The Experts below are selected from a list of 110676 Experts worldwide ranked by ideXlab platform
Joaquim M. S. Cabral - One of the best experts on this subject based on the ideXlab platform.
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long term expansion of human induced pluripotent stem cells in a microcarrier based dynamic System
Journal of Chemical Technology & Biotechnology, 2017Co-Authors: Sara M Badenes, Maria Margarida Diogo, Tiago G Fernandes, Claudia C Miranda, Annette Puschklein, Carlos A V Rodrigues, Simone Haupt, Oliver Brüstle, Joaquim M. S. CabralAbstract:BACKGROUND Human induced pluripotent stem (hiPS) cells provide a fascinating tool for exploring disease mechanisms, compound screening in pharmaceutical drug development, and might also represent a renewable source of cells for regenerative medicine applications. This requires increased cell quantities, generated under Good Manufacturing Practice-compatible conditions in a Scalable System. RESULTS A microcarrier-based suspension culture was explored for scaling-up of hiPS cell expansion in serum-free medium using synthetic peptide-acrylate surface microcarriers, developed for long-term support of hiPS cell self-renewal. After a 7 days-culture in spinner flask, cells maintained their typical morphology, pluripotency-associated marker expression and their differentiation capability. Envisaging the improvement of the scalability of the culture, long-term expansion on the microcarriers was attained using confluent microcarriers as the inoculum of successive spinner flask cultures. Importantly, bead-to-bead cell transfer allowed 4 consecutive sub-culture procedures and a cumulative 241-fold expansion was achieved within 15 days, leading to a total viable cell number of 3.3x108 cells. CONCLUSION This work is expected to enable the scale-up of hiPS cell culture under defined conditions and potentially leading to the use of pluripotent stem cell derivatives in cell replacement therapies.
Tim Kelley - One of the best experts on this subject based on the ideXlab platform.
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3d microcarrier System for efficient differentiation of human induced pluripotent stem cells into hematopoietic cells without feeders and serum
Regenerative Medicine, 2013Co-Authors: Shi-jiang Lu, Robert Lanza, Qiang Feng, Tim Kelley, Allen Chen, Shaul Reuveny, Steve OhAbstract:Background: Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential, a feeder-free and Scalable System for large scale production of hESCs and their differentiated derivatives is required. Materials & methods: hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation, the medium for 3D microcarriers was directly switched to EB medium. Results: hESCs on 3D microcarriers maintained pluripotency and formed EBs, which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating, EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore, this 3D System can also be adapted to human induc...
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3d microcarrier System for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum corrected
Regenerative Medicine, 2013Co-Authors: Tim Kelley, Qiang Feng, Shaul Reuveny, Alle Che, Robe LanzaAbstract:Background: Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential, a feeder-free and Scalable System for large scale production of hESCs and their differentiated derivatives is required. Materials & methods: hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation, the medium for 3D microcarriers was directly switched to EB medium. Results: hESCs on 3D microcarriers maintained pluripotency and formed EBs, which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating, EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore, this 3D System can also be adapted to human induc...
Steve Oh - One of the best experts on this subject based on the ideXlab platform.
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3d microcarrier System for efficient differentiation of human induced pluripotent stem cells into hematopoietic cells without feeders and serum
Regenerative Medicine, 2013Co-Authors: Shi-jiang Lu, Robert Lanza, Qiang Feng, Tim Kelley, Allen Chen, Shaul Reuveny, Steve OhAbstract:Background: Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential, a feeder-free and Scalable System for large scale production of hESCs and their differentiated derivatives is required. Materials & methods: hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation, the medium for 3D microcarriers was directly switched to EB medium. Results: hESCs on 3D microcarriers maintained pluripotency and formed EBs, which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating, EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore, this 3D System can also be adapted to human induc...
Oliver Brüstle - One of the best experts on this subject based on the ideXlab platform.
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long term expansion of human induced pluripotent stem cells in a microcarrier based dynamic System
Journal of Chemical Technology & Biotechnology, 2017Co-Authors: Sara M Badenes, Maria Margarida Diogo, Tiago G Fernandes, Claudia C Miranda, Annette Puschklein, Carlos A V Rodrigues, Simone Haupt, Oliver Brüstle, Joaquim M. S. CabralAbstract:BACKGROUND Human induced pluripotent stem (hiPS) cells provide a fascinating tool for exploring disease mechanisms, compound screening in pharmaceutical drug development, and might also represent a renewable source of cells for regenerative medicine applications. This requires increased cell quantities, generated under Good Manufacturing Practice-compatible conditions in a Scalable System. RESULTS A microcarrier-based suspension culture was explored for scaling-up of hiPS cell expansion in serum-free medium using synthetic peptide-acrylate surface microcarriers, developed for long-term support of hiPS cell self-renewal. After a 7 days-culture in spinner flask, cells maintained their typical morphology, pluripotency-associated marker expression and their differentiation capability. Envisaging the improvement of the scalability of the culture, long-term expansion on the microcarriers was attained using confluent microcarriers as the inoculum of successive spinner flask cultures. Importantly, bead-to-bead cell transfer allowed 4 consecutive sub-culture procedures and a cumulative 241-fold expansion was achieved within 15 days, leading to a total viable cell number of 3.3x108 cells. CONCLUSION This work is expected to enable the scale-up of hiPS cell culture under defined conditions and potentially leading to the use of pluripotent stem cell derivatives in cell replacement therapies.
Shaul Reuveny - One of the best experts on this subject based on the ideXlab platform.
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3d microcarrier System for efficient differentiation of human induced pluripotent stem cells into hematopoietic cells without feeders and serum
Regenerative Medicine, 2013Co-Authors: Shi-jiang Lu, Robert Lanza, Qiang Feng, Tim Kelley, Allen Chen, Shaul Reuveny, Steve OhAbstract:Background: Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential, a feeder-free and Scalable System for large scale production of hESCs and their differentiated derivatives is required. Materials & methods: hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation, the medium for 3D microcarriers was directly switched to EB medium. Results: hESCs on 3D microcarriers maintained pluripotency and formed EBs, which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating, EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore, this 3D System can also be adapted to human induc...
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3d microcarrier System for efficient differentiation of human pluripotent stem cells into hematopoietic cells without feeders and serum corrected
Regenerative Medicine, 2013Co-Authors: Tim Kelley, Qiang Feng, Shaul Reuveny, Alle Che, Robe LanzaAbstract:Background: Human embryonic stem cells (hESCs) have been derived and maintained on mouse embryonic fibroblast feeders to keep their undifferentiated status. To realize their clinical potential, a feeder-free and Scalable System for large scale production of hESCs and their differentiated derivatives is required. Materials & methods: hESCs were cultured and passaged on serum/feeder-free 3D microcarriers for five passages. For embryoid body (EB) formation and hemangioblast differentiation, the medium for 3D microcarriers was directly switched to EB medium. Results: hESCs on 3D microcarriers maintained pluripotency and formed EBs, which were ten-times more efficient than hESCs cultured under 2D feeder-free conditions (0.11 ± 0.03 EB cells/hESC input 2D vs 1.19 ± 0.32 EB cells/hESC input 3D). After replating, EB cells from 3D culture readily developed into hemangioblasts with the potential to differentiate into hematopoietic and endothelial cells. Furthermore, this 3D System can also be adapted to human induc...
