The Experts below are selected from a list of 267 Experts worldwide ranked by ideXlab platform
Narayana M. S. Sirimuthu - One of the best experts on this subject based on the ideXlab platform.
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Surface-enhanced Raman spectroscopy (SERS) for sub-micromolar detection of DNA/RNA mononucleotides.
Journal of the American Chemical Society, 2006Co-Authors: Steven E. J. Bell, Narayana M. S. SirimuthuAbstract:Surface-enhanced Raman (SER) spectra of all the DNA/RNA mononucleotides have been obtained with high Sensitivity using citrate-reduced silver colloids aggregated with MgSO4, rather than the more usual halide ions, which were found to prevent enhancement of these compounds. The SERS spectra of adenine, guanine, thymine, cytosine, and uracil were recorded along with their corresponding nucleosides and 5'-deoxynucleotides. For the cytosine series, all three spectra had similar relative band intensities but the spectra of adenine were different from those of adenosine and dAMP, probably due to differences in orientation on the surface. No enhanced bands from the phosphate or sugar groups were observed. There were general similarities between the SERS spectra of the purine mononucleotides and the pyrimidine mononucleotides, but the spectra were sufficiently different to allow each of them to be distinguished. This method can therefore be used for high Sensitivity, Label-free identification of mononucleotides.
M. Hedeșiu - One of the best experts on this subject based on the ideXlab platform.
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Surface-enhanced Raman spectroscopy (SERS) investigations of saliva for oral cancer diagnosis
Lasers in Medical Science, 2020Co-Authors: A. Fălămaș, H. Rotaru, M. HedeșiuAbstract:Saliva could be an optimal sample for non-invasive cancer detection, as it contains plenty of proteins and metabolites which can reflect the health status of an individual. Moreover, pairing it with high-Sensitivity, Label-free detection techniques could prove successful for early cancer diagnosis. In this study, we explore the enhancement of salivary characteristic Raman bands by using Label-free, ultrasensitive surface-enhanced Raman scattering (SERS) based on gold nanoparticles. SERS maps were acquired from dry samples of saliva supernatant mixed with Au colloidal nanoparticles, which was then pipetted on clean glass slides. The SERS spectra presented a high variability of signal intensities and frequency shifts. However, several reproducible SERS spectra showing well-resolved bands were obtained at certain locations on the maps, where Au nanoparticles clustered together during the air-drying. The healthy and oral cancer saliva could be differentiated using principal components analysis based on several SERS bands assigned mainly to amino acids and proteins. Moreover, thiocyanate Raman modes were detected in saliva samples of both smoking and non-smoking volunteers and cancer patients. The analysis indicated that the cancer group displayed an overall higher level of the 2126 cm^−1 band area assigned to C–N stretching vibrations of thiocyanate.
Li-yang Shao - One of the best experts on this subject based on the ideXlab platform.
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Ultrahigh-Sensitivity Label-free optical fiber biosensor based on a tapered singlemode- no core-singlemode coupler for Staphylococcus aureus detection
Sensors and Actuators B: Chemical, 2020Co-Authors: Chen Ling, Yuankui Leng, Bin Liu, Juan Liu, Wan Shengpeng, Jinhui Yuan, Li-yang ShaoAbstract:Abstract An ultra-high Sensitivity Label-free optical fiber biosensor for inactivated Staphylococcus aureus (S. aureus) detection is proposed and investigated in this study, with additional advantages of robust and stability compared to traditional tapered fiber structure. The proposed fiber biosensor is based on a tapered singlemode- no core-singlemode fiber coupler (SNSFC) structure, where the no core fiber was tapered to small diameter (taper-waist diameter of about 10 μm) and functionalized with the pig immunoglobulin G (IgG) antibody for detection of S. aureus. The measured maximum wavelength shift of the sensor for an S. aureus concentration of 7 × 101 CFU/mL (colony forming unit per milliliter) is 2.04 nm, which is equivalent to a limit of detection (LOD) of 3.1 CFU/mL (a highest LOD reported so far for optical fiber biosensors), considering the maximum wavelength variation of the sensor in phosphate buffered saline (PBS) is ±0.03 nm over 40 min, where 3 times of maximum wavelength variation (3 × 0.03 = 0.09 nm) is defined as measurement limit. The response time of the developed fiber sensor is less than 30 min. The ultra-sensitive biosensor has potential to be widely applied to various areas such as disease, medical diagnostics and food safety inspection.
