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Gianfranco Mamone - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp. Seed Germ flour
Food research international (Ottawa Ont.), 2019Co-Authors: Gianfranco Mamone, Francesco Siano, Leonardo Pablo Sciammaro, Gianluca Picariello, Salvatore De Caro, Luigia Di Stasio, Maria Cecilia PuppoAbstract:By virtue of exclusive nutrient composition and nutritional properties, Seed Germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations. In order to define their compositional and functional properties, we investigated the Germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods. The mono- and two-dimensional electrophoretic profiles of Prosopis spp. were very similar among one another, while C. siliqua exhibited significant differences. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob Germ flour might more suited than Prosopis Germ flour for baking and food technological applications. In contrast, Prosopis spp. contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement. Both carob and algarrobo Germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
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integrated analytical methods to characterize lipids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Analytical Methods, 2018Co-Authors: Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Gianfranco Mamone, Maria Cecilia Puppo, Gianluca PicarielloAbstract:Flour from Seed Germ of European carob (Ceratonia siliqua) and South American algarrobo (Prosopis spp.) is a potential ingredient for health-promoting baked products. Herein, lipids from Germ of three Argentinean Prosopis (P. alba, P. nigra, and P. ruscifolia) and one European carob species were characterized in detail, exploiting an array of up-to-date analytical techniques. Total lipids ranged from 7.1 to 8.1% (w/w). Linoleic acid (C18:2, ω-6) predominated the GC flame ionization detector profiles of fatty acid in all samples (43.3–50.6%). Prosopis spp. contained 6–7% of C20–C24 fatty acids and, consistently, C56–C60 triacylglycerols, as detected by MALDI-TOF mass spectrometry (MS), which were practically missing in C. siliqua Germ. Phospholipids were isolated by hydroxyapatite chromatography, characterized by MALDI-TOF MS and grossly quantified by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Because of a relatively high content (9.5–11.8%, v/v), phospholipids might increase the antioxidant potential and improve the baking performances of flour fortified with carob and algarrobo Seed Germ.
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comparative analysis of c glycosidic flavonoids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Research International, 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
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Comparative analysis of C-glycosidic flavonoids from Prosopis spp. and Ceratonia siliqua Seed Germ flour.
Food research international (Ottawa Ont.), 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
Gianluca Picariello - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp. Seed Germ flour
Food research international (Ottawa Ont.), 2019Co-Authors: Gianfranco Mamone, Francesco Siano, Leonardo Pablo Sciammaro, Gianluca Picariello, Salvatore De Caro, Luigia Di Stasio, Maria Cecilia PuppoAbstract:By virtue of exclusive nutrient composition and nutritional properties, Seed Germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations. In order to define their compositional and functional properties, we investigated the Germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods. The mono- and two-dimensional electrophoretic profiles of Prosopis spp. were very similar among one another, while C. siliqua exhibited significant differences. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob Germ flour might more suited than Prosopis Germ flour for baking and food technological applications. In contrast, Prosopis spp. contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement. Both carob and algarrobo Germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
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integrated analytical methods to characterize lipids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Analytical Methods, 2018Co-Authors: Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Gianfranco Mamone, Maria Cecilia Puppo, Gianluca PicarielloAbstract:Flour from Seed Germ of European carob (Ceratonia siliqua) and South American algarrobo (Prosopis spp.) is a potential ingredient for health-promoting baked products. Herein, lipids from Germ of three Argentinean Prosopis (P. alba, P. nigra, and P. ruscifolia) and one European carob species were characterized in detail, exploiting an array of up-to-date analytical techniques. Total lipids ranged from 7.1 to 8.1% (w/w). Linoleic acid (C18:2, ω-6) predominated the GC flame ionization detector profiles of fatty acid in all samples (43.3–50.6%). Prosopis spp. contained 6–7% of C20–C24 fatty acids and, consistently, C56–C60 triacylglycerols, as detected by MALDI-TOF mass spectrometry (MS), which were practically missing in C. siliqua Germ. Phospholipids were isolated by hydroxyapatite chromatography, characterized by MALDI-TOF MS and grossly quantified by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Because of a relatively high content (9.5–11.8%, v/v), phospholipids might increase the antioxidant potential and improve the baking performances of flour fortified with carob and algarrobo Seed Germ.
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comparative analysis of c glycosidic flavonoids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Research International, 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
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Comparative analysis of C-glycosidic flavonoids from Prosopis spp. and Ceratonia siliqua Seed Germ flour.
