The Experts below are selected from a list of 14235 Experts worldwide ranked by ideXlab platform
Yamei Jin - One of the best experts on this subject based on the ideXlab platform.
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Screening interaction protein of Schistosoma Japonicum gynecophoral canal protein by phage display
Chinese journal of schistosomiasis control, 2011Co-Authors: He Jt, Shi Yl, Liu Pp, Jiaojiao Lin, Liu Jm, Shi Yj, Yamei JinAbstract:OBJECTIVE: To search the interaction protein of Schistosoma Japonicum gynecophoral canal protein (SjGCP). METHODS: The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma Japonicum. RESULTS: A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. CONCLUSION; Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.
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Screening interaction protein of Schistosoma Japonicum gynecophoral canal protein by phage display
Chinese journal of schistosomiasis control, 2011Co-Authors: He Jt, Shi Yl, Liu Pp, Jiaojiao Lin, Liu Jm, Shi Yj, Yamei JinAbstract:OBJECTIVE: To search the interaction protein of Schistosoma Japonicum gynecophoral canal protein (SjGCP). METHODS: The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma Japonicum. RESULTS: A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. CONCLUSION; Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.
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Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma Japonicum gene
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2003Co-Authors: Liang Zhang, Yamei Jin, Guofeng Cheng, Xingang Feng, Chunxiu Yuan, You-min Cai, Jiaojiao LinAbstract:Based on the phenomenon of the natural anti-schistosomiasis in Microtus fortis, the sera from normal Microtus fortis were employed to immunoscreen the cDNA library of adult Schistosoma Japonicum (Chinese strain), two positive clones were obtained, RACE technique was further applied to amplify one of the clones, and a cDNA fragment with an ORF was identified. Sequencing revealed that it was a novel gene of Schistosoma Japonicum, and it was named SjMF4 (Schistosoma Japonicum Microtus fortis 4). Then the structure and functional motifs of SjMF4 were analysed. The gene was subcloned into pET-28a(+) vector; the recombinant protein showed good antigenicity in Western blotting. The gene was further subcloned into eukaryotic expression vector pcDNA3 to construct the DNA vaccine containing SjMF4. Immune experiments in mice showed significant protection that the recombinant plasmid did induce 28.64%+/-3.82% worm reduction and 21.73%+/-3.98% egg reduction than controls against the Schistosoma Japonicum cercaria challenge.
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Cloning and expression of 21.7 kD protein gene of Schistosoma Japonicum (Chinese strain)
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2002Co-Authors: Yamei Jin, Jiaojiao Lin, Liang Zhang, You-min CaiAbstract:A 558 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma Japonicum(Chinese strain) mRNA with a pair of primers that were designed according to published Sj21.7p gene encoding 21.7 kD protein of Schistosoma Japonicum(Philippines strain). Sequence analysis indicated that this frame, named Sj21.7 (Ch), with 99% homology to Sj21.7 p, contained a complete open reading fragment (ORF) of 21.7 kD protein gene of Schistosoma Japonicum(Chinese strain). The amino acid sequence shared 98% homology with 21.7 kD protein of Schistosoma Japonicum. This fragment was cloned into the expression vector pET28a (+) and subsequently expressed in Escherichia coli with IPTG induction. SDS-PAGE analysis revealed that the molecular weight of this expressed product was 25.4 kD. Western blotting showed that the recombinant protein reacted well with the rabbit serum immunized with Sj worm antigen, indicating that this expressed product had good antigenicity.
