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Osamu Iwata - One of the best experts on this subject based on the ideXlab platform.
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Osamu IwataAbstract:Efficient Selective Breeding of live oil-rich Euglena gracilis with fluorescence-activated cell sorting
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Tomoko Abe, Osamu IwataAbstract:Euglena gracilis, a microalgal species of unicellular flagellate protists, has attracted much attention in both the industrial and academic sectors due to recent advances in the mass cultivation of E. gracilis that have enabled the cost-effective production of nutritional food and cosmetic commodities. In addition, it is known to produce paramylon (β-1,3-glucan in a crystalline form) as reserve polysaccharide and convert it to wax ester in hypoxic and anaerobic conditions-a promising feedstock for biodiesel and aviation biofuel. However, there remain a number of technical challenges to be solved before it can be deployed in the competitive fuel market. Here we present a method for efficient Selective Breeding of live oil-rich E. gracilis with fluorescence-activated cell sorting (FACS). Specifically, the Selective Breeding method is a repetitive procedure for one-week heterotrophic cultivation, staining intracellular lipids with BODIPY(505/515), and FACS-based isolation of top 0.5% lipid-rich E. gracilis cells with high viability, after inducing mutation with Fe-ion irradiation to the wild type (WT). Consequently, we acquire a live, stable, lipid-rich E. gracilis mutant strain, named B1ZFeL, with 40% more lipid content on average than the WT. Our method paves the way for rapid, cost-effective, energy-efficient production of biofuel.
Keisuke Goda - One of the best experts on this subject based on the ideXlab platform.
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Osamu IwataAbstract:Efficient Selective Breeding of live oil-rich Euglena gracilis with fluorescence-activated cell sorting
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Tomoko Abe, Osamu IwataAbstract:Euglena gracilis, a microalgal species of unicellular flagellate protists, has attracted much attention in both the industrial and academic sectors due to recent advances in the mass cultivation of E. gracilis that have enabled the cost-effective production of nutritional food and cosmetic commodities. In addition, it is known to produce paramylon (β-1,3-glucan in a crystalline form) as reserve polysaccharide and convert it to wax ester in hypoxic and anaerobic conditions-a promising feedstock for biodiesel and aviation biofuel. However, there remain a number of technical challenges to be solved before it can be deployed in the competitive fuel market. Here we present a method for efficient Selective Breeding of live oil-rich E. gracilis with fluorescence-activated cell sorting (FACS). Specifically, the Selective Breeding method is a repetitive procedure for one-week heterotrophic cultivation, staining intracellular lipids with BODIPY(505/515), and FACS-based isolation of top 0.5% lipid-rich E. gracilis cells with high viability, after inducing mutation with Fe-ion irradiation to the wild type (WT). Consequently, we acquire a live, stable, lipid-rich E. gracilis mutant strain, named B1ZFeL, with 40% more lipid content on average than the WT. Our method paves the way for rapid, cost-effective, energy-efficient production of biofuel.
Koji Yamada - One of the best experts on this subject based on the ideXlab platform.
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Osamu IwataAbstract:Efficient Selective Breeding of live oil-rich Euglena gracilis with fluorescence-activated cell sorting
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Tomoko Abe, Osamu IwataAbstract:Euglena gracilis, a microalgal species of unicellular flagellate protists, has attracted much attention in both the industrial and academic sectors due to recent advances in the mass cultivation of E. gracilis that have enabled the cost-effective production of nutritional food and cosmetic commodities. In addition, it is known to produce paramylon (β-1,3-glucan in a crystalline form) as reserve polysaccharide and convert it to wax ester in hypoxic and anaerobic conditions-a promising feedstock for biodiesel and aviation biofuel. However, there remain a number of technical challenges to be solved before it can be deployed in the competitive fuel market. Here we present a method for efficient Selective Breeding of live oil-rich E. gracilis with fluorescence-activated cell sorting (FACS). Specifically, the Selective Breeding method is a repetitive procedure for one-week heterotrophic cultivation, staining intracellular lipids with BODIPY(505/515), and FACS-based isolation of top 0.5% lipid-rich E. gracilis cells with high viability, after inducing mutation with Fe-ion irradiation to the wild type (WT). Consequently, we acquire a live, stable, lipid-rich E. gracilis mutant strain, named B1ZFeL, with 40% more lipid content on average than the WT. Our method paves the way for rapid, cost-effective, energy-efficient production of biofuel.
