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Yoshikumi C - One of the best experts on this subject based on the ideXlab platform.
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Inhibition of HSP47 during the transition from solid to ascitic form of Sarcoma 180 in vivo.
In vivo (Athens Greece), 1995Co-Authors: Shirakami T, Tsuzuki T, Hiroko Iijima, Ishikawa Y, Kiyosuke Y, Morino M, Yoshimura M, Yoshikumi CAbstract:HSP47, a collagen-binding stress protein, has transformation-sensitivity; that is, the synthesis of HSP47 decreases after malignant transformation. We examined the expression of HSP47 in transplanted tumours by Western blotting. While HSP47 was not detected in ascites tumours including Ehrlich, P388 and Sarcoma 180, marked expression of HSP47 was observed in solid tumours such as B16, B16-BL6 and Sarcoma 180. The expression of HSP47 in Sarcoma 180 cells disappeared completely after these solid tumour cells were dispersed and inoculated intraperitoneally. These findings suggested an important role of HSP47 as a marker for tumour malignancy.
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Marked induction of HSP47, a collagen-binding stress protein, during solid tumor formation of ascitic Sarcoma 180 in vivo.
In vivo (Athens Greece), 1995Co-Authors: Morino M, Shirakami T, Tsuzuki T, Hiroko Iijima, Ishikawa Y, Kiyosuke Y, Yoshimura M, Yoshikumi CAbstract:HSP47, a heat shock-inducible glycoprotein of Mr = 47,000, is assumed to be a molecular chaperone specific for collagen. In addition to its stress inducibility, HSP47 has transformation-sensitivity; that is, the synthesis of HSP47 decreases after malignant transformation. We developed a rat monoclonal antibody against mouse HSP47, which we then used to examine the amounts of HSP47 in transplanted tumors. HSC70 (HSP73) was expressed constitutively in these ascites as well as solid tumors. While HSP47 was not expressed in ascitic form of Sarcoma 180, its expression was markedly induced during solid tumor formation of ascitic Sarcoma 180 cells after subcutaneous transplantation. The average survival period of ascites Sarcoma 180-bearing mice was 20.3 +/- 3.6 days (mean +/- SD), and was much shorter than that of solid Sarcoma 180-bearing mice. These findings suggest an important role of HSP47 in solid tumor formation as well as a possible marker for tumor malignancy.
Dalila De Brito Marques Ramos - One of the best experts on this subject based on the ideXlab platform.
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antinociceptive activity of schinus terebinthifolia leaf lectin stell in Sarcoma 180 bearing mice
Journal of Ethnopharmacology, 2020Co-Authors: Dalila De Brito Marques Ramos, Yasmym Araújo Silva, Maria Taís De Moura Fontes Araújo, Tarcisio Cicero De Lima Araujo, Angela Carolina Lima Amorim Dos Santos, Mariana Gama E Silva, Patricia Maria Guedes Paiva, Rosemairy Luciane Mendes, Thiago Henrique NapoleaoAbstract:Abstract Ethnopharmacology relevance Schinus terebinthifolia Raddi leaves have been used in folk medicine due to several properties, including antitumor and analgesic effects. The variable efficacy and adverse effects of analgesic drugs have motivated the search for novel antinociceptive agents. It has been reported that the S. terebinthifolia leaf lectin (SteLL) has antitumor activity against Sarcoma 180 in mice. Aim of the study This work aimed to evaluate whether SteLL would reduce cancer pain using an orthotopic tumor model. Materials and methods A Sarcoma 180 cell suspension was inoculated into the right hind paws of mice, and the treatments (150 mM NaCl, negative control; 10 mg/kg morphine, positive control; or SteLL at 1 and 2 mg/kg) were administered intraperitoneally 24 h after cell inoculation up to 14 days. Spontaneous nociception, mechanical hyperalgesia, and hot-plate tests were performed. Further, the volume and weight of the tumor-bearing paws were measured. Results SteLL (2 mg/kg) improved limb use during ambulation. The lectin (1 and 2 mg/kg) also inhibited mechanical hyperalgesia and increased the latency time during the hot-plate test. Naloxone was found to reverse this effect, indicating the involvement of opioid receptors. The tumor-bearing paws of mice treated with SteLL exhibited lower volume and weight. Conclusion SteLL reduced hyperalgesia due to Sarcoma 180 in the paws of mice, and this effect can be related to its antitumor action.