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Cloning and expression of gynecophoral canal protein gene of Schistosoma Japonicum (Chinese strain)
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2002Co-Authors: Yamei Jin, Jiaojiao Lin, Liang Zhang, Xiangling Feng, Yuan-cong Zhou, You-min CaiAbstract:A 1949 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma Japonicum (Chinese strain) mRNA with 3 pair of primers that were designed according to published SmGCP gene encoding gynecophoral canal protein of Schistosoma mansoni and SjGCP1 gene encoding the conservative region of gynecophoral canal protein of Schistosoma Japonicum. Sequence analysis indicated that this fragment, named SjGCP, with 85% identity to SmGCP, contained a complete open reading frame (ORF) of gynecophoral canal protein gene of Schistosoma Japonicum (Chinese strain). The amino acid sequence shared 83.7% identity with gynecophoral canal protein of Schistosoma mansoni. This fragment was cloned into the expression vector pET28c(+) and subsequently expressed in Escherichia coli. SDS-PAGE revealed that the molecular weight of this expressed product was 80 kD. Western blotting showed that the recombinant protein reacted well with the rabbit serum immunized with Sj worm antigen, indicating that this expressed product had good antigenicity.
Jiaojiao Lin - One of the best experts on this subject based on the ideXlab platform.
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Screening interaction protein of Schistosoma Japonicum gynecophoral canal protein by phage display
Chinese journal of schistosomiasis control, 2011Co-Authors: He Jt, Shi Yl, Liu Pp, Jiaojiao Lin, Liu Jm, Shi Yj, Yamei JinAbstract:OBJECTIVE: To search the interaction protein of Schistosoma Japonicum gynecophoral canal protein (SjGCP). METHODS: The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma Japonicum. RESULTS: A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. CONCLUSION; Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.
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Screening interaction protein of Schistosoma Japonicum gynecophoral canal protein by phage display
Chinese journal of schistosomiasis control, 2011Co-Authors: He Jt, Shi Yl, Liu Pp, Jiaojiao Lin, Liu Jm, Shi Yj, Yamei JinAbstract:OBJECTIVE: To search the interaction protein of Schistosoma Japonicum gynecophoral canal protein (SjGCP). METHODS: The recombinant rSjGCP was used as a target to search the T7 phage display cDNA library from 44-day adult Schistosoma Japonicum. RESULTS: A total of 70 ESTs were obtained. The bioinformatics analyses to the screening results revealed that 5 interaction proteins or peptides of SjGCP were found. CONCLUSION; Interaction proteins or peptides of SjGCP are successfully obtained through screening the T7 phage display library.
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Construction and expression of recombinant baculovirus with Schistosoma Japonicum SjPP gene
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2008Co-Authors: Li-xiao Yao, You-min Cai, Li-hong Tao, Guan-zhen Yang, Jiaojiao LinAbstract:Objective To express the soluble recombinant Schistosoma Japonicum SjPP proteins in TN5B1-4 cells. Methods The total RNA was extracted from adult worms of Schistosoma Japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting. Results The infective recombinant bac-ulovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells. Conclusion The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.
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Gene difference expression between mice and {\sl Microtus fortis} infected with {\sl Schistosoma Japonicum} using cDNA microarrays
2004Co-Authors: Jun Sun, Jiaojiao Lin, Guofeng Cheng, You-min Cai, Yaojun Shi, Hongbo JiangAbstract:Gene difference expression of the lung tissues between of mice and Microtus fortis infected with Schistosoma Japonicum for 7?d, and the lung tissues and the liver tissues of Microtus fortis infected with Schistosoma Japonicum for 12?d were analyzed by cDNA microarrays. The results showed that serine protease inhibitor genes expression was up regulated and immune associated genes expression were not obviously changed in the lung tissues of mice. On the contrary, serine protease inhibitor genes expressed constantly in the lung tissues of Microtus fortis infected with Schistosoma Japonicum for 7?d and Several non specific immune associated genes (such as lysozyme gene and cathepsin genes) were up regulated. At the same time, some important specific immune associated genes(such as CD74、MHC Ⅱ and MHC Ⅰ) were up regulated. The lung tissues and liver tissues of Microtus fortis infected with Schistosoma Japonicum for 12?d also confirmed the results by enhancing expression of the non specific immune associated genes and specific immune associated genes. Furthermore, in the lung tissues of Microtus fortis infected with Schistosoma Japonicum for 12?d serine protease inhibitor genes expression were down regulated. It was suggested that the mice and Microtus fortis share reverse expression patterns, which was helpful to deeply understand the molecular mechanism of natural resistance of Microtus fortis to Schistosoma Japonicum .