Food research international (Ottawa Ont.), 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
Maria Cecilia Puppo - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp. Seed Germ flour
Food research international (Ottawa Ont.), 2019Co-Authors: Gianfranco Mamone, Francesco Siano, Leonardo Pablo Sciammaro, Gianluca Picariello, Salvatore De Caro, Luigia Di Stasio, Maria Cecilia PuppoAbstract:By virtue of exclusive nutrient composition and nutritional properties, Seed Germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations. In order to define their compositional and functional properties, we investigated the Germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods. The mono- and two-dimensional electrophoretic profiles of Prosopis spp. were very similar among one another, while C. siliqua exhibited significant differences. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob Germ flour might more suited than Prosopis Germ flour for baking and food technological applications. In contrast, Prosopis spp. contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement. Both carob and algarrobo Germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
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integrated analytical methods to characterize lipids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Analytical Methods, 2018Co-Authors: Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Gianfranco Mamone, Maria Cecilia Puppo, Gianluca PicarielloAbstract:Flour from Seed Germ of European carob (Ceratonia siliqua) and South American algarrobo (Prosopis spp.) is a potential ingredient for health-promoting baked products. Herein, lipids from Germ of three Argentinean Prosopis (P. alba, P. nigra, and P. ruscifolia) and one European carob species were characterized in detail, exploiting an array of up-to-date analytical techniques. Total lipids ranged from 7.1 to 8.1% (w/w). Linoleic acid (C18:2, ω-6) predominated the GC flame ionization detector profiles of fatty acid in all samples (43.3–50.6%). Prosopis spp. contained 6–7% of C20–C24 fatty acids and, consistently, C56–C60 triacylglycerols, as detected by MALDI-TOF mass spectrometry (MS), which were practically missing in C. siliqua Germ. Phospholipids were isolated by hydroxyapatite chromatography, characterized by MALDI-TOF MS and grossly quantified by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Because of a relatively high content (9.5–11.8%, v/v), phospholipids might increase the antioxidant potential and improve the baking performances of flour fortified with carob and algarrobo Seed Germ.
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comparative analysis of c glycosidic flavonoids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Research International, 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
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Comparative analysis of C-glycosidic flavonoids from Prosopis spp. and Ceratonia siliqua Seed Germ flour.
Food research international (Ottawa Ont.), 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
Francesco Siano - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp. Seed Germ flour
Food research international (Ottawa Ont.), 2019Co-Authors: Gianfranco Mamone, Francesco Siano, Leonardo Pablo Sciammaro, Gianluca Picariello, Salvatore De Caro, Luigia Di Stasio, Maria Cecilia PuppoAbstract:By virtue of exclusive nutrient composition and nutritional properties, Seed Germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations. In order to define their compositional and functional properties, we investigated the Germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods. The mono- and two-dimensional electrophoretic profiles of Prosopis spp. were very similar among one another, while C. siliqua exhibited significant differences. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob Germ flour might more suited than Prosopis Germ flour for baking and food technological applications. In contrast, Prosopis spp. contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement. Both carob and algarrobo Germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
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integrated analytical methods to characterize lipids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Analytical Methods, 2018Co-Authors: Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Gianfranco Mamone, Maria Cecilia Puppo, Gianluca PicarielloAbstract:Flour from Seed Germ of European carob (Ceratonia siliqua) and South American algarrobo (Prosopis spp.) is a potential ingredient for health-promoting baked products. Herein, lipids from Germ of three Argentinean Prosopis (P. alba, P. nigra, and P. ruscifolia) and one European carob species were characterized in detail, exploiting an array of up-to-date analytical techniques. Total lipids ranged from 7.1 to 8.1% (w/w). Linoleic acid (C18:2, ω-6) predominated the GC flame ionization detector profiles of fatty acid in all samples (43.3–50.6%). Prosopis spp. contained 6–7% of C20–C24 fatty acids and, consistently, C56–C60 triacylglycerols, as detected by MALDI-TOF mass spectrometry (MS), which were practically missing in C. siliqua Germ. Phospholipids were isolated by hydroxyapatite chromatography, characterized by MALDI-TOF MS and grossly quantified by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Because of a relatively high content (9.5–11.8%, v/v), phospholipids might increase the antioxidant potential and improve the baking performances of flour fortified with carob and algarrobo Seed Germ.
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comparative analysis of c glycosidic flavonoids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Research International, 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
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Comparative analysis of C-glycosidic flavonoids from Prosopis spp. and Ceratonia siliqua Seed Germ flour.
