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Jorge Santo W Domingo - One of the best experts on this subject based on the ideXlab platform.
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molecular detection of campylobacter spp and fecal indicator bacteria during the northern migration of Sandhill Cranes grus canadensis at the central platte river
Applied and Environmental Microbiology, 2013Co-Authors: Hodon Ryu, Jason R Vogel, Jorge Santo W Domingo, Nicholas J. AshboltAbstract:ABSTRACT The risk to human health of the annual Sandhill Crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from Crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in Sandhill Crane excreta. Campylobacter spp. were detected in 48% of Crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 i?½ 10 8 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 i?½ 10 5 CE/100 ml). Enterococcus spp., E. coli, and Campylobacter spp. were significantly increased in river water and sediments during the Crane migration period, with Enterococcus sp. densities (∼3.3 i?½ 10 5 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 i?½ 10 3 CE/g), E. coli (2.2 i?½ 10 3 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and Crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds.
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impacts of migratory Sandhill Cranes grus canadensis on microbial water quality in the central platte river nebraska usa
Water Air and Soil Pollution, 2013Co-Authors: Jason R Vogel, Hodon Ryu, Nicholas J. Ashbolt, Dale W Griffin, Matthew T Moser, Mary K Beitz, Jorge Santo W DomingoAbstract:Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of bird-derived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water samples were collected from January through May of each year during the spring migration season of Sandhill Cranes in the Central Flyway of North America. Results indicate that several types of fecal indicator bacteria and from a range of viral, protozoan, and bacterial pathogens, Campylobacter jejuni were present in Sandhill Crane excreta, and at significantly higher frequency and densities in water and sediments when the Sandhill Cranes were present, particularly during evening roosts within the Platte River environment. Therefore, further investigation of the health significance of avian pathogens is warranted for the Platte River in Central Nebraska during migration of Sandhill Cranes and other waterfowl.
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development and evaluation of a quantitative pcr assay targeting Sandhill Crane grus canadensis fecal pollution
Applied and Environmental Microbiology, 2012Co-Authors: Hodon Ryu, Nicholas J. Ashbolt, Felipe Chavezramirez, Jason R Vogel, Michael Elk, Jorge Santo W DomingoAbstract:While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating Cranes, there are no methods available to study Crane fecal contamination. Here we characterized microbial populations in Crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel Crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters. Bacteria from Crane excreta were dominated by bacilli and proteobacteria, with a notable paucity of sequences homologous to Bacteroidetes and Clostridia. The Crane1 marker targeted a dominant clade of unclassified Lactobacillales sequences closely related to Catellicoccus marimammalium. The host distribution of the Crane1 marker was relatively high, being positive for 69% (66/96) of the Crane excreta samples tested. The assay also showed high host specificity, with 95% of the nontarget fecal samples (i.e., n = 553; 20 different free-range hosts) being negative. Of the presumed Crane-impacted water samples (n = 16), 88% were positive for the Crane1 assay, whereas none of the water samples not impacted by Cranes were positive (n = 165). Bayesian statistical models of the Crane1 MST marker demonstrated high confidence in detecting true-positive signals and a low probability of false-negative signals from environmental water samples. Altogether, these data suggest that the newly developed marker could be used in environmental monitoring studies to study Crane fecal pollution dynamics.
Nicholas J. Ashbolt - One of the best experts on this subject based on the ideXlab platform.
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molecular detection of campylobacter spp and fecal indicator bacteria during the northern migration of Sandhill Cranes grus canadensis at the central platte river
Applied and Environmental Microbiology, 2013Co-Authors: Hodon Ryu, Jason R Vogel, Jorge Santo W Domingo, Nicholas J. AshboltAbstract:ABSTRACT The risk to human health of the annual Sandhill Crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from Crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in Sandhill Crane excreta. Campylobacter spp. were detected in 48% of Crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 i?½ 10 8 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 i?½ 10 5 CE/100 ml). Enterococcus spp., E. coli, and Campylobacter spp. were significantly increased in river water and sediments during the Crane migration period, with Enterococcus sp. densities (∼3.3 i?½ 10 5 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 i?½ 10 3 CE/g), E. coli (2.2 i?½ 10 3 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and Crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds.
