The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
J. P. Gustafson - One of the best experts on this subject based on the ideXlab platform.
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Dosage response of rye genes in a wheat background
Theoretical and Applied Genetics, 1992Co-Authors: D. C. Bittel, J. P. GustafsonAbstract:A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression. Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
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Dosage response of rye genes in a wheat background : 2. Secalin genes on 1RS.
Theoretical and Applied Genetics, 1992Co-Authors: D. C. Bittel, J. P. GustafsonAbstract:A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression.
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Dosage response of rye genes in a wheat background : 2. Secalin genes on 1RS.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1992Co-Authors: D. C. Bittel, J. P. GustafsonAbstract:A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression.Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
Ji Zeng Jia - One of the best experts on this subject based on the ideXlab platform.
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Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation
Cell research, 2005Co-Authors: Jian Fang Chai, Xu Liu, Ji Zeng JiaAbstract:Wheat 1BL/1RS translocations are widely planted in China as well as in most of the wheat producing area in the world for their good qualities of disease resistance and high yield. 1BL/1RS translocations are however poor in bread making, partially caused by a family of small monomeric proteins, ω-Secalins, which are encoded by genes on 1RS. Based on published sequence of a rye ω-secalin gene we designed a pair of primers to cover the whole mature protein coding sequence. A major band could be amplified from 1BL/1RS translocations but not from euploid wheat. Using this primer set we conducted PCR amplification by using high fidelity Pfu polymerase on the genomic DNAs and cDNAs purified from a 1BL/1RS translocation Lankao 906. Sequencing analysis indicated that this gene family contains several members of 1150 bp, 1076 bp, 1075 bp, 1052 bp and 1004 bp genes, including two pseudogenes and three active genes. The gene transcripts were differentially expressed in developing seeds.
D. C. Bittel - One of the best experts on this subject based on the ideXlab platform.
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Dosage response of rye genes in a wheat background
Theoretical and Applied Genetics, 1992Co-Authors: D. C. Bittel, J. P. GustafsonAbstract:A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression. Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
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Dosage response of rye genes in a wheat background : 2. Secalin genes on 1RS.
Theoretical and Applied Genetics, 1992Co-Authors: D. C. Bittel, J. P. GustafsonAbstract:A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression.
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Dosage response of rye genes in a wheat background : 2. Secalin genes on 1RS.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1992Co-Authors: D. C. Bittel, J. P. GustafsonAbstract:A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression.Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
Jian Fang Chai - One of the best experts on this subject based on the ideXlab platform.
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Molecular identification of ω-secalin gene expression activity in a wheat 1B/1R translocation cultivar
Journal of Integrative Agriculture, 2016Co-Authors: Jian Fang Chai, Cui-mian Zhang, Hai-bo WangAbstract:Abstract ω-Secalin was an important factor influencing processing quality of wheat 1BL/1RS translocations. On the basis of four ω-secalin gene sequences cloned from Lankao 906 (a wheat cultivar with 1BL/1RS translocation) with putative transcription activity, a pair of primers with suitable restriction endonucleases added at their 5′ ends were designed to amplify the mature protein-coding regions of the four genes. After digestion with restriction endonucleases, the amplified products were ligated into the prokaryotic expression vector pET30a(+). The prokaryotically expressed recombinant proteins and gliadin isolated from the Lankao 906 seed were analyzed on the same acid polyacrylamide gel electrophoresis. All four prokaryotically expressed secalin bands had corresponding seed-expressed gliadin bands. The four corresponding gliadin bands were confirmed to be the expression products of the four ω-secalin genes by liquid chromatography tandem mass spectrometry (LC-MS/MS). This conclusion was further confirmed because the four ω-secalin bands could be detected in all 14 wheat 1BL/1RS translocation cultivars used in the study, although there was some interference for the detection of one ω-secalin band from nearby wheat gliadin bands. The sequence information of ω-secalin genes with expression activity will be helpful for improving the processing quality of wheat with 1BL/1RS translocations by using RNA interference method to silence the expression of the ω-secalin genes.
