The Experts below are selected from a list of 37041 Experts worldwide ranked by ideXlab platform
Zhonghu He - One of the best experts on this subject based on the ideXlab platform.
-
wheat Seed Storage proteins advances in molecular genetics diversity and breeding applications
Journal of Cereal Science, 2014Co-Authors: Awais Rasheed, R Appels, Tariq Mahmood, Zhonghu HeAbstract:Wheat Seed Storage proteins, especially glutenins and gliadins, have unique functional properties giving rise to a wide array of food products for human consumption. The wheat Seed Storage proteins, however, are also the most common cause of food-related allergies and intolerances, and it has become crucially important to understand their composition, variation and functional properties and interface this knowledge with the grain handling industry as well as the breeders. This review focuses on advances in understanding the genetics and function of Storage proteins and their application in wheat breeding programs. These include: (1) The development and validation of high-throughput molecular marker systems for defining the composition and variation of low molecular weight glutenin subunits (LMW-GS) genes and a summary of the more than 30 gene-specific markers for rapid screening in wheat breeding programs; (2) The identification of more than 100 alleles of Storage proteins in wild species provide candidate genes for future quality improvement; (3) The documentation of quality effects of individual LMW-GS and HMW-GS for improving end-use quality; and (4) The analysis of α-gliadin genes on chromosomes 6A and 6D with non-toxic epitopes as potential targets to develop less toxic cultivars for people with celiac disease. Genomic and proteomic technologies that will continue to provide new tools for understanding variation and function of Seed Storage proteins in wheat are discussed.
Awais Rasheed - One of the best experts on this subject based on the ideXlab platform.
-
wheat Seed Storage proteins advances in molecular genetics diversity and breeding applications
Journal of Cereal Science, 2014Co-Authors: Awais Rasheed, R Appels, Tariq Mahmood, Zhonghu HeAbstract:Wheat Seed Storage proteins, especially glutenins and gliadins, have unique functional properties giving rise to a wide array of food products for human consumption. The wheat Seed Storage proteins, however, are also the most common cause of food-related allergies and intolerances, and it has become crucially important to understand their composition, variation and functional properties and interface this knowledge with the grain handling industry as well as the breeders. This review focuses on advances in understanding the genetics and function of Storage proteins and their application in wheat breeding programs. These include: (1) The development and validation of high-throughput molecular marker systems for defining the composition and variation of low molecular weight glutenin subunits (LMW-GS) genes and a summary of the more than 30 gene-specific markers for rapid screening in wheat breeding programs; (2) The identification of more than 100 alleles of Storage proteins in wild species provide candidate genes for future quality improvement; (3) The documentation of quality effects of individual LMW-GS and HMW-GS for improving end-use quality; and (4) The analysis of α-gliadin genes on chromosomes 6A and 6D with non-toxic epitopes as potential targets to develop less toxic cultivars for people with celiac disease. Genomic and proteomic technologies that will continue to provide new tools for understanding variation and function of Seed Storage proteins in wheat are discussed.
Mingxun Chen - One of the best experts on this subject based on the ideXlab platform.
-
MYC2, MYC3, and MYC4 function redundantly in Seed Storage protein accumulation in Arabidopsis.
Plant physiology and biochemistry : PPB, 2016Co-Authors: Chenhao Gao, Kaige Liu, Changyu Jin, Gengqing Huang, Jiangbo Hai, Meng Zhang, Mingxun ChenAbstract:Basic helix-loop-helix transcription factors (TFs), namely MYC2, MYC3, and MYC4, interact with Jasmonate Zim-domain proteins and are their direct targets. These TFs have been shown to function synergistically to control Arabidopsis growth and development. Our results showed similar MYC2, MYC3, and MYC4 expression patterns during Arabidopsis Seed development, which remained relatively high during Seed mid-maturation. MYC2, MYC3, and MYC4 acted redundantly in Seed size, weight control, and in regulating Seed Storage protein accumulation. Triple mutants produced the largest Seeds and single and double mutants' Seeds were much larger than those of wild type. The weight of triple mutants' Seeds was significantly higher than that of wild-type Seeds, which was accompanied by an increase in Seed Storage protein contents. Triple mutants' Seeds presented a marked decrease in 2S amounts relative to those in wild-type Seeds. Liquid chromatography tandem mass spectra sequencing results indicated that both the relative abundance and the peptide number of CRA1 and CRU3 were greatly increased in triple mutants compared to wild type. The expression of 2S1-2S5 decreased and that of CRA1 and CRU3 increased in triple mutants relative to those in wild types during Seed development, which might have contributed to the low 2S and high 12S contents in triple mutants. Our results contribute to understanding the function of MYC2, MYC3, and MYC4 on Seed development, and provide promising targets for genetic manipulations of protein-producing crops to improve the quantity and quality of Seed Storage proteins.