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Microgramma vacciniifolia frond lectin (MvFL) exhibits antitumor activity against Sarcoma 180 in mice
Phytomedicine Plus, 1Co-Authors: Leydianne Leite De Siqueira Patriota, Dalila De Brito Marques Ramos, Yasmym Araújo Silva, Angela Caroline Lima Amorim Dos Santos, Maria Taís De Moura Fontes Araújo, Jéssica De Santana Brito, Diego José Lira Torres, Alisson Macário De Oliveira, Diego César Nunes Da Silva, Virginia Maria Barros De LorenaAbstract:Abstract In this study, we evaluated the antitumor activity of a lectin from Microgramma vacciniifolia frond (MvFL) in Sarcoma 180-bearing mice. First, the in vitro cytotoxicity of MvFL on Sarcoma 180 cells was evaluated. Tumor cells were inoculated into Swiss female mice, and after 7 days, the animals were treated intraperitoneally with phosphate-buffered saline (PBS, negative control), methotrexate (MTX, positive control, 1.5 mg/kg) or MvFL (10 or 20 mg/kg) for 7 days. We analyzed tumor weight, angiogenesis, relative water and food consumption, body weight, and hematological, biochemical, and histological parameters. MvFL inhibited cell growth (IC50: 20.6 μg/mL) and induced apoptosis and necrosis. Treatment with MvFL led to an 89.2–96.8% reduction in tumor weight. Histopathological analysis of tumors of animals treated with MvFL revealed necrotic points, presence of leukocyte infiltrates, and a smaller number of pleomorphic cells than negative control. MvFL interferes with the angiogenesis around the Sarcoma 180 tumors. No changes in water and food consumption or weight gain were observed. There were no important changes in blood hematological and biochemical parameters in lectin-treated animals. The liver, kidneys, and spleen did not show signs of toxicity in all groups. In conclusion, MvFL has strong antitumor potential against Sarcoma 180 models, without toxic effects.
Maria Taís De Moura Fontes Araújo - One of the best experts on this subject based on the ideXlab platform.
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antinociceptive activity of schinus terebinthifolia leaf lectin stell in Sarcoma 180 bearing mice
Journal of Ethnopharmacology, 2020Co-Authors: Dalila De Brito Marques Ramos, Yasmym Araújo Silva, Maria Taís De Moura Fontes Araújo, Tarcisio Cicero De Lima Araujo, Angela Carolina Lima Amorim Dos Santos, Mariana Gama E Silva, Patricia Maria Guedes Paiva, Rosemairy Luciane Mendes, Thiago Henrique NapoleaoAbstract:Abstract Ethnopharmacology relevance Schinus terebinthifolia Raddi leaves have been used in folk medicine due to several properties, including antitumor and analgesic effects. The variable efficacy and adverse effects of analgesic drugs have motivated the search for novel antinociceptive agents. It has been reported that the S. terebinthifolia leaf lectin (SteLL) has antitumor activity against Sarcoma 180 in mice. Aim of the study This work aimed to evaluate whether SteLL would reduce cancer pain using an orthotopic tumor model. Materials and methods A Sarcoma 180 cell suspension was inoculated into the right hind paws of mice, and the treatments (150 mM NaCl, negative control; 10 mg/kg morphine, positive control; or SteLL at 1 and 2 mg/kg) were administered intraperitoneally 24 h after cell inoculation up to 14 days. Spontaneous nociception, mechanical hyperalgesia, and hot-plate tests were performed. Further, the volume and weight of the tumor-bearing paws were measured. Results SteLL (2 mg/kg) improved limb use during ambulation. The lectin (1 and 2 mg/kg) also inhibited mechanical hyperalgesia and increased the latency time during the hot-plate test. Naloxone was found to reverse this effect, indicating the involvement of opioid receptors. The tumor-bearing paws of mice treated with SteLL exhibited lower volume and weight. Conclusion SteLL reduced hyperalgesia due to Sarcoma 180 in the paws of mice, and this effect can be related to its antitumor action.
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Microgramma vacciniifolia frond lectin (MvFL) exhibits antitumor activity against Sarcoma 180 in mice
Phytomedicine Plus, 1Co-Authors: Leydianne Leite De Siqueira Patriota, Dalila De Brito Marques Ramos, Yasmym Araújo Silva, Angela Caroline Lima Amorim Dos Santos, Maria Taís De Moura Fontes Araújo, Jéssica De Santana Brito, Diego José Lira Torres, Alisson Macário De Oliveira, Diego César Nunes Da Silva, Virginia Maria Barros De LorenaAbstract:Abstract In this study, we evaluated the antitumor activity of a lectin from Microgramma vacciniifolia frond (MvFL) in Sarcoma 180-bearing mice. First, the in vitro cytotoxicity of MvFL on Sarcoma 180 cells was evaluated. Tumor cells were inoculated into Swiss female mice, and after 7 days, the animals were treated intraperitoneally with phosphate-buffered saline (PBS, negative control), methotrexate (MTX, positive control, 1.5 mg/kg) or MvFL (10 or 20 mg/kg) for 7 days. We analyzed tumor weight, angiogenesis, relative water and food consumption, body weight, and hematological, biochemical, and histological parameters. MvFL inhibited cell growth (IC50: 20.6 μg/mL) and induced apoptosis and necrosis. Treatment with MvFL led to an 89.2–96.8% reduction in tumor weight. Histopathological analysis of tumors of animals treated with MvFL revealed necrotic points, presence of leukocyte infiltrates, and a smaller number of pleomorphic cells than negative control. MvFL interferes with the angiogenesis around the Sarcoma 180 tumors. No changes in water and food consumption or weight gain were observed. There were no important changes in blood hematological and biochemical parameters in lectin-treated animals. The liver, kidneys, and spleen did not show signs of toxicity in all groups. In conclusion, MvFL has strong antitumor potential against Sarcoma 180 models, without toxic effects.
