The Experts below are selected from a list of 207 Experts worldwide ranked by ideXlab platform
Raymond Negrel - One of the best experts on this subject based on the ideXlab platform.
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Prostacyclin as an Indicator of Preadipocyte Transformation: Studies in Vivo by Microdialysis and in Vitro
Cancer research, 1994Co-Authors: Christian Darimont, Gérard Ailhaud, Raymond NegrelAbstract:Nontransformed (Ob1771) and polyoma virus-transformed (Ob17PY) mouse cells from the preadipocyte Ob17 clonal line have been compared in their ability to release prostaglandins in vitro as well as in vivo as assayed by in situ microdialysis. Prostaglandin FE2, prostaglandin-2 alpha and mainly prostacyclin are released in larger amounts (4- to 10-fold) by Ob17PY cells in vitro and Ob17PY-induced tumors in vivo as compared to Ob1771 preadipocytes in vitro and periepididymal adipose tissue in vivo. In contrast to Ob1771 preadipocytes, none of these prostanoids appear to be involved in the control of proliferation or differentiation of Ob17PY cells in Serum-Free Culture Medium. However, prostacyclin, the level of which is the most affected by transformation, might be considered as a valuable indicator of fibrosarcoma development.
Alicia Roldán - One of the best experts on this subject based on the ideXlab platform.
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Role of insulin‐like growth factor‐1 on the kinetics of human lymphocytes stimulation in serum‐free Culture Medium
Immunology and cell biology, 1994Co-Authors: Roxana Schillaci, Claudia M. Ribaudo, Cristina M. Rondinone, Alicia RoldánAbstract:Role of insulin-like growth factor-1 on the kinetics of human lymphocytes stimulation in Serum-Free Culture Medium
Yan Wang - One of the best experts on this subject based on the ideXlab platform.
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Human umbilical cord stem cell conditioned Medium versus Serum-Free Culture Medium in the treatment of cryopreserved human ovarian tissues in in-vitro Culture: a randomized controlled trial.
Stem cell research & therapy, 2017Co-Authors: Yingxian Jia, Xiaohan Shi, Yidong Xie, Xiaochuan Xie, Yan WangAbstract:To reduce young female fertility loss, the in-vitro Culture of cryopreserved ovarian cortical tissues (OCTs) is considered an effective approach without delaying treatment and undergoing stimulation medicine. However, ischemic damage and follicular loss during the in-vitro Culture of OCTs are major technical challenges. Human umbilical cord stem cells (HUMSCs) and their conditioned Medium (HUMSC-CM) have been considered to be potential resources for regeneration medicine because they secrete cytokines and enhance cell survival and function. The aim of this study was to determine whether HUMSC-CM improves the development of frozen-thawed in-vitro Cultured ovarian tissues compared with a Serum-Free Culture Medium (SF-CM). The thawed OCTs (n = 68) were cultivated in HUMSC-CM and SF-CM in vitro for 8 days, and the ovarian tissues were processed and analyzed by a classical histological evaluation. The microvessel density (MVD) and apotosis detection during in-vitro Culture of OCTs were also performed. A significant difference in the rate of morphologically normal primordial follicles in the HUMSC-CM group was observed compared to that in the SF-CM group (group C) from days 2 to 4 (day 2: group B 58.0 ± 2.45% vs group C 32.0 ± 5.83%, p = 0.002; day 3: group B 55.5 ± 4.20% vs group C 21.0 ± 9.80%, p = 0.048; day 4: group B 52.0 ± 4.08% vs group C 21.5 ± 8.19%, p = 0.019). The microvessel density (MVD) detection showed a time-dependent increase and peaked on day 4. There was a significant difference between groups B (49.33 ± 0.58) and C (24.33 ± 3.79) (p = 0.036). The percentage of apoptotic follicles in group B was lower than that in group C on day 1 (13.75 ± 2.50% vs 27.0 ± 10.10%, p = 0.003), day 5 (11.75 ± 1.50% vs 51.0 ± 10.5%, p = 0.019) and day 7 (15.0 ± 5.10% vs 46.5 ± 21.75%, p = 0.018). These data have provided the first experimental evidence of the effect of HUMSC-CM on frozen-thawed OCTs in vitro. The results showed that the HUMSC-CM group provided a better protecting effect on the in-vitro Culture of the cryopreserved OCTs compared to the SF-CM group.
