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Ana Oleaga - One of the best experts on this subject based on the ideXlab platform.
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Whole genome sequencing and morphological analysis of the human-infecting schistosome emerging in Europe reveals a complex admixture between Schistosoma haematobium and Schistosoma Bovis parasites.
2018Co-Authors: Julien Kincaid Smith, Ana Oleaga, Alan Tracey, Ronaldo De Carvalho Augusto, Ingo Bulla, Nancy Holroyd, Anne Rognon, Olivier Rey, Cristian Chaparro, Santiago Mas-comaAbstract:Schistosomes cause schistosomiasis, the world’s second most important parasitic disease after malaria. A peculiar feature of schistosomes is their ability to produce viable and fertile hybrids. Originally only present in the tropics, schistosomiasis is now also endemic in Europe. Based on two genetic markers the European species had been identified as a hybrid between the ruminant-infective Schistosoma Bovis and the human-infective Schistosoma haematobium.
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Schistosoma Bovis-host interplay: Proteomics for knowing and acting
Molecular and biochemical parasitology, 2016Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Schistosoma Bovis is a parasite of ruminants that causes significant economic losses to farmers throughout Africa, Southwestern Asia and the Mediterranean. Additionally, recent studies have reported its zoonotic potential through the formation of S. Bovis×Schistosoma haematobium hybrids. As observed in the Schistosoma species infecting humans, it is assumed that S. Bovis has also evolved host regulatory molecules that ensure its long-term survival in the bloodstream of its host. Since these molecules could be potential targets for the development of new drugs and anti-schistosome vaccines, their identification and functional characterization were undertaken. With this aim in mind, the molecular interface between S. Bovis and its vertebrate host was subjected to a series of proteomic studies, which started with the analysis of the proteomes of the S. Bovis moieties exposed to the host, namely, the excretory/secretory products and the tegument surface. Thus, a wealth of novel molecular information of S. Bovis was obtained, which in turn allowed the identification of several parasite proteins with fibrinolytic and anticoagulant activities that could be used by S. Bovis to regulate the host defensive systems. Following on, the host interface was investigated by studying the proteome of the host vascular endothelium surface at two points along the infection: in the lung vessels during the schistosomula migration and in the portal vein after the parasites have reached adulthood and sexual maturity. These studies have provided original data regarding the proteomes of the endothelial cell surface of pulmonary vasculature and portal vein in S. Bovis-infected animals, and have shown significant changes in these proteomes associated with infection. This review compiles current information and the analyses of all the proteomic data from S. Bovis and the S. Bovis-host interface, including the molecular and functional characterization of S. Bovis proteins that were found to participate in the regulation of the host coagulation and fibrinolysis systems.
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Proteomic mapping of the lung vascular endothelial cell surface in Schistosoma Bovis-infected hamsters.
Journal of proteomics, 2014Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Abstract Schistosomes are blood trematodes that are perfectly adapted to living in their intravascular habitat and to achieve this they have developed mechanisms enabling them to evade the immune and haemostatic responses of the host and to regulate endothelial cell function to favour their own survival. The objective of this work was to analyse the changes induced by Schistosoma Bovis schistosomula in the proteome expressed by infected hamsters, over 10 and 20 days, on the endothelial surface of their pulmonary vasculature. To accomplish this, we subjected the lungs of non-infected and S. Bovis -infected hamsters to vascular perfusion with a biotin ester reactive. Analysis by liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) of endothelial surface proteins resulted in the identification of a total of 459 non-redundant proteins in the lung vasculature of infected and non-infected hamsters. Here we report the proteins identified, classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters for ten and twenty days. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection. Biological significance To identify the changes induced by schistosomula larvae of Schistosoma Bovis in the proteome of the pulmonary vasculature of the host, we compared the proteins expressed on the vascular endothelium of the lungs of non-infected and infected hamsters over 10 and 20 days. Mass spectrometry analysis (LC–MS/MS) of the proteins isolated from the vascular endothelium resulted in the identification of a total of 459 non-redundant proteins in the lung of infected and non-infected hamsters. The proteins identified are classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection suggesting the possible involvement of these proteins during parasite infection.