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impacts of migratory Sandhill Cranes grus canadensis on microbial water quality in the central platte river nebraska usa
Water Air and Soil Pollution, 2013Co-Authors: Jason R Vogel, Hodon Ryu, Nicholas J. Ashbolt, Dale W Griffin, Matthew T Moser, Mary K Beitz, Jorge Santo W DomingoAbstract:Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of bird-derived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water samples were collected from January through May of each year during the spring migration season of Sandhill Cranes in the Central Flyway of North America. Results indicate that several types of fecal indicator bacteria and from a range of viral, protozoan, and bacterial pathogens, Campylobacter jejuni were present in Sandhill Crane excreta, and at significantly higher frequency and densities in water and sediments when the Sandhill Cranes were present, particularly during evening roosts within the Platte River environment. Therefore, further investigation of the health significance of avian pathogens is warranted for the Platte River in Central Nebraska during migration of Sandhill Cranes and other waterfowl.
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development and evaluation of a quantitative pcr assay targeting Sandhill Crane grus canadensis fecal pollution
Applied and Environmental Microbiology, 2012Co-Authors: Hodon Ryu, Nicholas J. Ashbolt, Felipe Chavezramirez, Jason R Vogel, Michael Elk, Jorge Santo W DomingoAbstract:While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating Cranes, there are no methods available to study Crane fecal contamination. Here we characterized microbial populations in Crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel Crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters. Bacteria from Crane excreta were dominated by bacilli and proteobacteria, with a notable paucity of sequences homologous to Bacteroidetes and Clostridia. The Crane1 marker targeted a dominant clade of unclassified Lactobacillales sequences closely related to Catellicoccus marimammalium. The host distribution of the Crane1 marker was relatively high, being positive for 69% (66/96) of the Crane excreta samples tested. The assay also showed high host specificity, with 95% of the nontarget fecal samples (i.e., n = 553; 20 different free-range hosts) being negative. Of the presumed Crane-impacted water samples (n = 16), 88% were positive for the Crane1 assay, whereas none of the water samples not impacted by Cranes were positive (n = 165). Bayesian statistical models of the Crane1 MST marker demonstrated high confidence in detecting true-positive signals and a low probability of false-negative signals from environmental water samples. Altogether, these data suggest that the newly developed marker could be used in environmental monitoring studies to study Crane fecal pollution dynamics.
Hodon Ryu - One of the best experts on this subject based on the ideXlab platform.
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molecular detection of campylobacter spp and fecal indicator bacteria during the northern migration of Sandhill Cranes grus canadensis at the central platte river
Applied and Environmental Microbiology, 2013Co-Authors: Hodon Ryu, Jason R Vogel, Jorge Santo W Domingo, Nicholas J. AshboltAbstract:ABSTRACT The risk to human health of the annual Sandhill Crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from Crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in Sandhill Crane excreta. Campylobacter spp. were detected in 48% of Crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 i?½ 10 8 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 i?½ 10 5 CE/100 ml). Enterococcus spp., E. coli, and Campylobacter spp. were significantly increased in river water and sediments during the Crane migration period, with Enterococcus sp. densities (∼3.3 i?½ 10 5 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 i?½ 10 3 CE/g), E. coli (2.2 i?½ 10 3 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and Crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds.
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impacts of migratory Sandhill Cranes grus canadensis on microbial water quality in the central platte river nebraska usa
Water Air and Soil Pollution, 2013Co-Authors: Jason R Vogel, Hodon Ryu, Nicholas J. Ashbolt, Dale W Griffin, Matthew T Moser, Mary K Beitz, Jorge Santo W DomingoAbstract:Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of bird-derived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water samples were collected from January through May of each year during the spring migration season of Sandhill Cranes in the Central Flyway of North America. Results indicate that several types of fecal indicator bacteria and from a range of viral, protozoan, and bacterial pathogens, Campylobacter jejuni were present in Sandhill Crane excreta, and at significantly higher frequency and densities in water and sediments when the Sandhill Cranes were present, particularly during evening roosts within the Platte River environment. Therefore, further investigation of the health significance of avian pathogens is warranted for the Platte River in Central Nebraska during migration of Sandhill Cranes and other waterfowl.
