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Maria Armanda Reis-henriques - One of the best experts on this subject based on the ideXlab platform.
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Organochlorine bioaccumulation and biomarkers levels in culture and wild white Seabream (Diplodus sargus)
Chemosphere, 2008Co-Authors: Marta Ferreira, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Paulo Antunes, Joana Amado, Odete Gil, Maria Armanda Reis-henriquesAbstract:Abstract Persistent organic pollutants (POPs), which can accumulate in the adipose fish tissues, can enter the human food chain through the consumption of fish, and cause risk to health. The use of chemical analysis, and biochemical and cellular responses is a way to detect the impact of pollutants in aquatic systems. The purpose of this study was to investigate the levels of organochlorine compounds (polychlorinated biphenyls – PCB and p,p′-dichlorodiphenyltrichloroethane and its metabolites – tDDT) in, wild and cultivated, white Seabream (Diplodus sargus), and also its biological effects that were evaluated by assessing the activity of biotransformation enzymes and genotoxic effects. To achieve that we have sampled five different size classes (I – 13 g, II – 64 g, III – 143 g, IV – 315 g and V – 441 g) of white Seabream from a local aquaculture, and also a group of wild fish (375 g) in order to compare accumulation and responses between cultured and wild fish. White Seabream, cultured and wild, presented low levels of organochlorine content, both in liver and in muscle. Wild white Seabream, in comparison to cultured ones at the marketable size, showed lower organochlorine accumulation. Biotransformation enzymes showed negative correlations with organochlorine levels in liver. Micronucleous numbers revealed that wild white Seabream are not so exposed to genotoxic compounds as cultured ones.
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Metal accumulation and oxidative stress responses in, cultured and wild, white Seabream from Northwest Atlantic.
The Science of the total environment, 2008Co-Authors: Marta Ferreira, Miguel Caetano, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Maria Armanda Reis-henriquesAbstract:Metals are environmentally ubiquitous and can be found at high concentrations in seawater and subsequently in marine organisms. Metals with high redox potential can trigger oxidative stress mechanisms with damaging effects in biological tissues. In aquatic species, oxidative stress has been evaluated by assessing antioxidant enzymes activities and oxidative damages in tissues. The purpose of this study was to evaluate oxidative stress biomarkers and metal residues in white Seabream (Diplodus sargus), a species entering aquaculture production in Portugal. Metal residues (Cu, Cd, As and Pb), in liver and muscle, as well as oxidative stress biomarkers were assessed at different stages in the life cycle of white Seabream under culture conditions and in wild specimens, of a marketable size. Metal concentrations in tissues were low, and below the established limits. However, wild white Seabream showed higher accumulation than cultured ones. Antioxidant enzymes, namely catalase (CAT) and superoxide dismutase (SOD), were correlated with metal accumulation. Oxidative damages to tissues were low, with wild white Seabream showing lower levels than cultured fish. This study showed that white Seabream has a good antioxidant defense system, capable of reducing oxidative damages in tissues resulting from the presence of metals.
Marta Ferreira - One of the best experts on this subject based on the ideXlab platform.
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Organochlorine bioaccumulation and biomarkers levels in culture and wild white Seabream (Diplodus sargus)
Chemosphere, 2008Co-Authors: Marta Ferreira, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Paulo Antunes, Joana Amado, Odete Gil, Maria Armanda Reis-henriquesAbstract:Abstract Persistent organic pollutants (POPs), which can accumulate in the adipose fish tissues, can enter the human food chain through the consumption of fish, and cause risk to health. The use of chemical analysis, and biochemical and cellular responses is a way to detect the impact of pollutants in aquatic systems. The purpose of this study was to investigate the levels of organochlorine compounds (polychlorinated biphenyls – PCB and p,p′-dichlorodiphenyltrichloroethane and its metabolites – tDDT) in, wild and cultivated, white Seabream (Diplodus sargus), and also its biological effects that were evaluated by assessing the activity of biotransformation enzymes and genotoxic effects. To achieve that we have sampled five different size classes (I – 13 g, II – 64 g, III – 143 g, IV – 315 g and V – 441 g) of white Seabream from a local aquaculture, and also a group of wild fish (375 g) in order to compare accumulation and responses between cultured and wild fish. White Seabream, cultured and wild, presented low levels of organochlorine content, both in liver and in muscle. Wild white Seabream, in comparison to cultured ones at the marketable size, showed lower organochlorine accumulation. Biotransformation enzymes showed negative correlations with organochlorine levels in liver. Micronucleous numbers revealed that wild white Seabream are not so exposed to genotoxic compounds as cultured ones.
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Metal accumulation and oxidative stress responses in, cultured and wild, white Seabream from Northwest Atlantic.
