Seed Extracts

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A. Mohankumar - One of the best experts on this subject based on the ideXlab platform.

E. S. Karthy - One of the best experts on this subject based on the ideXlab platform.

Kwang-soon Shin - One of the best experts on this subject based on the ideXlab platform.

  • Bioactivity of Trifoliate Orange (Poncirus trifoliate) Seed Extracts
    2016
    Co-Authors: Seong Yeong Kim, Kwang-soon Shin
    Abstract:

    Trifoliate orange Seed Extracts (TSEs) were made using either distilled water (TW), ethanol (TE), or n-hexane (TH), to measure total polyphenol contents, DPPH and ABTS radical scavenging activities, and anti-complementary activity. The results showed that the total polyphenol content showed higher value at TE (235.24 μg/mL, p 0.05) than those of TW (132.65 μg/mL) and TH (165.44 μg/mL) at 10 mg/mL and TE exerted the highest DPPH radical scavenging activity (61.77%, p 0.05), which occurred in the following order: TE TW (56.87%) TH (39.78%). The results of ABTS radical scavenging activity showed that TW (34.26%) and TE (31.81%) showed similar activities, which were higher than TH (12.74%, p 0.05). Anti-complementary activity of TE (61 % at 500 μg/mL) showed a higher activity when compared with the positive control (60 % at 1,000 μg/mL) polysaccharide-K (PSK), a known immuno-active polysaccharide from Coriolus versicolor. Consequently, among TSEs, TE is a byproduct from trifoliate orange and could be an important source of dietary polyphenolic antioxidant compounds and im-munopotentiating activity, including complement activation. Key words: trifoliate orange Seed extract, polyphenol, antioxidant activity, DPPH radical scavenging activity, anti-complementary activit

  • antimicrobial activity of trifoliate orange poncirus trifoliate Seed Extracts on gram negative food borne pathogens
    Journal of Food Science and Nutrition, 2012
    Co-Authors: Seong Yeong Kim, Kwang-soon Shin
    Abstract:

    Trifoliate orange Seed Extracts (TSEs) were prepared from different solvents, water (TW), ethanol (TE), and n-hexane (TH), and assessed for their antimicrobial activities against six gram-negative food-borne pathogens (Escherichia coli KCTC 1039, Escherichia coli O157:H7 ATCC 43895, Salmonella Enteritidis ATCC 3311, Salmonella Typhimurium KCCM 11862, Shigella sonnei KCTC 2518, and Vibrio parahaemolyticus ATCC 17802). Among the tested TSEs, TE and TH showed a slight inhibition activity on V. parahaemolyticus ATCC 17802, but a good growth inhibition activity on Sal. Typhimurium KCCM 11862. TH and TE showed steady growth inhibition activity with increasing growth time after 6 hr when compared to the control (p<0.05). From these results, we confirmed the possibility of TH and TE as antimicrobial materials.

  • Bioactivity of Trifoliate Orange (Poncirus trifoliate) Seed Extracts
    Preventive nutrition and food science, 2012
    Co-Authors: Seong Yeong Kim, Kwang-soon Shin
    Abstract:

    Trifoliate orange Seed Extracts (TSEs) were made using either distilled water (TW), ethanol (TE), or n-hexane (TH), to measure total polyphenol contents, DPPH and ABTS radical scavenging activities, and anti-complementary activity. The results showed that the total polyphenol content showed higher value at TE (235.24 μg/mL, p 0.05) than those of TW (132.65 μg/mL) and TH (165.44 μg/mL) at 10 mg/mL and TE exerted the highest DPPH radical scavenging activity (61.77%, p 0.05), which occurred in the following order: TE TW (56.87%) TH (39.78%). The results of ABTS radical scavenging activity showed that TW (34.26%) and TE (31.81%) showed similar activities, which were higher than TH (12.74%, p 0.05). Anti-complementary activity of TE (61% at 500 μg/mL) showed a higher activity when compared with the positive control (60% at 1,000 μg/mL) polysaccharide-K (PSK), a known immuno-active polysaccharide from Coriolus versicolor. Consequently, among TSEs, TE is a byproduct from trifoliate orange and could be an important source of dietary polyphenolic antioxidant compounds and immunopotentiating activity, including complement activation.

Renan Campos Chiste - One of the best experts on this subject based on the ideXlab platform.