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Proteomic identification of endothelial cell surface proteins isolated from the hepatic portal vein of mice infected with Schistosoma Bovis
Journal of proteomics, 2012Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, L. Valero, Ana OleagaAbstract:Abstract Schistosomes are blood parasites adapted to their intravascular habitat that have evolved mechanisms to evade the immune and hemostatic responses of their hosts. It has been observed that the schistosome can regulate the endothelium function along the infection, which contributes to modulation of host defensive responses and parasite survival. The purpose of this work was the analysis of the changes induced by Schistosoma Bovis adult worms in the proteome expressed by infected mice on the endothelial surface of their portal vein. With this aim, we have utilized a methodology that allows the purification, identification and relative quantification of endothelial cell surface proteins after their selective in vivo labeling with biotin. Trypsin digestion of the biotinylated proteins and subsequent liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) resulted in the identification of a total 127 non‐redundant proteins. All these proteins have been classified according to their function and cellular location, and the differences between S. Bovis-infected and non-infected mice in their endothelial surface proteomes have been analyzed. The present work provides the first data on the proteome of the endothelial surface of the portal vein, and identifies some of the changes induced in it after an infection by S. Bovis.
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Molecular cloning, characterization and diagnostic performance of the Schistosoma Bovis 22.6 antigen.
Veterinary parasitology, 2012Co-Authors: Eduardo De La Torre-escudero, Mar Siles-lucas, Raúl Manzano-román, Ricardo Pérez-sánchez, Inmaculada Barrera, Ana OleagaAbstract:Animal schistosomiasis caused by Schistosoma Bovis is a veterinary problem in many areas of the world. It affects a large number of animals and causes important economic losses in livestock production. The 22.6 kDa antigen is a tegumental protein of unknown function, restricted to schistosomes. In S. Bovis it has been identified in the tegument and in an excretion-secretion extract, consisting of several, non-glycosylated isoforms that are recognised by the sera of animals infected with S. Bovis. The aims of the present work were to clone, sequence, express and characterize at molecular level the S. Bovis 22.6 antigen (Sb22.6), as well as to assess the usefulness of the corresponding recombinant protein as a diagnostic antigen in ELISA tests for the detection of free-range cattle farms infested with S. Bovis. Immunolocalization studies revealed that Sb22.6 is expressed in the tegument and some internal tissues of the adult worms, but it is not exposed on the surface of the adult worms and schistosomula. The reactivity of the recombinant Sb22.6 (rSb22.6) in ELISA against antibodies in sera from S. Bovis experimentally infected hamsters and sera from free-range cattle from a S. Bovis endemic area showed that the recombinant protein and the soluble extract of adult worms (SbC) exhibited a similar diagnostic performance. In addition, rSb22.6 did not show cross-reactions with antibodies against Fasciola hepatica, also a frequent trematode parasite in cattle. The rSb22.6 antigen can be readily produced in large amounts and in a highly reproducible fashion, avoiding the types of problem that arise upon using crude extracts such as the SbC. In conclusion, this protein represents a promising epidemiological tool for the surveillance of S. Bovis and may help to implement control measures in the areas and farms were the parasite is present.
Ricardo Pérez-sánchez - One of the best experts on this subject based on the ideXlab platform.
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Schistosoma Bovis-host interplay: Proteomics for knowing and acting
Molecular and biochemical parasitology, 2016Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Schistosoma Bovis is a parasite of ruminants that causes significant economic losses to farmers throughout Africa, Southwestern Asia and the Mediterranean. Additionally, recent studies have reported its zoonotic potential through the formation of S. Bovis×Schistosoma haematobium hybrids. As observed in the Schistosoma species infecting humans, it is assumed that S. Bovis has also evolved host regulatory molecules that ensure its long-term survival in the bloodstream of its host. Since these molecules could be potential targets for the development of new drugs and anti-schistosome vaccines, their identification and functional characterization were undertaken. With this aim in mind, the molecular interface between S. Bovis and its vertebrate host was subjected to a series of proteomic studies, which started with the analysis of the proteomes of the S. Bovis moieties exposed to the host, namely, the excretory/secretory products and the tegument surface. Thus, a wealth of novel molecular information of S. Bovis was obtained, which in turn allowed the identification of several parasite proteins with fibrinolytic and anticoagulant activities that could be used by S. Bovis to regulate the host defensive systems. Following on, the host interface was investigated by studying the proteome of the host vascular endothelium surface at two points along the infection: in the lung vessels during the schistosomula migration and in the portal vein after the parasites have reached adulthood and sexual maturity. These studies have provided original data regarding the proteomes of the endothelial cell surface of pulmonary vasculature and portal vein in S. Bovis-infected animals, and have shown significant changes in these proteomes associated with infection. This review compiles current information and the analyses of all the proteomic data from S. Bovis and the S. Bovis-host interface, including the molecular and functional characterization of S. Bovis proteins that were found to participate in the regulation of the host coagulation and fibrinolysis systems.