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development and evaluation of a quantitative pcr assay targeting Sandhill Crane grus canadensis fecal pollution
Applied and Environmental Microbiology, 2012Co-Authors: Hodon Ryu, Nicholas J. Ashbolt, Felipe Chavezramirez, Jason R Vogel, Michael Elk, Jorge Santo W DomingoAbstract:While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating Cranes, there are no methods available to study Crane fecal contamination. Here we characterized microbial populations in Crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel Crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters. Bacteria from Crane excreta were dominated by bacilli and proteobacteria, with a notable paucity of sequences homologous to Bacteroidetes and Clostridia. The Crane1 marker targeted a dominant clade of unclassified Lactobacillales sequences closely related to Catellicoccus marimammalium. The host distribution of the Crane1 marker was relatively high, being positive for 69% (66/96) of the Crane excreta samples tested. The assay also showed high host specificity, with 95% of the nontarget fecal samples (i.e., n = 553; 20 different free-range hosts) being negative. Of the presumed Crane-impacted water samples (n = 16), 88% were positive for the Crane1 assay, whereas none of the water samples not impacted by Cranes were positive (n = 165). Bayesian statistical models of the Crane1 MST marker demonstrated high confidence in detecting true-positive signals and a low probability of false-negative signals from environmental water samples. Altogether, these data suggest that the newly developed marker could be used in environmental monitoring studies to study Crane fecal pollution dynamics.
Jason R Vogel - One of the best experts on this subject based on the ideXlab platform.
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molecular detection of campylobacter spp and fecal indicator bacteria during the northern migration of Sandhill Cranes grus canadensis at the central platte river
Applied and Environmental Microbiology, 2013Co-Authors: Hodon Ryu, Jason R Vogel, Jorge Santo W Domingo, Nicholas J. AshboltAbstract:ABSTRACT The risk to human health of the annual Sandhill Crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from Crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in Sandhill Crane excreta. Campylobacter spp. were detected in 48% of Crane excreta, 24% of water samples, and 11% of sediment samples. The estimated densities of Enterococcus spp. were highest in excreta samples (mean, 4.6 i?½ 10 8 cell equivalents [CE]/g), while water samples contained higher levels of Bacteroidetes (mean, 5.1 i?½ 10 5 CE/100 ml). Enterococcus spp., E. coli, and Campylobacter spp. were significantly increased in river water and sediments during the Crane migration period, with Enterococcus sp. densities (∼3.3 i?½ 10 5 CE/g) 2 to 4 orders of magnitude higher than those of Bacteroidetes (4.9 i?½ 10 3 CE/g), E. coli (2.2 i?½ 10 3 CE/g), and Campylobacter spp. (37 CE/g). Sequencing data for the 16S rRNA gene and Campylobacter species-specific PCR assays indicated that C. jejuni was the major Campylobacter species present in water, sediments, and Crane excreta. Overall, migration appeared to result in a significant, but temporary, change in water quality in spring, when there may be a C. jejuni health hazard associated with water and crops visited by the migrating birds.
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impacts of migratory Sandhill Cranes grus canadensis on microbial water quality in the central platte river nebraska usa
Water Air and Soil Pollution, 2013Co-Authors: Jason R Vogel, Hodon Ryu, Nicholas J. Ashbolt, Dale W Griffin, Matthew T Moser, Mary K Beitz, Jorge Santo W DomingoAbstract:Wild birds have been shown to be significant sources of numerous types of pathogens that are relevant to humans and agriculture. The presence of large numbers of migratory birds in such a sensitive and important ecosystem as the Platte River in central Nebraska, USA, could potentially serve a significant source of bird-derived pathogens in the water/sediment and riverine environment. In 2009 and 2010, a study was completed to investigate the potential water-quality impacts of Sandhill Cranes and Snow Geese on the microbial water quality of the central Platte River during their spring migration period. Fecal material, river-bottom sediment, and water samples were collected from January through May of each year during the spring migration season of Sandhill Cranes in the Central Flyway of North America. Results indicate that several types of fecal indicator bacteria and from a range of viral, protozoan, and bacterial pathogens, Campylobacter jejuni were present in Sandhill Crane excreta, and at significantly higher frequency and densities in water and sediments when the Sandhill Cranes were present, particularly during evening roosts within the Platte River environment. Therefore, further investigation of the health significance of avian pathogens is warranted for the Platte River in Central Nebraska during migration of Sandhill Cranes and other waterfowl.
