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You-jin Jeon - One of the best experts on this subject based on the ideXlab platform.

  • Sargassum horneri turner c agardh containing polyphenols attenuates particulate matter induced inflammatory response by blocking tlr mediated myd88 dependent mapk signaling pathway in mle 12 cells
    Journal of Ethnopharmacology, 2021
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, Ju Hee Lee, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, YOUNG HEUN JEE
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS In this study, we evaluated the impact of PM (majority <2.5 μm in diameter) on deep bronchial penetration ability upon inhalation and a therapeutic approach to mitigate its harmful effects using an ethanol extract of Sargassum horneri, an edible brown algae, containing polyphenols on a type II alveolar epithelial cell line, MLE-12. RESULTS PM triggered mRNA expression of toll-like receptors (TLRs) TLR2/4/7, and those TLRs were significantly attenuated by Sargassum horneri extract (SHE). SHE further attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) p38, extracellular signal-regulated kinase 1/2 (Erk1/2), and c-Jun NH (2)-terminal kinase (JNK), which were also activated in PM-exposed cells. Altogether, SHE subdued the PM-induced mRNA expression of pro-inflammatory cytokines (interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6) and lung epithelial cell derived-chemokines (IL-8, monocyte chemoattractant protein-1 (MCP-1), and chemokine (C-C motif) ligand 5 (CCL5)). SHE also suppressed the mRNA expression of PM-induced pro-allergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin (IL)-33. Furthermore, we showed that SHE suppressed the MAPK-dependent signaling pathway by attenuating receptor-associated factor (TRAF) 6 activation of proteins MyD88 and TNF. CONCLUSION Taking all the data together, we suggest that the anti-inflammatory potential of SHE on PM-exposed MLE-12 cells is mediated by the inhibition of PM-triggered downstream signaling along the TLR2/4/7-MyD88-TRAF6 axis of MAPK signaling.

  • Sargassum horneri (Turner) C. Agardh containing polyphenols attenuates particulate matter-induced inflammatory response by blocking TLR-mediated MYD88-dependent MAPK signaling pathway in MLE-12 cells.
    Journal of ethnopharmacology, 2020
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, Ju Hee Lee, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, YOUNG HEUN JEE
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS In this study, we evaluated the impact of PM (majority

  • Antioxidant efficacy of (−)-loliolide isolated from Sargassum horneri against AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Lei Wang, Ilekuttige Priyan Shanura Fernando, Jun-geon Je, Seok-chun Ko, Min Cheol Kang, You-jin Jeon
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC_50: 0.043 ± 0.005 mg mL^−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • Antioxidant efficacy of (−)-loliolide isolated from Sargassum horneri against AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Hyun-soo Kim, You-jin Jeon, Lei Wang, Ilekuttige Priyan Shanura Fernando, Min Cheol Kang, Jeong Min Lee, Mi-jin Yim, Dae Sung Lee
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 ± 0.005 mg mL−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • antioxidant efficacy of loliolide isolated from Sargassum horneri against aaph induced oxidative damage in vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Hyun-soo Kim, You-jin Jeon, Lei Wang, Ilekuttige Priyan Shanura Fernando, Min Cheol Kang, Jeong Min Lee, Mi-jin Yim, Dae Sung Lee
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 ± 0.005 mg mL−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

YOUNG HEUN JEE - One of the best experts on this subject based on the ideXlab platform.