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Proteomic mapping of the lung vascular endothelial cell surface in Schistosoma Bovis-infected hamsters.
Journal of proteomics, 2014Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Abstract Schistosomes are blood trematodes that are perfectly adapted to living in their intravascular habitat and to achieve this they have developed mechanisms enabling them to evade the immune and haemostatic responses of the host and to regulate endothelial cell function to favour their own survival. The objective of this work was to analyse the changes induced by Schistosoma Bovis schistosomula in the proteome expressed by infected hamsters, over 10 and 20 days, on the endothelial surface of their pulmonary vasculature. To accomplish this, we subjected the lungs of non-infected and S. Bovis -infected hamsters to vascular perfusion with a biotin ester reactive. Analysis by liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) of endothelial surface proteins resulted in the identification of a total of 459 non-redundant proteins in the lung vasculature of infected and non-infected hamsters. Here we report the proteins identified, classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters for ten and twenty days. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection. Biological significance To identify the changes induced by schistosomula larvae of Schistosoma Bovis in the proteome of the pulmonary vasculature of the host, we compared the proteins expressed on the vascular endothelium of the lungs of non-infected and infected hamsters over 10 and 20 days. Mass spectrometry analysis (LC–MS/MS) of the proteins isolated from the vascular endothelium resulted in the identification of a total of 459 non-redundant proteins in the lung of infected and non-infected hamsters. The proteins identified are classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection suggesting the possible involvement of these proteins during parasite infection.
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Proteomic identification of endothelial cell surface proteins isolated from the hepatic portal vein of mice infected with Schistosoma Bovis
Journal of proteomics, 2012Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, L. Valero, Ana OleagaAbstract:Abstract Schistosomes are blood parasites adapted to their intravascular habitat that have evolved mechanisms to evade the immune and hemostatic responses of their hosts. It has been observed that the schistosome can regulate the endothelium function along the infection, which contributes to modulation of host defensive responses and parasite survival. The purpose of this work was the analysis of the changes induced by Schistosoma Bovis adult worms in the proteome expressed by infected mice on the endothelial surface of their portal vein. With this aim, we have utilized a methodology that allows the purification, identification and relative quantification of endothelial cell surface proteins after their selective in vivo labeling with biotin. Trypsin digestion of the biotinylated proteins and subsequent liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) resulted in the identification of a total 127 non‐redundant proteins. All these proteins have been classified according to their function and cellular location, and the differences between S. Bovis-infected and non-infected mice in their endothelial surface proteomes have been analyzed. The present work provides the first data on the proteome of the endothelial surface of the portal vein, and identifies some of the changes induced in it after an infection by S. Bovis.
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Molecular cloning, characterization and diagnostic performance of the Schistosoma Bovis 22.6 antigen.
Veterinary parasitology, 2012Co-Authors: Eduardo De La Torre-escudero, Mar Siles-lucas, Raúl Manzano-román, Ricardo Pérez-sánchez, Inmaculada Barrera, Ana OleagaAbstract:Animal schistosomiasis caused by Schistosoma Bovis is a veterinary problem in many areas of the world. It affects a large number of animals and causes important economic losses in livestock production. The 22.6 kDa antigen is a tegumental protein of unknown function, restricted to schistosomes. In S. Bovis it has been identified in the tegument and in an excretion-secretion extract, consisting of several, non-glycosylated isoforms that are recognised by the sera of animals infected with S. Bovis. The aims of the present work were to clone, sequence, express and characterize at molecular level the S. Bovis 22.6 antigen (Sb22.6), as well as to assess the usefulness of the corresponding recombinant protein as a diagnostic antigen in ELISA tests for the detection of free-range cattle farms infested with S. Bovis. Immunolocalization studies revealed that Sb22.6 is expressed in the tegument and some internal tissues of the adult worms, but it is not exposed on the surface of the adult worms and schistosomula. The reactivity of the recombinant Sb22.6 (rSb22.6) in ELISA against antibodies in sera from S. Bovis experimentally infected hamsters and sera from free-range cattle from a S. Bovis endemic area showed that the recombinant protein and the soluble extract of adult worms (SbC) exhibited a similar diagnostic performance. In addition, rSb22.6 did not show cross-reactions with antibodies against Fasciola hepatica, also a frequent trematode parasite in cattle. The rSb22.6 antigen can be readily produced in large amounts and in a highly reproducible fashion, avoiding the types of problem that arise upon using crude extracts such as the SbC. In conclusion, this protein represents a promising epidemiological tool for the surveillance of S. Bovis and may help to implement control measures in the areas and farms were the parasite is present.
