The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Jin-zhi Zhang - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome-wide identification and functional prediction of novel and flowering-related circular RNAs from Trifoliate Orange (Poncirus trifoliata L. Raf.).
Planta, 2018Co-Authors: Ren-fang Zeng, Jing-jing Zhou, Jin-zhi ZhangAbstract:Main conclusion A total of 558 potential circular RNAs (circRNAs) were identified in citrus, and these were analyzed and compared. One hundred seventy-six differentially expressed circRNAs were identified in two genotypes of Trifoliate Orange. Circular RNAs (circRNAs) play diverse roles in transcriptional control and microRNA (miRNA) function. However, little information is known about circRNAs in citrus. To identify citrus circRNAs and investigate their functional roles, high-throughput sequencing of precocious Trifoliate Orange (an early-flowering Trifoliate Orange mutant, Poncirus trifoliata L. Raf.) and its wild type was performed. A total of 558 potential circRNAs were identified by bioinformatic analysis, and 86.02% of these were sense-overlapping circRNAs. Their sequence features, alternative circularization, and other characteristics were investigated in this study. Compared with the wild type, 176 circRNAs were identified as differentially expressed circRNAs, 61 were significantly up-regulated and 115 were down-regulated in precocious Trifoliate Orange, indicating that they may play an important role in the early flowering process. Alternative circularization and differential expression of some circRNAs were verified by Sanger sequencing and real-time polymerase chain reaction. The functions of differentially expressed circRNAs and their host genes were predicted by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. We found that many differentially expressed circRNAs had abundant miRNA binding sites: 29 circRNAs were found to act as the 16 miRNA targets. Overall, these results will help to reveal the biological functions of circRNAs in growth and development of citrus.
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Genome-wide screening and characterization of long non-coding RNAs involved in flowering development of Trifoliate Orange (Poncirus trifoliata L. Raf.).
Scientific reports, 2017Co-Authors: Chen-yang Wang, Sheng-rui Liu, Xiao-yu Zhang, Jin-zhi ZhangAbstract:Long non-coding RNAs (lncRNAs) have been demonstrated to play critical regulatory roles in post-transcriptional and transcriptional regulation in Arabidopsis. However, lncRNAs and their functional roles remain poorly characterized in woody plants, including citrus. To identify lncRNAs and investigate their role in citrus flowering, paired-end strand-specific RNA sequencing was performed for precocious Trifoliate Orange and its wild-type counterpart. A total of 6,584 potential lncRNAs were identified, 51.6% of which were from intergenic regions. Additionally, 555 lncRNAs were significantly up-regulated and 276 lncRNAs were down-regulated in precocious Trifoliate Orange, indicating that lncRNAs could be involved in the regulation of Trifoliate Orange flowering. Comparisons between lncRNAs and coding genes indicated that lncRNAs tend to have shorter transcripts and lower expression levels and that they display significant expression specificity. More importantly, 59 and 7 lncRNAs were identified as putative targets and target mimics of citrus miRNAs, respectively. In addition, the targets of Pt-miR156 and Pt-miR396 were confirmed using the regional amplification reverse-transcription polymerase chain reaction method. Furthermore, overexpression of Pt-miR156a1 and Pt-miR156a1 in Arabidopsis resulted in an extended juvenile phase, short siliques, and smaller leaves in transgenic plants compared with control plants. These findings provide important insight regarding citrus lncRNAs, thus enabling in-depth functional analyses.
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PtFCA from precocious Trifoliate Orange is regulated by alternative splicing and affects flowering time and root development in transgenic Arabidopsis
Tree Genetics & Genomes, 2016Co-Authors: Jin-zhi Zhang, Tian-jia LiuAbstract:The transition to flowering is a major developmental switch in flowering plants. The nuclear RNA-binding protein FCA responds to seasonal signals and abscisic acid (ABA), which can control the flowering time via ambient temperature and autonomous pathways. Citrus FCA ortholog (PtFCA) has been isolated and characterized from precocious Trifoliate Orange (Poncirus trifoliata L. Raf). Three alternatively spliced transcripts of PtFCA (PtFCA1, PtFCA2, and PtFCA3) were isolated. The expression pattern of PtFCA indicated that it may be involved in phase transition in precocious Trifoliate Orange. A functional complementation experiment of PtFCA indicated that PtFCA1 partially rescued the late-flowering phenotype of the fca-1 mutant. There was no influence on flowering time of transgenic Arabidopsis by PtFCA3 as compared with PtFCA2, which exhibits delayed flowering time in a fca-1 background. Meanwhile, these three transcripts also showed different abilities to regulate root development in the fca-1 background. The study of protein–protein interactions suggested that PtFCA may form higher order complexes with PtFY and PtNF-YA7 to regulate timing of the transition from the vegetative to reproductive phase in precocious Trifoliate Orange. ABA and ambient temperature treatments changed the expression of PtFCA and interaction protein. These findings reveal that PtFCA may play important roles in flowering time and root development of precocious Trifoliate Orange through the formation of multiple protein complexes.