Connie J Changhasnain - One of the best experts on this subject based on the ideXlab platform.
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ultracompact high Sensitivity Label free biosensor using vcsel
Biomedical optics, 2004Co-Authors: C.f.r. Mateus, Michael C.y. Huang, Jonathan E. Foley, Brian T. Cunningham, Robert P Beatty, Connie J ChanghasnainAbstract:We report a Label-free, highly sensitive biosensor using a vertical cavity surface emitting laser (VCSEL) based measurement system for the detection and monitoring of biomolecular interactions. The sensor system consists of a VCSEL, a plastic guided mode resonant (GMR) filter, and two pin detectors. The system has several advantages such as extreme compactness, high Sensitivity, high throughput, fast measurements, low power consumption, low cost, and the potential to become portable. Experimentally, the biosensor system has shown to be highly sensitive to the surface modifications due to molecular bindings, with the ability to detect the thickness variations <10a and refractive index variations <0.005. The biosensor also has demonstrated its high Sensitivity for the detection of antibody-antigen proteins bindings, with the mouse IgG concentration as low as 1pg/ml (6.7 femto-Molar), and its ability for measuring both static and dynamic interactions among proteins.
C.f.r. Mateus - One of the best experts on this subject based on the ideXlab platform.
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Ultracompact high-Sensitivity Label-free biosensor using VCSEL
Microarrays and Combinatorial Techniques: Design Fabrication and Analysis II, 2004Co-Authors: C.f.r. Mateus, Michael C.y. Huang, Jonathan E. Foley, P. Robert Beatty, Brian T. Cunningham, Connie J. Chang-hasnainAbstract:We report a Label-free, highly sensitive biosensor using a vertical cavity surface emitting laser (VCSEL) based measurement system for the detection and monitoring of biomolecular interactions. The sensor system consists of a VCSEL, a plastic guided mode resonant (GMR) filter, and two pin detectors. The system has several advantages such as extreme compactness, high Sensitivity, high throughput, fast measurements, low power consumption, low cost, and the potential to become portable. Experimentally, the biosensor system has shown to be highly sensitive to the surface modifications due to molecular bindings, with the ability to detect the thickness variations
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ultracompact high Sensitivity Label free biosensor using vcsel
Biomedical optics, 2004Co-Authors: C.f.r. Mateus, Michael C.y. Huang, Jonathan E. Foley, Brian T. Cunningham, Robert P Beatty, Connie J ChanghasnainAbstract:We report a Label-free, highly sensitive biosensor using a vertical cavity surface emitting laser (VCSEL) based measurement system for the detection and monitoring of biomolecular interactions. The sensor system consists of a VCSEL, a plastic guided mode resonant (GMR) filter, and two pin detectors. The system has several advantages such as extreme compactness, high Sensitivity, high throughput, fast measurements, low power consumption, low cost, and the potential to become portable. Experimentally, the biosensor system has shown to be highly sensitive to the surface modifications due to molecular bindings, with the ability to detect the thickness variations <10a and refractive index variations <0.005. The biosensor also has demonstrated its high Sensitivity for the detection of antibody-antigen proteins bindings, with the mouse IgG concentration as low as 1pg/ml (6.7 femto-Molar), and its ability for measuring both static and dynamic interactions among proteins.
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Ultra compact, high Sensitivity Label-free biosensor using VCSEL
2003Co-Authors: C.f.r. Mateus, Brian T. Cunningham, Connie J. Chang-hasnain, J.-s.p. Hung, H. Yao, G. Hasnain, C. Kuo, A. LiaoAbstract:We report a new Label-free biosensor system for protein interaction characterization. The system consists of a VCSEL, a plastic guided mode resonant filter, and a pin detector. The sensor has high Sensitivity and ultra compactness, consumes low power, and can be fabricated in 2D arrays.