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Molecular and functional characterization of a Schistosoma Bovis annexin: fibrinolytic and anticoagulant activity.
Veterinary parasitology, 2011Co-Authors: Eduardo De La Torre-escudero, Mar Siles-lucas, Raúl Manzano-román, Ricardo Pérez-sánchez, J. Carlos Moyano, Inmaculada Barrera, Ana OleagaAbstract:Annexins belong to an evolutionarily conserved multigene family of proteins expressed throughout the animal and plant kingdoms. Although they are soluble cytosolic proteins that lack signal sequences, they have also been detected in extracellular fluids and have been associated with cell surface membranes, where they could be involved in anti-haemostatic and anti-inflammatory functions. Schistosome annexins have been identified on the parasite's tegument surface and excretory/secretory products, but their functions are still unknown. Here we report the cloning, sequencing, in silico analysis, and functional characterization of a Schistosoma Bovis annexin. The predicted protein has typical annexin secondary and tertiary structures. Bioassays with the recombinant protein revealed that the protein is biologically active in vitro, showing fibrinolytic and anticoagulant properties. Finally, the expression of the native protein on the tegument surface of S. Bovis schistosomula and adult worms is demonstrated, revealing the possibility of exposure to the host's immune system and thus offering a potential vaccine target for the control of schistosomiasis in ruminants.
Eduardo De La Torre-escudero - One of the best experts on this subject based on the ideXlab platform.
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Schistosoma Bovis-host interplay: Proteomics for knowing and acting
Molecular and biochemical parasitology, 2016Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Schistosoma Bovis is a parasite of ruminants that causes significant economic losses to farmers throughout Africa, Southwestern Asia and the Mediterranean. Additionally, recent studies have reported its zoonotic potential through the formation of S. Bovis×Schistosoma haematobium hybrids. As observed in the Schistosoma species infecting humans, it is assumed that S. Bovis has also evolved host regulatory molecules that ensure its long-term survival in the bloodstream of its host. Since these molecules could be potential targets for the development of new drugs and anti-schistosome vaccines, their identification and functional characterization were undertaken. With this aim in mind, the molecular interface between S. Bovis and its vertebrate host was subjected to a series of proteomic studies, which started with the analysis of the proteomes of the S. Bovis moieties exposed to the host, namely, the excretory/secretory products and the tegument surface. Thus, a wealth of novel molecular information of S. Bovis was obtained, which in turn allowed the identification of several parasite proteins with fibrinolytic and anticoagulant activities that could be used by S. Bovis to regulate the host defensive systems. Following on, the host interface was investigated by studying the proteome of the host vascular endothelium surface at two points along the infection: in the lung vessels during the schistosomula migration and in the portal vein after the parasites have reached adulthood and sexual maturity. These studies have provided original data regarding the proteomes of the endothelial cell surface of pulmonary vasculature and portal vein in S. Bovis-infected animals, and have shown significant changes in these proteomes associated with infection. This review compiles current information and the analyses of all the proteomic data from S. Bovis and the S. Bovis-host interface, including the molecular and functional characterization of S. Bovis proteins that were found to participate in the regulation of the host coagulation and fibrinolysis systems.
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Proteomic mapping of the lung vascular endothelial cell surface in Schistosoma Bovis-infected hamsters.