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Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma Japonicum gene
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2003Co-Authors: Liang Zhang, Yamei Jin, Guofeng Cheng, Xingang Feng, Chunxiu Yuan, You-min Cai, Jiaojiao LinAbstract:Based on the phenomenon of the natural anti-schistosomiasis in Microtus fortis, the sera from normal Microtus fortis were employed to immunoscreen the cDNA library of adult Schistosoma Japonicum (Chinese strain), two positive clones were obtained, RACE technique was further applied to amplify one of the clones, and a cDNA fragment with an ORF was identified. Sequencing revealed that it was a novel gene of Schistosoma Japonicum, and it was named SjMF4 (Schistosoma Japonicum Microtus fortis 4). Then the structure and functional motifs of SjMF4 were analysed. The gene was subcloned into pET-28a(+) vector; the recombinant protein showed good antigenicity in Western blotting. The gene was further subcloned into eukaryotic expression vector pcDNA3 to construct the DNA vaccine containing SjMF4. Immune experiments in mice showed significant protection that the recombinant plasmid did induce 28.64%+/-3.82% worm reduction and 21.73%+/-3.98% egg reduction than controls against the Schistosoma Japonicum cercaria challenge.
You-min Cai - One of the best experts on this subject based on the ideXlab platform.
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Construction and expression of recombinant baculovirus with Schistosoma Japonicum SjPP gene
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2008Co-Authors: Li-xiao Yao, You-min Cai, Li-hong Tao, Guan-zhen Yang, Jiaojiao LinAbstract:Objective To express the soluble recombinant Schistosoma Japonicum SjPP proteins in TN5B1-4 cells. Methods The total RNA was extracted from adult worms of Schistosoma Japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting. Results The infective recombinant bac-ulovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells. Conclusion The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.
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Gene difference expression between mice and {\sl Microtus fortis} infected with {\sl Schistosoma Japonicum} using cDNA microarrays
2004Co-Authors: Jun Sun, Jiaojiao Lin, Guofeng Cheng, You-min Cai, Yaojun Shi, Hongbo JiangAbstract:Gene difference expression of the lung tissues between of mice and Microtus fortis infected with Schistosoma Japonicum for 7?d, and the lung tissues and the liver tissues of Microtus fortis infected with Schistosoma Japonicum for 12?d were analyzed by cDNA microarrays. The results showed that serine protease inhibitor genes expression was up regulated and immune associated genes expression were not obviously changed in the lung tissues of mice. On the contrary, serine protease inhibitor genes expressed constantly in the lung tissues of Microtus fortis infected with Schistosoma Japonicum for 7?d and Several non specific immune associated genes (such as lysozyme gene and cathepsin genes) were up regulated. At the same time, some important specific immune associated genes(such as CD74、MHC Ⅱ and MHC Ⅰ) were up regulated. The lung tissues and liver tissues of Microtus fortis infected with Schistosoma Japonicum for 12?d also confirmed the results by enhancing expression of the non specific immune associated genes and specific immune associated genes. Furthermore, in the lung tissues of Microtus fortis infected with Schistosoma Japonicum for 12?d serine protease inhibitor genes expression were down regulated. It was suggested that the mice and Microtus fortis share reverse expression patterns, which was helpful to deeply understand the molecular mechanism of natural resistance of Microtus fortis to Schistosoma Japonicum .