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Characterization and Expression Analysis of PtAGL24, a SHORT VEGETATIVE PHASE/AGAMOUS-LIKE 24 (SVP/AGL24)-Type MADS-Box Gene from Trifoliate Orange (Poncirus trifoliata L. Raf.)
Frontiers in plant science, 2016Co-Authors: Lei-ming Sun, Jin-zhi ZhangAbstract:The transition from vegetative to reproductive growth in perennial woody plants does not occur until after several years of repeated seasonal changes and alternative growth. To better understand the molecular basis of flowering regulation in citrus, a MADS-box gene was isolated from Trifoliate Orange (precocious Trifoliate Orange, Poncirus trifoliata L. Raf.). Sequence alignment and phylogenetic analysis showed that the MADS-box gene is more closely related to the homologs of the AGAMOUS-LIKE 24 (AGL24) lineage than to any of the other MADS-box lineages known from Arabidopsis; it is named PtAGL24. Expression analysis indicated that PtAGL24 was widely expressed in the most organs of Trifoliate Orange, with the higher expression in mature flowers discovered by real-time PCR. Ectopic expression of PtAGL24 in wild-type Arabidopsis promoted early flowering and caused morphological changes in class I transgenic Arabidopsis. Yeast two-hybrid assay revealed that PtAGL24 interacted with Arabidopsis AtAGL24 and other partners of AtAGL24, suggesting that the abnormal morphology of PtAGL24 overexpression in transgenic Arabidopsis was likely due to the inappropriate interactions between exogenous and endogenous proteins. Also, PtAGL24 interacted with SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (PtSOC1) and APETALA1 (PtAP1) of citrus. These results suggest that PtAGL24 may play an important role in the process of floral transition but may have diverse functions in citrus development.
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characterization and expression analysis of ptagl24 a short vegetative phase agamous like 24 svp agl24 type mads box gene from Trifoliate Orange poncirus trifoliata l raf
Frontiers in Plant Science, 2016Co-Authors: Lei-ming Sun, Jin-zhi ZhangAbstract:The transition from vegetative to reproductive growth in perennial woody plants does not occur until after several years of repeated seasonal changes and alternative growth. To better understand the molecular basis of flowering regulation in citrus, a MADS-box gene was isolated from Trifoliate Orange (precocious Trifoliate Orange, Poncirus trifoliata L. Raf.). Sequence alignment and phylogenetic analysis showed that the MADS-box gene is more closely related to the homologs of the AGAMOUS-LIKE 24 (AGL24) lineage than to any of the other MADS-box lineages known from Arabidopsis; it is named PtAGL24. Expression analysis indicated that PtAGL24 was widely expressed in the most organs of Trifoliate Orange, with the higher expression in mature flowers discovered by real-time PCR. Ectopic expression of PtAGL24 in wild-type Arabidopsis promoted early flowering and caused morphological changes in class I transgenic Arabidopsis. Yeast two-hybrid assay revealed that PtAGL24 interacted with Arabidopsis AtAGL24 and other partners of AtAGL24, suggesting that the abnormal morphology of PtAGL24 overexpression in transgenic Arabidopsis was likely due to the inappropriate interactions between exogenous and endogenous proteins. Also, PtAGL24 interacted with SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (PtSOC1) and APETALA1 (PtAP1) of citrus. These results suggest that PtAGL24 may play an important role in the process of floral transition but may have diverse functions in citrus development.
Lei-ming Sun - One of the best experts on this subject based on the ideXlab platform.
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Characterization and Expression Analysis of PtAGL24, a SHORT VEGETATIVE PHASE/AGAMOUS-LIKE 24 (SVP/AGL24)-Type MADS-Box Gene from Trifoliate Orange (Poncirus trifoliata L. Raf.)