Journal of proteomics, 2014Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Abstract Schistosomes are blood trematodes that are perfectly adapted to living in their intravascular habitat and to achieve this they have developed mechanisms enabling them to evade the immune and haemostatic responses of the host and to regulate endothelial cell function to favour their own survival. The objective of this work was to analyse the changes induced by Schistosoma Bovis schistosomula in the proteome expressed by infected hamsters, over 10 and 20 days, on the endothelial surface of their pulmonary vasculature. To accomplish this, we subjected the lungs of non-infected and S. Bovis -infected hamsters to vascular perfusion with a biotin ester reactive. Analysis by liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) of endothelial surface proteins resulted in the identification of a total of 459 non-redundant proteins in the lung vasculature of infected and non-infected hamsters. Here we report the proteins identified, classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters for ten and twenty days. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection. Biological significance To identify the changes induced by schistosomula larvae of Schistosoma Bovis in the proteome of the pulmonary vasculature of the host, we compared the proteins expressed on the vascular endothelium of the lungs of non-infected and infected hamsters over 10 and 20 days. Mass spectrometry analysis (LC–MS/MS) of the proteins isolated from the vascular endothelium resulted in the identification of a total of 459 non-redundant proteins in the lung of infected and non-infected hamsters. The proteins identified are classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection suggesting the possible involvement of these proteins during parasite infection.
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Proteomic mapping of the lung vascular endothelial cell surface in Schistosoma Bovis-infected hamsters
'Elsevier BV', 2014Co-Authors: Eduardo De La Torre-escudero, Pérez Sánchez Ricardo, Manzano Román Raúl, Oleaga AnaAbstract:46 páginas, 6 figuras, 3 tablas.--The definitive version is available at http://www.elsevier.comSchistosomes are blood trematodes that are perfectly adapted to living in their intravascular habitat and to achieve this they have developed mechanisms enabling them to evade the immune and haemostatic responses of the host and to regulate endothelial cell function to favour their own survival. The objective of this work was to analyse the changes induced by Schistosoma Bovis schistosomula in the proteome expressed by infected hamsters, over 10 and 20 days, on the endothelial surface of their pulmonary vasculature. To accomplish this, we subjected the lungs of non-infected and S. Bovis-infected hamsters to vascular perfusion with a biotin ester reactive. Analysis by liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) of endothelial surface proteins resulted in the identification of a total of 459 non-redundant proteins in the lung vasculature of infected and non-infected hamsters. Here we report the proteins identified, classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis-infected hamsters for ten and twenty days. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection.This research was funded by project AGL2010- 597 18163 granted by the Spanish Ministry of Science and Innovation.Peer reviewe
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Proteomic identification of endothelial cell surface proteins isolated from the hepatic portal vein of mice infected with Schistosoma Bovis
Journal of proteomics, 2012Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, L. Valero, Ana OleagaAbstract:Abstract Schistosomes are blood parasites adapted to their intravascular habitat that have evolved mechanisms to evade the immune and hemostatic responses of their hosts. It has been observed that the schistosome can regulate the endothelium function along the infection, which contributes to modulation of host defensive responses and parasite survival. The purpose of this work was the analysis of the changes induced by Schistosoma Bovis adult worms in the proteome expressed by infected mice on the endothelial surface of their portal vein. With this aim, we have utilized a methodology that allows the purification, identification and relative quantification of endothelial cell surface proteins after their selective in vivo labeling with biotin. Trypsin digestion of the biotinylated proteins and subsequent liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) resulted in the identification of a total 127 non‐redundant proteins. All these proteins have been classified according to their function and cellular location, and the differences between S. Bovis-infected and non-infected mice in their endothelial surface proteomes have been analyzed. The present work provides the first data on the proteome of the endothelial surface of the portal vein, and identifies some of the changes induced in it after an infection by S. Bovis.
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Molecular cloning, characterization and diagnostic performance of the Schistosoma Bovis 22.6 antigen.
