The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Madan M Gupta - One of the best experts on this subject based on the ideXlab platform.
-
liquid chromatographic determination of Sennosides in cassia angustifolia leaves
Journal of AOAC International, 2006Co-Authors: Alpana Srivastava, Richa Pandey, R K Verma, Madan M GuptaAbstract:: A simple liquid chromatographic method was developed for the determination of Sennosides B and A in leaves of Cassia angustifolia. These compounds were extracted from leaves with a mixture of methanol-water (70 + 30, v/v) after defatting with hexane. Analyte separation and quantitation were achieved by gradient reversed-phase liquid chromatography and UV absorbance at 270 nm using a photodiode array detector. The method involves the use of an RP-18 Lichrocart reversed-phase column (5 microm, 125 x 4.0 mm id) and a binary gradient mobile-phase profile. The various other aspects of analysis, namely, peak purity, similarity, recovery, repeatability, and robustness, were validated. Average recoveries of 98.5 and 98.6%, with a coefficient of variation of 0.8 and 0.3%, were obtained by spiking sample solution with 3 different concentration solutions of standards (60, 100, and 200 microg/mL). Detection limits were 10 microg/mL for Sennoside B and 35 microg/mL for Sennoside A, present in the sample solution. The quantitation limits were 28 and 100 microg/mL. The analytical method was applied to a large number of senna leaf samples. The new method provides a reliable tool for rapid screening of C. angustifolia samples in large numbers, which is needed in breeding/genetic engineering and genetic mapping experiments.
-
reverse phase high performance liquid chromatography of Sennosides in cassia angustifolia
Phytochemical Analysis, 1996Co-Authors: R K Verma, G C Uniyal, S P Singh, J R Sharma, Madan M GuptaAbstract:A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles Sennoside A and Sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.
R K Verma - One of the best experts on this subject based on the ideXlab platform.
-
liquid chromatographic determination of Sennosides in cassia angustifolia leaves
Journal of AOAC International, 2006Co-Authors: Alpana Srivastava, Richa Pandey, R K Verma, Madan M GuptaAbstract:: A simple liquid chromatographic method was developed for the determination of Sennosides B and A in leaves of Cassia angustifolia. These compounds were extracted from leaves with a mixture of methanol-water (70 + 30, v/v) after defatting with hexane. Analyte separation and quantitation were achieved by gradient reversed-phase liquid chromatography and UV absorbance at 270 nm using a photodiode array detector. The method involves the use of an RP-18 Lichrocart reversed-phase column (5 microm, 125 x 4.0 mm id) and a binary gradient mobile-phase profile. The various other aspects of analysis, namely, peak purity, similarity, recovery, repeatability, and robustness, were validated. Average recoveries of 98.5 and 98.6%, with a coefficient of variation of 0.8 and 0.3%, were obtained by spiking sample solution with 3 different concentration solutions of standards (60, 100, and 200 microg/mL). Detection limits were 10 microg/mL for Sennoside B and 35 microg/mL for Sennoside A, present in the sample solution. The quantitation limits were 28 and 100 microg/mL. The analytical method was applied to a large number of senna leaf samples. The new method provides a reliable tool for rapid screening of C. angustifolia samples in large numbers, which is needed in breeding/genetic engineering and genetic mapping experiments.
-
reverse phase high performance liquid chromatography of Sennosides in cassia angustifolia
Phytochemical Analysis, 1996Co-Authors: R K Verma, G C Uniyal, S P Singh, J R Sharma, Madan M GuptaAbstract:A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles Sennoside A and Sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.
J R Sharma - One of the best experts on this subject based on the ideXlab platform.
-
reverse phase high performance liquid chromatography of Sennosides in cassia angustifolia
Phytochemical Analysis, 1996Co-Authors: R K Verma, G C Uniyal, S P Singh, J R Sharma, Madan M GuptaAbstract:A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles Sennoside A and Sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.
S P Singh - One of the best experts on this subject based on the ideXlab platform.
-
reverse phase high performance liquid chromatography of Sennosides in cassia angustifolia
Phytochemical Analysis, 1996Co-Authors: R K Verma, G C Uniyal, S P Singh, J R Sharma, Madan M GuptaAbstract:A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles Sennoside A and Sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.
G C Uniyal - One of the best experts on this subject based on the ideXlab platform.
-
reverse phase high performance liquid chromatography of Sennosides in cassia angustifolia
Phytochemical Analysis, 1996Co-Authors: R K Verma, G C Uniyal, S P Singh, J R Sharma, Madan M GuptaAbstract:A rapid and simple reverse-phase high performance liquid chromatographic method has been developed for the quantitative estimation of the medicinally useful laxative principles Sennoside A and Sennoside B in senna. Using a Bondapak C,, column eluted with methano1:water:acetic acid:tetrahydrofuran (60:38:2:2) both compounds were well resolved with recoveries of 97 and 96% respectively.