Yasmym Araújo Silva - One of the best experts on this subject based on the ideXlab platform.
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antinociceptive activity of schinus terebinthifolia leaf lectin stell in Sarcoma 180 bearing mice
Journal of Ethnopharmacology, 2020Co-Authors: Dalila De Brito Marques Ramos, Yasmym Araújo Silva, Maria Taís De Moura Fontes Araújo, Tarcisio Cicero De Lima Araujo, Angela Carolina Lima Amorim Dos Santos, Mariana Gama E Silva, Patricia Maria Guedes Paiva, Rosemairy Luciane Mendes, Thiago Henrique NapoleaoAbstract:Abstract Ethnopharmacology relevance Schinus terebinthifolia Raddi leaves have been used in folk medicine due to several properties, including antitumor and analgesic effects. The variable efficacy and adverse effects of analgesic drugs have motivated the search for novel antinociceptive agents. It has been reported that the S. terebinthifolia leaf lectin (SteLL) has antitumor activity against Sarcoma 180 in mice. Aim of the study This work aimed to evaluate whether SteLL would reduce cancer pain using an orthotopic tumor model. Materials and methods A Sarcoma 180 cell suspension was inoculated into the right hind paws of mice, and the treatments (150 mM NaCl, negative control; 10 mg/kg morphine, positive control; or SteLL at 1 and 2 mg/kg) were administered intraperitoneally 24 h after cell inoculation up to 14 days. Spontaneous nociception, mechanical hyperalgesia, and hot-plate tests were performed. Further, the volume and weight of the tumor-bearing paws were measured. Results SteLL (2 mg/kg) improved limb use during ambulation. The lectin (1 and 2 mg/kg) also inhibited mechanical hyperalgesia and increased the latency time during the hot-plate test. Naloxone was found to reverse this effect, indicating the involvement of opioid receptors. The tumor-bearing paws of mice treated with SteLL exhibited lower volume and weight. Conclusion SteLL reduced hyperalgesia due to Sarcoma 180 in the paws of mice, and this effect can be related to its antitumor action.
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Microgramma vacciniifolia frond lectin (MvFL) exhibits antitumor activity against Sarcoma 180 in mice
Phytomedicine Plus, 1Co-Authors: Leydianne Leite De Siqueira Patriota, Dalila De Brito Marques Ramos, Yasmym Araújo Silva, Angela Caroline Lima Amorim Dos Santos, Maria Taís De Moura Fontes Araújo, Jéssica De Santana Brito, Diego José Lira Torres, Alisson Macário De Oliveira, Diego César Nunes Da Silva, Virginia Maria Barros De LorenaAbstract:Abstract In this study, we evaluated the antitumor activity of a lectin from Microgramma vacciniifolia frond (MvFL) in Sarcoma 180-bearing mice. First, the in vitro cytotoxicity of MvFL on Sarcoma 180 cells was evaluated. Tumor cells were inoculated into Swiss female mice, and after 7 days, the animals were treated intraperitoneally with phosphate-buffered saline (PBS, negative control), methotrexate (MTX, positive control, 1.5 mg/kg) or MvFL (10 or 20 mg/kg) for 7 days. We analyzed tumor weight, angiogenesis, relative water and food consumption, body weight, and hematological, biochemical, and histological parameters. MvFL inhibited cell growth (IC50: 20.6 μg/mL) and induced apoptosis and necrosis. Treatment with MvFL led to an 89.2–96.8% reduction in tumor weight. Histopathological analysis of tumors of animals treated with MvFL revealed necrotic points, presence of leukocyte infiltrates, and a smaller number of pleomorphic cells than negative control. MvFL interferes with the angiogenesis around the Sarcoma 180 tumors. No changes in water and food consumption or weight gain were observed. There were no important changes in blood hematological and biochemical parameters in lectin-treated animals. The liver, kidneys, and spleen did not show signs of toxicity in all groups. In conclusion, MvFL has strong antitumor potential against Sarcoma 180 models, without toxic effects.
