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Barry J Jacobsen - One of the best experts on this subject based on the ideXlab platform.
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:ABSTRACT A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling diseas...
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling Diseases of sugar beet (Beta vulgaris) caused by Pythium ultimum, Rhizoctonia solani AG 2-2, and Aphanomyces cochlioides. The biorational mixture provided better control than the live M. albus formulation for control of root-knot nematode, Meloidogyne incognita, on tomato (Lycopersicon esculentum). The biorational mixture provided control of damping-off equal to a starch-based formulation of the live fungus for all three sugar beet pathogens, and significantly reduced the number of root-knot galls on tomato roots compared with a barley-based formulation. Rate studies with the biorational mixture showed that 2 and 0.75 µl/cm3 of soil were required to provide optimal control of Rhizoctonia and Pythium damping-off of sugar beet, respectively. Five microliters of biorational mixture per milliliter of water was required for 100% mortality in 24 h for Meloidogyne incognita in in vitro studies. In in vivo studies, 1.67 µl of the biorational mixture/cm3 of sand resulted in fewer root-knot galls than a Muscodor albus infested ground barley formulation applied at 5 g/liter of sand.
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mycofumigation with muscodor albus and muscodor roseus for control of Seedling Diseases of sugar beet and verticillium wilt of eggplant
Plant Disease, 2003Co-Authors: A M Stinson, Nina K Zidack, G A Strobel, Barry J JacobsenAbstract:ABSTRACT Mycofumigation is the use of antimicrobial volatiles produced by fungi such as Muscodor albusitalic and M. roseus for the control of other organisms. Sugar beet (Beta vulgaris L.) stand establishment was increased and disease severity decreased by mycofumigation with M. roseus and M. albus in autoclaved soil infested with Rhizoctonia solani, Pythium ultimum, or Aphanomyces cochlioides. Eggplant Seedlings (Solanum melongena L.) transplanted into autoclaved soil infested with Verticillium dahliae and mycofumigated with M. albus and M. roseus had significantly less disease (P < 0.05) after 4 and 5 weeks compared with nonmycofumigated Verticillium-infested soil. The effect of formulation on efficacy of mycofumigation with M. roseus was tested using potato dextrose agar strips, alginate capsules, ground barley, pesta granules, and stabileze granules. The stabileze and ground barley formulations of M. roseus resulted in the best control of P. ultimum damping-off. The best control of A. cochlioides damp...
E Grimme - One of the best experts on this subject based on the ideXlab platform.
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:ABSTRACT A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling diseas...
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling Diseases of sugar beet (Beta vulgaris) caused by Pythium ultimum, Rhizoctonia solani AG 2-2, and Aphanomyces cochlioides. The biorational mixture provided better control than the live M. albus formulation for control of root-knot nematode, Meloidogyne incognita, on tomato (Lycopersicon esculentum). The biorational mixture provided control of damping-off equal to a starch-based formulation of the live fungus for all three sugar beet pathogens, and significantly reduced the number of root-knot galls on tomato roots compared with a barley-based formulation. Rate studies with the biorational mixture showed that 2 and 0.75 µl/cm3 of soil were required to provide optimal control of Rhizoctonia and Pythium damping-off of sugar beet, respectively. Five microliters of biorational mixture per milliliter of water was required for 100% mortality in 24 h for Meloidogyne incognita in in vitro studies. In in vivo studies, 1.67 µl of the biorational mixture/cm3 of sand resulted in fewer root-knot galls than a Muscodor albus infested ground barley formulation applied at 5 g/liter of sand.
G A Strobel - One of the best experts on this subject based on the ideXlab platform.
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:ABSTRACT A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling diseas...