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production of a thermal stress resistant mutant euglena gracilis strain using fe ion beam irradiation
Bioscience Biotechnology and Biochemistry, 2016Co-Authors: Koji Yamada, Yusuke Kazama, Tomoko Abe, S Mitra, Yuka Marukawa, Ryo Arashida, Takahiro Ishikawa, Kengo SuzukiAbstract:Euglena gracilis is a common phytoplankton species, which also has motile flagellate characteristics. Recent research and development has enabled the industrial use of E. gracilis and Selective Breeding of this species is expected to further expand its application. However, the production of E. gracilis nuclear mutants is difficult because of the robustness of its genome. To establish an efficient mutation induction procedure for E. gracilis, we employed Fe-ion beam irradiation in the RIKEN RI beam factory. A decrease in the survival rate was observed with the increase in irradiation dose, and the upper limit used for E. gracilis Selective Breeding was around 50 Gy. For a practical trial of Fe-ion irradiation, we conducted a screening to isolate high-temperature-tolerant mutants. The screening yielded mutants that proliferated faster than the wild-type strain at 32 °C. Our results demonstrate the effectiveness of heavy-ion irradiation on E. gracilis Selective Breeding.
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production of a thermal stress resistant mutant euglena gracilis strain using fe ion beam irradiation
Bioscience Biotechnology and Biochemistry, 2016Co-Authors: Koji Yamada, Sharbanee Mitra, Yusuke Kazama, Yuka Marukawa, Ryo Arashida, Takahiro Ishikawa, Kengo SuzukiAbstract:Euglena gracilis is a common phytoplankton species, which also has motile flagellate characteristics. Recent research and development has enabled the industrial use of E. gracilis and Selective Breeding of this species is expected to further expand its application. However, the production of E. gracilis nuclear mutants is difficult because of the robustness of its genome. To establish an efficient mutation induction procedure for E. gracilis, we employed Fe-ion beam irradiation in the RIKEN RI beam factory. A decrease in the survival rate was observed with the increase in irradiation dose, and the upper limit used for E. gracilis Selective Breeding was around 50 Gy. For a practical trial of Fe-ion irradiation, we conducted a screening to isolate high-temperature-tolerant mutants. The screening yielded mutants that proliferated faster than the wild-type strain at 32 °C. Our results demonstrate the effectiveness of heavy-ion irradiation on E. gracilis Selective Breeding.
Marc Vandeputte - One of the best experts on this subject based on the ideXlab platform.