  • Sargassum horneri turner c agardh containing polyphenols attenuates particulate matter induced inflammatory response by blocking tlr mediated myd88 dependent mapk signaling pathway in mle 12 cells
    Journal of Ethnopharmacology, 2021
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, Ju Hee Lee, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, YOUNG HEUN JEE
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS In this study, we evaluated the impact of PM (majority <2.5 μm in diameter) on deep bronchial penetration ability upon inhalation and a therapeutic approach to mitigate its harmful effects using an ethanol extract of Sargassum horneri, an edible brown algae, containing polyphenols on a type II alveolar epithelial cell line, MLE-12. RESULTS PM triggered mRNA expression of toll-like receptors (TLRs) TLR2/4/7, and those TLRs were significantly attenuated by Sargassum horneri extract (SHE). SHE further attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) p38, extracellular signal-regulated kinase 1/2 (Erk1/2), and c-Jun NH (2)-terminal kinase (JNK), which were also activated in PM-exposed cells. Altogether, SHE subdued the PM-induced mRNA expression of pro-inflammatory cytokines (interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6) and lung epithelial cell derived-chemokines (IL-8, monocyte chemoattractant protein-1 (MCP-1), and chemokine (C-C motif) ligand 5 (CCL5)). SHE also suppressed the mRNA expression of PM-induced pro-allergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin (IL)-33. Furthermore, we showed that SHE suppressed the MAPK-dependent signaling pathway by attenuating receptor-associated factor (TRAF) 6 activation of proteins MyD88 and TNF. CONCLUSION Taking all the data together, we suggest that the anti-inflammatory potential of SHE on PM-exposed MLE-12 cells is mediated by the inhibition of PM-triggered downstream signaling along the TLR2/4/7-MyD88-TRAF6 axis of MAPK signaling.

  • Sargassum horneri (Turner) C. Agardh containing polyphenols attenuates particulate matter-induced inflammatory response by blocking TLR-mediated MYD88-dependent MAPK signaling pathway in MLE-12 cells.
    Journal of ethnopharmacology, 2020
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, Ju Hee Lee, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, YOUNG HEUN JEE
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS In this study, we evaluated the impact of PM (majority

  • Sargassum horneri extract containing mojabanchromanol attenuates the particulate matter exacerbated allergic asthma through reduction of Th2 and Th17 response in mice.
    Environmental pollution (Barking Essex : 1987), 2020
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, Suyama Prasansali Mihindukulasooriya, Areum Kim, YOUNG HEUN JEE
    Abstract:

    Abstract Airborne particulate matter (PM) has become a serious health issue causing pulmonary diseases such as asthma. Due to the side effects and non-specificity of conventional drugs, there is a need to develop natural-product-based alternative treatments. Sargassum horneri is a brown alga shown to have anti-oxidant, anti-inflammatory, and anti-allergic effects. Thus, we sought to determine whether ethanol extract of Sargassum horneri (SHE) mitigates the effect of PM exposure on asthma development. To establish a mouse model of asthma, BALB/c mice were sensitized with ovalbumin (OVA, 10 μg) and challenged with PM (5 mg/m3) for 7 days consecutively. SHE (200, 400 mg/kg), Prednisone (5 mg/kg), or PBS was daily administrated orally before PM exposure. SHE mitigated PM exacerbated dendritic cell activation. More importantly, SHE restrained Th2 polarization by attenuating transcription factors GATA3 and STAT5, which further mitigated the expression of Th2 cytokines interleukin (IL)-4, IL-5, and IL-13 in the lung homogenates of PM-exacerbated asthmatic mice. SHE further attenuated PM-exacerbated eosinophil infiltration in the lung, trachea, and BALF. In addition, SHE markedly mitigated the activation of mast cells and the IgE level in serum. Concomitantly, SHE further restrained the Th17 cell response in PM-exposed allergic mice through attenuating expression of transcription factors RORγT, STAT3 and expression of relevant effector cytokines IL-17a. This resulted in mitigated neutrophil infiltration in the lung. Taken together, SHE significantly suppressed PM-exacerbated hypersecretion of mucus in asthmatic mice. These results suggest that SHE has therapeutic potential for treating PM-exacerbated allergic asthma through concomitantly inhibiting Th2/Th17 responses.