Food research international (Ottawa Ont.), 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
Leonardo Pablo Sciammaro - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp. Seed Germ flour
Food research international (Ottawa Ont.), 2019Co-Authors: Gianfranco Mamone, Francesco Siano, Leonardo Pablo Sciammaro, Gianluca Picariello, Salvatore De Caro, Luigia Di Stasio, Maria Cecilia PuppoAbstract:By virtue of exclusive nutrient composition and nutritional properties, Seed Germ flours from both European carob (Ceratonia siliqua L.) and South American algarrobo (Prosopis alba and Prosopis nigra) or vinal (Prosopis ruscifolia) have potential as a high nutritional value and health-promoting ingredient for food formulations. In order to define their compositional and functional properties, we investigated the Germ protein content of carob compared to the P. alba, P. nigra and P. ruscifolia counterparts, applying proteomics and complementary methods. The mono- and two-dimensional electrophoretic profiles of Prosopis spp. were very similar among one another, while C. siliqua exhibited significant differences. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy highlighted a dominant β-sheet structural conformation for C. siliqua, suggesting that carob Germ flour might more suited than Prosopis Germ flour for baking and food technological applications. In contrast, Prosopis spp. contained a more adequate nutritional value than C. siliqua, in terms of essential amino acid complement. Both carob and algarrobo Germ flour samples were highly digestible, as demonstrated by in vitro simulated gastrointestinal digestion, releasing high amounts of free amino acids and only minor proportions of low molecular weight peptides.
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integrated analytical methods to characterize lipids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Analytical Methods, 2018Co-Authors: Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Gianfranco Mamone, Maria Cecilia Puppo, Gianluca PicarielloAbstract:Flour from Seed Germ of European carob (Ceratonia siliqua) and South American algarrobo (Prosopis spp.) is a potential ingredient for health-promoting baked products. Herein, lipids from Germ of three Argentinean Prosopis (P. alba, P. nigra, and P. ruscifolia) and one European carob species were characterized in detail, exploiting an array of up-to-date analytical techniques. Total lipids ranged from 7.1 to 8.1% (w/w). Linoleic acid (C18:2, ω-6) predominated the GC flame ionization detector profiles of fatty acid in all samples (43.3–50.6%). Prosopis spp. contained 6–7% of C20–C24 fatty acids and, consistently, C56–C60 triacylglycerols, as detected by MALDI-TOF mass spectrometry (MS), which were practically missing in C. siliqua Germ. Phospholipids were isolated by hydroxyapatite chromatography, characterized by MALDI-TOF MS and grossly quantified by attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy. Because of a relatively high content (9.5–11.8%, v/v), phospholipids might increase the antioxidant potential and improve the baking performances of flour fortified with carob and algarrobo Seed Germ.
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comparative analysis of c glycosidic flavonoids from prosopis spp and ceratonia siliqua Seed Germ flour
Food Research International, 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).
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Comparative analysis of C-glycosidic flavonoids from Prosopis spp. and Ceratonia siliqua Seed Germ flour.
Food research international (Ottawa Ont.), 2017Co-Authors: Gianluca Picariello, Francesco Siano, Leonardo Pablo Sciammaro, Maria Grazia Volpe, Maria Cecilia Puppo, Gianfranco MamoneAbstract:Abstract Seed Germ of South American algarrobo ( Prosopis species) and European carob ( Ceratonia siliqua ) contains nutritionally interesting proteins, lipids and phenolics. Using reversed phase-HPLC-diode array detector and nanoflow-HPLC coupled to tandem mass spectrometry (MS/MS), we comparatively characterized and semi-quantified flavonoids from Germ of three Argentinean algarrobo ( Prosopis alba , Prosopis nigra and Prosopis ruscifolia ) and one European carob species. The patterns of glycosylated flavonoids were very similar each other, confirming the taxonomic parentage of the species and supporting their functional similarity on a molecular basis, in view of the use of Seed Germ flour (SGF) for food applications. The predominant phenolic compounds were apigenin 6,8- C -di-glycoside isomers, namely isoschaftoside and schaftoside, accounting for 3.22–5.18 and 0.41–0.72 mg/g SGF, respectively. C. siliqua Germ contained relatively high amounts of further glycosilated derivatives of (iso)schaftoside, which occurred at a lower abundance in Prosopis . Apigenin 6,8- C -di-glycosides have been described as potent α-glucosidase inhibitors, suggesting that food preparations obtained with Prosopis spp. and C. siliqua SGF might contribute to modulate the digestion of carbohydrates in humans. Chemical compounds Isoschaftoside (PubChem CID: 13644661); Schaftoside (PubChem CID: 442658); Vicenin-2 (PubChem CID: 442664); Isovitexin (PubChem CID: 162350).