Frontiers in plant science, 2016Co-Authors: Lei-ming Sun, Jin-zhi ZhangAbstract:The transition from vegetative to reproductive growth in perennial woody plants does not occur until after several years of repeated seasonal changes and alternative growth. To better understand the molecular basis of flowering regulation in citrus, a MADS-box gene was isolated from Trifoliate Orange (precocious Trifoliate Orange, Poncirus trifoliata L. Raf.). Sequence alignment and phylogenetic analysis showed that the MADS-box gene is more closely related to the homologs of the AGAMOUS-LIKE 24 (AGL24) lineage than to any of the other MADS-box lineages known from Arabidopsis; it is named PtAGL24. Expression analysis indicated that PtAGL24 was widely expressed in the most organs of Trifoliate Orange, with the higher expression in mature flowers discovered by real-time PCR. Ectopic expression of PtAGL24 in wild-type Arabidopsis promoted early flowering and caused morphological changes in class I transgenic Arabidopsis. Yeast two-hybrid assay revealed that PtAGL24 interacted with Arabidopsis AtAGL24 and other partners of AtAGL24, suggesting that the abnormal morphology of PtAGL24 overexpression in transgenic Arabidopsis was likely due to the inappropriate interactions between exogenous and endogenous proteins. Also, PtAGL24 interacted with SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (PtSOC1) and APETALA1 (PtAP1) of citrus. These results suggest that PtAGL24 may play an important role in the process of floral transition but may have diverse functions in citrus development.
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characterization and expression analysis of ptagl24 a short vegetative phase agamous like 24 svp agl24 type mads box gene from Trifoliate Orange poncirus trifoliata l raf
Frontiers in Plant Science, 2016Co-Authors: Lei-ming Sun, Jin-zhi ZhangAbstract:The transition from vegetative to reproductive growth in perennial woody plants does not occur until after several years of repeated seasonal changes and alternative growth. To better understand the molecular basis of flowering regulation in citrus, a MADS-box gene was isolated from Trifoliate Orange (precocious Trifoliate Orange, Poncirus trifoliata L. Raf.). Sequence alignment and phylogenetic analysis showed that the MADS-box gene is more closely related to the homologs of the AGAMOUS-LIKE 24 (AGL24) lineage than to any of the other MADS-box lineages known from Arabidopsis; it is named PtAGL24. Expression analysis indicated that PtAGL24 was widely expressed in the most organs of Trifoliate Orange, with the higher expression in mature flowers discovered by real-time PCR. Ectopic expression of PtAGL24 in wild-type Arabidopsis promoted early flowering and caused morphological changes in class I transgenic Arabidopsis. Yeast two-hybrid assay revealed that PtAGL24 interacted with Arabidopsis AtAGL24 and other partners of AtAGL24, suggesting that the abnormal morphology of PtAGL24 overexpression in transgenic Arabidopsis was likely due to the inappropriate interactions between exogenous and endogenous proteins. Also, PtAGL24 interacted with SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (PtSOC1) and APETALA1 (PtAP1) of citrus. These results suggest that PtAGL24 may play an important role in the process of floral transition but may have diverse functions in citrus development.
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Molecular cloning, promoter analysis and functional characterization of APETALA 1-like gene from precocious Trifoliate Orange (Poncirus trifoliata L. Raf.)
Scientia Horticulturae, 2014Co-Authors: Lei-ming Sun, Jin-zhi Zhang, Li MeiAbstract:Abstract For flowering plants, flowering transition is induced in response to endogenous and extrinsic environmental signals. During this process, the members of the APETALA family from MADS-box transcription factors play an important role. Here, to better understand the molecular mechanism of APETALA1 ( AP1 ) in the regulation of citrus flowering, an APETALA1-like homologue gene ( PtAP1 ) was isolated from an early flowering Trifoliate Orange (precocious Trifoliate Orange, Poncirus trifoliata L. Raf) and normal Trifoliate Orange. The results indicated that the sequence of PtAP1 was not any differences in two genotypes of Trifoliate Orange. Sequence alignment and phylogenetic analysis revealed that the PtAP1 fell in the euAP1 clade. Specific spatial and/or temporal patterns of PtAP1 were investigated in precocious Trifoliate Orange by real-time PCR. The results showed that PtAP1 was detected in almost all reproductive tissues, but only faint expression in vegetative tissues. Moreover, the expression pattern of PtAP1 was closely correlated with floral induction and flowering at phase change stage. Transcription of PtAP1 in Arabidopsis ap1-1 mutant showed partially rescued AP1 function. Over-expression of PtAP1 in wild-type Arabidopsis resulted in early flowering and changed architecture. About 1KB promoter sequence of PtAP1 was obtained by chromosome walking from two genotypes of Trifoliate Orange, there was also not nucleotide difference in the two-promoter sequence. Meanwhile, a number of putative cis -regulatory elements were predicted in PtAP1 promoter by bioinformatics analysis. To determine the expression of PtAP1 promoter, GUS reporter gene was transformed into wild-type Arabidopsis under the control of PtAP1 promoter. The results revealed that the PtAP1 promoter had strong activity and showed developmental phase and tissue specificity, which might be ideal for citrus genetic improvement. In addition, a yeast one-hybrid assay indicated that PtAP1 may be interacted with some flowering proteins, which have not yet been functionally characterized in citrus. These findings suggested that PtAP1 may play an important role during the early flowering of precocious Trifoliate Orange.