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Cloning, expressing and functional analysis of SjMF4, a novel Schistosoma Japonicum gene
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2003Co-Authors: Liang Zhang, Yamei Jin, Guofeng Cheng, Xingang Feng, Chunxiu Yuan, You-min Cai, Jiaojiao LinAbstract:Based on the phenomenon of the natural anti-schistosomiasis in Microtus fortis, the sera from normal Microtus fortis were employed to immunoscreen the cDNA library of adult Schistosoma Japonicum (Chinese strain), two positive clones were obtained, RACE technique was further applied to amplify one of the clones, and a cDNA fragment with an ORF was identified. Sequencing revealed that it was a novel gene of Schistosoma Japonicum, and it was named SjMF4 (Schistosoma Japonicum Microtus fortis 4). Then the structure and functional motifs of SjMF4 were analysed. The gene was subcloned into pET-28a(+) vector; the recombinant protein showed good antigenicity in Western blotting. The gene was further subcloned into eukaryotic expression vector pcDNA3 to construct the DNA vaccine containing SjMF4. Immune experiments in mice showed significant protection that the recombinant plasmid did induce 28.64%+/-3.82% worm reduction and 21.73%+/-3.98% egg reduction than controls against the Schistosoma Japonicum cercaria challenge.
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Cloning and expression of 21.7 kD protein gene of Schistosoma Japonicum (Chinese strain)
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2002Co-Authors: Yamei Jin, Jiaojiao Lin, Liang Zhang, You-min CaiAbstract:A 558 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma Japonicum(Chinese strain) mRNA with a pair of primers that were designed according to published Sj21.7p gene encoding 21.7 kD protein of Schistosoma Japonicum(Philippines strain). Sequence analysis indicated that this frame, named Sj21.7 (Ch), with 99% homology to Sj21.7 p, contained a complete open reading fragment (ORF) of 21.7 kD protein gene of Schistosoma Japonicum(Chinese strain). The amino acid sequence shared 98% homology with 21.7 kD protein of Schistosoma Japonicum. This fragment was cloned into the expression vector pET28a (+) and subsequently expressed in Escherichia coli with IPTG induction. SDS-PAGE analysis revealed that the molecular weight of this expressed product was 25.4 kD. Western blotting showed that the recombinant protein reacted well with the rabbit serum immunized with Sj worm antigen, indicating that this expressed product had good antigenicity.
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Cloning and expression of gynecophoral canal protein gene of Schistosoma Japonicum (Chinese strain)
Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 2002Co-Authors: Yamei Jin, Jiaojiao Lin, Liang Zhang, Xiangling Feng, Yuan-cong Zhou, You-min CaiAbstract:A 1949 bp cDNA fragment was amplified by RT-PCR from adult Schistosoma Japonicum (Chinese strain) mRNA with 3 pair of primers that were designed according to published SmGCP gene encoding gynecophoral canal protein of Schistosoma mansoni and SjGCP1 gene encoding the conservative region of gynecophoral canal protein of Schistosoma Japonicum. Sequence analysis indicated that this fragment, named SjGCP, with 85% identity to SmGCP, contained a complete open reading frame (ORF) of gynecophoral canal protein gene of Schistosoma Japonicum (Chinese strain). The amino acid sequence shared 83.7% identity with gynecophoral canal protein of Schistosoma mansoni. This fragment was cloned into the expression vector pET28c(+) and subsequently expressed in Escherichia coli. SDS-PAGE revealed that the molecular weight of this expressed product was 80 kD. Western blotting showed that the recombinant protein reacted well with the rabbit serum immunized with Sj worm antigen, indicating that this expressed product had good antigenicity.
Gao Dongmei - One of the best experts on this subject based on the ideXlab platform.
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Construction and screening of phage display single-chain Fv antibody library against UV-attenuated Schistosoma Japonicum cercariae.