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling Diseases of sugar beet (Beta vulgaris) caused by Pythium ultimum, Rhizoctonia solani AG 2-2, and Aphanomyces cochlioides. The biorational mixture provided better control than the live M. albus formulation for control of root-knot nematode, Meloidogyne incognita, on tomato (Lycopersicon esculentum). The biorational mixture provided control of damping-off equal to a starch-based formulation of the live fungus for all three sugar beet pathogens, and significantly reduced the number of root-knot galls on tomato roots compared with a barley-based formulation. Rate studies with the biorational mixture showed that 2 and 0.75 µl/cm3 of soil were required to provide optimal control of Rhizoctonia and Pythium damping-off of sugar beet, respectively. Five microliters of biorational mixture per milliliter of water was required for 100% mortality in 24 h for Meloidogyne incognita in in vitro studies. In in vivo studies, 1.67 µl of the biorational mixture/cm3 of sand resulted in fewer root-knot galls than a Muscodor albus infested ground barley formulation applied at 5 g/liter of sand.
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mycofumigation with muscodor albus and muscodor roseus for control of Seedling Diseases of sugar beet and verticillium wilt of eggplant
Plant Disease, 2003Co-Authors: A M Stinson, Nina K Zidack, G A Strobel, Barry J JacobsenAbstract:ABSTRACT Mycofumigation is the use of antimicrobial volatiles produced by fungi such as Muscodor albusitalic and M. roseus for the control of other organisms. Sugar beet (Beta vulgaris L.) stand establishment was increased and disease severity decreased by mycofumigation with M. roseus and M. albus in autoclaved soil infested with Rhizoctonia solani, Pythium ultimum, or Aphanomyces cochlioides. Eggplant Seedlings (Solanum melongena L.) transplanted into autoclaved soil infested with Verticillium dahliae and mycofumigated with M. albus and M. roseus had significantly less disease (P < 0.05) after 4 and 5 weeks compared with nonmycofumigated Verticillium-infested soil. The effect of formulation on efficacy of mycofumigation with M. roseus was tested using potato dextrose agar strips, alginate capsules, ground barley, pesta granules, and stabileze granules. The stabileze and ground barley formulations of M. roseus resulted in the best control of P. ultimum damping-off. The best control of A. cochlioides damp...
Nina K Zidack - One of the best experts on this subject based on the ideXlab platform.
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:ABSTRACT A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling diseas...
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comparison of muscodor albus volatiles with a biorational mixture for control of Seedling Diseases of sugar beet and root knot nematode on tomato
Plant Disease, 2007Co-Authors: E Grimme, Nina K Zidack, R A Sikora, G A Strobel, Barry J JacobsenAbstract:A biorational synthetic mixture of organic components mimicking key antimicrobial gases produced by Muscodor albus was equivalent to the use of live M. albus for control of Seedling Diseases of sugar beet (Beta vulgaris) caused by Pythium ultimum, Rhizoctonia solani AG 2-2, and Aphanomyces cochlioides. The biorational mixture provided better control than the live M. albus formulation for control of root-knot nematode, Meloidogyne incognita, on tomato (Lycopersicon esculentum). The biorational mixture provided control of damping-off equal to a starch-based formulation of the live fungus for all three sugar beet pathogens, and significantly reduced the number of root-knot galls on tomato roots compared with a barley-based formulation. Rate studies with the biorational mixture showed that 2 and 0.75 µl/cm3 of soil were required to provide optimal control of Rhizoctonia and Pythium damping-off of sugar beet, respectively. Five microliters of biorational mixture per milliliter of water was required for 100% mortality in 24 h for Meloidogyne incognita in in vitro studies. In in vivo studies, 1.67 µl of the biorational mixture/cm3 of sand resulted in fewer root-knot galls than a Muscodor albus infested ground barley formulation applied at 5 g/liter of sand.