-
TRANSPARENT TESTA GLABRA1 Regulates the Accumulation of Seed Storage Reserves in Arabidopsis
Plant physiology, 2015Co-Authors: Mingxun Chen, Bin Zhang, Harikrishna Kulaveerasingam, Fook Tim ChewAbstract:Seed Storage reserves mainly consist of starch, triacylglycerols, and Storage proteins. They not only provide energy for Seed germination and Seedling establishment, but also supply essential dietary nutrients for human beings and animals. So far, the regulatory networks that govern the accumulation of Seed Storage reserves in plants are still largely unknown. Here, we show that TRANSPARENT TESTA GLABRA1 (TTG1), which encodes a WD40 repeat transcription factor involved in many aspects of plant development, plays an important role in mediating the accumulation of Seed Storage reserves in Arabidopsis (Arabidopsis thaliana). The dry weight of ttg1-1 embryos significantly increases compared with that of wild-type embryos, which is accompanied by an increase in the contents of starch, total protein, and fatty acids in ttg1-1 Seeds. FUSCA3 (FUS3), a master regulator of Seed maturation, binds directly to the TTG1 genomic region and suppresses TTG1 expression in developing Seeds. TTG1 negatively regulates the accumulation of Seed Storage proteins partially through transcriptional repression of 2S3, a gene encoding a 2S albumin precursor. TTG1 also indirectly suppresses the expression of genes involved in either Seed development or synthesis/modification of fatty acids in developing Seeds. In addition, we demonstrate that the maternal allele of the TTG1 gene suppresses the accumulation of Storage proteins and fatty acids in Seeds. Our results suggest that TTG1 is a direct target of FUS3 in the framework of the regulatory hierarchy controlling Seed filling and regulates the accumulation of Seed Storage proteins and fatty acids during the Seed maturation process.
C.a Smith - One of the best experts on this subject based on the ideXlab platform.
-
Kenaf Seed Storage duration on germination, emergence, and yield ☆
Industrial Crops and Products, 2003Co-Authors: Paul D. Meints, C.a SmithAbstract:Abstract Kenaf ( Hibiscus cannabinus L.) is a potential alternative crop being developed for fiber production. Because planting area varies dramatically from year to year, Seed supplies may greatly exceed use so that the excess Seed must be stored for one to several years. The objectives of this study were to determine the effect of Seed Storage duration at 10 °C on germination, vigor, emergence, and yield. Replicated trials were established at Starkville, MS in 1999 and 2000 to evaluate field emergence and biomass yield of kenaf Seed from five ‘Everglades 41’ (‘E41’) harvest year Seed lots stored at 10 °C in ambient relative humidity for up to 4 years. Germination of these same Seed lots under standard (20–30 °C) and cool (20 °C) temperatures, and Seed vigor was evaluated over time. Field emergence was the same for the different Seed Storage durations up to 4 years, but was directly affected by drought conditions for each planted year. Biomass yields ranged from 12.39 to 14.57 Mg ha −1 in 1999 and 16.82 to 18.47 Mg ha −1 in 2000, but were not different between Storage durations. Seed germination remained greater than 80% regardless of Storage duration. Electrolyte leakage, based on conductivity, was 38–50% less with freshly harvested Seed than Seed stored for 4 years at 10 °C. However, neither the conductivity nor accelerated aging test were reliable predictors of field emergence. Kenaf Seed stored up to 4 years at 10 °C retained germination rates acceptable for commercial use. Neither field emergence nor biomass yield was affected by Seed Storage duration.
Jalaluddin Khan - One of the best experts on this subject based on the ideXlab platform.
-
Phylogeny of ten species of the genus Hordeum L. as revealed by AFLP markers and Seed Storage protein electrophoresis.
Molecular biology reports, 2013Co-Authors: Haddad A. El Rabey, Abdulrahman L. Al-malki, Khalid O. Abulnaja, Mohsen K. H. Ebrahim, Taha A. Kumosani, Jalaluddin KhanAbstract:The phylogenetic relationships of 60 accessions representing ten species of the genus Hordeum were investigated based on AFLP markers and Seed Storage protein SDS-PAGE electrophoresis. A total of 339 AFLP polymorphic markers were scored as a result of fingerprinting the studied taxa using seven AFLP primer combinations, whereas 46 polymorphic protein bands resulted from the water soluble and water non-soluble Seed Storage protein electrophoresis. The phylogenetic tree deduced from AFLP analysis is concordant in a large extent with that deduced from Seed Storage protein electrophoresis. The studied taxa were clustered according to their genome type into two main groups representing the Old and New World’s species. Inside each group the species were clustered according to their genome type. Highly significant cophenetic correlation coefficient was obtained between both AFLP (0.96) and Seed Storage protein (0.89) indicating the reliability of the results.
-
Short communication. Efficiency of AFLP markers and Seed Storage protein electrophoresis to study the phylogeny of some Hordeum species
Spanish Journal of Agricultural Research, 2013Co-Authors: Haddad A. El Rabey, Abdulrahman L. Al-malki, Khalid O. Abulnaja, Taha A. Kumosani, Jalaluddin KhanAbstract:This study is focused on reconstructing the phylogeny of 60 accessions representing ten species of the genus Hordeum based on amplified fragment length polymorphism (AFLP) markers and SDS-PAGE of Seed Storage proteins. We obtained 339 polymorphic AFLP bands and 46 polymorphic protein bands from the SDS-PAGE of water soluble and non-soluble Seed Storage proteins. The phylogenetic tree deduced from AFLP analysis is concordant with that deduced from Seed Storage proteins electrophoresis. The studied taxa were clustered according to their genome type into two main groups representing the Old and New World’s species. Inside each group the species were clustered according to their genome type. Highly significant cophenetic correlation coefficients obtained in both AFLPs (0.96) and Seed Storage proteins (0.89) indicate the reliability of the results. It can be concluded that both AFLP and SDS-PAGE are adequate techniques to study the Hordeum phylogeny.