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development and evaluation of a quantitative pcr assay targeting Sandhill Crane grus canadensis fecal pollution
Applied and Environmental Microbiology, 2012Co-Authors: Hodon Ryu, Nicholas J. Ashbolt, Felipe Chavezramirez, Jason R Vogel, Michael Elk, Jorge Santo W DomingoAbstract:While the microbial water quality in the Platte River is seasonally impacted by excreta from migrating Cranes, there are no methods available to study Crane fecal contamination. Here we characterized microbial populations in Crane feces using phylogenetic analysis of 16S rRNA gene fecal clone libraries. Using these sequences, a novel Crane quantitative PCR (Crane1) assay was developed, and its applicability as a microbial source tracking (MST) assay was evaluated by determining its host specificity and detection ability in environmental waters. Bacteria from Crane excreta were dominated by bacilli and proteobacteria, with a notable paucity of sequences homologous to Bacteroidetes and Clostridia. The Crane1 marker targeted a dominant clade of unclassified Lactobacillales sequences closely related to Catellicoccus marimammalium. The host distribution of the Crane1 marker was relatively high, being positive for 69% (66/96) of the Crane excreta samples tested. The assay also showed high host specificity, with 95% of the nontarget fecal samples (i.e., n = 553; 20 different free-range hosts) being negative. Of the presumed Crane-impacted water samples (n = 16), 88% were positive for the Crane1 assay, whereas none of the water samples not impacted by Cranes were positive (n = 165). Bayesian statistical models of the Crane1 MST marker demonstrated high confidence in detecting true-positive signals and a low probability of false-negative signals from environmental water samples. Altogether, these data suggest that the newly developed marker could be used in environmental monitoring studies to study Crane fecal pollution dynamics.
Clemente-sánchez F. - One of the best experts on this subject based on the ideXlab platform.
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ESTADO DE CONSERVACIÓN DEL HABITAT PARA GRULLA GRIS (Grus canadensis) Y ANÁTIDOS ASOCIADOS EN HUMEDALES DE ZACATECAS
'Colegio de Postgraduados', 2018Co-Authors: Clemente-sánchez F.Abstract:A study was developed with the purpose of understanding the abundance and state of conservation of the Sandhill Crane (Grus canadensis) and migratory Anatidae in the priority wetlands that house them during the fall and winter seasons, in a sample of 24 sites with air and land monitoring. The results show that there was presence of Sandhill Crane in five reservoirs, with a total of 48 individuals, 17% of which were present over reed vegetation and 83% fed on the banks of the dams. During the months of November and December, 100% of the reservoirs sampled had water, with an average supply of >50% of their capacity, by the month of February completely dry saline lagoons were observed, and an average supply of 41% at the reservoirs with a moderate degree of erosion close to 5.62. The months November and December had abundant food for the Cranes, product of rainfed maize (Zea mays) harvesting. In February, the Cranes abandoned the Zacatecas wetlands and most of the lagoons were at 4 % of their supply. From November to February the abundance of ducks was 12,430 individuals and 4,560 geese. The habitat around the wetlands showed that local livestock production uses the vegetation at a level over 80 %, with the consequence of over-grazing. Based on the abundance of Sandhill Crane recorded, it is estimated that the semi-arid region of Zacatecas, México, is the southern limit of its migration, and that it may be in risk of disappearing in the area due to a low level of conservation and use of wetlands, while the associated Anatidae have greater availability of habitat during their migration and stay in the area.Se desarrolló un estudio basado en el monitoreo aéreo y terrestre en humedales de Zacatecas con el propósito de conocer la abundancia de grulla gris (Grus canadensis) y anátidos migratorios, así como el estado de conservación de los hábitats que los albergan durante el otoño e invierno. Los resultados mostraron que en cinco embalses se observó presencia de grulla gris con un total de 48 individuos, de los cuales 17% estuvieron presentes sobre vegetación de tule, y 83% alimentándose en la orilla de los embalses. Durante los meses de noviembre y diciembre, el 100% de los embalses muestreados mantuvieron agua, con un abasto promedio >50% de su capacidad total, y para el mes de febrero se observaron lagunas salinas completamente secas y un abasto promedio de los embalses de 41% con un grado moderado de erosión cercano a 5.62. Los meses de noviembre y diciembre tuvieron abundante alimento para las grullas, producto de la cosecha de maíz (Zea mays). Durante febrero, las grullas abandonaron los humedales de Zacatecas y la mayoría de las lagunas estuvieron a 4% de su capacidad de abasto. De noviembre a febrero, la abundancia de anátidos tales como, patos y ganso fue de 12,430 y 4,560 individuos respectivamente. El hábitat alrededor de los humedales mostró que la ganadería local hace uso de la vegetación en un nivel mayor a 80%, dando como consecuencia el sobrepastoreo. Con base en la abundancia registrada de la grulla gris, se estima que la región semiárida de Zacatecas, puede ser el límite sur de su migración, y que puede estar en riesgo de desaparecer en el área debido al bajo nivel de conservación y uso de los humedales, mientras que los anátidos asociados cuentan con mayor disponibilidad de hábitat para su migración y estancia en la región