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Molecular cloning and functional characterization of genes associated with flowering in citrus using an early-flowering Trifoliate Orange (Poncirus trifoliata L. Raf.) mutant.
Plant molecular biology, 2011Co-Authors: Jin-zhi Zhang, Wen-wu Guo, Lei-ming Sun, Dong-liang Zhang, Xiuxin DengAbstract:To isolate differentially expressed genes during the juvenile-to-adult phase transition of an early-flowering Trifoliate Orange mutant (precocious Trifoliate Orange, Poncirus trifoliata), suppression subtractive hybridization was performed. In total, 463 cDNA clones chosen by differential screening of 1,920 clones were sequenced and 178 differentially expressed genes were identified, among which 41 sequences did not match any known nucleotide sequence. Analysis of expression profiles of the differentially expressed genes through hybridization on customized chips revealed their expression change was associated with the phase transition from juvenile to adult in the mutant. Open reading frames of nine selected genes were successfully determined by rapid amplification of cDNA ends. Expression analysis of these genes by real-time RT-PCR showed that transcript levels of several genes were associated with floral induction and inflorescence development. Among these genes, HM596718, a sequence sharing a high degree of similarity with Arabidopsis EARLY FLOWERING 5 (AtELF5) was discovered. Real-time PCR and in situ hybridization indicated its expression pattern was closely correlated with floral induction and flowering of the mutant. Ectopic expression of the gene in Arabidopsis caused early flowering; however, its functional characterization is different than the role of AtELF5 observed in Arabidopsis. A yeast two-hybrid assay indicated that PtELF5 significantly interacted with DUF1336 domain of a hypothetical protein, which has not yet been functionally characterized in woody plants. These findings suggest that PtELF5 may be a novel gene that plays an important role during the early flowering of precocious Trifoliate Orange.
Ying-ning Zou - One of the best experts on this subject based on the ideXlab platform.
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mycorrhizal response strategies of Trifoliate Orange under well watered salt stress and waterlogging stress by regulating leaf aquaporin expression
Plant Physiology and Biochemistry, 2021Co-Authors: Xiaofen Cheng, Ying-ning Zou, Kamil KucaAbstract:Aquaporins (AQPs) involved in water and small molecule transport respond to environmental stress, while it is not clear how arbuscular mycorrhizal fungi (AMF) regulate AQP expression. Here, we investigated the change in leaf water potential and expression level of four tonoplast intrinsic proteins (TIPs), six plasma membrane intrinsic proteins (PIPs), and four nodin-26 like intrinsic proteins (NIPs) genes in Trifoliate Orange (Poncirus trifoliata) inoculated with Funneliformis mosseae under well-watered (WW), salt stress (SS), and waterlogging stress (WS). Root AMF colonization and soil hyphal length collectively were reduced by SS and WS. Under WW, inoculation with AMF gave diverse responses of AQPs: six AQPs up-regulated, three AQPs down-regulated, and five AQPs did not change. Such up-regulation of more AQPs under mycorrhization and WW partly accelerated water absorption, thereby, maintaining higher leaf water potential. However, under SS, all the fourteen AQPs were dramatically induced by AMF inoculation, which improved water permeability of membranes and stimulated water transport of the host. Under WS, AMF colonization almost did not induce or even down-regulated these AQPs expressions with three exceptions (PtTIP2;2, PtPIP1;1, and PtNIP1;2), thus, no change in leaf water potential. As a result, mycorrhizal plants under flooding may have an escape mechanism to reduce water absorption. It is concluded that AMF had different strategies in response to environmental stresses (e.g. SS and WS) by regulating leaf AQP expression in the host (e.g. Trifoliate Orange).
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Arbuscular Mycorrhizal Fungi Regulate Polyamine Homeostasis in Roots of Trifoliate Orange for Improved Adaptation to Soil Moisture Deficit Stress.
