turkeys challenged with SH without PF supplementation), and a test group (PFS; turkeys supplemented with PF and challenged with SH). Supplementation of PF at 1012 cfu/5 gallons water was provided to the turkeys in the PFS groups for 12 wk. The turkeys in the SC and PFS groups were challenged with SH at 108 cfu/turkey at 11 wk of age. Five turkeys from each group were euthanized 2 and 7 d after SH challenge. The cecum, liver, and spleen samples were collected to determine colonization of SH in the cecum and SH dissemination to the targeted internal organs. Propionibacterium freudenreichii subsp. freudenreichii B3523 was recovered at 4.0 log10 cfu/g in the turkey cecum. Supplementation of PF reduced the cecal colonization of SH by >2.0 log10 cfu/g in finishing turkeys (P
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Effect of caprylic acid alone or in combination with peracetic acid against multidrug-resistant Salmonella heidelberg on chicken drumsticks in a soft scalding temperature-time setup1
'Elsevier BV', 2021Co-Authors: Shijinaraj Manjankattil, Divek V. T. Nair, Sally Noll, Timothy J Johnson, Claire Peichel, Ryan B. Cox, Annie M. Donoghue, Anup Kollanoor JohnyAbstract:ABSTRACT: The antimicrobial efficacy of caprylic acid (CA), a medium-chain fatty acid, against multidrug-resistant Salmonella heidelberg (MDR SH) on chicken drumsticks in a soft-scalding temperature-time setup was investigated. Based on the standardization experiments in nutrient media and on chicken breast fillet portions, intact chicken drumsticks were spot inoculated with MDR SH and immersed in water with or without antimicrobial treatments at 54°C for 2 min. The treatments included 0.5% CA, 1% CA, 0.05% peracetic acid (PAA), 0.5% CA + 0.05% PAA, and 1.0% CA + 0.05% PAA. Additionally, the efficacy of the potential scald treatments against MDR SH survival on drumsticks for a storage period of 48 h at 4°C was determined. Furthermore, the effect of these treatments on the surface color of the drumsticks was also evaluated. Appropriate controls were included for statistical comparisons. The antimicrobial treatments resulted in a significant reduction of MDR SH on drumsticks. For the lower inoculum (∼2.5 log10 CFU/g) experiments, 0.5% CA, 1% CA, 0.05% PAA, 0.5% CA + 0.05% PAA, and 1.0% CA + 0.05% PAA resulted in 0.7-, 1.0-, 2.5-, 1.4-, and 1.5- log10 CFU/g reduction of MDR SH on drumsticks, respectively (P 0.05). Results indicate that CA could be an effective natural processing aid against MDR SH on chicken products
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effect of turkey derived beneficial bacteria lactobacillus salivarius and lactobacillus ingluviei on a multidrug resistant Salmonella heidelberg strain in turkey poults
Journal of Food Protection, 2019Co-Authors: Jijo Vazhakkattu Thomas, Divek V. T. Nair, Sally Noll, Timothy J Johnson, Carol J Cardona, Anup Kollanoor JohnyAbstract:Effects of turkey-derived beneficial bacteria Lactobacillus ingluviei UMNPBX19 and Lactobacillus salivarius UMNPBX2 on Salmonella heidelberg (SH) in turkey poults was investigated. Using in vitro studies, we determined each strain's resistance to pH 2.5 and 0.3% bile salts and their β-hemolysis activity. We also tested each strain's adherence to avian epithelial cells and exhibition of antimicrobial activity against major poultry-associated Salmonella. Moreover, using three in vivo experiments, we determined the effect of the strains in combination (LBIS) against SH in turkey poults. The treatment groups were negative control (-SH, -LBIS), SH control (+SH, -LBIS), and LBIS group (+SH, +LBIS). Supplementation of LBIS was done in drinking water throughout the study at a dose of 8 log CFU/gal. On day 7, poults were challenged with a 2011 ground turkey outbreak strain of SH at 5 × 105 CFU/mL, and the surviving pathogens were determined on day 7 postinoculation from the cecum, spleen, and liver. Both Lactobacillus strains exerted resistance to low pH and bile salts ( P < 0.05), showed adhesion to epithelial cells ( P < 0.05), but did not exhibit β-hemolysis. Cell-free culture supernatants of strains showed antimicrobial activity against Salmonella ( P < 0.05). Results from the in vivo studies revealed that LBIS significantly reduced dissemination of SH to the liver and spleen in all experiments, and colonization in the cecum in two of the three experiments (1.9- and 3.9-log CFU/g reductions), compared with the control. The results indicate that turkey-derived L. ingluviei UMNPBX19 and L. salivarius UMNPBX2 have potential beneficial effects against SH in turkeys. However, more studies to this effect are warranted.