Veterinary parasitology, 2012Co-Authors: Eduardo De La Torre-escudero, Mar Siles-lucas, Raúl Manzano-román, Ricardo Pérez-sánchez, Inmaculada Barrera, Ana OleagaAbstract:Animal schistosomiasis caused by Schistosoma Bovis is a veterinary problem in many areas of the world. It affects a large number of animals and causes important economic losses in livestock production. The 22.6 kDa antigen is a tegumental protein of unknown function, restricted to schistosomes. In S. Bovis it has been identified in the tegument and in an excretion-secretion extract, consisting of several, non-glycosylated isoforms that are recognised by the sera of animals infected with S. Bovis. The aims of the present work were to clone, sequence, express and characterize at molecular level the S. Bovis 22.6 antigen (Sb22.6), as well as to assess the usefulness of the corresponding recombinant protein as a diagnostic antigen in ELISA tests for the detection of free-range cattle farms infested with S. Bovis. Immunolocalization studies revealed that Sb22.6 is expressed in the tegument and some internal tissues of the adult worms, but it is not exposed on the surface of the adult worms and schistosomula. The reactivity of the recombinant Sb22.6 (rSb22.6) in ELISA against antibodies in sera from S. Bovis experimentally infected hamsters and sera from free-range cattle from a S. Bovis endemic area showed that the recombinant protein and the soluble extract of adult worms (SbC) exhibited a similar diagnostic performance. In addition, rSb22.6 did not show cross-reactions with antibodies against Fasciola hepatica, also a frequent trematode parasite in cattle. The rSb22.6 antigen can be readily produced in large amounts and in a highly reproducible fashion, avoiding the types of problem that arise upon using crude extracts such as the SbC. In conclusion, this protein represents a promising epidemiological tool for the surveillance of S. Bovis and may help to implement control measures in the areas and farms were the parasite is present.
Raúl Manzano-román - One of the best experts on this subject based on the ideXlab platform.
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Schistosoma Bovis-host interplay: Proteomics for knowing and acting
Molecular and biochemical parasitology, 2016Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Schistosoma Bovis is a parasite of ruminants that causes significant economic losses to farmers throughout Africa, Southwestern Asia and the Mediterranean. Additionally, recent studies have reported its zoonotic potential through the formation of S. Bovis×Schistosoma haematobium hybrids. As observed in the Schistosoma species infecting humans, it is assumed that S. Bovis has also evolved host regulatory molecules that ensure its long-term survival in the bloodstream of its host. Since these molecules could be potential targets for the development of new drugs and anti-schistosome vaccines, their identification and functional characterization were undertaken. With this aim in mind, the molecular interface between S. Bovis and its vertebrate host was subjected to a series of proteomic studies, which started with the analysis of the proteomes of the S. Bovis moieties exposed to the host, namely, the excretory/secretory products and the tegument surface. Thus, a wealth of novel molecular information of S. Bovis was obtained, which in turn allowed the identification of several parasite proteins with fibrinolytic and anticoagulant activities that could be used by S. Bovis to regulate the host defensive systems. Following on, the host interface was investigated by studying the proteome of the host vascular endothelium surface at two points along the infection: in the lung vessels during the schistosomula migration and in the portal vein after the parasites have reached adulthood and sexual maturity. These studies have provided original data regarding the proteomes of the endothelial cell surface of pulmonary vasculature and portal vein in S. Bovis-infected animals, and have shown significant changes in these proteomes associated with infection. This review compiles current information and the analyses of all the proteomic data from S. Bovis and the S. Bovis-host interface, including the molecular and functional characterization of S. Bovis proteins that were found to participate in the regulation of the host coagulation and fibrinolysis systems.
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Proteomic mapping of the lung vascular endothelial cell surface in Schistosoma Bovis-infected hamsters.
Journal of proteomics, 2014Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, Ana OleagaAbstract:Abstract Schistosomes are blood trematodes that are perfectly adapted to living in their intravascular habitat and to achieve this they have developed mechanisms enabling them to evade the immune and haemostatic responses of the host and to regulate endothelial cell function to favour their own survival. The objective of this work was to analyse the changes induced by Schistosoma Bovis schistosomula in the proteome expressed by infected hamsters, over 10 and 20 days, on the endothelial surface of their pulmonary vasculature. To accomplish this, we subjected the lungs of non-infected and S. Bovis -infected hamsters to vascular perfusion with a biotin ester reactive. Analysis by liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) of endothelial surface proteins resulted in the identification of a total of 459 non-redundant proteins in the lung vasculature of infected and non-infected hamsters. Here we report the proteins identified, classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters for ten and twenty days. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection. Biological significance To identify the changes induced by schistosomula larvae of Schistosoma Bovis in the proteome of the pulmonary vasculature of the host, we compared the proteins expressed on the vascular endothelium of the lungs of non-infected and infected hamsters over 10 and 20 days. Mass spectrometry analysis (LC–MS/MS) of the proteins isolated from the vascular endothelium resulted in the identification of a total of 459 non-redundant proteins in the lung of infected and non-infected hamsters. The proteins identified are classified according to their biological function and cellular location, further analysing the differences in lung vascular proteomes between non-infected and S. Bovis -infected hamsters. This work provides the first data on the vascular surface proteome of the lung after S. Bovis infection and identifies some of the changes induced in it during infection suggesting the possible involvement of these proteins during parasite infection.