Frontiers in plant science, 2021Co-Authors: Ying-ning Zou, Fei Zhang, A. K. Srivastava, Kamil KucaAbstract:Soil arbuscular mycorrhizal fungi (AMF) enhance the tolerance of plants against soil moisture deficit stress (SMDS), but the underlying mechanisms are still not fully understood. Polyamines (PAs) as low-molecular-weight, aliphatic polycations have strong roles in abiotic stress tolerance of plants. We aimed to investigate the effect of AMF (Funneliformis mosseae) inoculation on PAs, PA precursors, activities of PA synthases and degrading enzymes, and concentration of reactive oxygen species in the roots of Trifoliate Orange (Poncirus trifoliata) subjected to 15 days of SMDS. Leaf water potential and total chlorophyll levels were comparatively higher in AMF-inoculated than in non-AMF-treated plants exposed to SMDS. Mycorrhizal plants recorded a significantly higher concentration of precursors of PA synthesis such as L-ornithine, agmatine, and S-adenosyl methionine, besides higher putrescine and cadaverine and lower spermidine during the 15 days of SMDS. AMF colonization raised the PA synthase (arginine decarboxylase, ornithine decarboxylase, spermidine synthase, and spermine synthase) activities and PA-degrading enzymes (copper-containing diamine oxidase and FAD-containing polyamine oxidase) in response to SMDS. However, mycorrhizal plants showed a relatively lower degree of membrane lipid peroxidation, superoxide anion free radical, and hydrogen peroxide than non-mycorrhizal plants, whereas the difference between them increased linearly up to 15 days of SMDS. Our study concluded that AMF regulated PA homeostasis in roots of Trifoliate Orange to tolerate SMDS.
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Effects of beneficial endophytic fungal inoculants on plant growth and nutrient absorption of Trifoliate Orange seedlings
Scientia Horticulturae, 2021Co-Authors: Liu Yang, Ying-ning Zou, Zhi-hong Tian, Kamil KucaAbstract:Abstract Citrus plants heavily depend on soil arbuscular mycorrhizal fungi (AMF), while the application of AMF in citrus is limited because of no propagation in vitro. The present work tried to apply a culturable (in vitro) endophytic fungus Piriformospora indica into Trifoliate Orange (Poncirus trifoliata) and to compare the role of P. indica in plant growth and nutrient acquisition especially phosphorus (P), relative to an arbuscular mycorrhizal fungus Funneliformis mosseae. After 20 weeks, roots of Trifoliate Orange could be colonized by P. indica, and both P. indica and F. mosseae existed in roots together. Root fungal colonization and soil easily extractable, difficultly extractable, and total glomalin-related protein concentrations were higher under single F. mosseae inoculation than under single P. indica and dual inoculation of F. mosseae and P. indica. Single or dual microbial inoculant collectively improved plant growth including plant height, leaf number, and leaf, stem, and root biomass, compared with non-inoculation. In addition, single fungal treatment raised the content of root N, P, and Mg, and dual inoculation increased only Mg and B contents. The improvement of P by single microbial inoculant was associated with the fungi-accelerated soil phosphatase activity and the fungi-induced root phosphate transporter genes (PT3, PT5, and PT6) expression levels. The positive effects on growth and nutrients were more significant under single F. mosseae than under single P. indica and dual inoculations. It concluded that P. indica has the stimulated roles in plant growth and P acquisition of Trifoliate Orange, and thus has more applied potentiality in citriculture than AMF as a biofertilizer.
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arbuscular mycorrhizal fungi alleviate drought stress in Trifoliate Orange by regulating h atpase activity and gene expression
Frontiers in Plant Science, 2021Co-Authors: Huiqian Cheng, Ying-ning Zou, Kamil KucaAbstract:A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, while it is unclear whether host H+-ATPase activity and gene expression are involved in the physiological process. The present study aimed to investigate the effects of an arbuscular mycorrhizal fungus (AMF), Funneliformis mosseae, on H+-ATPase activity and gene expression of Trifoliate Orange (Poncirus trifoliata) seedlings subjected to well-watered and drought stress (DS), together with the changes in leaf gas exchange, root morphology, soil pH value, and ammonium content. Soil drought treatment dramatically increased H+-ATPase activity of leaf and root, and AMF inoculation further strengthened the increased effect. A plasma membrane H+-ATPase gene of Trifoliate Orange, PtAHA2 (MW239123), was cloned. The PtAHA2 expression was induced by mycorrhization in leaves and roots and also up-regulated by drought treatment in leaves of AMF-inoculated seedlings and in roots of AMF- and non-AMF-inoculated seedlings. And, the induced expression of PtAHA2 under mycorrhization was more prominent under DS than under well-watered. Mycorrhizal plants also showed greater photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate and better root volume and diameter than non-mycorrhizal plants under DS. AMF inoculation significantly increased leaf and root ammonium content, especially under DS, whereas dramatically reduced soil pH value. In addition, H+-ATPase activity was significantly positively correlated with ammonium contents in leaves and roots, and root H+-ATPase activity was significantly negatively correlated with soil pH value. Our results concluded that AMF stimulated H+-ATPase activity and PtAHA2 gene expression in response to DS, which resulted in great nutrient (e.g. ammonium) uptake and root growth, as well as low soil pH microenvironment.