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mycofumigation with muscodor albus and muscodor roseus for control of Seedling Diseases of sugar beet and verticillium wilt of eggplant
Plant Disease, 2003Co-Authors: A M Stinson, Nina K Zidack, G A Strobel, Barry J JacobsenAbstract:ABSTRACT Mycofumigation is the use of antimicrobial volatiles produced by fungi such as Muscodor albusitalic and M. roseus for the control of other organisms. Sugar beet (Beta vulgaris L.) stand establishment was increased and disease severity decreased by mycofumigation with M. roseus and M. albus in autoclaved soil infested with Rhizoctonia solani, Pythium ultimum, or Aphanomyces cochlioides. Eggplant Seedlings (Solanum melongena L.) transplanted into autoclaved soil infested with Verticillium dahliae and mycofumigated with M. albus and M. roseus had significantly less disease (P < 0.05) after 4 and 5 weeks compared with nonmycofumigated Verticillium-infested soil. The effect of formulation on efficacy of mycofumigation with M. roseus was tested using potato dextrose agar strips, alginate capsules, ground barley, pesta granules, and stabileze granules. The stabileze and ground barley formulations of M. roseus resulted in the best control of P. ultimum damping-off. The best control of A. cochlioides damp...
A Elhaitoum - One of the best experts on this subject based on the ideXlab platform.
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first report of fusarium chlamydosporum causing damping off disease on aleppo pine in algeria
Plant Disease, 2013Co-Authors: F Lazreg, Lakhdar Belabid, J Sanchez, Eduardo Gallego, Jose Antonio Garridocardenas, A ElhaitoumAbstract:The Aleppo pine (Pinus halepensis Mill.) is a conifer native to the Mediterranean region. In 2008 and 2009, a survey of Aleppo pine Seedling Diseases was performed in three forest nurseries from the Relizane, Sidi Bel Abbes, and Tlemcen departments in northwestern Algeria. One- to two-month-old Aleppo pine Seedlings showed symptoms of damping-off in pre- and post-emergence (typical Seedling collar rot). The problem was widespread with a disease incidence of 64 to 77% and an annual impact of US$50,000. Disinfested root and root collar segments (from four composite samples per location), approximately 5 mm in length, were cultured on PDA and incubated at 25°C and day/night light. Two (from 21) isolates were identified morphologically (2) as the anamorph Fusarium chlamydosporum Wollenw. & Reinking and isolated from collar rots of Relizane forest nursery Seedlings. Colony development on PDA media was fast; 32 mm diameter colonies developed after 3 days. Colonies were white. Mycelia were floccose, fairly dense, off-white, and turned a lilac color in older portions of the colony. Macroconidia were thick-walled and moderately curved with unequal dorsiventral curvature (the lower wall is almost straight), short, curved and pointed apical cell, usually notched, but occasionally foot shaped basal cell, 3- to 5-septate, and 2 × 8 to 21 μm. Microconidia were abundant, 0-septate, and 2 × 6 to 9 μm. Chlamydospores were abundant, formed rapidly in single chains or clusters, and 8 to 15 μm diameter. To confirm the identity of this fungus, the internal transcribed spacer of F12RR and F4SR isolates of F. chlamydosporum were amplified and sequenced using ITS1 and ITS4 primers (4). Sequences were deposited in GenBank under accessions JX114795 and JX114789, respectively. Those sequences bore 99% similarity with reference sequence AY213655 (2) and 100% with HQ671187, also found 99 to 100% similarity with F. equiseti (Corda) Sacc. but with different conidia. Pathogenicity tests were performed to fulfill Koch's postulates. Inoculum was produced by adding a 5 mm diam. plug from a 7-day-old CMA petri dish culture to a previously sterilized 500 ml flask (237.5 g sand, 12.5 g cornmeal, 80 ml SDW), shaken over 9 days, and mixed with sterile soil at 1:3 (v:v). Infested soil was then transferred to 500 ml pots, and 10 seeds were planted. A completely randomized design was used with three replicates per isolate and three control pots. After 1 month, two tested isolates caused typical damping-off symptoms on Seedlings. The percentage of the plants that became infected was 65 to 77%. To our knowledge (1,3), this is the first report of F. chlamydosporum on Aleppo pine in northwestern Algeria. It is also the first report of this fungal species affecting the Aleppo pine throughout the world, and on conifers in Africa and the Mediterranean region (1,3). References: (1) D. F. Farr and A. Y. Rossman. Fungal Databases, Syst. Mycol. Microbiol. Lab. ARS, USDA, Beltsville, MD. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ , February 20, 2013. (2) J. F. Leslie and B. A. Summerell. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA, 2006. (3) D. W. Minter. Cybertruffle's Robigalia, Observations of Fungi and their Associated Organisms. Retrieved from http://www.cybertruffle.org.uk/robigalia/eng/ , February 20, 2013. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
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first report of fusarium equiseti causing damping off disease on aleppo pine in algeria
Plant Disease, 2013Co-Authors: F Lazreg, Lakhdar Belabid, J Sanchez, Eduardo Gallego, Jose Antonio Garridocardenas, A ElhaitoumAbstract:The Aleppo pine (Pinus halepensis Mill.) is a conifer native to the Mediterranean region. In 2008 and 2009, a survey of Aleppo pine Seedling Diseases was performed in three forest nurseries from Relizane, Sidi Bel Abbes, and Tlemcen provinces in northwestern Algeria. Aleppo pine Seedlings showed symptoms of pre- and post-emergence damping-off disease, with an incidence of 64 to 77%. Four composite samples were taken from each location. Disinfested root and root collar segments, approximately 5 mm in length, were cultured on potato dextrose agar (PDA) and incubated at 25°C, and hyphal tips were transferred to PDA. Fusarium equiseti (Corda) Sacc. (teleomorph: Gibberella intricans Wollenw.) was identified from roots of two Seedlings from the Sidi Bel Abbes nursery. Morphological identification was done according to Fusarium keys (2). PDA colonies with abundant, loosely floccose, whitish aerial mycelium and beige pigmentation were observed. Macroconidia with usually 5 to 6 septa, 31 to 45 μm long. A pronounced dorsiventral curvature, tapered and elongated apical cell, and prominent foot shape were observed. Microconidia were absent. Chlamydospores were produced in hyphae, most often intercalary, solitary, in pairs, frequently forming chains or clusters, globose (7 to 13 μm). To confirm the identity of this fungus, the internal transcribed spacer of F3RS1 and F19RS1 isolates of F. equiseti were amplified and sequenced using ITS1 and ITS4 primers (4), GenBank accession nos. JX114784 and JX114791, respectively. Those sequences bore 100% (HQ671182) similarity with sequences of F. equiseti in GenBank. Pathogenicity tests were performed to fulfill Koch's postulates. Inoculum was produced by adding a 5-mm-diameter plug from a 7-day-old CMA petri dish culture to a previously sterilized 500 ml flask (237.5 g sand, 12.5 g cornmeal, 80 ml sterile distilled water), shaken over 9 days at 25°C, and mixed with sterile sandy clay soil at 1:3 (v:v). Infested soil was then transferred to 500 ml pots, and 10 Aleppo pine seeds were planted per pot. A completely randomized design was used with three replicates per isolate and three control pots with a similar non-infested soil. After 1 month at 25°C the two tested isolates caused typical damping-off symptoms (collar rot) on Seedlings and were re-isolated from recently infected tissues. The percentages of the inoculated plants that became infected were 59 to 65% among isolates (0% in control pots). To our knowledge, infection by F. equiseti is a first report on Aleppo pine in northwestern Algeria, Northern Africa, and globally, and on conifers in the Mediterranean region (1,3). In Algeria, F. equiseti is associated with black pepper (Piper nigrum L.) (3). These findings highlight the moderate impact of F. equiseti on the production of Aleppo Seedling stock for reforestation activities in northwestern Algeria. References: (1) D. F. Farr and A. Y. Rossman. Fungal Databases, Systematic Mycology and Microbiology Laboratory. ARS, USDA, Beltsville, MD. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ , February 20, 2013. (2) J. F. Leslie and B. A. Summerell. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA, 2006. (3) D. W. Minter. Cybertruffle's Robigalia, Observations of Fungi and their Associated Organisms. Retrieved from http://www.cybertruffle.org.uk/robigalia/eng/ , February 20, 2013. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.