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Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation
Cell research, 2005Co-Authors: Jian Fang Chai, Xu Liu, Ji Zeng JiaAbstract:Wheat 1BL/1RS translocations are widely planted in China as well as in most of the wheat producing area in the world for their good qualities of disease resistance and high yield. 1BL/1RS translocations are however poor in bread making, partially caused by a family of small monomeric proteins, ω-Secalins, which are encoded by genes on 1RS. Based on published sequence of a rye ω-secalin gene we designed a pair of primers to cover the whole mature protein coding sequence. A major band could be amplified from 1BL/1RS translocations but not from euploid wheat. Using this primer set we conducted PCR amplification by using high fidelity Pfu polymerase on the genomic DNAs and cDNAs purified from a 1BL/1RS translocation Lankao 906. Sequencing analysis indicated that this gene family contains several members of 1150 bp, 1076 bp, 1075 bp, 1052 bp and 1004 bp genes, including two pseudogenes and three active genes. The gene transcripts were differentially expressed in developing seeds.
R. Appels - One of the best experts on this subject based on the ideXlab platform.
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A gene from the Sec2 (Gli-R2) locus of a wheat 2RS.2BL chromosomal translocation line
Theoretical and Applied Genetics, 2001Co-Authors: F. R. Murray, J. H. Skerritt, R. AppelsAbstract:A genomic DNA clone coding for a rye secalin gene (gSec2A) was isolated from a wheat translocation line carrying the 2RS.2BL chromosome, using a previously identified partial secalin (Sec2) cDNA clone as a probe. The predicted N-terminal amino-acid sequence of the gSec2A gene was identical to the N-terminal sequence obtained for Sec2 polypeptide bands isolated from SDS-PAGE gels. Bacterially expressed gSec2A protein was identical in size to that of the smallest Sec2 polypeptide band observed on SDS PAGE gels and is recognized by a monoclonal antibody specific for Mr 75000 2RS γ-Secalins. Overall, the predicted protein sequence of gSec2A was most similar (50%) to the family of γ-gliadins and consists of a short N-terminal region containing one cysteine residue followed by a glutamine/proline-rich repetitive domain and a long C-terminal domain containing eight cysteine residues. The repetitive domain can be divided into two regions. One region coded for 15 units, each consisting of eight amino acids similar in sequence to that found in the ω-Secalins and C-hordeins. The second region coded for 17 units each consisting of a sequence of 7–10 amino acids similar to that observed in γ-gliadins.
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Sequence variation at theSec-1 locus of rye,Secale cereale (Poaceae)
Plant Systematics and Evolution, 1999Co-Authors: B C Clarke, R. AppelsAbstract:This paper describes the structure of a 9.2-kb repeat unit of DNA, which represents one ω -secalin gene and spacer sequence located at the Sec -1 locus on the short arm of chromosome 1 of rye. The gene units at the Sec -1 locus comprise 1.1 kb representing the gene and 8.1 kb of spacer sequence separating the genes. A sequence comparison of nine genes and their promoter regions from the Sec -1 locus, reveals that there is greater variation within the coding sequence than there is within the promoter regions. The gene sequence variation is discussed in terms of the size variation seen for the ω -secalin proteins in rye species. The results include a comparison of promoter sequences from members of the Triticeae to examine the degree of conservation between other seed storage protein genes.
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A transient assay for evaluating promoters in wheat endosperm tissue
Genome, 1998Co-Authors: B C Clarke, R. AppelsAbstract:A transient assay was developed for the evaluation of promoter sequences in wheat endosperm tissue. A deletion series from an omega-secalin gene promoter, located on chromosome 1RS.1DL of specific wheat lines, were translationally fused to a uidA reporter gene. These promoters were evaluated for expression in wheat endosperm tissue after integration of the DNA into the cell using microprojectile bombardment. The results were compared with those obtained using other transient assay systems.Key words: particle bombardment, transient assay, omega-secalin gene, wheat endosperm.