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Transcriptome analysis reveals improved root hair growth in Trifoliate Orange seedlings by arbuscular mycorrhizal fungi
Plant Growth Regulation, 2020Co-Authors: Chun-yan Liu, Dejian Zhang, Ying-ning Zou, Fei Zhang, Bo ShuAbstract:Arbuscular mycorrhizal fungi (AMF) regulate root hair formation in host plants, whereas the underlying mechanisms are unknown at the molecular level. The present study aimed to analyze the molecular change in Trifoliate Orange (Poncirus trifoliata) seedlings after inoculated with Clariodeoglomus etunicatum (C_e) and Funneliformis mosseae (F_m) for 3 months. We analyzed RNA sequences in lateral root tips from non-mycorrhizal and mycorrhizal plants, then randomly screened and verified 10 differentially expressed genes (DEGs) using quantitative real-time PCR (qRT-PCR). The results showed that both fungal species increased root hair density and length in all orders of root classes (except 2nd lateral roots in C_e treatment), as well as root biomass and phosphorus (P) content in leaves and roots. We identified 1300 and 1810 DEGs in plants inoculated with C_e and F_m and validated transcriptome data using qRT-PCR, where 21, 19, and 10 DEGs were associated with P, auxins, and expansins, respectively. The most downregulated and upregulated DEGs associated with P encoded acid phosphatase and phosphate transporter, respectively. The ABC transporter was the most upregulated DEG associated with auxin metabolism. Among the DEGs associated with expansins, 50% each were upregulated and downregulated. These findings revealed that mycorrhiza colonization elicited molecular changes in improved root hairs of Trifoliate Orange in association with phosphorus, auxin, and expansins.
Mohammed Mahabubur Rahman - One of the best experts on this subject based on the ideXlab platform.
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Effects of Common Mycorrhizal Network on Plant Carbohydrates and Soil Properties in Trifoliate Orange-White Clover Association.
PloS one, 2015Co-Authors: Zhang Zezhi, You-gen Lou, Dao-juan Deng, Mohammed Mahabubur RahmanAbstract:Common mycorrhizal network (CMN) allows nutrients and signals to pass between two or more plants. In this study, Trifoliate Orange (Poncirus trifoliata) and white clover (Trifolium repens) were planted in a two-compartmented rootbox, separated by a 37–μm nylon mesh and then inoculated with an arbuscular mycorrhizal fungus (AMF), Diversispora spurca. Inoculation with D. spurca resulted in formation of a CMN between Trifoliate Orange and white clover, whilst the best AM colonization occurred in the donor Trifoliate Orange–receptor white clover association. In the Trifoliate Orange–white clover association, the mycorrhizal colonization of receptor plant by extraradical hyphae originated from the donor plant significantly increased shoot and root fresh weight and chlorophyll concentration of the receptor plant. Enzymatic activity of soil β-glucoside hydrolase, protease, acid and neutral phosphatase, water-stable aggregate percentage at 2–4 and 0.5–1 mm size, and mean weight diameter in the rhizosphere of the receptor plant also increased. The hyphae of CMN released more easily-extractable glomalin-related soil protein and total glomalin-related soil protein into the receptor rhizosphere, which represented a significantly positive correlation with aggregate stability. AMF inoculation exhibited diverse changes in leaf and root sucrose concentration in the donor plant, and AM colonization by CMN conferred a significant increase of root glucose in the receptor plant. These results suggested that CMN formed in the Trifoliate Orange–white clover association, and root AM colonization by CMN promoted plant growth, root glucose accumulation, and rhizospheric soil properties in the receptor plant.
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Water-stable aggregate distribution in rhizosphere of Trifoliate Orange (a) and white clover (b) plants inoculated with Diversispora spurca in a two-compartmented rootbox separated by 37 μm nylon-mesh.
2015Co-Authors: Ze-zhi Zhang, You-gen Lou, Dao-juan Deng, Mohammed Mahabubur RahmanAbstract:Data (means ± SD, n = 4) followed by different letters indicate significant differences (P < 0.05) between treatments. Abbreviation: TO+/WC+, both Trifoliate Orange and white clover plants were inoculated with D. spurca; TO+/WC–, the donor plant Trifoliate Orange was inoculated with D. spurca, but the receptor plant white clover was not inoculated with D. spurca; TO–/WC+, the donor plant white clover was inoculated with D. spurca, but the receptor plant Trifoliate Orange was not inoculated with D. spurca; TO–/WC–, both Trifoliate Orange and white clover were not inoculated with D. spurca.