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Proteomic identification of endothelial cell surface proteins isolated from the hepatic portal vein of mice infected with Schistosoma Bovis
Journal of proteomics, 2012Co-Authors: Eduardo De La Torre-escudero, Raúl Manzano-román, Ricardo Pérez-sánchez, L. Valero, Ana OleagaAbstract:Abstract Schistosomes are blood parasites adapted to their intravascular habitat that have evolved mechanisms to evade the immune and hemostatic responses of their hosts. It has been observed that the schistosome can regulate the endothelium function along the infection, which contributes to modulation of host defensive responses and parasite survival. The purpose of this work was the analysis of the changes induced by Schistosoma Bovis adult worms in the proteome expressed by infected mice on the endothelial surface of their portal vein. With this aim, we have utilized a methodology that allows the purification, identification and relative quantification of endothelial cell surface proteins after their selective in vivo labeling with biotin. Trypsin digestion of the biotinylated proteins and subsequent liquid chromatography and tandem mass spectrometry analysis (LC–MS/MS) resulted in the identification of a total 127 non‐redundant proteins. All these proteins have been classified according to their function and cellular location, and the differences between S. Bovis-infected and non-infected mice in their endothelial surface proteomes have been analyzed. The present work provides the first data on the proteome of the endothelial surface of the portal vein, and identifies some of the changes induced in it after an infection by S. Bovis.
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Molecular cloning, characterization and diagnostic performance of the Schistosoma Bovis 22.6 antigen.
Veterinary parasitology, 2012Co-Authors: Eduardo De La Torre-escudero, Mar Siles-lucas, Raúl Manzano-román, Ricardo Pérez-sánchez, Inmaculada Barrera, Ana OleagaAbstract:Animal schistosomiasis caused by Schistosoma Bovis is a veterinary problem in many areas of the world. It affects a large number of animals and causes important economic losses in livestock production. The 22.6 kDa antigen is a tegumental protein of unknown function, restricted to schistosomes. In S. Bovis it has been identified in the tegument and in an excretion-secretion extract, consisting of several, non-glycosylated isoforms that are recognised by the sera of animals infected with S. Bovis. The aims of the present work were to clone, sequence, express and characterize at molecular level the S. Bovis 22.6 antigen (Sb22.6), as well as to assess the usefulness of the corresponding recombinant protein as a diagnostic antigen in ELISA tests for the detection of free-range cattle farms infested with S. Bovis. Immunolocalization studies revealed that Sb22.6 is expressed in the tegument and some internal tissues of the adult worms, but it is not exposed on the surface of the adult worms and schistosomula. The reactivity of the recombinant Sb22.6 (rSb22.6) in ELISA against antibodies in sera from S. Bovis experimentally infected hamsters and sera from free-range cattle from a S. Bovis endemic area showed that the recombinant protein and the soluble extract of adult worms (SbC) exhibited a similar diagnostic performance. In addition, rSb22.6 did not show cross-reactions with antibodies against Fasciola hepatica, also a frequent trematode parasite in cattle. The rSb22.6 antigen can be readily produced in large amounts and in a highly reproducible fashion, avoiding the types of problem that arise upon using crude extracts such as the SbC. In conclusion, this protein represents a promising epidemiological tool for the surveillance of S. Bovis and may help to implement control measures in the areas and farms were the parasite is present.
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Molecular and functional characterization of a Schistosoma Bovis annexin: fibrinolytic and anticoagulant activity.