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The Effects of Feed Restriction and Isolated or Group Rearing on the Measurement of Individual Feed Intake and Estimation of Feed Conversion Ratio in Juvenile Nile Tilapia (Oreochromis niloticus) for Selective Breeding Purposes
Frontiers in Genetics, 2021Co-Authors: Charles Rodde, Marc Vandeputte, Trong Quoc Trinh, Vincent Douchet, Marc Canonne, John Benzie, Hugues De VerdalAbstract:Accurately measuring the phenotype at the individual level is critical to the success of Selective Breeding programs. Feed efficiency is a key sustainability trait and is typically approached through feed conversion ratio (FCR). This requires measurements of body weight gain (BWG) and feed intake (FI), the latter being technically challenging in fish. We assessed two of the principal methods for measuring feed intake in fish over consecutive days: (1) group rearing 10 fish per group and video recording the meals and (2) rearing fish individually on a restricted ration. Juvenile Nile tilapia (Oreochromis niloticus) from the Genetically Improved Farmed Tilapia (GIFT) strain and the Cirad strain were entered into the study (128 GIFT and 109 Cirad). The GIFT strain were reared over three consecutive periods of 7 days each under different feeding, recording, and rearing scenarios (i) in groups fed an optimal ration (g100) or (ii) fed a 50% restricted ration (g50) both with video records of all meals and (iii) reared in isolation and fed a 50% restrictive ration. The Cirad strain were tested similarly but only for scenarios (i) and (iii). All fish were fed twice daily with a calculated ration. Correlations showed the same trends for the GIFT and the Cirad strains. For the GIFT strain, correlations were positive and significant for BWG and FI measured in scenarios (i) and (ii), 0.49 and 0.63, respectively, and FI measured in scenarios (i) and (iii) (0.50) but not for BWG measured in scenarios (i) and (iii) (0.29, NS). The phenotypic correlation estimated for FCR between scenarios (i) and (ii) with fish fed an optimal or a 50% restricted ration was low and not significant (0.22). Feed Conversion Ratio for GIFT fish reared in groups or in isolation and fed with a restricted ration [scenarios (ii) and (iii)] were not significantly correlated either. Social interactions between fish, potentially impacting their efficiency, may explain the results. Therefore, Selective Breeding programs seeking to improve feed efficiency will need to carefully plan the feeding rate and the rearing system used to estimate FCR in order to optimize selection for the targeted production system.
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in search for indirect criteria to improve feed utilization efficiency in sea bass dicentrarchus labrax part ii heritability of weight loss during feed deprivation and weight gain during re feeding periods
Aquaculture, 2010Co-Authors: Beatrice Chatain, Laure Grima, Francois Ruelle, Alain Vergnet, Amandine Launay, Muriel Mambrini, Marc VandeputteAbstract:Selective Breeding to improve residual feed intake (RFI) in sea bass (Dicentrarchus labrax) is a major goal that would optimize economic gain while minimizing the environmental impact of production. Due to the difficulty in accurately measuring individual feed intake, no Selective Breeding program has yet been started. In a previous study, we identified a criterion phenotypically related to RFI variations: the loss of weight during feed deprivation, FD. Moreover, an additional composite criterion (CC) integrating both FD and weight gain during subsequent re-feeding (RF) was closely related to RFI, even though the relationship was only close to significance (P = 0.06). The aim of the present study was to estimate the heritability of these two traits, in order to complete the analysis of their pertinence as indirect criteria for use in a Selective Breeding program to improve RFI. We set up a full factorial mating design combining 41 sires and eight dams to produce mixed families offspring, which were all raised in the same tank. At 50 g, 1300 individuals were individually tagged and a fin clip was sampled for DNA extraction and parentage reassignment using five to six microsatellite loci, which showed they originated from 261 families. The evolution of individual body weight was recorded during a growth period of three weeks, followed by successive periods of three weeks feed deprivation and three weeks ad libitum re-feeding, repeated twice. Phenotypic and genetic correlations between the two feed deprivation periods or the two re-feeding periods were moderate (r = 0.45–0.51, and 0.71–0.73 respectively for phenotypic and genetic correlations) indicating that FD and RF are relatively repeatable. FD, RF and CC heritabilities were of the same magnitude (0.23 ± 0.04, 0.19 ± 0.04 and 0.22 ± 0.04, respectively), and sufficient to consider the use of such criteria in a future Breeding program.
