The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform
Raúl S. Lavado - One of the best experts on this subject based on the ideXlab platform.
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Dark septate endophytes present different potential to solubilize calcium, iron and aluminum phosphates
Applied Soil Ecology, 2017Co-Authors: Federico N. Spagnoletti, N. Tobar, A. Fernández Di Pardo, Viviana Monica Chiocchio, Raúl S. LavadoAbstract:Many microorganisms play a significant role in releasing phosphorus (P) from soil insoluble phosphates to crops. Here, we evaluated the ability of dark septate endophytes (DSE) to solubilize calcium, aluminum and iron phosphates. DSE were isolated from the roots of wheat (Triticum aestivum) and the forages Panicum coloratum and Chloris gayana, which are grown in slightly acidic and alkaline soils of the Argentine Pampas, respectively. Protocols to corroborate their endophytic nature were followed. Nine fungi were identified by morphological and molecular characteristics, and their sequences were deposited in GenBank. The isolates belonged to the same order and genera as DSE fungi recorded in other parts of the world. The temperature and pH requirements of the DSE strains were verified. To determine their ability to solubilize phosphate, we followed two in vitro methodologies: solid and liquid media. On solid medium, all isolates showed ability to solubilize calcium phosphate, three strains solubilized aluminum phosphate, and none of them solubilized iron phosphate. The DSE most efficient in solubilizing calcium phosphate were Ophiosphaerella sp. and Cochliobolus sp., followed by Setosphaeria rostrata. The strains Drechslera sp. (P6), Ophiosphaerella herpotricha and Drechslera sp. (12–15) were able to solubilize aluminum phosphate. In liquid medium, the isolates showed different ability to generate acidity and to solubilize phosphates. Drechslera sp. (12–15) was among the most efficient in solubilizing calcium phosphate, Curvularia sp. in solubilizing aluminum phosphate and Ophiosphaerella sp. in solubilizing iron phosphate. The results obtained combining both methodologies indicate that S. rostrata was not the best with each phosphate individually but showed the best global performance. DSE fungi are far less identified than other groups of fungi and bacteria as soil insoluble phosphate-solubilizing agents. However, they showed potential for application as biofertilizers in different soils to manage sustainable agroecosystems.
Allal Douira - One of the best experts on this subject based on the ideXlab platform.
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l hibiscus rosa sinensis un nouvel hote pour le cochliobolus spicifer et le Setosphaeria rostrata
Phytoprotection, 2008Co-Authors: Nabila Meddah, Amina Ouazzani Touhami, Allal DouiraAbstract:Au Maroc, le sol et le climat sont deux facteurs ayant encourage le developpement des cultures ornementales. Or, la situation actuelle du secteur ornemental est telle que l'entretien et le diagnostic phytosanitaire sont loin d'etre pratiques regulierement. Des isolements fongiques a partir des lesions foliaires de l'Hibiscus rosa-sinensis ont revele pour la premiere fois la presence du Setosphaeria rostrata et du Cochliobolus spicifer. Ces deux especes induisent des symptomes foliaires differents une fois inoculees aux plantes de l'Hibiscus rosa-sinensis. Le S. rostrata provoque des lesions de forme irreguliere et de couleur brun clair ou noirâtre et entraine un dessechement du limbe. Le C. spicifer est responsable de lesions foliaires de couleur brun clair qui apparaissent a la marge des feuilles puis se developpent vers le centre et prennent un contour pâle. Le C. spicifer est l'agent pathogene le plus agressif (coefficient d'infection = 44).
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L'Hibiscus rosa-sinensis, un nouvel hôte pour le Cochliobolus spicifer et le Setosphaeria rostrata
Communication brève, 2007Co-Authors: Nabila Meddah, Amina Ouazzani Touhami, Allal DouiraAbstract:Au Maroc, le sol et le climat sont deux facteurs ayant encouragé le développement des cultures ornementales. Or, la situation actuelle du secteur ornemental est telle que l'entretien et le diagnostic phytosanitaire sont loin d'être pratiqués régulièrement. Des isolements fongiques à partir des lésions foliaires de l'Hibiscus rosa-sinensisont révélé pour la première fois la présence duSetosphaeria rostrataet duCochliobolus spicifer. Ces deux espèces induisent des symptômes foliaires différents une fois inoculées aux plantes de l'Hibiscus rosa-sinensis. LeS. rostrataprovoque des lésions de forme irrégulière et de couleur brun clair ou noirâtre et entraîne un dessèchement du limbe. LeC. spiciferest responsable de lésions foliaires de couleur brun clair qui apparaissent à la marge des feuilles puis se développent vers le centre et prennent un contour pâle. LeC. spiciferest l'agent pathogène le plus agressif (coefficient d'infection = 44).
Federico N. Spagnoletti - One of the best experts on this subject based on the ideXlab platform.