  • Antioxidant potential of Sargassum horneri extract against urban particulate matter-induced oxidation
    Food Science and Biotechnology, 2020
    Co-Authors: Ju Hee Lee, YOUNG HEUN JEE, You-jin Jeon, Hyo-jin Kim, Hyun Jung Kim
    Abstract:

    Particulate matter is a major contribution of air pollution and detrimental to human health. The in vitro antioxidant activities of a brown seaweed, Sargassum horneri ethanol extract (SHE) against particulate matter-induced oxidative stress were investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging activity, hydrogen peroxide (H_2O_2) scavenging activity, superoxide anion (O _2^ · − ) inhibition, hydroxyl radical (^ · OH) scavenging activity, reducing power, and the metal ion-chelating effect. All in vitro antioxidant activities were increased as the concentration of SHE increased (0–1000 μg/mL). When treated with particulate matter at 0–1000 μg/mL, the DPPH free radical, and H_2O_2 scavenging activities, reducing power, and metal ion-chelating abilities of SHE were significantly decreased ( p 

  • antioxidant potential of Sargassum horneri extract against urban particulate matter induced oxidation
    Food Science and Biotechnology, 2020
    Co-Authors: Ju Hee Lee, YOUNG HEUN JEE, You-jin Jeon, Hyo-jin Kim, Hyun Jung Kim
    Abstract:

    Particulate matter is a major contribution of air pollution and detrimental to human health. The in vitro antioxidant activities of a brown seaweed, Sargassum horneri ethanol extract (SHE) against particulate matter-induced oxidative stress were investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity, superoxide anion (O2− ) inhibition, hydroxyl radical (·OH) scavenging activity, reducing power, and the metal ion-chelating effect. All in vitro antioxidant activities were increased as the concentration of SHE increased (0–1000 μg/mL). When treated with particulate matter at 0–1000 μg/mL, the DPPH free radical, and H2O2 scavenging activities, reducing power, and metal ion-chelating abilities of SHE were significantly decreased (p < 0.05). These results indicate that Sargassum horneri, which is a rich source of bioactive compounds, can be used as a natural source of antioxidants in the food industries.

Hyun-soo Kim - One of the best experts on this subject based on the ideXlab platform.

  • Antioxidant efficacy of (−)-loliolide isolated from Sargassum horneri against AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Hyun-soo Kim, You-jin Jeon, Lei Wang, Ilekuttige Priyan Shanura Fernando, Min Cheol Kang, Jeong Min Lee, Mi-jin Yim, Dae Sung Lee
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 ± 0.005 mg mL−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • antioxidant efficacy of loliolide isolated from Sargassum horneri against aaph induced oxidative damage in vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Hyun-soo Kim, You-jin Jeon, Lei Wang, Ilekuttige Priyan Shanura Fernando, Min Cheol Kang, Jeong Min Lee, Mi-jin Yim, Dae Sung Lee
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 ± 0.005 mg mL−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • (-)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes.
    Antioxidants (Basel Switzerland), 2020
    Co-Authors: Mawalle Kankanamge Hasitha Madhawa Dias, You-jin Jeon, Hyun-soo Kim, Eui Jeong Han, Ilekuttige Priyan Shanura Fernando, Dissanayaka Mudiyanselage Dinesh Madusanka, Min Ju Kim, Ginnae Ahn
    Abstract:

    The emergence of fine dust (FD) among air pollutants has taken a toll during the past few decades, and it has provided both controversy and a platform for open conversation amongst world powers for finding sustainable solutions and effective treatments for health issues. The present study emphasizes the protective effects of (–)-loliolide (HTT) isolated from Sargassum horneri against FD-induced oxidative stress in human HaCaT keratinocytes. The purification of (–)-loliolide was carried out by centrifugal partition chromatography. HTT did not show any cytotoxicity, and it further illustrated the potential to increase cell viability by reducing the reactive oxygen species (ROS) production in FD-stimulated keratinocytes. Furthermore, HTT suppressed FD-stimulated DNA damage and the formation of apoptotic bodies, and it reduced the population of cells in the sub-G1 apoptosis phase. FD-induced apoptosis was advancing through the mitochondria-mediated apoptosis pathway. The cytoprotective effects of the HTT against FD-stimulated oxidative damage is mediated through squaring the nuclear factor E2-related factor 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) pathway, dose-dependently increasing HO-1 and NAD(P)H dehydrogenase (quinone) 1 (NQO1) levels in the cytosol while concomitantly improving the nuclear translocation of Nrf2. Future studies could implement the protective functionality of HTT in producing pharmaceuticals that utilize natural products and benefit the diseased.