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Sucrose (a), glucose (b), and fructose (c) concentrations in leaf and root of Trifoliate Orange and white clover plants inoculated with Diversispora spurca in a two-compartmented rootbox separated by 37 μm nylon-mesh.
2015Co-Authors: Ze-zhi Zhang, You-gen Lou, Dao-juan Deng, Mohammed Mahabubur RahmanAbstract:Data (means ± SD, n = 4) followed by different letters indicate significant differences (P < 0.05) between treatments. Abbreviation: TO+/WC+, both Trifoliate Orange and white clover plants were inoculated with D. spurca; TO+/WC–, the donor plant Trifoliate Orange was inoculated with D. spurca, but the receptor plant white clover was not inoculated with D. spurca; TO–/WC+, the donor plant white clover was inoculated with D. spurca, but the receptor plant Trifoliate Orange was not inoculated with D. spurca; TO–/WC–, both Trifoliate Orange and white clover were not inoculated with D. spurca.
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Mean weight diameter (MWD) of water-stable aggregates in 0.25–4 mm size in rhizosphere of Trifoliate Orange and white clover plants inoculated with Diversispora spurca in a two-compartmented rootbox separated by 37 μm nylon-mesh.
2015Co-Authors: Ze-zhi Zhang, You-gen Lou, Dao-juan Deng, Mohammed Mahabubur RahmanAbstract:Data (means ± SD, n = 4) followed by different letters indicate significant differences (P < 0.05) between treatments. Abbreviation: TO+/WC+, both Trifoliate Orange and white clover plants were inoculated with D. spurca; TO+/WC–, the donor plant Trifoliate Orange was inoculated with D. spurca, but the receptor plant white clover was not inoculated with D. spurca; TO–/WC+, the donor plant white clover was inoculated with D. spurca, but the receptor plant Trifoliate Orange was not inoculated with D. spurca; TO–/WC–, both Trifoliate Orange and white clover were not inoculated with D. spurca.
Kamil Kuca - One of the best experts on this subject based on the ideXlab platform.
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mycorrhizal response strategies of Trifoliate Orange under well watered salt stress and waterlogging stress by regulating leaf aquaporin expression
Plant Physiology and Biochemistry, 2021Co-Authors: Xiaofen Cheng, Ying-ning Zou, Kamil KucaAbstract:Aquaporins (AQPs) involved in water and small molecule transport respond to environmental stress, while it is not clear how arbuscular mycorrhizal fungi (AMF) regulate AQP expression. Here, we investigated the change in leaf water potential and expression level of four tonoplast intrinsic proteins (TIPs), six plasma membrane intrinsic proteins (PIPs), and four nodin-26 like intrinsic proteins (NIPs) genes in Trifoliate Orange (Poncirus trifoliata) inoculated with Funneliformis mosseae under well-watered (WW), salt stress (SS), and waterlogging stress (WS). Root AMF colonization and soil hyphal length collectively were reduced by SS and WS. Under WW, inoculation with AMF gave diverse responses of AQPs: six AQPs up-regulated, three AQPs down-regulated, and five AQPs did not change. Such up-regulation of more AQPs under mycorrhization and WW partly accelerated water absorption, thereby, maintaining higher leaf water potential. However, under SS, all the fourteen AQPs were dramatically induced by AMF inoculation, which improved water permeability of membranes and stimulated water transport of the host. Under WS, AMF colonization almost did not induce or even down-regulated these AQPs expressions with three exceptions (PtTIP2;2, PtPIP1;1, and PtNIP1;2), thus, no change in leaf water potential. As a result, mycorrhizal plants under flooding may have an escape mechanism to reduce water absorption. It is concluded that AMF had different strategies in response to environmental stresses (e.g. SS and WS) by regulating leaf AQP expression in the host (e.g. Trifoliate Orange).
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Arbuscular Mycorrhizal Fungi Regulate Polyamine Homeostasis in Roots of Trifoliate Orange for Improved Adaptation to Soil Moisture Deficit Stress.