The Science of the total environment, 2008Co-Authors: Marta Ferreira, Miguel Caetano, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Maria Armanda Reis-henriquesAbstract:Metals are environmentally ubiquitous and can be found at high concentrations in seawater and subsequently in marine organisms. Metals with high redox potential can trigger oxidative stress mechanisms with damaging effects in biological tissues. In aquatic species, oxidative stress has been evaluated by assessing antioxidant enzymes activities and oxidative damages in tissues. The purpose of this study was to evaluate oxidative stress biomarkers and metal residues in white Seabream (Diplodus sargus), a species entering aquaculture production in Portugal. Metal residues (Cu, Cd, As and Pb), in liver and muscle, as well as oxidative stress biomarkers were assessed at different stages in the life cycle of white Seabream under culture conditions and in wild specimens, of a marketable size. Metal concentrations in tissues were low, and below the established limits. However, wild white Seabream showed higher accumulation than cultured ones. Antioxidant enzymes, namely catalase (CAT) and superoxide dismutase (SOD), were correlated with metal accumulation. Oxidative damages to tissues were low, with wild white Seabream showing lower levels than cultured fish. This study showed that white Seabream has a good antioxidant defense system, capable of reducing oxidative damages in tissues resulting from the presence of metals.
Jingui Xie - One of the best experts on this subject based on the ideXlab platform.
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Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream (Acanthopagrus Latus).
International journal of molecular sciences, 2020Co-Authors: Genmei Lin, Wenyu Fang, Dong Gao, Jing Huang, Jingui XieAbstract:Yellowfin Seabream (Acanthopagrus latus) is a commercially important fish in Asian coastal waters. Although natural sex reversal has been described in yellowfin Seabream, the mechanisms underlying sexual differentiation and gonadal development in this species remain unclear. MicroRNAs (miRNAs) have been shown to play crucial roles in gametogenesis and gonadal development. Here, two libraries of small RNAs, constructed from the testes and ovaries of yellowfin Seabream, were sequenced. Across both gonads, we identified 324 conserved miRNAs and 92 novel miRNAs: 67 ovary-biased miRNAs, including the miR-200 families, the miR-29 families, miR-21, and miR-725; and 88 testis-biased miRNAs, including the let-7 families, the miR-10 families, miR-7, miR-9, and miR-202-3p. GO (Gene Ontology) annotations and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses of putative target genes indicated that many target genes were significantly enriched in the steroid biosynthesis pathway and in the reproductive process. Our integrated miRNA-mRNA analysis demonstrated a putative negatively correlated expression pattern in yellowfin Seabream gonads. This study profiled the expression patterns of sex-biased miRNAs in yellowfin Seabream gonads, and provided important molecular resources that will help to clarify the miRNA-mediated post-transcriptional regulation of sexual differentiation and gonadal development in this species.
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Gonadal Transcriptome Analysis of Sex-Related Genes in the Protandrous Yellowfin Seabream (Acanthopagrus latus).
Frontiers in genetics, 2020Co-Authors: Genmei Lin, Wenyu Fang, Dong Gao, Jing Huang, Peilin Huang, Jingui XieAbstract:Yellowfin Seabream (Acanthopagrus latus), a protandrous hermaphroditic fish, is a good model for studying the mechanism of sex reversal. However, limited knowledge is known about the genetic information related to reproduction and sex differentiation in this species. Here, we performed de novo transcriptome sequencing analysis of the testis, ovotestis, and ovary to identify sex-related genes in yellowfin Seabream. The results assembled 71,765 unigenes in which 16,126 and 17,560 unigenes were differentially expressed in the ovotestis and ovary compared to the testis, respectively. The most differentially expressed gene (DEG)-enriched Kyoto Encyclopedia of Genes and Genomes and GO pathways were closely associated with the synthesis of sex steroid hormones. Functional analyses identified 55 important sex-related DEGs, including 32 testis-biased DEGs (dmrt1, amh, and sox9, etc.), 20 ovary-biased DEGs (cyp19a, foxl2, and wnt4, etc.), and 3 ovotestis-biased DEGs (lhb, dmrt2, and foxh1). Furthermore, the testis-specific expression of dmrt1 and the brain-pituitary-ovary axis expression of foxl2 were characterized, suggesting that they might play important roles in sex differentiation in yellowfin Seabream. Our present work provided an important molecular basis for elucidating the mechanisms underlying sexual transition and reproductional regulation in yellowfin Seabream.
Joana Costa - One of the best experts on this subject based on the ideXlab platform.
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Organochlorine bioaccumulation and biomarkers levels in culture and wild white Seabream (Diplodus sargus)
Chemosphere, 2008Co-Authors: Marta Ferreira, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Paulo Antunes, Joana Amado, Odete Gil, Maria Armanda Reis-henriquesAbstract:Abstract Persistent organic pollutants (POPs), which can accumulate in the adipose fish tissues, can enter the human food chain through the consumption of fish, and cause risk to health. The use of chemical analysis, and biochemical and cellular responses is a way to detect the impact of pollutants in aquatic systems. The purpose of this study was to investigate the levels of organochlorine compounds (polychlorinated biphenyls – PCB and p,p′-dichlorodiphenyltrichloroethane and its metabolites – tDDT) in, wild and cultivated, white Seabream (Diplodus sargus), and also its biological effects that were evaluated by assessing the activity of biotransformation enzymes and genotoxic effects. To achieve that we have sampled five different size classes (I – 13 g, II – 64 g, III – 143 g, IV – 315 g and V – 441 g) of white Seabream from a local aquaculture, and also a group of wild fish (375 g) in order to compare accumulation and responses between cultured and wild fish. White Seabream, cultured and wild, presented low levels of organochlorine content, both in liver and in muscle. Wild white Seabream, in comparison to cultured ones at the marketable size, showed lower organochlorine accumulation. Biotransformation enzymes showed negative correlations with organochlorine levels in liver. Micronucleous numbers revealed that wild white Seabream are not so exposed to genotoxic compounds as cultured ones.