  • Anti-allergy effect of mojabanchromanol isolated from Sargassum horneri in bone marrow-derived cultured mast cells
    Algal Research, 2020
    Co-Authors: Hyun-soo Kim, YOUNG HEUN JEE, Thilina U. Jayawardena, Eui Jeong Han, Ilekuttige Priyan Shanura Fernando, Kalu Kapuge Asanka Sanjeewa, Hak-ju Kim, Seo-hee Kang, Jae-hyuk Jang, Jun-pil Jang
    Abstract:

    Abstract This study was conducted to isolate and identify an active compound possessing anti-allergic property from the Jeju Island inhabiting Sargassum horneri (S. horneri). The ethanol extract of S. horneri (SHE) exhibited an anti-allergic effect via the reduction of β-hexosaminidase release in immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated bone marrow-derived cultured mast cells (BMCMCs). SHE was subjected to liquid phase fractionation obtaining the hexane fraction, and this was further purified via ODS column chromatography and prep-C18 HPLC. The isolated compound was identified as mojabanchromanol (MC) by 1H, 13C NMR, and LCMS analysis. Isolated MC showed a higher inhibitory effect on the β-hexosaminidase release than those of SHE without cytotoxicity. Moreover, MC inhibited the mRNA expression levels of allergic cytokines in BMCMCs. This is the first report to inform on the anti-allergic property of MC isolated from S. horneri. In conclusion, SHE, as well as MC, is applicable in the development of functional health food, cosmetics, and pharmaceutical products exhibiting anti-allergic effects.

  • ethanol extract separated from Sargassum horneri turner abate lps induced inflammation in raw 264 7 macrophages
    Fisheries and Aquatic Sciences, 2019
    Co-Authors: K. Asanka K. Sanjeewa, YOUNG HEUN JEE, Thilina U. Jayawardena, Hyun-soo Kim, Hak-ju Kim, Seo-young Kim, Ginnae Ahn, You-jin Jeon
    Abstract:

    This study is aimed at identifying the anti-inflammatory properties of 70% ethanol extract produced from an edible brown seaweed Sargassum horneri (SJB-SHE) with industrial-scale production by Seojin Biotech Co. Ltd. S. horneri is a rich source of nutrient and abundantly growing along the shores of Jeju, South Korea. Here, we investigated the effect of SJB-SHE on LPS-activated RAW 264.7 macrophages. The cytotoxicity and NO production of SJB-SHE were evaluated using MTT and Griess assays, respectively. Additionally, protein expression and gene expression levels were quantified using ELISA, Western blots, and RT-qPCR. Our results indicated that pre-treatment of RAW 264.7 macrophages with SJB-SHE significantly inhibited LPS-induced NO and PGE2 production. SJB-SHE downregulated the proteins and genes expression of LPS-induced iNOS and COX2. Additionally, SJB-SHE downregulated LPS-induced production of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin (IL)-6, and IL-1β). Furthermore, SJB-SHE inhibited nuclear factor kappa-B (NF-κB) activation and translocation to the nucleus. SJB-SHE also suppressed the phosphorylation of mitogen-activated protein kinases (ERK1/2 and JNK). Collectively, our results demonstrated that SJB-SHE has a potential anti-inflammatory property to use as a functional food ingredient in the future.

Ilekuttige Priyan Shanura Fernando - One of the best experts on this subject based on the ideXlab platform.

  • Antioxidant efficacy of (−)-loliolide isolated from Sargassum horneri against AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Lei Wang, Ilekuttige Priyan Shanura Fernando, Jun-geon Je, Seok-chun Ko, Min Cheol Kang, You-jin Jeon
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC_50: 0.043 ± 0.005 mg mL^−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • Antioxidant efficacy of (−)-loliolide isolated from Sargassum horneri against AAPH-induced oxidative damage in Vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Hyun-soo Kim, You-jin Jeon, Lei Wang, Ilekuttige Priyan Shanura Fernando, Min Cheol Kang, Jeong Min Lee, Mi-jin Yim, Dae Sung Lee
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 ± 0.005 mg mL−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • antioxidant efficacy of loliolide isolated from Sargassum horneri against aaph induced oxidative damage in vero cells and zebrafish models in vivo
    Journal of Applied Phycology, 2020
    Co-Authors: Hyun-soo Kim, You-jin Jeon, Lei Wang, Ilekuttige Priyan Shanura Fernando, Min Cheol Kang, Jeong Min Lee, Mi-jin Yim, Dae Sung Lee
    Abstract:

    Seaweed-derived compounds are known to possess various biological activities. The present study evaluated the antioxidant potential of (−)-loliolide, a monoterpenoid isolated from Sargassum horneri in Vero cells and zebrafish models. (−)-Loliolide was purified from the chloroform fraction of an S. horneri methanol extract using high-performance centrifugal partition chromatography (HPCPC). HPCPC was found to be an effective method to isolate (−)-loliolide, yielding a high purity product. Electron spin resonance evaluation demonstrated that (−)-loliolide had significant alkyl radical scavenging activity (IC50: 0.043 ± 0.005 mg mL−1). In addition, our study showed that (−)-loliolide demonstrated dose-dependent protective effects for Vero cell viability against 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced intracellular reactive oxygen species (ROS). Moreover, (−)-loliolide reduced lipid peroxidation in AAPH-induced zebrafish embryos, while ameliorating the survival rate and cell death in a dose-dependent manner. These results reveal the antioxidant potential of (−)-loliolide. Future studies highlighting the potential applications of S. horneri in manufacturing functional food ingredients are required.

  • (-)-Loliolide Isolated from Sargassum horneri Protects against Fine Dust-Induced Oxidative Stress in Human Keratinocytes.
    Antioxidants (Basel Switzerland), 2020
    Co-Authors: Mawalle Kankanamge Hasitha Madhawa Dias, You-jin Jeon, Hyun-soo Kim, Eui Jeong Han, Ilekuttige Priyan Shanura Fernando, Dissanayaka Mudiyanselage Dinesh Madusanka, Min Ju Kim, Ginnae Ahn
    Abstract:

    The emergence of fine dust (FD) among air pollutants has taken a toll during the past few decades, and it has provided both controversy and a platform for open conversation amongst world powers for finding sustainable solutions and effective treatments for health issues. The present study emphasizes the protective effects of (–)-loliolide (HTT) isolated from Sargassum horneri against FD-induced oxidative stress in human HaCaT keratinocytes. The purification of (–)-loliolide was carried out by centrifugal partition chromatography. HTT did not show any cytotoxicity, and it further illustrated the potential to increase cell viability by reducing the reactive oxygen species (ROS) production in FD-stimulated keratinocytes. Furthermore, HTT suppressed FD-stimulated DNA damage and the formation of apoptotic bodies, and it reduced the population of cells in the sub-G1 apoptosis phase. FD-induced apoptosis was advancing through the mitochondria-mediated apoptosis pathway. The cytoprotective effects of the HTT against FD-stimulated oxidative damage is mediated through squaring the nuclear factor E2-related factor 2 (Nrf2)-mediated heme oxygenase-1 (HO-1) pathway, dose-dependently increasing HO-1 and NAD(P)H dehydrogenase (quinone) 1 (NQO1) levels in the cytosol while concomitantly improving the nuclear translocation of Nrf2. Future studies could implement the protective functionality of HTT in producing pharmaceuticals that utilize natural products and benefit the diseased.

  • Anti-allergy effect of mojabanchromanol isolated from Sargassum horneri in bone marrow-derived cultured mast cells
    Algal Research, 2020
    Co-Authors: Hyun-soo Kim, YOUNG HEUN JEE, Thilina U. Jayawardena, Eui Jeong Han, Ilekuttige Priyan Shanura Fernando, Kalu Kapuge Asanka Sanjeewa, Hak-ju Kim, Seo-hee Kang, Jae-hyuk Jang, Jun-pil Jang
    Abstract:

    Abstract This study was conducted to isolate and identify an active compound possessing anti-allergic property from the Jeju Island inhabiting Sargassum horneri (S. horneri). The ethanol extract of S. horneri (SHE) exhibited an anti-allergic effect via the reduction of β-hexosaminidase release in immunoglobulin E (IgE)/bovine serum albumin (BSA)-stimulated bone marrow-derived cultured mast cells (BMCMCs). SHE was subjected to liquid phase fractionation obtaining the hexane fraction, and this was further purified via ODS column chromatography and prep-C18 HPLC. The isolated compound was identified as mojabanchromanol (MC) by 1H, 13C NMR, and LCMS analysis. Isolated MC showed a higher inhibitory effect on the β-hexosaminidase release than those of SHE without cytotoxicity. Moreover, MC inhibited the mRNA expression levels of allergic cytokines in BMCMCs. This is the first report to inform on the anti-allergic property of MC isolated from S. horneri. In conclusion, SHE, as well as MC, is applicable in the development of functional health food, cosmetics, and pharmaceutical products exhibiting anti-allergic effects.

Hyun Jung Kim - One of the best experts on this subject based on the ideXlab platform.

  • Sargassum horneri turner c agardh containing polyphenols attenuates particulate matter induced inflammatory response by blocking tlr mediated myd88 dependent mapk signaling pathway in mle 12 cells
    Journal of Ethnopharmacology, 2021
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, Ju Hee Lee, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, YOUNG HEUN JEE
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS In this study, we evaluated the impact of PM (majority <2.5 μm in diameter) on deep bronchial penetration ability upon inhalation and a therapeutic approach to mitigate its harmful effects using an ethanol extract of Sargassum horneri, an edible brown algae, containing polyphenols on a type II alveolar epithelial cell line, MLE-12. RESULTS PM triggered mRNA expression of toll-like receptors (TLRs) TLR2/4/7, and those TLRs were significantly attenuated by Sargassum horneri extract (SHE). SHE further attenuated the phosphorylation of mitogen-activated protein kinase (MAPK) p38, extracellular signal-regulated kinase 1/2 (Erk1/2), and c-Jun NH (2)-terminal kinase (JNK), which were also activated in PM-exposed cells. Altogether, SHE subdued the PM-induced mRNA expression of pro-inflammatory cytokines (interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-6) and lung epithelial cell derived-chemokines (IL-8, monocyte chemoattractant protein-1 (MCP-1), and chemokine (C-C motif) ligand 5 (CCL5)). SHE also suppressed the mRNA expression of PM-induced pro-allergic cytokines thymic stromal lymphopoietin (TSLP) and interleukin (IL)-33. Furthermore, we showed that SHE suppressed the MAPK-dependent signaling pathway by attenuating receptor-associated factor (TRAF) 6 activation of proteins MyD88 and TNF. CONCLUSION Taking all the data together, we suggest that the anti-inflammatory potential of SHE on PM-exposed MLE-12 cells is mediated by the inhibition of PM-triggered downstream signaling along the TLR2/4/7-MyD88-TRAF6 axis of MAPK signaling.

  • Sargassum horneri (Turner) C. Agardh containing polyphenols attenuates particulate matter-induced inflammatory response by blocking TLR-mediated MYD88-dependent MAPK signaling pathway in MLE-12 cells.
    Journal of ethnopharmacology, 2020
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, Ju Hee Lee, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, YOUNG HEUN JEE
    Abstract:

    ETHNOPHARMACOLOGICAL RELEVANCE Sargassum horneri (Turner) C. Agardh (S. horneri), an edible brown marine algae, is known to have immunomodulatory effects and has been used in oriental medicine to treat inflammatory diseases. It is well known that ambient particulate matter (PM) is closely related to increased respiratory diseases inducing lung inflammation. AIM Considering the use of Sargassum horneri in traditional medicine to treat inflammatory diseases, we hypothesized and investigated the use of Sargassum horneri containing polyphenols against PM-induced inflammatory responses. MATERIALS AND METHODS In this study, we evaluated the impact of PM (majority

  • Sargassum horneri extract containing mojabanchromanol attenuates the particulate matter exacerbated allergic asthma through reduction of Th2 and Th17 response in mice.
    Environmental pollution (Barking Essex : 1987), 2020
    Co-Authors: Kalahe Hewage Iresha Nadeeka Madushani Herath, You-jin Jeon, Hyun Jung Kim, Hyo-jin Kim, Suyama Prasansali Mihindukulasooriya, Areum Kim, YOUNG HEUN JEE
    Abstract:

    Abstract Airborne particulate matter (PM) has become a serious health issue causing pulmonary diseases such as asthma. Due to the side effects and non-specificity of conventional drugs, there is a need to develop natural-product-based alternative treatments. Sargassum horneri is a brown alga shown to have anti-oxidant, anti-inflammatory, and anti-allergic effects. Thus, we sought to determine whether ethanol extract of Sargassum horneri (SHE) mitigates the effect of PM exposure on asthma development. To establish a mouse model of asthma, BALB/c mice were sensitized with ovalbumin (OVA, 10 μg) and challenged with PM (5 mg/m3) for 7 days consecutively. SHE (200, 400 mg/kg), Prednisone (5 mg/kg), or PBS was daily administrated orally before PM exposure. SHE mitigated PM exacerbated dendritic cell activation. More importantly, SHE restrained Th2 polarization by attenuating transcription factors GATA3 and STAT5, which further mitigated the expression of Th2 cytokines interleukin (IL)-4, IL-5, and IL-13 in the lung homogenates of PM-exacerbated asthmatic mice. SHE further attenuated PM-exacerbated eosinophil infiltration in the lung, trachea, and BALF. In addition, SHE markedly mitigated the activation of mast cells and the IgE level in serum. Concomitantly, SHE further restrained the Th17 cell response in PM-exposed allergic mice through attenuating expression of transcription factors RORγT, STAT3 and expression of relevant effector cytokines IL-17a. This resulted in mitigated neutrophil infiltration in the lung. Taken together, SHE significantly suppressed PM-exacerbated hypersecretion of mucus in asthmatic mice. These results suggest that SHE has therapeutic potential for treating PM-exacerbated allergic asthma through concomitantly inhibiting Th2/Th17 responses.

  • Antioxidant potential of Sargassum horneri extract against urban particulate matter-induced oxidation
    Food Science and Biotechnology, 2020
    Co-Authors: Ju Hee Lee, YOUNG HEUN JEE, You-jin Jeon, Hyo-jin Kim, Hyun Jung Kim
    Abstract:

    Particulate matter is a major contribution of air pollution and detrimental to human health. The in vitro antioxidant activities of a brown seaweed, Sargassum horneri ethanol extract (SHE) against particulate matter-induced oxidative stress were investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging activity, hydrogen peroxide (H_2O_2) scavenging activity, superoxide anion (O _2^ · − ) inhibition, hydroxyl radical (^ · OH) scavenging activity, reducing power, and the metal ion-chelating effect. All in vitro antioxidant activities were increased as the concentration of SHE increased (0–1000 μg/mL). When treated with particulate matter at 0–1000 μg/mL, the DPPH free radical, and H_2O_2 scavenging activities, reducing power, and metal ion-chelating abilities of SHE were significantly decreased ( p 

  • antioxidant potential of Sargassum horneri extract against urban particulate matter induced oxidation
    Food Science and Biotechnology, 2020
    Co-Authors: Ju Hee Lee, YOUNG HEUN JEE, You-jin Jeon, Hyo-jin Kim, Hyun Jung Kim
    Abstract:

    Particulate matter is a major contribution of air pollution and detrimental to human health. The in vitro antioxidant activities of a brown seaweed, Sargassum horneri ethanol extract (SHE) against particulate matter-induced oxidative stress were investigated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging activity, hydrogen peroxide (H2O2) scavenging activity, superoxide anion (O2− ) inhibition, hydroxyl radical (·OH) scavenging activity, reducing power, and the metal ion-chelating effect. All in vitro antioxidant activities were increased as the concentration of SHE increased (0–1000 μg/mL). When treated with particulate matter at 0–1000 μg/mL, the DPPH free radical, and H2O2 scavenging activities, reducing power, and metal ion-chelating abilities of SHE were significantly decreased (p < 0.05). These results indicate that Sargassum horneri, which is a rich source of bioactive compounds, can be used as a natural source of antioxidants in the food industries.