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characterizing the antimicrobial function of a dairy originated probiotic propionibacterium freudenreichii against multidrug resistant Salmonella enterica serovar heidelberg in turkey poults
Frontiers in Microbiology, 2018Co-Authors: Divek V. T. Nair, Anup Kollanoor JohnyAbstract:Antimicrobial potential of a dairy-origin probiotic bacteria, Propionibacterium freudenreichii, against multidrug-resistant (MDR) Salmonella heidelberg (SH) in turkey poults was determined in the current study. Employing in vitro experiments, two strains (subsp.) of P. freudenreichii: P. freudenreichii freudenreichii B3523 (PF) and P. freudenreichii shermanii B4327 (PS) were tested for their ability to resist low pH (2.5) and bile salts (0.3%). In addition, the ability of the strains to adhere to and invade avian epithelial cells was determined after exposure to Propionibacterium strains followed by SH challenge. Moreover, the antibacterial activity of the strains’ cell-free culture supernatants (CFCSs) were tested against three major foodborne pathogens, including SH. Furthermore, the susceptibility of the strains to common antibiotics used for human therapy was determined. The hemolytic properties of the strains were determined in comparison to Streptococcus pyogenes, a known hemolysis-causing pathogen. Appropriate controls were kept in all studies. Using two in vivo experiments, PF was tested against SH colonization of poult ceca and dissemination to liver and spleen. The 4 treatment groups were: negative control, PF control (PFC), SH control (SC) and a test group (PFS; PF+SH). The poults in the PFC and PFS groups were inoculated with 1010 CFU ml-1 PF from day 1 through crop gavage and subsequently supplemented through drinking water. On day 7, SC and PFS groups were challenged with SH at 106 CFU ml-1, and after 7 days, cecum, liver, and spleen were collected for determining surviving SH populations. Results indicated that both PF and PS resisted pH=2.5 and 0.3% bile salts with surviving populations comparable to the control and adhered well onto the avian epithelial cell lines. The strains were susceptible to antibiotics and did not invade the epithelial cells or exhibit hemolytic properties. The CFCSs were highly bactericidal against all tested pathogens. In turkey poults, PF significantly reduced cecal colonization of SH and the dissemination of the pathogen to the liver, compared to the SH challenge controls (P<0.05). Results reveal that PF, a non-host GIT-derived probiotic, could be an antibiotic alternative to prevent the early colonization of SH in poults, improving the preharvest safety of turkeys.
Myrna Cadena - One of the best experts on this subject based on the ideXlab platform.
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transcriptome analysis of Salmonella heidelberg after exposure to cetylpyridinium chloride acidified calcium hypochlorite and peroxyacetic acid
Journal of Food Protection, 2019Co-Authors: Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L Settles, Blythe Durbinjohnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun ZhouAbstract:The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0%), 28 (31.1%), and 1 (1.1%) gene were upregulated in SH 2014 and 21 (23.3%), 26 (28.9%), and 2 (2.2%) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.
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transcriptome analysis of Salmonella heidelberg after exposure to cetylpyridinium chloride acidified calcium hypochlorite and peroxyacetic acid
Journal of Food Protection, 2019Co-Authors: Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L Settles, Blythe Durbinjohnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun ZhouAbstract:ABSTRACT The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcr...
Lutz Froenicke - One of the best experts on this subject based on the ideXlab platform.
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transcriptome analysis of Salmonella heidelberg after exposure to cetylpyridinium chloride acidified calcium hypochlorite and peroxyacetic acid
Journal of Food Protection, 2019Co-Authors: Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L Settles, Blythe Durbinjohnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun ZhouAbstract:The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcription of genes associated with virulence and survivability. In this study, we evaluated the effects of disinfectant exposure on the transcriptomes of two field isolates of Salmonella heidelberg (SH) isolated from a commercial broiler processing plant in 1992 and 2014. The isolates were each exposed separately to the following disinfectants commonly used in poultry processing: cetylpyridinium chloride (CPC), acidified calcium hypochlorite (aCH), and peroxyacetic acid (PAA). Exposure times were 8 s with CPC to simulate a poultry processing dipping station or 90 min with aCH and PAA to simulate the chiller tank in a poultry processing plant at 4°C. Based on comparison with a publicly available annotated SH reference genome with 5,088 genes, 90 genes were identified as associated with virulence, pathogenicity, and resistance (VPR). Of these 90 VPR genes, 9 (10.0%), 28 (31.1%), and 1 (1.1%) gene were upregulated in SH 2014 and 21 (23.3%), 26 (28.9%), and 2 (2.2%) genes were upregulated in SH 2014 challenged with CPC, aCH, and PAA, respectively. This information and previously reported MICs for the three disinfectants with both SH isolates allow researchers to make more accurate recommendations regarding control methods of SH and public health considerations related to SH in food production facilities where SH has been isolated. For example, the MICs revealed that aCH is ineffective for SH inhibition at regulatory levels allowed for poultry processing and that aCH was ineffective for inhibiting SH growth and caused an upregulation of VPR genes.
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transcriptome analysis of Salmonella heidelberg after exposure to cetylpyridinium chloride acidified calcium hypochlorite and peroxyacetic acid
Journal of Food Protection, 2019Co-Authors: Myrna Cadena, Lutz Froenicke, Monica Britton, Matthew L Settles, Blythe Durbinjohnson, Emily Kumimoto, Rodrigo A Gallardo, Aura Ferreiro, Tereza Chylkova, Huaijun ZhouAbstract:ABSTRACT The application of RNA sequencing in commercial poultry could facilitate a novel approach toward food safety with respect to identifying conditions in food production that mitigate transcr...