Veterinary parasitology, 2011Co-Authors: Eduardo De La Torre-escudero, Mar Siles-lucas, Raúl Manzano-román, Ricardo Pérez-sánchez, J. Carlos Moyano, Inmaculada Barrera, Ana OleagaAbstract:Annexins belong to an evolutionarily conserved multigene family of proteins expressed throughout the animal and plant kingdoms. Although they are soluble cytosolic proteins that lack signal sequences, they have also been detected in extracellular fluids and have been associated with cell surface membranes, where they could be involved in anti-haemostatic and anti-inflammatory functions. Schistosome annexins have been identified on the parasite's tegument surface and excretory/secretory products, but their functions are still unknown. Here we report the cloning, sequencing, in silico analysis, and functional characterization of a Schistosoma Bovis annexin. The predicted protein has typical annexin secondary and tertiary structures. Bioassays with the recombinant protein revealed that the protein is biologically active in vitro, showing fibrinolytic and anticoagulant properties. Finally, the expression of the native protein on the tegument surface of S. Bovis schistosomula and adult worms is demonstrated, revealing the possibility of exposure to the host's immune system and thus offering a potential vaccine target for the control of schistosomiasis in ruminants.
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Barcoding hybrids: heterogeneous distribution of Schistosoma haematobium × Schistosoma Bovis hybrids across the Senegal River Basin
Parasitology, 2018Co-Authors: Nele Boon, Frederik Van Den Broeck, Djiby Faye, Filip Volckaert, Souleymane Mboup, Katja Polman, Tine HuyseAbstract:Hybridization events between Schistosoma species (Digenea, Platyhelminthes) are reported with increasing frequency, largely due to improved access to molecular tools. Nevertheless, little is known about the distribution and frequency of hybrid schistosomes in nature. Screening for hybrids on a large scale is complicated by the need for nuclear and mitochondrial sequence information, precluding a 'simple' barcoding approach. Here we aimed to determine and understand the spatiotemporal distribution of Schistosoma haematobium × Schistosoma Bovis hybrids in the Senegal River Basin. From ten villages, distributed over the four main water basins, we genotyped a total of 1236 schistosome larvae collected from human urine samples using a partial mitochondrial cox1 fragment; a subset of 268 parasites was also genotyped using ITS rDNA. Hybrid schistosomes were unevenly distributed, with substantially higher numbers in villages bordering Lac de Guiers than in villages from the Lampsar River and the Middle Valley of the Senegal River. The frequency of hybrids per village was not linked with the prevalence of urinary schistosomiasis in that village. However, we did find a significant positive association between the frequency of hybrids per village and the prevalence of Schistosoma mansoni. We discuss the potential consequences of adopting a barcoding approach when studying hybrids in nature.
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barcoding hybrids heterogeneous distribution of Schistosoma haematobium Schistosoma Bovis hybrids across the senegal river basin
Parasitology, 2018Co-Authors: Nele Boon, Frederik Van Den Broeck, Djiby Faye, Filip Volckaert, Souleymane Mboup, Katja Polman, Tine HuyseAbstract:Hybridization events between Schistosoma species (Digenea, Platyhelminthes) are reported with increasing frequency, largely due to improved access to molecular tools. Nevertheless, little is known about the distribution and frequency of hybrid schistosomes in nature. Screening for hybrids on a large scale is complicated by the need for nuclear and mitochondrial sequence information, precluding a 'simple' barcoding approach. Here we aimed to determine and understand the spatiotemporal distribution of Schistosoma haematobium × Schistosoma Bovis hybrids in the Senegal River Basin. From ten villages, distributed over the four main water basins, we genotyped a total of 1236 schistosome larvae collected from human urine samples using a partial mitochondrial cox1 fragment; a subset of 268 parasites was also genotyped using ITS rDNA. Hybrid schistosomes were unevenly distributed, with substantially higher numbers in villages bordering Lac de Guiers than in villages from the Lampsar River and the Middle Valley of the Senegal River. The frequency of hybrids per village was not linked with the prevalence of urinary schistosomiasis in that village. However, we did find a significant positive association between the frequency of hybrids per village and the prevalence of Schistosoma mansoni. We discuss the potential consequences of adopting a barcoding approach when studying hybrids in nature.
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Diagnosis and Clinical Management of Schistosoma haematobium-Schistosoma Bovis Hybrid Infection in a Cluster of Travelers Returning From Mali.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 2016Co-Authors: Patrick Soentjens, Lieselotte Cnops, Tine Huyse, Cedric P. Yansouni, Daniel De Vos, Emmanuel Bottieau, Jan Clerinx, Marjan Van EsbroeckAbstract:Ten Belgian travelers returned from Mali with a Schistosoma haematobium-Schistosoma Bovis hybrid infection, confirmed by DNA sequencing from eggs. Clinical symptoms and laboratory findings resembled those of classic acute schistosomiasis, but the detected eggs were morphologically unusual.