Journal of Tropical Medicine, 2010Co-Authors: Zhou Shuaifeng, Wang Shiping, He Zhuo, Li Lin, Gao DongmeiAbstract:Objective To construct a high efficient UV-attenuated Schistosoma Japonicum cercariae single chain Fv(ScFv)phage display library and screen for the specific ScFv by the potential protective Schistosoma Japonicum cercariae antigen 66-68kDa(SCA66-68kDa),one natural molecular antigen,which based on the high protection of both the UV-attenuated cercariae vaccines and natural molecular vaccine.Methods After selected the optimum conditions of ultraviolet irradiation,the BALB / c mice were immunized with UV-attenuated Schistosoma Japonicum cercariae,then the serum of these mice was transferred passively to other mice.In order to evaluate the protection potential of UV-attenuated cercariae serum,once we get the protective serum,the spleens of these mice was used to prepare RNA.Then we used the methods of phage display antibody library to construct the single-chain Fv(scFv) antibody library against UV-attenuated Schistosoma Japonicum cercariae and characterized it from the way of capacity and diversity.scFv antibody library was screened against UV-attenuated Schistosoma Japonicum cercariae with the purified antigen of
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Combined immunoprotection of Schistosoma Japonicum ribosomal protein S4 gene and recombinant protein
Chinese journal of zoonoses, 2010Co-Authors: Wu Ping, Wang Shiping, Gao Dongmei, Wen Zhili, Yu LuxinAbstract:To study the protective efficacy on co-immunization of Schistosoma Japonicum ribosomal protein S4(SjRPS4)gene and recombinant protein against Schistosoma Japonicum in mice,which all were immunized three times with plasmids pcDNA3.0/SjRPS4,protein of SjRPS4 or both the DNA,and protein boosting vaccination in an interval of 2 weeks.Then they were challenged by 20 cercariae of Schistosoma Japonicum 2 weeks after final immunization.All mice were sacrificed after 45 days postchallenge and the numbers of worms and eggs in livers,intestines,feces and uterus tissues were counted.Compared with the normal saline group,the worm reduction rate and egg reduction rate in feces,uterus,livers,and intestine tissues in DNA priming-protein boosting vaccination group were 49.7%,62.6%,46.0%,49.0% and 54.3%,which were significantly higher than those in SjRPS4 DNA group(the worm reduction rate and egg reduction rate in feces,uterus,liver,and intestine tissues were 36.2%,48.7%,35.2%,41.8% and 40.5%)and in recombinant protein group(the worm reduction rate and egg reduction rate in feces,uterus,liver,and intestine tissues were 38.2%,50.1%,38.9% and 42.4%)(P0.05).Results suggested that SjRPS4 DNA and recombinant protein vaccination could induce partial protective immunity against Schistosoma Japonicum in mice.Meanwhile,the DNA priming-protein boosting vaccination regimen is better than the single vaccination.
Yulan Wang - One of the best experts on this subject based on the ideXlab platform.
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Salmonella typhimurium Infection Reduces Schistosoma Japonicum Worm Burden in Mice.
Scientific reports, 2017Co-Authors: Xiaoyang Zhu, Lu Chen, Huiru Tang, Yulan WangAbstract:Coinfection of microorganisms is a common phenomenon in humans and animals. In order to further our understanding of the progress of coinfection and the possible interaction between different pathogens, we have built a coinfection mouse model with Schistosoma Japonicum and Salmonella typhimurium, and used this model to investigate the systemic metabolic and immune responses using NMR-based metabonomics and immunological techniques. Our results show that Salmonella typhimurium (ATCC14028) infection reduces the number of adult Schistosomal worms and eggs, relieves symptoms of schistosomiasis and also abates the mortality of mice infected by Schistosoma Japonicum. In addition, Salmonella typhimurium infection counteracts the metabolic disturbances associated with schistosomiasis, which was reflected by the reverted levels of metabolites in coinfected mice, compared with the Schistosoma Japonicum infected mice. Furthermore, immune analyses also indicate that shift of the immune response to different pathogens is a result of indirect interactions between Schistosoma Japonicum and Salmonella typhimurium within the host. Salmonella typhimurium infection can ameliorate Schistosoma Japonicum-caused schistosomiasis in BALB/c mice, which is most likely due to inverse immune polarization. Our work provides an insight into coinfection between Schistosoma Japonicum and Salmonella typhimurium, and may further contribute to the development of new tools for controlling Schistosoma Japonicum-associated diseases.