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Dark septate endophytes present different potential to solubilize calcium, iron and aluminum phosphates
Applied Soil Ecology, 2017Co-Authors: Federico N. Spagnoletti, N. Tobar, A. Fernández Di Pardo, Viviana Monica Chiocchio, Raúl S. LavadoAbstract:Many microorganisms play a significant role in releasing phosphorus (P) from soil insoluble phosphates to crops. Here, we evaluated the ability of dark septate endophytes (DSE) to solubilize calcium, aluminum and iron phosphates. DSE were isolated from the roots of wheat (Triticum aestivum) and the forages Panicum coloratum and Chloris gayana, which are grown in slightly acidic and alkaline soils of the Argentine Pampas, respectively. Protocols to corroborate their endophytic nature were followed. Nine fungi were identified by morphological and molecular characteristics, and their sequences were deposited in GenBank. The isolates belonged to the same order and genera as DSE fungi recorded in other parts of the world. The temperature and pH requirements of the DSE strains were verified. To determine their ability to solubilize phosphate, we followed two in vitro methodologies: solid and liquid media. On solid medium, all isolates showed ability to solubilize calcium phosphate, three strains solubilized aluminum phosphate, and none of them solubilized iron phosphate. The DSE most efficient in solubilizing calcium phosphate were Ophiosphaerella sp. and Cochliobolus sp., followed by Setosphaeria rostrata. The strains Drechslera sp. (P6), Ophiosphaerella herpotricha and Drechslera sp. (12–15) were able to solubilize aluminum phosphate. In liquid medium, the isolates showed different ability to generate acidity and to solubilize phosphates. Drechslera sp. (12–15) was among the most efficient in solubilizing calcium phosphate, Curvularia sp. in solubilizing aluminum phosphate and Ophiosphaerella sp. in solubilizing iron phosphate. The results obtained combining both methodologies indicate that S. rostrata was not the best with each phosphate individually but showed the best global performance. DSE fungi are far less identified than other groups of fungi and bacteria as soil insoluble phosphate-solubilizing agents. However, they showed potential for application as biofertilizers in different soils to manage sustainable agroecosystems.
Nabila Meddah - One of the best experts on this subject based on the ideXlab platform.
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l hibiscus rosa sinensis un nouvel hote pour le cochliobolus spicifer et le Setosphaeria rostrata
Phytoprotection, 2008Co-Authors: Nabila Meddah, Amina Ouazzani Touhami, Allal DouiraAbstract:Au Maroc, le sol et le climat sont deux facteurs ayant encourage le developpement des cultures ornementales. Or, la situation actuelle du secteur ornemental est telle que l'entretien et le diagnostic phytosanitaire sont loin d'etre pratiques regulierement. Des isolements fongiques a partir des lesions foliaires de l'Hibiscus rosa-sinensis ont revele pour la premiere fois la presence du Setosphaeria rostrata et du Cochliobolus spicifer. Ces deux especes induisent des symptomes foliaires differents une fois inoculees aux plantes de l'Hibiscus rosa-sinensis. Le S. rostrata provoque des lesions de forme irreguliere et de couleur brun clair ou noirâtre et entraine un dessechement du limbe. Le C. spicifer est responsable de lesions foliaires de couleur brun clair qui apparaissent a la marge des feuilles puis se developpent vers le centre et prennent un contour pâle. Le C. spicifer est l'agent pathogene le plus agressif (coefficient d'infection = 44).
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L'Hibiscus rosa-sinensis, un nouvel hôte pour le Cochliobolus spicifer et le Setosphaeria rostrata
Communication brève, 2007Co-Authors: Nabila Meddah, Amina Ouazzani Touhami, Allal DouiraAbstract:Au Maroc, le sol et le climat sont deux facteurs ayant encouragé le développement des cultures ornementales. Or, la situation actuelle du secteur ornemental est telle que l'entretien et le diagnostic phytosanitaire sont loin d'être pratiqués régulièrement. Des isolements fongiques à partir des lésions foliaires de l'Hibiscus rosa-sinensisont révélé pour la première fois la présence duSetosphaeria rostrataet duCochliobolus spicifer. Ces deux espèces induisent des symptômes foliaires différents une fois inoculées aux plantes de l'Hibiscus rosa-sinensis. LeS. rostrataprovoque des lésions de forme irrégulière et de couleur brun clair ou noirâtre et entraîne un dessèchement du limbe. LeC. spiciferest responsable de lésions foliaires de couleur brun clair qui apparaissent à la marge des feuilles puis se développent vers le centre et prennent un contour pâle. LeC. spiciferest l'agent pathogène le plus agressif (coefficient d'infection = 44).
Azmi Abd. Razak - One of the best experts on this subject based on the ideXlab platform.
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Morphological and molecular characterization, sexual reproduction, and pathogenicity of Setosphaeria rostrata isolates from rice leaf spot.
Mycologia, 2016Co-Authors: Nor Azizah Kusai, Madihah Mior Zakuan Azmi, Nur Ain Izzati Mohd Zainudin, Mohd Termizi Yusof, Azmi Abd. RazakAbstract:Setosphaeria rostrata, a common plant pathogen causing leaf spot disease, affects a wide range of plant species, mainly grasses. Fungi were isolated from brown spots on rice leaves throughout Peninsular Malaysia, and 45 isolates were identified as Setosphaeria rostrata The isolates were then characterized using morphological and molecular approaches. The mating type was determined using PCR amplification of the mating type alleles, and isolates of opposite mating types were crossed to examine sexual reproduction. Based on nuclear ribosomal DNA ITS1-5.8S-ITS2 region (ITS) and beta-tubulin (BT2) sequences, two phylogenetic trees were constructed using the maximum likelihood method; S. rostrata was clustered in one well-supported clade. Pathogenicity tests showed that S. rostrata isolates are pathogenic, suggesting that it is the cause of the symptoms. Mating-type analyses indicated that three isolates carried the MAT1-1 allele, and the other 42 isolates carried MAT1-2 After isolates with opposite mating types were crossed on Sach's medium and incubated for 3 wk, six crosses produced pseudothecia that contained eight mature ascospores, and 12 other crosses produced numerous pseudothecia with no ascospores. To our knowledge, this is the first report on S. rostrata isolated from leaf spots on rice.