  • bioactive compounds and scavenging capacity of pulp peel and Seed Extracts of the amazonian fruit quararibea cordata against ros and rns
    Food Research International, 2015
    Co-Authors: Alessandra Berto, Alessandra Braga Ribeiro, Nilson Evelazio De Souza, Eduarda Fernandes, Renan Campos Chiste
    Abstract:

    Abstract Quararibea cordata , an unexploited Amazonian fruit was investigated regarding the identification of phenolic compounds and the scavenging capacity of its pulp, peel and Seed Extracts against reactive oxygen (ROS) and nitrogen species (RNS). The major phenolic compound in the pulp extract was epicatechin (320 μg/g extract), while A-type proanthocyanidin dimer was the major one in the peel and Seed Extracts. Regarding the carotenoid composition, all- trans -zeaxanthin (2.5–42 μg/g extract), all- trans -lutein and all- trans -β-carotene were the major compounds in all Extracts. The Seed extract, which presented the highest content of phenolic compounds and carotenoids, was the most effective scavenger against all RNS (IC 50 from 19.5 to 37.6 μg/mL), while the peel extract presented the highest efficiency against ROS, especially HOCl. Thus, Q. cordata fruit may present an excellent potential to be used as a bioactive compound source with high antioxidant capacity.

  • in vitro scavenging capacity of annatto Seed Extracts against reactive oxygen and nitrogen species
    Food Chemistry, 2011
    Co-Authors: Renan Campos Chiste, Eduarda Fernandes, Adriana Zerlotti Mercadante, Ana Gomes, Jose Lima, Neura Bragagnolo
    Abstract:

    Abstract Bixa orellana L. (annatto), from Bixaceae family, is a native plant of tropical America, which accumulates several carotenoids (including bixin and norbixin), terpenoids, tocotrienols and flavonoids with potential antioxidant activity. In the present study, the in vitro scavenging capacity of annatto Seed Extracts against reactive oxygen species (ROS) and reactive nitrogen species (RNS) was evaluated and compared to the bixin standard. Annatto Extracts were obtained using solvents with different polarities and their phenolic compounds and bixin levels were determined by high performance liquid chromatography coupled to diode array detector. All annatto Extracts were able to scavenge all the reactive species tested at the low μg/mL range, with the exception of superoxide radical. The ethanol:ethyl acetate and ethyl acetate Extracts of annatto Seeds, which presented the highest levels of hypolaetin and bixin, respectively, were the Extracts with the highest antioxidant capacity, although bixin standard presented the lowest IC 50 values.

K K Sakariah - One of the best experts on this subject based on the ideXlab platform.

  • antibacterial and antioxidant activities of grape vitis vinifera Seed Extracts
    Food Research International, 2003
    Co-Authors: G K Jayaprakasha, Tamil Selvi, K K Sakariah
    Abstract:

    Abstract Grape Seeds were powdered and the fatty material was extracted in a Soxhlet extractor with petroleum ether (60–80 °C) for 6 h. The defatted powder was extracted with acetone:water:acetic acid (90:9.5:0.5) and methanol:water:acetic acid (90:9.5:0.5) for 8 h each separately. The Extracts were concentrated under vacuum to obtain crude Extracts, which were analyzed by high performance liquid chromatography with UV detection at 280 nm. Monomeric procyanidin was found to be the major compound being at 48 and 40% in acetone:water:acetic acid (90:9.5:0.5) and methanol:water:acetic acid (90:9.5:0.5) Extracts, respectively. These Extracts were tested for antibacterial activity by pour plate method against Bacillus cereus, Bacillus coagulans, Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. It was found that, Gram-positive bacteria were completely inhibited at 850–1000 ppm, while Gram-negative bacteria were inhibited at 1250–1500 ppm concentration. Radical-scavenging activity of grape Seed Extracts of acetone:water:acetic acid (90:9.5:0.5) and methanol:water:acetic acid (90:9.5:0.5) were compared with BHA at 25 and 50 ppm concentrations by HPLC method using 1, 1-diphenyl-2-picrylhydrazyl (DPPH). The antioxidant capacities of grape Seed Extracts were determined by the formation of phosphomolybdenum complex method. It was found that acetone:water:acetic acid (90:9.5:0.5) extract was better radical scavenger than methanol:water:acetic acid (90:9.5:0.5) extract.