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heritability estimates for growth related traits using microsatellite parentage assignment in juvenile common carp cyprinus carpio l
Aquaculture, 2004Co-Authors: Marc Vandeputte, Martin Kocour, Stephane Mauger, Mathilde Dupontnivet, Daphne De Guerry, M Rodina, D Gela, Dominique Vallod, Bernard Chevassus, Otomar LinhartAbstract:Abstract Common carp is one of the leading species in world aquaculture, but Selective Breeding for growth rate has not been actively pursued in this species after unsuccessful selection experiments. We estimated heritability for growth-related traits at 8 weeks of age in Hungarian Synthetic Mirror carp at Vodnany (Czech Republic). Parentage assignment with eight microsatellite markers was used in a full factorial cross of 10 dams×24 sires. Out of 550 offspring, 95.3% could be assigned to a single parental pair. Animal model heritability estimates were 0.33±0.08 for weight 0.33±0.07 for length and 0.37±0.07 for Fulton's condition factor ( K ). Maternal effects and dominance were not significantly different from zero. The genetic correlation between weight and length was 0.98, and negative correlations were found between K and length (−0.38) and K and weight (−0.17). It is concluded that Selective Breeding for increased weight gain can be successful in juvenile carp, using indirect selection for length. However, the facts that heritability was estimated at 8 weeks of age and not at harvest weight, as well as a possible amplification of additive variance by competition, are limitations to the applicability of the present results.
Sharbanee Mitra - One of the best experts on this subject based on the ideXlab platform.
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Osamu IwataAbstract:Efficient Selective Breeding of live oil-rich Euglena gracilis with fluorescence-activated cell sorting
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efficient Selective Breeding of live oil rich euglena gracilis with fluorescence activated cell sorting
Scientific Reports, 2016Co-Authors: Koji Yamada, Takuto Takeuchi, Sharbanee Mitra, Yusuke Kazama, Keisuke Goda, Kengo Suzuki, Hideyuki Suzuki, Tomoko Abe, Osamu IwataAbstract:Euglena gracilis, a microalgal species of unicellular flagellate protists, has attracted much attention in both the industrial and academic sectors due to recent advances in the mass cultivation of E. gracilis that have enabled the cost-effective production of nutritional food and cosmetic commodities. In addition, it is known to produce paramylon (β-1,3-glucan in a crystalline form) as reserve polysaccharide and convert it to wax ester in hypoxic and anaerobic conditions-a promising feedstock for biodiesel and aviation biofuel. However, there remain a number of technical challenges to be solved before it can be deployed in the competitive fuel market. Here we present a method for efficient Selective Breeding of live oil-rich E. gracilis with fluorescence-activated cell sorting (FACS). Specifically, the Selective Breeding method is a repetitive procedure for one-week heterotrophic cultivation, staining intracellular lipids with BODIPY(505/515), and FACS-based isolation of top 0.5% lipid-rich E. gracilis cells with high viability, after inducing mutation with Fe-ion irradiation to the wild type (WT). Consequently, we acquire a live, stable, lipid-rich E. gracilis mutant strain, named B1ZFeL, with 40% more lipid content on average than the WT. Our method paves the way for rapid, cost-effective, energy-efficient production of biofuel.
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production of a thermal stress resistant mutant euglena gracilis strain using fe ion beam irradiation
Bioscience Biotechnology and Biochemistry, 2016Co-Authors: Koji Yamada, Sharbanee Mitra, Yusuke Kazama, Yuka Marukawa, Ryo Arashida, Takahiro Ishikawa, Kengo SuzukiAbstract:Euglena gracilis is a common phytoplankton species, which also has motile flagellate characteristics. Recent research and development has enabled the industrial use of E. gracilis and Selective Breeding of this species is expected to further expand its application. However, the production of E. gracilis nuclear mutants is difficult because of the robustness of its genome. To establish an efficient mutation induction procedure for E. gracilis, we employed Fe-ion beam irradiation in the RIKEN RI beam factory. A decrease in the survival rate was observed with the increase in irradiation dose, and the upper limit used for E. gracilis Selective Breeding was around 50 Gy. For a practical trial of Fe-ion irradiation, we conducted a screening to isolate high-temperature-tolerant mutants. The screening yielded mutants that proliferated faster than the wild-type strain at 32 °C. Our results demonstrate the effectiveness of heavy-ion irradiation on E. gracilis Selective Breeding.