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Metal accumulation and oxidative stress responses in, cultured and wild, white Seabream from Northwest Atlantic.
The Science of the total environment, 2008Co-Authors: Marta Ferreira, Miguel Caetano, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Maria Armanda Reis-henriquesAbstract:Metals are environmentally ubiquitous and can be found at high concentrations in seawater and subsequently in marine organisms. Metals with high redox potential can trigger oxidative stress mechanisms with damaging effects in biological tissues. In aquatic species, oxidative stress has been evaluated by assessing antioxidant enzymes activities and oxidative damages in tissues. The purpose of this study was to evaluate oxidative stress biomarkers and metal residues in white Seabream (Diplodus sargus), a species entering aquaculture production in Portugal. Metal residues (Cu, Cd, As and Pb), in liver and muscle, as well as oxidative stress biomarkers were assessed at different stages in the life cycle of white Seabream under culture conditions and in wild specimens, of a marketable size. Metal concentrations in tissues were low, and below the established limits. However, wild white Seabream showed higher accumulation than cultured ones. Antioxidant enzymes, namely catalase (CAT) and superoxide dismutase (SOD), were correlated with metal accumulation. Oxidative damages to tissues were low, with wild white Seabream showing lower levels than cultured fish. This study showed that white Seabream has a good antioxidant defense system, capable of reducing oxidative damages in tissues resulting from the presence of metals.
Carlos Vale - One of the best experts on this subject based on the ideXlab platform.
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Organochlorine bioaccumulation and biomarkers levels in culture and wild white Seabream (Diplodus sargus)
Chemosphere, 2008Co-Authors: Marta Ferreira, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Paulo Antunes, Joana Amado, Odete Gil, Maria Armanda Reis-henriquesAbstract:Abstract Persistent organic pollutants (POPs), which can accumulate in the adipose fish tissues, can enter the human food chain through the consumption of fish, and cause risk to health. The use of chemical analysis, and biochemical and cellular responses is a way to detect the impact of pollutants in aquatic systems. The purpose of this study was to investigate the levels of organochlorine compounds (polychlorinated biphenyls – PCB and p,p′-dichlorodiphenyltrichloroethane and its metabolites – tDDT) in, wild and cultivated, white Seabream (Diplodus sargus), and also its biological effects that were evaluated by assessing the activity of biotransformation enzymes and genotoxic effects. To achieve that we have sampled five different size classes (I – 13 g, II – 64 g, III – 143 g, IV – 315 g and V – 441 g) of white Seabream from a local aquaculture, and also a group of wild fish (375 g) in order to compare accumulation and responses between cultured and wild fish. White Seabream, cultured and wild, presented low levels of organochlorine content, both in liver and in muscle. Wild white Seabream, in comparison to cultured ones at the marketable size, showed lower organochlorine accumulation. Biotransformation enzymes showed negative correlations with organochlorine levels in liver. Micronucleous numbers revealed that wild white Seabream are not so exposed to genotoxic compounds as cultured ones.
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Metal accumulation and oxidative stress responses in, cultured and wild, white Seabream from Northwest Atlantic.
The Science of the total environment, 2008Co-Authors: Marta Ferreira, Miguel Caetano, Joana Costa, Pedro Pousão-ferreira, Carlos Vale, Maria Armanda Reis-henriquesAbstract:Metals are environmentally ubiquitous and can be found at high concentrations in seawater and subsequently in marine organisms. Metals with high redox potential can trigger oxidative stress mechanisms with damaging effects in biological tissues. In aquatic species, oxidative stress has been evaluated by assessing antioxidant enzymes activities and oxidative damages in tissues. The purpose of this study was to evaluate oxidative stress biomarkers and metal residues in white Seabream (Diplodus sargus), a species entering aquaculture production in Portugal. Metal residues (Cu, Cd, As and Pb), in liver and muscle, as well as oxidative stress biomarkers were assessed at different stages in the life cycle of white Seabream under culture conditions and in wild specimens, of a marketable size. Metal concentrations in tissues were low, and below the established limits. However, wild white Seabream showed higher accumulation than cultured ones. Antioxidant enzymes, namely catalase (CAT) and superoxide dismutase (SOD), were correlated with metal accumulation. Oxidative damages to tissues were low, with wild white Seabream showing lower levels than cultured fish. This study showed that white Seabream has a good antioxidant defense system, capable of reducing oxidative damages in tissues resulting from the presence of metals.