Frontiers in plant science, 2021Co-Authors: Ying-ning Zou, Fei Zhang, A. K. Srivastava, Kamil KucaAbstract:Soil arbuscular mycorrhizal fungi (AMF) enhance the tolerance of plants against soil moisture deficit stress (SMDS), but the underlying mechanisms are still not fully understood. Polyamines (PAs) as low-molecular-weight, aliphatic polycations have strong roles in abiotic stress tolerance of plants. We aimed to investigate the effect of AMF (Funneliformis mosseae) inoculation on PAs, PA precursors, activities of PA synthases and degrading enzymes, and concentration of reactive oxygen species in the roots of Trifoliate Orange (Poncirus trifoliata) subjected to 15 days of SMDS. Leaf water potential and total chlorophyll levels were comparatively higher in AMF-inoculated than in non-AMF-treated plants exposed to SMDS. Mycorrhizal plants recorded a significantly higher concentration of precursors of PA synthesis such as L-ornithine, agmatine, and S-adenosyl methionine, besides higher putrescine and cadaverine and lower spermidine during the 15 days of SMDS. AMF colonization raised the PA synthase (arginine decarboxylase, ornithine decarboxylase, spermidine synthase, and spermine synthase) activities and PA-degrading enzymes (copper-containing diamine oxidase and FAD-containing polyamine oxidase) in response to SMDS. However, mycorrhizal plants showed a relatively lower degree of membrane lipid peroxidation, superoxide anion free radical, and hydrogen peroxide than non-mycorrhizal plants, whereas the difference between them increased linearly up to 15 days of SMDS. Our study concluded that AMF regulated PA homeostasis in roots of Trifoliate Orange to tolerate SMDS.
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Effects of beneficial endophytic fungal inoculants on plant growth and nutrient absorption of Trifoliate Orange seedlings
Scientia Horticulturae, 2021Co-Authors: Liu Yang, Ying-ning Zou, Zhi-hong Tian, Kamil KucaAbstract:Abstract Citrus plants heavily depend on soil arbuscular mycorrhizal fungi (AMF), while the application of AMF in citrus is limited because of no propagation in vitro. The present work tried to apply a culturable (in vitro) endophytic fungus Piriformospora indica into Trifoliate Orange (Poncirus trifoliata) and to compare the role of P. indica in plant growth and nutrient acquisition especially phosphorus (P), relative to an arbuscular mycorrhizal fungus Funneliformis mosseae. After 20 weeks, roots of Trifoliate Orange could be colonized by P. indica, and both P. indica and F. mosseae existed in roots together. Root fungal colonization and soil easily extractable, difficultly extractable, and total glomalin-related protein concentrations were higher under single F. mosseae inoculation than under single P. indica and dual inoculation of F. mosseae and P. indica. Single or dual microbial inoculant collectively improved plant growth including plant height, leaf number, and leaf, stem, and root biomass, compared with non-inoculation. In addition, single fungal treatment raised the content of root N, P, and Mg, and dual inoculation increased only Mg and B contents. The improvement of P by single microbial inoculant was associated with the fungi-accelerated soil phosphatase activity and the fungi-induced root phosphate transporter genes (PT3, PT5, and PT6) expression levels. The positive effects on growth and nutrients were more significant under single F. mosseae than under single P. indica and dual inoculations. It concluded that P. indica has the stimulated roles in plant growth and P acquisition of Trifoliate Orange, and thus has more applied potentiality in citriculture than AMF as a biofertilizer.
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arbuscular mycorrhizal fungi alleviate drought stress in Trifoliate Orange by regulating h atpase activity and gene expression
Frontiers in Plant Science, 2021Co-Authors: Huiqian Cheng, Ying-ning Zou, Kamil KucaAbstract:A feature of arbuscular mycorrhiza is enhanced drought tolerance of host plants, while it is unclear whether host H+-ATPase activity and gene expression are involved in the physiological process. The present study aimed to investigate the effects of an arbuscular mycorrhizal fungus (AMF), Funneliformis mosseae, on H+-ATPase activity and gene expression of Trifoliate Orange (Poncirus trifoliata) seedlings subjected to well-watered and drought stress (DS), together with the changes in leaf gas exchange, root morphology, soil pH value, and ammonium content. Soil drought treatment dramatically increased H+-ATPase activity of leaf and root, and AMF inoculation further strengthened the increased effect. A plasma membrane H+-ATPase gene of Trifoliate Orange, PtAHA2 (MW239123), was cloned. The PtAHA2 expression was induced by mycorrhization in leaves and roots and also up-regulated by drought treatment in leaves of AMF-inoculated seedlings and in roots of AMF- and non-AMF-inoculated seedlings. And, the induced expression of PtAHA2 under mycorrhization was more prominent under DS than under well-watered. Mycorrhizal plants also showed greater photosynthetic rate, stomatal conductance, intercellular CO2 concentration, and transpiration rate and better root volume and diameter than non-mycorrhizal plants under DS. AMF inoculation significantly increased leaf and root ammonium content, especially under DS, whereas dramatically reduced soil pH value. In addition, H+-ATPase activity was significantly positively correlated with ammonium contents in leaves and roots, and root H+-ATPase activity was significantly negatively correlated with soil pH value. Our results concluded that AMF stimulated H+-ATPase activity and PtAHA2 gene expression in response to DS, which resulted in great nutrient (e.g. ammonium) uptake and root growth, as well as low soil pH microenvironment.