Christian Darimont - One of the best experts on this subject based on the ideXlab platform.
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Prostacyclin as an Indicator of Preadipocyte Transformation: Studies in Vivo by Microdialysis and in Vitro
Cancer research, 1994Co-Authors: Christian Darimont, Gérard Ailhaud, Raymond NegrelAbstract:Nontransformed (Ob1771) and polyoma virus-transformed (Ob17PY) mouse cells from the preadipocyte Ob17 clonal line have been compared in their ability to release prostaglandins in vitro as well as in vivo as assayed by in situ microdialysis. Prostaglandin FE2, prostaglandin-2 alpha and mainly prostacyclin are released in larger amounts (4- to 10-fold) by Ob17PY cells in vitro and Ob17PY-induced tumors in vivo as compared to Ob1771 preadipocytes in vitro and periepididymal adipose tissue in vivo. In contrast to Ob1771 preadipocytes, none of these prostanoids appear to be involved in the control of proliferation or differentiation of Ob17PY cells in Serum-Free Culture Medium. However, prostacyclin, the level of which is the most affected by transformation, might be considered as a valuable indicator of fibrosarcoma development.
Gaetano Mari - One of the best experts on this subject based on the ideXlab platform.
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EFFECT OF PROGESTERONE AND EPIDERMAL GROWTH FACTOR ON IN VITRO-PRODUCED EIGHT-CELL BOVINE EMBRYOS IN Serum-Free Culture Medium
Reproduction Fertility and Development, 2007Co-Authors: Barbara Merlo, Eleonora Iacono, Gaetano MariAbstract:The role of progesterone (P4) and epidermal growth factor (EGF) in early bovine embryo development is still not clear. P4 has been administered at different times of embryo development, and a direct effect on IVF-derived bovine 8-cell embryos has been noted even if there was an interference due to the P4 vehicle (Ferguson et al. 2005 Reprod. Fertil. Dev. 17, 219 abst). EGF has been added to the Culture Medium from the presumptive zygote stage at different concentrations, resulting in improved blastocyst rates compared to that in control Medium (Mtango et al. 2003 Theriogenology 59, 1393–1402; Sirisathien et al. 2003 Anim. Reprod. Sci. 77, 21–32), and gave results similar to those with 5% or 10% FCS (Palasz et al. 2000 Anim. Reprod. Sci. 58, 229–240). The objective if this experiment was to determine the effect of P4 and EGF on development of in vitro-produced bovine embryos when administered alone or in combination at the 8-cell stage in the absence of serum. In vitro-produced bovine 8-cell embryos were randomly allotted to treatments: (1) control, SOFaaBSA Medium (BSA, 16 mg mL−1; n = 198); (2) P4, SOFaaBSA + P4 (15 ng mL−1 in ethanol; n = 198); (3) EGF, SOFaaBSA + EGF (25 ng mL−1; n = 200); (4) P4 + EGF, SOFaaBSA + P4 (15 ng mL−1 in ethanol) + EGF (25 ng mL−1; n = 201); and (5) FBS, SOFaaBSA + FBS (5%; n = 197). In order to minimize the toxic effect of ethanol, it was allowed to evaporate from the Culture dish and then Medium was added. All in vitro procedures were carried out at 38.5°C in a humidified atmosphere of 5% CO2 in air; presumptive zygotes were Cultured in SOFaaBSA until 8-cell stage. Embryo development was evaluated on Day 6 and on Day 8 after IVF (Day 0), and rates calculated from 8-cell embryos. The study was done in 4 replicates and chi-square test was used for statistical analysis (Statistica for Windows; Stat Soft Inc., Tulsa, OK, USA); significance was assessed at P 0.05), whereas the combination P4 + EGF was better than P4 alone (P 0.05) among EGF, P4 + EGF, and FBS groups. P4 achieved an higher (P < 0.05) blastocyst rate than control but it was lower (P < 0.05) than that of P4 + EGF or FBS. In conclusion, P4 alone improves embryo development from the 8-cell embryo to the blastocyst stage in a Serum-Free Culture system, and EGF alone achieves a blastocyst rate not significantly different from that of FBS; furthermore, the combination of P4 and EGF can be considered the most suitable as an alternative to FBS because similar results were obtained in terms of both morulae and blastocysts. Table 1.Eight-cell bovine embryo development in SOFaaBSA Medium in presence of P4, EGF, P4+EGF, or FBS