Taesoo Lee - One of the best experts on this subject based on the ideXlab platform.
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antitumor and immuno potentiating activity against mouse Sarcoma 180 by crude polysaccharides extracted from fruiting body of tricholoma matsutake
Journal of Life Science, 2008Co-Authors: Hyun Hur, Yonil Choi, Taesoo LeeAbstract:Tricholoma matsutake, one of edible and medicinal mushroom belonging to Tricholomaceae of Agaricales, has been known to contain some curing effect on gastric cancer and ulcer, and inhibitory effect on Sarcoma 180 and Ehrlich Sarcoma. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests, crude polysaccharides were not cytotoxic against cancer cell lines such as Sarcoma 180, HepG2, HT29 and NIH3T3 at the concentration of 2.0 mg/ml. Intraperitoneal injection with crude polysaccharides showed life prolongation effect of 23.437.2% in mice previously inoculated with Sarcoma 180. Fr. MeOH and Fr. HW exhibited the immuno-potentiating activity of B lymphocyte by increasing the alkaline phosphatase activity by 2.211.9 folds compared with control at the concentration of 0.20.5 mg/ml. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 6.0 and 1.5 folds at the concentration of 50 mg/kg, respectively. Therefore, it is concluded that crude polysaccharides extracted from fruiting body of Tricholoma matsutake showed antitumor and immuno-potentiating activity against Sarcoma 180 of mouse.
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antitumor and immuno modulatory effect of crude polysaccharides from fruiting body of tremella aurantialba against mouse Sarcoma 180
The Korean Journal of Mycology, 2008Co-Authors: Geon-woo Lee, Min-woong Lee, Hye Young Kim, M J Shim, Uyoun Lee, Hyun Hur, Taesoo LeeAbstract:Tremella aurantialba, one of edible and medicinal mushrooms belonging to Tremellaceae of Basidiomycota, has been known to have outstanding curing effects on coughing, tracheitis and hypertension of humans and antitumor activity on the Sarcoma 180 and Erhrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests showed that all crude polysaccharides extracted from the fruiting body were not cytotoxic against cancer cell lines such as RAW 309 CR.1 and Sarcoma 180 at the concentration of . Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of in mice inoculated with Sarcoma 180. Fr. MeOH improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by 1.16 folds at the concentration of . In case of Fr. HW, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 1.42 and 2.87 folds, respectively.
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antitumor and immuno modulatory effect against mouse Sarcoma 180 of crude polysaccharides extracted from fruiting body of armillaria tabescens
The Korean Journal of Mycology, 2007Co-Authors: Geon-woo Lee, Hye Young Kim, Uyoun Lee, Taesoo LeeAbstract:Armillaria tabescens, one of edible and medicinal mushrooms belonging to Agaricales of Basidiomycota, has been known to have outstanding curative effects on chronic hepatitis and cholecystitis and inhibitory effects on the Sarcoma 180 and Erhrlich carcinoma of mice. Neutral saline soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr, NaCl, Fr. HW and Fr, MeOH, respectively) were extracted from fruiting body of the mushroom. In vitro cytotoxicity tests showed that crude polysaccharides were not cytotoxic against cancer cell lines such as NIH3T3 and Sarcoma 180 at the concentration of . Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of in mice inoculated with Sarcoma 180, respectively. Fr. NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by folds, respectively. In case of Fr, NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 9 and 1.9 folds, respectively.
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inhibitive effect of mouse Sarcoma 180 by crude polysaccharide extracted from fruiting body of aramillaria mellea
The Korean Journal of Mycology, 2006Co-Authors: Sangbeom Kim, Min-woong Lee, Gunwoo Lee, Hye Young Kim, M J Shim, Hyunsu Rho, Hyunsook Lee, Uyoun Lee, Taesoo LeeAbstract:Armillaria mellea, one of edible and medicinal mushroom belonging to Tricholomataceae of Basid-iomycota, has been known to have outstanding inhibitive effects on the Sarcoma 180 and Erhrlich carcinoma of mice. Neutral salt soluble (0.9% NaCl), hot water soluble and methanol soluble substances (hereinafter referred to Fr. NaCl, Fr, HW and Fr. MeOH, respectively) were extracted from the mushroom. In vitro cytotoxicity tests, crude polysaccharide were not cytotoxic against cancer cell lines such as NIH3T3 and Sarcoma 180 at the concentration of . Intraperitoneal injection with crude polysaccharides exhibited life prolongation effect of in mice inoculated with Sarcoma 180, respectively. Fr, NaCl improved the immunopotentiation activity of B lymphocyte by increasing the alkaline phosphatase activity by folds, respectively. In case of Fr. NaCl, the numbers of peritoneal exudate cells and circulating leukocytes were increased by 10 and 2 folds, respectively.
